The Regulation of Photosynthetic Structure and Function during Nitrogen Deprivation in Chlamydomonas reinhardtii
Abstract
The accumulation of carbon storage compounds by many unicellular algae after nutrient deprivation occurs despite declines in their photosynthetic apparatus. To understand the regulation and roles of photosynthesis during this potentially bioenergetically valuable process, we analyzed photosynthetic structure and function after nitrogen deprivation in the model alga Chlamydomonas reinhardtii. Transcriptomic, proteomic, metabolite, and lipid profiling and microscopic time course data were combined with multiple measures of photosynthetic function. Levels of transcripts and proteins of photosystems I and II and most antenna genes fell with differing trajectories; thylakoid membrane lipid levels decreased, while their proportions remained similar and thylakoid membrane organization appeared to be preserved. Cellular chlorophyll (Chl) content decreased more than 2-fold within 24 h, and we conclude from transcript protein and 13C labeling rates that Chl synthesis was down-regulated both pre- and posttranslationally and that Chl levels fell because of a rapid cessation in synthesis and dilution by cellular growth rather than because of degradation. Photosynthetically driven oxygen production and the efficiency of photosystem II as well as P700+ reduction and electrochromic shift kinetics all decreased over the time course, without evidence of substantial energy overflow. The results also indicate that linear electron flow fell approximately 15% more thanmore »
- Authors:
-
- Michigan State Univ., East Lansing, MI (United States)
- Washington State Univ., Pullman, WA (United States)
- Donald Danforth Plant Science Center, St. Louis, MO (United States); National Center of Biomedical Analysis, Beijing (China)
- New Mexico State Univ., Las Cruces, NM (United States)
- Cambridge Advanced Imaging Centre, Cambridge (United Kingdom)
- Donald Danforth Plant Science Center, St. Louis, MO (United States); Univ. of North Carolina, Chapel Hill, NC (United States)
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Center for Advanced Biofuel Systems (CABS); Donald Danforth Plant Science Center, St. Louis, MO (United States); Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1459199
- Alternate Identifier(s):
- OSTI ID: 1602547
- Grant/Contract Number:
- SC0001295; FG02-91ER20021
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Plant Physiology (Bethesda)
- Additional Journal Information:
- Journal Name: Plant Physiology (Bethesda); Journal Volume: 167; Journal Issue: 2; Journal ID: ISSN 0032-0889
- Publisher:
- American Society of Plant Biologists
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Juergens, Matthew T., Deshpande, Rahul R., Lucker, Ben F., Park, Jeong -Jin, Wang, Hongxia, Gargouri, Mahmoud, Holguin, F. Omar, Disbrow, Bradley, Schaub, Tanner, Skepper, Jeremy N., Kramer, David M., Gang, David R., Hicks, Leslie M., and Shachar-Hill, Yair. The Regulation of Photosynthetic Structure and Function during Nitrogen Deprivation in Chlamydomonas reinhardtii. United States: N. p., 2014.
Web. doi:10.1104/pp.114.250530.
Juergens, Matthew T., Deshpande, Rahul R., Lucker, Ben F., Park, Jeong -Jin, Wang, Hongxia, Gargouri, Mahmoud, Holguin, F. Omar, Disbrow, Bradley, Schaub, Tanner, Skepper, Jeremy N., Kramer, David M., Gang, David R., Hicks, Leslie M., & Shachar-Hill, Yair. The Regulation of Photosynthetic Structure and Function during Nitrogen Deprivation in Chlamydomonas reinhardtii. United States. https://doi.org/10.1104/pp.114.250530
Juergens, Matthew T., Deshpande, Rahul R., Lucker, Ben F., Park, Jeong -Jin, Wang, Hongxia, Gargouri, Mahmoud, Holguin, F. Omar, Disbrow, Bradley, Schaub, Tanner, Skepper, Jeremy N., Kramer, David M., Gang, David R., Hicks, Leslie M., and Shachar-Hill, Yair. Mon .
"The Regulation of Photosynthetic Structure and Function during Nitrogen Deprivation in Chlamydomonas reinhardtii". United States. https://doi.org/10.1104/pp.114.250530. https://www.osti.gov/servlets/purl/1459199.
@article{osti_1459199,
title = {The Regulation of Photosynthetic Structure and Function during Nitrogen Deprivation in Chlamydomonas reinhardtii},
author = {Juergens, Matthew T. and Deshpande, Rahul R. and Lucker, Ben F. and Park, Jeong -Jin and Wang, Hongxia and Gargouri, Mahmoud and Holguin, F. Omar and Disbrow, Bradley and Schaub, Tanner and Skepper, Jeremy N. and Kramer, David M. and Gang, David R. and Hicks, Leslie M. and Shachar-Hill, Yair},
abstractNote = {The accumulation of carbon storage compounds by many unicellular algae after nutrient deprivation occurs despite declines in their photosynthetic apparatus. To understand the regulation and roles of photosynthesis during this potentially bioenergetically valuable process, we analyzed photosynthetic structure and function after nitrogen deprivation in the model alga Chlamydomonas reinhardtii. Transcriptomic, proteomic, metabolite, and lipid profiling and microscopic time course data were combined with multiple measures of photosynthetic function. Levels of transcripts and proteins of photosystems I and II and most antenna genes fell with differing trajectories; thylakoid membrane lipid levels decreased, while their proportions remained similar and thylakoid membrane organization appeared to be preserved. Cellular chlorophyll (Chl) content decreased more than 2-fold within 24 h, and we conclude from transcript protein and 13C labeling rates that Chl synthesis was down-regulated both pre- and posttranslationally and that Chl levels fell because of a rapid cessation in synthesis and dilution by cellular growth rather than because of degradation. Photosynthetically driven oxygen production and the efficiency of photosystem II as well as P700+ reduction and electrochromic shift kinetics all decreased over the time course, without evidence of substantial energy overflow. The results also indicate that linear electron flow fell approximately 15% more than cyclic flow over the first 24 h. Comparing Calvin-Benson cycle transcript and enzyme levels with changes in photosynthetic 13CO2 incorporation rates also pointed to a coordinated multilevel down-regulation of photosynthetic fluxes during starch synthesis before the induction of high triacylglycerol accumulation rates.},
doi = {10.1104/pp.114.250530},
journal = {Plant Physiology (Bethesda)},
number = 2,
volume = 167,
place = {United States},
year = {Mon Dec 08 00:00:00 EST 2014},
month = {Mon Dec 08 00:00:00 EST 2014}
}
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