SENSITIVE TO FREEZING2 Aids in Resilience to Salt and Drought in Freezing-Sensitive Tomato

Wang, Kun; Hersh, Hope Lynn; Benning, Christoph

doi:10.1104/pp.16.01183

Title: SENSITIVE TO FREEZING2 Aids in Resilience to Salt and Drought in Freezing-Sensitive Tomato

Abstract

SENSITIVE TO FREEZING2 (SFR2) is crucial for protecting chloroplast membranes following freezing in Arabidopsis (Arabidopsis thaliana). It has been shown that SFR2 homologs are present in all land plants, including freezing-sensitive species, raising the question of SFR2 function beyond freezing tolerance. Similar to freezing, salt and drought can cause dehydration. Thus, it is hypothesized that in freezing-sensitive plants SFR2 may play roles in their resilience to salt or drought. To test this hypothesis, SlSFR2 RNAi lines were generated in the cold/freezing-sensitive species tomato (Solanum lycopersicum [M82 cv]). Hypersensitivity to salt and drought of SlSFR2-RNAi lines was observed. Higher tolerance of wild-type tomatoes was correlated with the production of trigalactosyldiacylglycerol, a product of SFR2 activity. Tomato SFR2 in vitro activity is Mg²⁺-dependent and its optimal pH is 7.5, similar to that of Arabidopsis SFR2, but the specific activity of tomato SFR2 in vitro is almost double that of Arabidopsis SFR2. When salt and drought stress were applied to Arabidopsis, no conditions could be identified at which SFR2 was induced prior to irreversibly impacting plant growth, suggesting that SFR2 protects Arabidopsis primarily against freezing. Discovery of tomato SFR2 function in drought and salt resilience provides further insights into general membrane lipid remodeling-basedmore »« less

Authors:

^[1]; Hersh, Hope Lynn ^[1];

^[1]

Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry and Molecular Biology and Dept. of Plant Biology; Michigan State Univ., East Lansing, MI (United States). MSU-Dept. of Energy Plant Research Lab.

Publication Date:: Tue Sep 06 00:00:00 EDT 2016

Research Org.:: Michigan State Univ., East Lansing, MI (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1425622

Grant/Contract Number:: FG02-98ER20305

Resource Type:: Accepted Manuscript

Journal Name:: Plant Physiology (Bethesda)

Additional Journal Information:: Journal Name: Plant Physiology (Bethesda); Journal Volume: 172; Journal Issue: 3; Journal ID: ISSN 0032-0889

Publisher:: American Society of Plant Biologists

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES

Citation Formats


                    Wang, Kun, Hersh, Hope Lynn, and Benning, Christoph. SENSITIVE TO FREEZING2 Aids in Resilience to Salt and Drought in Freezing-Sensitive Tomato.  United States: N. p., 2016. 
Web.  doi:10.1104/pp.16.01183.

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                    Wang, Kun, Hersh, Hope Lynn, & Benning, Christoph. SENSITIVE TO FREEZING2 Aids in Resilience to Salt and Drought in Freezing-Sensitive Tomato.  United States.  https://doi.org/10.1104/pp.16.01183

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                    Wang, Kun, Hersh, Hope Lynn, and Benning, Christoph. Tue .  
"SENSITIVE TO FREEZING2 Aids in Resilience to Salt and Drought in Freezing-Sensitive Tomato".  United States.  https://doi.org/10.1104/pp.16.01183.  https://www.osti.gov/servlets/purl/1425622.

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@article{osti_1425622,

  title        = {SENSITIVE TO FREEZING2 Aids in Resilience to Salt and Drought in Freezing-Sensitive Tomato},

  author       = {Wang, Kun and Hersh, Hope Lynn and Benning, Christoph},

  abstractNote = {SENSITIVE TO FREEZING2 (SFR2) is crucial for protecting chloroplast membranes following freezing in Arabidopsis (Arabidopsis thaliana). It has been shown that SFR2 homologs are present in all land plants, including freezing-sensitive species, raising the question of SFR2 function beyond freezing tolerance. Similar to freezing, salt and drought can cause dehydration. Thus, it is hypothesized that in freezing-sensitive plants SFR2 may play roles in their resilience to salt or drought. To test this hypothesis, SlSFR2 RNAi lines were generated in the cold/freezing-sensitive species tomato (Solanum lycopersicum [M82 cv]). Hypersensitivity to salt and drought of SlSFR2-RNAi lines was observed. Higher tolerance of wild-type tomatoes was correlated with the production of trigalactosyldiacylglycerol, a product of SFR2 activity. Tomato SFR2 in vitro activity is Mg2+-dependent and its optimal pH is 7.5, similar to that of Arabidopsis SFR2, but the specific activity of tomato SFR2 in vitro is almost double that of Arabidopsis SFR2. When salt and drought stress were applied to Arabidopsis, no conditions could be identified at which SFR2 was induced prior to irreversibly impacting plant growth, suggesting that SFR2 protects Arabidopsis primarily against freezing. Discovery of tomato SFR2 function in drought and salt resilience provides further insights into general membrane lipid remodeling-based stress tolerance mechanisms and together with protection against freezing in freezing-resistant plants such as Arabidopsis, it adds lipid remodeling as a possible target for the engineering of abiotic stress-resilient crops.},

  doi          = {10.1104/pp.16.01183},

  journal      = {Plant Physiology (Bethesda)},

  number       = 3,

  volume       = 172,

  place        = {United States},

  year         = {Tue Sep 06 00:00:00 EDT 2016},

  month        = {Tue Sep 06 00:00:00 EDT 2016}

}

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Differential Chloroplast Proteomics of Temperature Adaptation in Apple (Malus x domestica Borkh.) Microshoots
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An Arabidopsis protoplast isolation method reduces cytosolic acidification and activation of the chloroplast stress sensor SENSITIVE TO FREEZING 2
journal, June 2019

Barnes, Allison C.; Elowsky, Christian G.; Roston, Rebecca L.
Plant Signaling & Behavior, Vol. 14, Issue 9
DOI: 10.1080/15592324.2019.1629270

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Title: SENSITIVE TO FREEZING2 Aids in Resilience to Salt and Drought in Freezing-Sensitive Tomato

Abstract

Citation Formats

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