Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus
Abstract
Cortical microtubules (MTs) are evolutionarily conserved cytoskeletal components with specialized roles in plants, including regulation of cell wall biogenesis. MT functions and dynamics are dictated by the composition of their monomeric subunits, α- (TUA) and β-tubulins (TUB), which in animals and protists are subject to both transcriptional regulation and post-translational modifications (PTM). While spatiotemporal regulation of tubulin gene expression has been reported in plants, whether and to what extent tubulin PTMs occur in these species remain poorly understood. We chose the woody perennial Populus for investigation of tubulin PTMs in this study, with a particular focus on developing xylem where high tubulin transcript levels support MT-dependent secondary cell wall deposition. Mass spectrometry and immunodetection concurred that detyrosination, non-tyrosination and glutamylation were essentially absent in tubulins isolated from wood-forming tissues of P. deltoides and P. tremula ×alba. Label-free quantification of tubulin isotypes and RNA-Seq estimation of tubulin transcript abundance were largely consistent with transcriptional regulation. However, two TUB isotypes were detected at noticeably lower levels than expected based on RNA-Seq transcript abundance in both Populus species. These findings led us to conclude that MT composition during wood formation depends exclusively on transcriptional and, to a lesser extent, translational regulation of tubulinmore »
- Authors:
-
- Univ. of Georgia, Athens, GA (United States)
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1423767
- Grant/Contract Number:
- SC0008470
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES
Citation Formats
Hu, Hao, Gu, Xi, Xue, Liang-Jiao, Swamy, Prashant S., Harding, Scott A., and Tsai, Chung-Jui. Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus. United States: N. p., 2016.
Web. doi:10.3389/fpls.2016.01493.
Hu, Hao, Gu, Xi, Xue, Liang-Jiao, Swamy, Prashant S., Harding, Scott A., & Tsai, Chung-Jui. Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus. United States. https://doi.org/10.3389/fpls.2016.01493
Hu, Hao, Gu, Xi, Xue, Liang-Jiao, Swamy, Prashant S., Harding, Scott A., and Tsai, Chung-Jui. Thu .
"Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus". United States. https://doi.org/10.3389/fpls.2016.01493. https://www.osti.gov/servlets/purl/1423767.
@article{osti_1423767,
title = {Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus},
author = {Hu, Hao and Gu, Xi and Xue, Liang-Jiao and Swamy, Prashant S. and Harding, Scott A. and Tsai, Chung-Jui},
abstractNote = {Cortical microtubules (MTs) are evolutionarily conserved cytoskeletal components with specialized roles in plants, including regulation of cell wall biogenesis. MT functions and dynamics are dictated by the composition of their monomeric subunits, α- (TUA) and β-tubulins (TUB), which in animals and protists are subject to both transcriptional regulation and post-translational modifications (PTM). While spatiotemporal regulation of tubulin gene expression has been reported in plants, whether and to what extent tubulin PTMs occur in these species remain poorly understood. We chose the woody perennial Populus for investigation of tubulin PTMs in this study, with a particular focus on developing xylem where high tubulin transcript levels support MT-dependent secondary cell wall deposition. Mass spectrometry and immunodetection concurred that detyrosination, non-tyrosination and glutamylation were essentially absent in tubulins isolated from wood-forming tissues of P. deltoides and P. tremula ×alba. Label-free quantification of tubulin isotypes and RNA-Seq estimation of tubulin transcript abundance were largely consistent with transcriptional regulation. However, two TUB isotypes were detected at noticeably lower levels than expected based on RNA-Seq transcript abundance in both Populus species. These findings led us to conclude that MT composition during wood formation depends exclusively on transcriptional and, to a lesser extent, translational regulation of tubulin isotypes.},
doi = {10.3389/fpls.2016.01493},
journal = {Frontiers in Plant Science},
number = ,
volume = 7,
place = {United States},
year = {Thu Oct 13 00:00:00 EDT 2016},
month = {Thu Oct 13 00:00:00 EDT 2016}
}
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Figures / Tables:
FIGURE 1: Tubulin purification. (A) SDS-PAGE analysis of xylem protein extracts from various steps of the tubulin purification process. M, markers; CP, crude proteins; FT, flow-through; W, washed, unbound proteins; EP, eluted proteins enriched with tubulins. (B) A two-color Western blot of purified tubulins from P. deltoides (Pd) and P.more » x alba (Pta) normal wood (NW) or tension wood (TW) xylem probed with anti-TUA (red channel) or anti-TUB (green channel) antibodies. The scanning intensities were adjusted in the upper panel (reduced for red and increased for green) in order to visualize both TUA and TUB signals clearly. The lower panel was scanned at the same intensity for both channels.
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