Microbial community structure with trends in methylation gene diversity and abundance in mercury-contaminated rice paddy soils in Guizhou, China
Abstract
Paddy soils from mercury (Hg)-contaminated rice fields in Guizhou, China were studied with respect to total mercury (THg) and methylmercury (MeHg) concentrations as well as Bacterial and Archaeal community composition. Total Hg (0.25-990 μg g-1) and MeHg (1.3-30.5 ng g-1) varied between samples. Pyrosequencing (454 FLX) of the hypervariable v1-v3 regions of the 16S rRNA genes showed that Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria, Euryarchaeota, and Crenarchaeota were dominant in all samples. The Bacterial α-diversity was higher in samples with relatively Low THg and MeHg and decreased with increasing THg and MeHg concentrations. In contrast, Archaeal α-diversity increased with increasing of MeHg concentrations but did not correlate with changes in THg concentrations. Overall, the methylation gene hgcAB copy number increased with both increasing THg and MeHg concentrations. The microbial communities at High THg and High MeHg appear to be adapted by species that are both Hg resistant and carry hgcAB genes for MeHg production. The relatively high abundance of both sulfate-reducing δ-Proteobacteria and methanogenic Archaea, as well as their positive correlations with increasing THg and MeHg concentrations, suggests that these microorganisms are the primary Hg-methylators in the rice paddy soils in Guizhou, China.
- Authors:
-
[1];
[1];
[1];
[1];
[1];
[1]
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Chinese Academy of Science, Guiyang (China)
- Univ. of Oklahoma, Norman, OK (United States)
- Chinese Academy of Science, Guiyang (China)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1435339
- Alternate Identifier(s):
- OSTI ID: 1434080; OSTI ID: 1479398
- Grant/Contract Number:
- AC05-00OR22725; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science: Processes & Impacts
- Additional Journal Information:
- Journal Volume: 20; Journal Issue: 4; Journal ID: ISSN 2050-7887
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Mercury; methylmercury; Hg-methylation; hgcAB; rice paddy soil; pyrosequencing
Citation Formats
Vishnivetskaya, Tatiana A., Hu, Haiyan, Van Nostrand, Joy D., Wymore, Ann M., Xu, Xiaohang, Qiu, Guangle, Feng, Xinbin, Zhou, Jizhong, Brown, Steven D., Brandt, Craig C., Podar, Mircea, Gu, Baohua, and Elias, Dwayne A. Microbial community structure with trends in methylation gene diversity and abundance in mercury-contaminated rice paddy soils in Guizhou, China. United States: N. p., 2018.
Web. doi:10.1039/c7em00558j.
Vishnivetskaya, Tatiana A., Hu, Haiyan, Van Nostrand, Joy D., Wymore, Ann M., Xu, Xiaohang, Qiu, Guangle, Feng, Xinbin, Zhou, Jizhong, Brown, Steven D., Brandt, Craig C., Podar, Mircea, Gu, Baohua, & Elias, Dwayne A. Microbial community structure with trends in methylation gene diversity and abundance in mercury-contaminated rice paddy soils in Guizhou, China. United States. https://doi.org/10.1039/c7em00558j
Vishnivetskaya, Tatiana A., Hu, Haiyan, Van Nostrand, Joy D., Wymore, Ann M., Xu, Xiaohang, Qiu, Guangle, Feng, Xinbin, Zhou, Jizhong, Brown, Steven D., Brandt, Craig C., Podar, Mircea, Gu, Baohua, and Elias, Dwayne A. Mon .
"Microbial community structure with trends in methylation gene diversity and abundance in mercury-contaminated rice paddy soils in Guizhou, China". United States. https://doi.org/10.1039/c7em00558j. https://www.osti.gov/servlets/purl/1435339.
@article{osti_1435339,
title = {Microbial community structure with trends in methylation gene diversity and abundance in mercury-contaminated rice paddy soils in Guizhou, China},
author = {Vishnivetskaya, Tatiana A. and Hu, Haiyan and Van Nostrand, Joy D. and Wymore, Ann M. and Xu, Xiaohang and Qiu, Guangle and Feng, Xinbin and Zhou, Jizhong and Brown, Steven D. and Brandt, Craig C. and Podar, Mircea and Gu, Baohua and Elias, Dwayne A.},
abstractNote = {Paddy soils from mercury (Hg)-contaminated rice fields in Guizhou, China were studied with respect to total mercury (THg) and methylmercury (MeHg) concentrations as well as Bacterial and Archaeal community composition. Total Hg (0.25-990 μg g-1) and MeHg (1.3-30.5 ng g-1) varied between samples. Pyrosequencing (454 FLX) of the hypervariable v1-v3 regions of the 16S rRNA genes showed that Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria, Euryarchaeota, and Crenarchaeota were dominant in all samples. The Bacterial α-diversity was higher in samples with relatively Low THg and MeHg and decreased with increasing THg and MeHg concentrations. In contrast, Archaeal α-diversity increased with increasing of MeHg concentrations but did not correlate with changes in THg concentrations. Overall, the methylation gene hgcAB copy number increased with both increasing THg and MeHg concentrations. The microbial communities at High THg and High MeHg appear to be adapted by species that are both Hg resistant and carry hgcAB genes for MeHg production. The relatively high abundance of both sulfate-reducing δ-Proteobacteria and methanogenic Archaea, as well as their positive correlations with increasing THg and MeHg concentrations, suggests that these microorganisms are the primary Hg-methylators in the rice paddy soils in Guizhou, China.},
doi = {10.1039/c7em00558j},
journal = {Environmental Science: Processes & Impacts},
number = 4,
volume = 20,
place = {United States},
year = {Mon Mar 05 00:00:00 EST 2018},
month = {Mon Mar 05 00:00:00 EST 2018}
}
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