Title: Metabolic versatility of small archaea Micrarchaeota and Parvarchaeota

Abstract

Small acidophilic archaea belonging to Micrarchaeota and Parvarchaeota phyla are known to physically interact with some Thermoplasmatales members in nature. However, due to a lack of cultivation and limited genomes on hand, their biodiversity, metabolisms, and physiologies remain largely unresolved. For this study, we obtained 39 genomes from acid mine drainage (AMD) and hot spring environments around the world. 16S rRNA gene based analyses revealed that Parvarchaeota were only detected in AMD and hot spring habitats, while Micrarchaeota were also detected in others including soil, peat, hypersaline mat, and freshwater, suggesting a considerable higher diversity and broader than expected habitat distribution for this phylum. Despite their small genomes (0.64-1.08 Mb), these archaea may contribute to carbon and nitrogen cycling by degrading multiple saccharides and proteins, and produce ATP via aerobic respiration and fermentation. Additionally, we identified several syntenic genes with homology to those involved in iron oxidation in six Parvarchae ota genomes, suggesting their potential role in iron cycling. However, both phyla lack biosynthetic pathways for amino acids and nucleotides, suggesting that they likely scavenge these biomolecules from the environment and/or other community members. Moreover, low-oxygen enrichments in laboratory confirmed our speculation that both phyla are microaerobic/anaerobic, based on severalmore » specific genes identified in them. Furthermore, phylogenetic analyses provide insights into the close evolutionary history of energy related functionalities between both phyla with Thermoplasmatales. These results expand our understanding of these elusive archaea by revealing their involvement in carbon, nitrogen, and iron cycling, and suggest their potential interactions with Thermoplasmatales on genomic scale.« less

Authors:

Chen, Lin-Xing  ^[1];

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• Search DOE PAGES for ORCID "0000-0003-2774-1952"

Mendez-Garcia, Celia  ^[2];

• Search DOE PAGES for author "Mendez-Garcia, Celia"
• Search DOE PAGES for ORCID "0000-0002-8587-0114"

Dombrowski, Nina  ^[3];

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• Search DOE PAGES for ORCID "0000-0003-1917-2577"

Servin-Garciduenas, Luis E. ^[4]; Eloe-Fadrosh, Emiley A. ^[5]; Fang, Bao-Zhu ^[1]; Luo, Zhen-Hao ^[1]; Tan, Sha ^[1]; Zhi, Xiao-Yang ^[6]; Hua, Zheng-Shuang ^[1]; Martinez-Romero, Esperanza ^[7]; Woyke, Tanja ^[5]; Huang, Li-Nan ^[1]; Sanchez, Jesus ^[8]; Pelaez, Ana Isabel ^[8]; Ferrer, Manuel ^[9]; Baker, Brett J. ^[3]; Shu, Wen-Sheng ^[10]

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+ Show Author Affiliations

1. Sun Yat-Sen Univ., Guangzhou, (China). State Key Lab. of Biocontrol, Guangdong Key Lab. of Plant Resources and College of Ecology and Evolution
2. Univ. of Oviedo (Spain). Dept. of Functional Biology and Univ. Inst. of Biotechnology of Asturias (IUBA); Univ. of Illinois, Urbana, IL (United States). Carl R. Woese Inst. for Genomic Biology
3. Univ. of Texas, Austin, TX (United States). Dept. of Marine Science and Marine Science Inst.
4. National Autonomous Univ. of Mexico, Morelia (Mexico). Lab. of Microbiomics and National School of Higher Studies Morelia
5. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
6. Yunnan Univ., Kunming (China). Yunnan Inst. of Microbiology
7. National Autonomous Univ. of Mexico, Morelia (Mexico). Dept. of Ecological Genomics and Center for Genomic Sciences
8. Univ. of Oviedo (Spain). Dept. of Functional Biology and Univ. Inst. of Biotechnology of Asturias (IUBA)
9. Spanish National Research Council (CSIC), Madrid (Spain). Inst. of Catalysis
10. South China Normal Univ., Guangzhou (China). School of Life Sciences

Publication Date:

Fri Dec 08 00:00:00 EST 2017

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE Office of Science (SC); Univ. of California, Berkeley, CA (United States); National Center for Biotechnology Information (NCBI); China Univ. of Geosciences, Wuhan (China); Univ. of Massachusetts, Amherst, MA (United States); Univ. of Konstanz (Germany); National Natural Science Foundation of China (NSFC); Alfred P. Sloan Foundation; National Autonomous Univ. of Mexico, Morelia (Mexico)

OSTI Identifier:

1434028

Grant/Contract Number:  

AC02-05CH11231; 31600101; U1201233; 31570500; PAPIIT IA210617

Resource Type:

Accepted Manuscript

Journal Name:

The ISME Journal

Additional Journal Information:

Journal Volume: 12; Journal Issue: 3; Related Information: © 2017 The Author(s) 2017, under exclusive licence to Macmillan Publishers Limited, part of Springer Nature.; Journal ID: ISSN 1751-7362

Publisher:

Nature Publishing Group

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; Metagenomics; Water microbiology