The complex physiology of Cellvibrio japonicus xylan degradation relies on a single cytoplasmic β-xylosidase for xylo-oligosaccharide utilization
Abstract
Summary Lignocellulose degradation by microbes plays a central role in global carbon cycling, human gut metabolism and renewable energy technologies. While considerable effort has been put into understanding the biochemical aspects of lignocellulose degradation, much less work has been done to understand how these enzymes work in an in vivo context. Here, we report a systems level study of xylan degradation in the saprophytic bacterium Cellvibrio japonicus . Transcriptome analysis indicated seven genes that encode carbohydrate active enzymes were up‐regulated during growth with xylan containing media. In‐frame deletion analysis of these genes found that only gly43F is critical for utilization of xylo‐oligosaccharides, xylan, and arabinoxylan. Heterologous expression of gly43F was sufficient for the utilization of xylo‐oligosaccharides in Escherichia coli . Additional analysis found that the xyn11A , xyn11B , abf43L , abf43K , and abf51A gene products were critical for utilization of arabinoxylan. Furthermore, a predicted transporter (CJA_1315) was required for effective utilization of xylan substrates, and we propose this unannotated gene be called xntA (xylan transporter A). Our major findings are (i) C. japonicus employs both secreted and surface associated enzymes for xylan degradation, which differs from the strategy used for cellulose degradation, and (ii) a single cytoplasmic β‐xylosidasemore »
- Authors:
-
[1]
- Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States). Dept. of Biological Sciences
- Publication Date:
- Research Org.:
- Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- OSTI Identifier:
- 1415270
- Alternate Identifier(s):
- OSTI ID: 1416401
- Grant/Contract Number:
- SC0014183; DE‐SC0014183
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Molecular Microbiology
- Additional Journal Information:
- Journal Volume: 107; Journal Issue: 5; Journal ID: ISSN 0950-382X
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; β-xylosidase; Cellvibrio japonicus; polysaccharide degradation; xylan; xylanase
Citation Formats
Blake, Andrew D., Beri, Nina R., Guttman, Hadassa S., Cheng, Raymond, and Gardner, Jeffrey G. The complex physiology of Cellvibrio japonicus xylan degradation relies on a single cytoplasmic β-xylosidase for xylo-oligosaccharide utilization. United States: N. p., 2017.
Web. doi:10.1111/mmi.13903.
Blake, Andrew D., Beri, Nina R., Guttman, Hadassa S., Cheng, Raymond, & Gardner, Jeffrey G. The complex physiology of Cellvibrio japonicus xylan degradation relies on a single cytoplasmic β-xylosidase for xylo-oligosaccharide utilization. United States. https://doi.org/10.1111/mmi.13903
Blake, Andrew D., Beri, Nina R., Guttman, Hadassa S., Cheng, Raymond, and Gardner, Jeffrey G. Thu .
"The complex physiology of Cellvibrio japonicus xylan degradation relies on a single cytoplasmic β-xylosidase for xylo-oligosaccharide utilization". United States. https://doi.org/10.1111/mmi.13903. https://www.osti.gov/servlets/purl/1415270.
@article{osti_1415270,
title = {The complex physiology of Cellvibrio japonicus xylan degradation relies on a single cytoplasmic β-xylosidase for xylo-oligosaccharide utilization},
author = {Blake, Andrew D. and Beri, Nina R. and Guttman, Hadassa S. and Cheng, Raymond and Gardner, Jeffrey G.},
abstractNote = {Summary Lignocellulose degradation by microbes plays a central role in global carbon cycling, human gut metabolism and renewable energy technologies. While considerable effort has been put into understanding the biochemical aspects of lignocellulose degradation, much less work has been done to understand how these enzymes work in an in vivo context. Here, we report a systems level study of xylan degradation in the saprophytic bacterium Cellvibrio japonicus . Transcriptome analysis indicated seven genes that encode carbohydrate active enzymes were up‐regulated during growth with xylan containing media. In‐frame deletion analysis of these genes found that only gly43F is critical for utilization of xylo‐oligosaccharides, xylan, and arabinoxylan. Heterologous expression of gly43F was sufficient for the utilization of xylo‐oligosaccharides in Escherichia coli . Additional analysis found that the xyn11A , xyn11B , abf43L , abf43K , and abf51A gene products were critical for utilization of arabinoxylan. Furthermore, a predicted transporter (CJA_1315) was required for effective utilization of xylan substrates, and we propose this unannotated gene be called xntA (xylan transporter A). Our major findings are (i) C. japonicus employs both secreted and surface associated enzymes for xylan degradation, which differs from the strategy used for cellulose degradation, and (ii) a single cytoplasmic β‐xylosidase is essential for the utilization of xylo‐oligosaccharides.},
doi = {10.1111/mmi.13903},
journal = {Molecular Microbiology},
number = 5,
volume = 107,
place = {United States},
year = {Thu Dec 21 00:00:00 EST 2017},
month = {Thu Dec 21 00:00:00 EST 2017}
}
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Cited by: 17 works
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Figures / Tables:
Fig. 1. C. japonicus CAZyme genes up-regulated during exponential growth using xylan.: C. japonicus CAZyme genes up-regulated during exponential growth using xylan. The volcano plot summarizes RNAseq comparative analysis of gene expression for xylan grown cells versus glucose grown cells. Fold change (log2 scale) is on the x-axis, with positivemore »
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