HIV envelope V3 region mimic embodies key features of a broadly neutralizing antibody lineage epitope
Abstract
HIV-1 envelope (Env) mimetics are candidate components of prophylactic vaccines and potential therapeutics. Here we use a synthetic V3-glycopeptide (“Man9-V3”) for structural studies of an HIV Env third variable loop (V3)-glycan directed, broadly neutralizing antibody (bnAb) lineage (“DH270”), to visualize the epitope on Env and to study how affinity maturation of the lineage proceeded. Unlike many previous V3 mimetics, Man9-V3 encompasses two key features of the V3 region recognized by V3-glycan bnAbs—the conserved GDIR motif and the N332 glycan. In our structure of an antibody fragment of a lineage member, DH270.6, in complex with the V3 glycopeptide, the conformation of the antibody-bound glycopeptide conforms closely to that of the corresponding segment in an intact HIV-1 Env trimer. An additional structure identifies roles for two critical mutations in the development of breadth. The results suggest a strategy for use of a V3 glycopeptide as a vaccine immunogen.
- Authors:
-
[1];
[1];
- Harvard Medical School, Boston, MA (United States)
- Duke Univ. Medical Center, Durham, NC (United States); Duke Human Vaccine Inst., Durham, NC (United States)
- Duke Human Vaccine Inst., Durham, NC (United States); Duke Univ. Medical Center, Durham, NC (United States)
- Sloan Kettering Institute, New York, NY (United States)
- Swarthmore College, PA (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division; National Institutes of Health (NIH); NIH-ORIP HEI; National Inst. of Allergy and Infectious Diseases; Medical Scientist Training Program (MSTP)
- OSTI Identifier:
- 1431358
- Grant/Contract Number:
- AC02-06CH11357; P41 GM103403; S10 RR029205; AI100645; T32GM007171; Al127193
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Antibodies; HIV infections; Structural biology; Vaccines
Citation Formats
Fera, Daniela, Lee, Matthew S., Wiehe, Kevin, Meyerhoff, R. Ryan, Piai, Alessandro, Bonsignori, Mattia, Aussedat, Baptiste, Walkowicz, William E., Ton, Therese, Zhou, Jeffrey O., Danishefsky, Samuel, Haynes, Barton F., and Harrison, Stephen C. HIV envelope V3 region mimic embodies key features of a broadly neutralizing antibody lineage epitope. United States: N. p., 2018.
Web. doi:10.1038/s41467-018-03565-6.
Fera, Daniela, Lee, Matthew S., Wiehe, Kevin, Meyerhoff, R. Ryan, Piai, Alessandro, Bonsignori, Mattia, Aussedat, Baptiste, Walkowicz, William E., Ton, Therese, Zhou, Jeffrey O., Danishefsky, Samuel, Haynes, Barton F., & Harrison, Stephen C. HIV envelope V3 region mimic embodies key features of a broadly neutralizing antibody lineage epitope. United States. https://doi.org/10.1038/s41467-018-03565-6
Fera, Daniela, Lee, Matthew S., Wiehe, Kevin, Meyerhoff, R. Ryan, Piai, Alessandro, Bonsignori, Mattia, Aussedat, Baptiste, Walkowicz, William E., Ton, Therese, Zhou, Jeffrey O., Danishefsky, Samuel, Haynes, Barton F., and Harrison, Stephen C. Fri .
"HIV envelope V3 region mimic embodies key features of a broadly neutralizing antibody lineage epitope". United States. https://doi.org/10.1038/s41467-018-03565-6. https://www.osti.gov/servlets/purl/1431358.
@article{osti_1431358,
title = {HIV envelope V3 region mimic embodies key features of a broadly neutralizing antibody lineage epitope},
author = {Fera, Daniela and Lee, Matthew S. and Wiehe, Kevin and Meyerhoff, R. Ryan and Piai, Alessandro and Bonsignori, Mattia and Aussedat, Baptiste and Walkowicz, William E. and Ton, Therese and Zhou, Jeffrey O. and Danishefsky, Samuel and Haynes, Barton F. and Harrison, Stephen C.},
abstractNote = {HIV-1 envelope (Env) mimetics are candidate components of prophylactic vaccines and potential therapeutics. Here we use a synthetic V3-glycopeptide (“Man9-V3”) for structural studies of an HIV Env third variable loop (V3)-glycan directed, broadly neutralizing antibody (bnAb) lineage (“DH270”), to visualize the epitope on Env and to study how affinity maturation of the lineage proceeded. Unlike many previous V3 mimetics, Man9-V3 encompasses two key features of the V3 region recognized by V3-glycan bnAbs—the conserved GDIR motif and the N332 glycan. In our structure of an antibody fragment of a lineage member, DH270.6, in complex with the V3 glycopeptide, the conformation of the antibody-bound glycopeptide conforms closely to that of the corresponding segment in an intact HIV-1 Env trimer. An additional structure identifies roles for two critical mutations in the development of breadth. The results suggest a strategy for use of a V3 glycopeptide as a vaccine immunogen.},
doi = {10.1038/s41467-018-03565-6},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {Fri Mar 16 00:00:00 EDT 2018},
month = {Fri Mar 16 00:00:00 EDT 2018}
}
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Works referenced in this record:
Affinity Maturation of a Potent Family of HIV Antibodies Is Primarily Focused on Accommodating or Avoiding Glycans
journal, December 2015
- Garces, Fernando; Lee, Jeong Hyun; de Val, Natalia
- Immunity, Vol. 43, Issue 6
Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env
journal, June 2015
- Do Kwon, Young; Pancera, Marie; Acharya, Priyamvada
- Nature Structural & Molecular Biology, Vol. 22, Issue 7
Broadly neutralizing antibodies and the search for an HIV-1 vaccine: the end of the beginning
journal, August 2013
- Kwong, Peter D.; Mascola, John R.; Nabel, Gary J.
- Nature Reviews Immunology, Vol. 13, Issue 9
Solving structures of protein complexes by molecular replacement with Phaser
journal, December 2006
- McCoy, Airlie J.
- Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 1
Natively glycosylated HIV-1 Env structure reveals new mode for antibody recognition of the CD4-binding site
journal, September 2016
- Gristick, Harry B.; von Boehmer, Lotta; West Jr, Anthony P.
- Nature Structural & Molecular Biology, Vol. 23, Issue 10
Affinity maturation in an HIV broadly neutralizing B-cell lineage through reorientation of variable domains
journal, June 2014
- Fera, D.; Schmidt, A. G.; Haynes, B. F.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 28
Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design
journal, May 2016
- Burton, Dennis R.; Hangartner, Lars
- Annual Review of Immunology, Vol. 34, Issue 1
Staged induction of HIV-1 glycan–dependent broadly neutralizing antibodies
journal, March 2017
- Bonsignori, Mattia; Kreider, Edward F.; Fera, Daniela
- Science Translational Medicine, Vol. 9, Issue 381
An Advance in the Chemical Synthesis of Homogeneous N-Linked Glycopolypeptides by Convergent Aspartylation
journal, September 2012
- Wang, Ping; Aussedat, Baptiste; Vohra, Yusufbhai
- Angewandte Chemie International Edition, Vol. 51, Issue 46
Intraprotomer masking of third variable loop (V3) epitopes by the first and second variable loops (V1V2) within the native HIV-1 envelope glycoprotein trimer
journal, November 2011
- Liu, L.; Cimbro, R.; Lusso, P.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 50
Glycosylation in cancer: mechanisms and clinical implications
journal, August 2015
- Pinho, Salomé S.; Reis, Celso A.
- Nature Reviews Cancer, Vol. 15, Issue 9
MolProbity: all-atom contacts and structure validation for proteins and nucleic acids
journal, May 2007
- Davis, I. W.; Leaver-Fay, A.; Chen, V. B.
- Nucleic Acids Research, Vol. 35, Issue Web Server
Chemical Synthesis of Highly Congested gp120 V1V2 N -Glycopeptide Antigens for Potential HIV-1-Directed Vaccines
journal, August 2013
- Aussedat, Baptiste; Vohra, Yusuf; Park, Peter K.
- Journal of the American Chemical Society, Vol. 135, Issue 35
Structure-based design of a constrained peptide mimic of the HIV-1 V3 loop neutralization site 1 1 Edited by F.E. Cohen
journal, February 1997
- Ghiara, Jayant B.; Ferguson, David C.; Satterthwait, Arnold C.
- Journal of Molecular Biology, Vol. 266, Issue 1
Max Bergmann lecture Protein epitope mimetics in the age of structural vaccinology: PROTEIN EPITOPE MIMETICS
journal, January 2013
- Robinson, John A.
- Journal of Peptide Science, Vol. 19, Issue 3
Molecular switch for alternative conformations of the HIV-1 V3 region: Implications for phenotype conversion
journal, September 2006
- Rosen, O.; Sharon, M.; Quadt-Akabayov, S. R.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 38
XDS
journal, January 2010
- Kabsch, Wolfgang
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
Passenger transgenes reveal intrinsic specificity of the antibody hypermutation mechanism: clustering, polarity, and specific hot spots.
journal, March 1993
- Betz, A. G.; Rada, C.; Pannell, R.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 6
Early Antibody Lineage Diversification and Independent Limb Maturation Lead to Broad HIV-1 Neutralization Targeting the Env High-Mannose Patch
journal, May 2016
- MacLeod, Daniel T.; Choi, Nancy M.; Briney, Bryan
- Immunity, Vol. 44, Issue 5
Model Building and Refinement of a Natively Glycosylated HIV-1 Env Protein by High-Resolution Cryoelectron Microscopy
journal, October 2015
- Lee, Jeong Hyun; de Val, Natalia; Lyumkis, Dmitry
- Structure, Vol. 23, Issue 10
Analysis of the structural correlates for antibody polyreactivity by multiple reassortments of chimeric human immunoglobulin heavy and light chain V segments
journal, September 1994
- Ichiyoshi, Y.
- Journal of Experimental Medicine, Vol. 180, Issue 3
Multiple Antibody Lineages in One Donor Target the Glycan-V3 Supersite of the HIV-1 Envelope Glycoprotein and Display a Preference for Quaternary Binding
journal, September 2016
- Longo, Nancy S.; Sutton, Matthew S.; Shiakolas, Andrea R.
- Journal of Virology, Vol. 90, Issue 23
Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G
journal, May 2016
- Stewart-Jones, Guillaume B. E.; Soto, Cinque; Lemmin, Thomas
- Cell, Vol. 165, Issue 4
Interaction of the gp120 V1V2 loop with a neighboring gp120 unit shields the HIV envelope trimer against cross-neutralizing antibodies
journal, June 2011
- Rusert, Peter; Krarup, Anders; Magnus, Carsten
- The Journal of Experimental Medicine, Vol. 208, Issue 7
Structural Rationale for the Broad Neutralization of HIV-1 by Human Monoclonal Antibody 447-52D
journal, February 2004
- Stanfield, Robyn L.; Gorny, Miroslaw K.; Williams, Constance
- Structure, Vol. 12, Issue 2
Linear PADRE T Helper Epitope and Carbohydrate B Cell Epitope Conjugates Induce Specific High Titer IgG Antibody Responses
journal, February 2000
- Alexander, Jeff; del Guercio, Marie-France; Maewal, Ajesh
- The Journal of Immunology, Vol. 164, Issue 3
Global site-specific N-glycosylation analysis of HIV envelope glycoprotein
journal, March 2017
- Cao, Liwei; Diedrich, Jolene K.; Kulp, Daniel W.
- Nature Communications, Vol. 8, Issue 1
Models of Somatic Hypermutation Targeting and Substitution Based on Synonymous Mutations from High-Throughput Immunoglobulin Sequencing Data
journal, January 2013
- Yaari, Gur; Vander Heiden, Jason A.; Uduman, Mohamed
- Frontiers in Immunology, Vol. 4
Structural principles controlling HIV envelope glycosylation
journal, June 2017
- Behrens, Anna-Janina; Crispin, Max
- Current Opinion in Structural Biology, Vol. 44
PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
Reconstructing a B-Cell Clonal Lineage. II. Mutation, Selection, and Affinity Maturation
journal, April 2014
- Kepler, Thomas B.; Munshaw, Supriya; Wiehe, Kevin
- Frontiers in Immunology, Vol. 5
Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody
journal, May 2016
- Kong, R.; Xu, K.; Zhou, T.
- Science, Vol. 352, Issue 6287
A Potent and Broad Neutralizing Antibody Recognizes and Penetrates the HIV Glycan Shield
journal, October 2011
- Pejchal, R.; Doores, K. J.; Walker, L. M.
- Science, Vol. 334, Issue 6059
Structural Evolution of Glycan Recognition by a Family of Potent HIV Antibodies
journal, September 2014
- Garces, Fernando; Sok, Devin; Kong, Leopold
- Cell, Vol. 159, Issue 1
NMRPipe: A multidimensional spectral processing system based on UNIX pipes
journal, November 1995
- Delaglio, Frank; Grzesiek, Stephan; Vuister, GeertenW.
- Journal of Biomolecular NMR, Vol. 6, Issue 3
B-cell–lineage immunogen design in vaccine development with HIV-1 as a case study
journal, May 2012
- Haynes, Barton F.; Kelsoe, Garnett; Harrison, Stephen C.
- Nature Biotechnology, Vol. 30, Issue 5
A Broadly Neutralizing Antibody Targets the Dynamic HIV Envelope Trimer Apex via a Long, Rigidified, and Anionic β-Hairpin Structure
journal, April 2017
- Lee, Jeong Hyun; Andrabi, Raiees; Su, Ching-Yao
- Immunity, Vol. 46, Issue 4
Reconstructing a B-cell clonal lineage. I. Statistical inference of unobserved ancestors
journal, January 2013
- Kepler, Thomas B.
- F1000Research, Vol. 2
Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gp120
journal, May 2013
- Kong, Leopold; Lee, Jeong Hyun; Doores, Katie J.
- Nature Structural & Molecular Biology, Vol. 20, Issue 7
Conserved structural elements in the V3 crown of HIV-1 gp120
journal, July 2010
- Jiang, Xunqing; Burke, Valicia; Totrov, Maxim
- Nature Structural & Molecular Biology, Vol. 17, Issue 8
A Prominent Site of Antibody Vulnerability on HIV Envelope Incorporates a Motif Associated with CCR5 Binding and Its Camouflaging Glycans
journal, July 2016
- Sok, Devin; Pauthner, Matthias; Briney, Bryan
- Immunity, Vol. 45, Issue 1
HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies
journal, September 2016
- Steichen, Jon M.; Kulp, Daniel W.; Tokatlian, Talar
- Immunity, Vol. 45, Issue 3
Molecular structure of eight human autoreactive monoclonal antibodies
journal, March 2001
- Aguilera, I.; Melero, J.; Nunez-Roldan, A.
- Immunology, Vol. 102, Issue 3
Structural Basis of the Cross-Reactivity of Genetically Related Human Anti-HIV-1 mAbs: Implications for Design of V3-Based Immunogens
journal, November 2009
- Burke, Valicia; Williams, Constance; Sukumaran, Madhav
- Structure, Vol. 17, Issue 11
Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide
journal, March 2017
- Alam, S. Munir; Aussedat, Baptiste; Vohra, Yusuf
- Science Translational Medicine, Vol. 9, Issue 381
Glycosylation: Heterogeneity and the 3D Structure of Proteins
journal, January 1997
- Rudd, Pauline M.; Dwek, Raymond A.
- Critical Reviews in Biochemistry and Molecular Biology, Vol. 32, Issue 1
An Advance in the Chemical Synthesis of Homogeneous N-Linked Glycopolypeptides by Convergent Aspartylation
journal, September 2012
- Wang, Ping; Aussedat, Baptiste; Vohra, Yusufbhai
- Angewandte Chemie, Vol. 124, Issue 46
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation
journal, February 2021
- Tillu, Vikas A.; Rae, James; Gao, Ya
- Nature Communications, Vol. 12, Issue 1
Multi-functionality of a tryptophan residue conserved in substrate-binding groove of GH19 chitinases
journal, January 2021
- Nagata, Takuya; Shinya, Shoko; Ohnuma, Takayuki
- Scientific Reports, Vol. 11, Issue 1
Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide
text, January 2017
- Hua-Xin, Liao,; R., Mascola, John; Amanda, Eaton,
- The University of North Carolina at Chapel Hill University Libraries
Interaction of the gp120 V1V2 loop with a neighboring gp120 unit shields the HIV envelope trimer against cross-neutralizing antibodies
text, January 2011
- Rusert, P.; Krarup, A.; Magnus, C.
- Rockefeller University Press
Staged induction of HIV-1 glycan–dependent broadly neutralizing antibodies
text, January 2017
- Todd, Bradley,; Baptiste, Aussedat,; Amit, Kumar,
- The University of North Carolina at Chapel Hill University Libraries
MolProbity: all-atom contacts and structure validation for proteins and nucleic acids
text, January 2007
- B., Chen, Vincent; C., Richardson, David; S., Richardson, Jane
- The University of North Carolina at Chapel Hill University Libraries
Max Bergmann lecture protein epitope mimetics in the age of structural vaccinology
text, January 2013
- Robinson, John A.
- Wiley-Blackwell
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Apollo - University of Cambridge Repository
An Advance in the Chemical Synthesis of Homogeneous N-Linked Glycopolypeptides by Convergent Aspartylation
journal, September 2012
- Wang, Ping; Aussedat, Baptiste; Vohra, Yusufbhai
- Angewandte Chemie International Edition, Vol. 51, Issue 46
Structural Evolution of Glycan Recognition by a Family of Potent HIV Antibodies
journal, September 2014
- Garces, Fernando; Sok, Devin; Kong, Leopold
- Cell, Vol. 159, Issue 1
Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G
journal, May 2016
- Stewart-Jones, Guillaume B. E.; Soto, Cinque; Lemmin, Thomas
- Cell, Vol. 165, Issue 4
Affinity Maturation of a Potent Family of HIV Antibodies Is Primarily Focused on Accommodating or Avoiding Glycans
journal, December 2015
- Garces, Fernando; Lee, Jeong Hyun; de Val, Natalia
- Immunity, Vol. 43, Issue 6
A Prominent Site of Antibody Vulnerability on HIV Envelope Incorporates a Motif Associated with CCR5 Binding and Its Camouflaging Glycans
journal, July 2016
- Sok, Devin; Pauthner, Matthias; Briney, Bryan
- Immunity, Vol. 45, Issue 1
Structural Rationale for the Broad Neutralization of HIV-1 by Human Monoclonal Antibody 447-52D
journal, February 2004
- Stanfield, Robyn L.; Gorny, Miroslaw K.; Williams, Constance
- Structure, Vol. 12, Issue 2
Chemical Synthesis of Highly Congested gp120 V1V2 N -Glycopeptide Antigens for Potential HIV-1-Directed Vaccines
journal, August 2013
- Aussedat, Baptiste; Vohra, Yusuf; Park, Peter K.
- Journal of the American Chemical Society, Vol. 135, Issue 35
Glycosylation in cancer: mechanisms and clinical implications
journal, August 2015
- Pinho, Salomé S.; Reis, Celso A.
- Nature Reviews Cancer, Vol. 15, Issue 9
Natively glycosylated HIV-1 Env structure reveals new mode for antibody recognition of the CD4-binding site
journal, September 2016
- Gristick, Harry B.; von Boehmer, Lotta; West Jr, Anthony P.
- Nature Structural & Molecular Biology, Vol. 23, Issue 10
Author Correction: Structure of the yeast Swi/Snf complex in a nucleosome free state
journal, November 2020
- Wang, Chengcheng; Guo, Zhouyan; Zhan, Xiechao
- Nature Communications, Vol. 11, Issue 1
Intraprotomer masking of third variable loop (V3) epitopes by the first and second variable loops (V1V2) within the native HIV-1 envelope glycoprotein trimer
journal, November 2011
- Liu, L.; Cimbro, R.; Lusso, P.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 50
Affinity maturation in an HIV broadly neutralizing B-cell lineage through reorientation of variable domains
journal, June 2014
- Fera, D.; Schmidt, A. G.; Haynes, B. F.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 28
Biochemical Analysis of Hypermutational Targeting by Wild Type and Mutant Activation-induced Cytidine Deaminase
journal, September 2004
- Bransteitter, Ronda; Pham, Phuong; Calabrese, Peter
- Journal of Biological Chemistry, Vol. 279, Issue 49
Analysis of the structural correlates for antibody polyreactivity by multiple reassortments of chimeric human immunoglobulin heavy and light chain V segments
journal, September 1994
- Ichiyoshi, Y.
- Journal of Experimental Medicine, Vol. 180, Issue 3
Coot model-building tools for molecular graphics
journal, November 2004
- Emsley, Paul; Cowtan, Kevin
- Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody
journal, May 2016
- Kong, R.; Xu, K.; Zhou, T.
- Science, Vol. 352, Issue 6287
Crystal Structures of Human Immunodeficiency Virus Type 1 (HIV-1) Neutralizing Antibody 2219 in Complex with Three Different V3 Peptides Reveal a New Binding Mode for HIV-1 Cross-Reactivity
journal, May 2006
- Stanfield, R. L.; Gorny, M. K.; Zolla-Pazner, S.
- Journal of Virology, Vol. 80, Issue 12
Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design
journal, May 2016
- Burton, Dennis R.; Hangartner, Lars
- Annual Review of Immunology, Vol. 34, Issue 1
Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide
text, January 2017
- Hua-Xin, Liao,; R., Mascola, John; Amanda, Eaton,
- The University of North Carolina at Chapel Hill University Libraries
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Apollo - University of Cambridge Repository
Glycosylation: Heterogeneity and the 3D Structure of Proteins
journal, January 1997
- Rudd, Pauline M.; Dwek, Raymond A.
- Critical Reviews in Biochemistry and Molecular Biology, Vol. 32, Issue 1
Models of Somatic Hypermutation Targeting and Substitution Based on Synonymous Mutations from High-Throughput Immunoglobulin Sequencing Data
journal, January 2013
- Yaari, Gur; Vander Heiden, Jason A.; Uduman, Mohamed
- Frontiers in Immunology, Vol. 4
Reconstructing a B-Cell Clonal Lineage. II. Mutation, Selection, and Affinity Maturation
journal, April 2014
- Kepler, Thomas B.; Munshaw, Supriya; Wiehe, Kevin
- Frontiers in Immunology, Vol. 5
Linear PADRE T Helper Epitope and Carbohydrate B Cell Epitope Conjugates Induce Specific High Titer IgG Antibody Responses
journal, February 2000
- Alexander, Jeff; del Guercio, Marie-France; Maewal, Ajesh
- The Journal of Immunology, Vol. 164, Issue 3
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journal, December 2019
- Saunders, Kevin O.; Wiehe, Kevin; Tian, Ming
- Science, Vol. 366, Issue 6470
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journal, September 2018
- Landais, Elise; Moore, Penny L.
- Retrovirology, Vol. 15, Issue 1
Glycopeptides and -Mimetics to Detect, Monitor and Inhibit Bacterial and Viral Infections: Recent Advances and Perspectives
journal, March 2019
- Behren, Sandra; Westerlind, Ulrika
- Molecules, Vol. 24, Issue 6
The Impact of Sustained Immunization Regimens on the Antibody Response to Oligomannose Glycans
journal, February 2020
- Nguyen, Dung N.; Redman, Richard L.; Horiya, Satoru
- ACS Chemical Biology, Vol. 15, Issue 3
Maternal Broadly Neutralizing Antibodies Can Select for Neutralization-Resistant, Infant-Transmitted/Founder HIV Variants
journal, April 2020
- Martinez, David R.; Tu, Joshua J.; Kumar, Amit
- mBio, Vol. 11, Issue 2
The Effects of Framework Mutations at the Variable Domain Interface on Antibody Affinity Maturation in an HIV-1 Broadly Neutralizing Antibody Lineage
journal, July 2020
- Zhou, Jeffrey O.; Zaidi, Hussain A.; Ton, Therese
- Frontiers in Immunology, Vol. 11
Glycopeptides and -Mimetics to Detect, Monitor and Inhibit Bacterial and Viral Infections: Recent Advances and Perspectives
journal, March 2019
- Behren, Sandra; Westerlind, Ulrika
- Molecules, Vol. 24, Issue 6