Characterizing DNA Corona Rigidity in DNA-Directed Gold Nanoparticle Crystalline Structures

Lee, Ha Youn; Ren, Casey; Park, Sung Yong

doi:10.1021/acs.jpcc.6b05396

Title: Characterizing DNA Corona Rigidity in DNA-Directed Gold Nanoparticle Crystalline Structures

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Abstract

DNA-linked gold nanoparticle systems have become an adaptable self-assembly tool for programming various crystalline orders. The programmability lies in the DNA corona, a highly dense outer shell of DNA linkers that binds the nanoparticles together. Various self-assembled structures can be formed by changing the properties of the DNA corona, particularly the size ratio. However, uncertainty still remains as to the actual behavior and structural rigidity of the DNA itself, impeding advances in structural diversification. Specifically, the DNA could adopt an A-form structure after crystallization, or a more flexible DNA corona could be generated if B-form DNA persists but becomes physically bent and adsorbed to the nanoparticle surface in crystal formations. To understand the rigidity of the DNA corona, we devised a method to stretch the DNA between two nanoparticles by simultaneously using short and long linkers. This linker combination flexibly acts as a single linker, rather than two separate linkers that create two ranges of interactions. By measuring the edge-to-edge distance between the two nanoparticles using both linkers in small-angle X-ray scattering (SAXS) experiments, we recorded the first per-base contribution that is consistent with the B-form DNA rise of 3.4 Å. Here, the elucidation of DNA behavior within nanoparticle crystalsmore »« less

Authors:

Lee, Ha Youn ^[1]; Ren, Casey ^[1]; Park, Sung Yong ^[1]

Univ. of Southern California, Los Angeles, CA (United States)

Publication Date:: Mon Jul 25 00:00:00 EDT 2016

Research Org.:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Org.:: USDOE Office of Science (SC); National Institutes of Health (NIH)

OSTI Identifier:: 1459023

Grant/Contract Number:: AC02-06CH11357; R01-AI083115

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Physical Chemistry. C

Additional Journal Information:: Journal Volume: 120; Journal Issue: 32; Journal ID: ISSN 1932-7447

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: ENGLISH

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; crystals; metal nanoparticles; genetics; nanoparticles; X-ray scattering

Citation Formats


                    Lee, Ha Youn, Ren, Casey, and Park, Sung Yong. Characterizing DNA Corona Rigidity in DNA-Directed Gold Nanoparticle Crystalline Structures.  United States: N. p., 2016. 
Web.  doi:10.1021/acs.jpcc.6b05396.

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                    Lee, Ha Youn, Ren, Casey, & Park, Sung Yong. Characterizing DNA Corona Rigidity in DNA-Directed Gold Nanoparticle Crystalline Structures.  United States.  https://doi.org/10.1021/acs.jpcc.6b05396

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                    Lee, Ha Youn, Ren, Casey, and Park, Sung Yong. Mon .  
"Characterizing DNA Corona Rigidity in DNA-Directed Gold Nanoparticle Crystalline Structures".  United States.  https://doi.org/10.1021/acs.jpcc.6b05396.  https://www.osti.gov/servlets/purl/1459023.

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@article{osti_1459023,

  title        = {Characterizing DNA Corona Rigidity in DNA-Directed Gold Nanoparticle Crystalline Structures},

  author       = {Lee, Ha Youn and Ren, Casey and Park, Sung Yong},

  abstractNote = {DNA-linked gold nanoparticle systems have become an adaptable self-assembly tool for programming various crystalline orders. The programmability lies in the DNA corona, a highly dense outer shell of DNA linkers that binds the nanoparticles together. Various self-assembled structures can be formed by changing the properties of the DNA corona, particularly the size ratio. However, uncertainty still remains as to the actual behavior and structural rigidity of the DNA itself, impeding advances in structural diversification. Specifically, the DNA could adopt an A-form structure after crystallization, or a more flexible DNA corona could be generated if B-form DNA persists but becomes physically bent and adsorbed to the nanoparticle surface in crystal formations. To understand the rigidity of the DNA corona, we devised a method to stretch the DNA between two nanoparticles by simultaneously using short and long linkers. This linker combination flexibly acts as a single linker, rather than two separate linkers that create two ranges of interactions. By measuring the edge-to-edge distance between the two nanoparticles using both linkers in small-angle X-ray scattering (SAXS) experiments, we recorded the first per-base contribution that is consistent with the B-form DNA rise of 3.4 Å. Here, the elucidation of DNA behavior within nanoparticle crystals is essential for understanding the programmability of DNA-linked nanoparticle systems.},

  doi          = {10.1021/acs.jpcc.6b05396},

  journal      = {Journal of Physical Chemistry. C},

  number       = 32,

  volume       = 120,

  place        = {United States},

  year         = {Mon Jul 25 00:00:00 EDT 2016},

  month        = {Mon Jul 25 00:00:00 EDT 2016}

}

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