Herpes simplex virus 1 induces egress channels through marginalized host chromatin
Abstract
Lytic infection with herpes simplex virus type 1 (HSV-1) induces profound modification of the cell nucleus including formation of a viral replication compartment and chromatin marginalization into the nuclear periphery. Here, we used three-dimensional soft X-ray tomography, combined with cryogenic fluorescence, confocal and electron microscopy, to analyse the transformation of peripheral chromatin during HSV-1 infection. Our data showed an increased presence of low-density gaps in the marginalized chromatin at late infection. Advanced data analysis indicated the formation of virus-nucleocapsid-sized (or wider) channels extending through the compacted chromatin of the host. Importantly, confocal and electron microscopy analysis showed that these gaps frequently contained viral nucleocapsids. Our results demonstrated that HSV-1 infection induces the formation of channels penetrating the compacted layer of cellular chromatin and allowing for the passage of progeny viruses to the nuclear envelope, their site of nuclear egress.
- Authors:
-
- Univ. of Jyvaskyla (Finland). Dept. of Physics and Nanoscience Center
- Univ. of Jyvaskyla (Finland). Dept. of Biological and Environmental Science and Nanoscience Center
- Univ. of California, San Francisco, CA (United States). Dept. of Anatomy; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Univ. of Turku (Finland)
- International Center for Research in Infectiology, Lyon (France); Ecole Normale Superieure, Lyon (France); Univ. of Lyon (France). LabEx Ecofect
- Univ. of Jyvaskyla (Finland). Dept. of Physics and Nanoscience Center; ITMO uUniv., St. Petersburg (Russia)
- Univ. of California, San Francisco, CA (United States). Dept. of Anatomy; Univ. of Turku (Finland)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1414761
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; herpes virus; nuclear organization
Citation Formats
Myllys, Markko, Ruokolainen, Visa, Aho, Vesa, Smith, Elizabeth A., Hakanen, Satu, Peri, Piritta, Salvetti, Anna, Timonen, Jussi, Hukkanen, Veijo, Larabell, Carolyn A., and Vihinen-Ranta, Maija. Herpes simplex virus 1 induces egress channels through marginalized host chromatin. United States: N. p., 2016.
Web. doi:10.1038/srep28844.
Myllys, Markko, Ruokolainen, Visa, Aho, Vesa, Smith, Elizabeth A., Hakanen, Satu, Peri, Piritta, Salvetti, Anna, Timonen, Jussi, Hukkanen, Veijo, Larabell, Carolyn A., & Vihinen-Ranta, Maija. Herpes simplex virus 1 induces egress channels through marginalized host chromatin. United States. https://doi.org/10.1038/srep28844
Myllys, Markko, Ruokolainen, Visa, Aho, Vesa, Smith, Elizabeth A., Hakanen, Satu, Peri, Piritta, Salvetti, Anna, Timonen, Jussi, Hukkanen, Veijo, Larabell, Carolyn A., and Vihinen-Ranta, Maija. Tue .
"Herpes simplex virus 1 induces egress channels through marginalized host chromatin". United States. https://doi.org/10.1038/srep28844. https://www.osti.gov/servlets/purl/1414761.
@article{osti_1414761,
title = {Herpes simplex virus 1 induces egress channels through marginalized host chromatin},
author = {Myllys, Markko and Ruokolainen, Visa and Aho, Vesa and Smith, Elizabeth A. and Hakanen, Satu and Peri, Piritta and Salvetti, Anna and Timonen, Jussi and Hukkanen, Veijo and Larabell, Carolyn A. and Vihinen-Ranta, Maija},
abstractNote = {Lytic infection with herpes simplex virus type 1 (HSV-1) induces profound modification of the cell nucleus including formation of a viral replication compartment and chromatin marginalization into the nuclear periphery. Here, we used three-dimensional soft X-ray tomography, combined with cryogenic fluorescence, confocal and electron microscopy, to analyse the transformation of peripheral chromatin during HSV-1 infection. Our data showed an increased presence of low-density gaps in the marginalized chromatin at late infection. Advanced data analysis indicated the formation of virus-nucleocapsid-sized (or wider) channels extending through the compacted chromatin of the host. Importantly, confocal and electron microscopy analysis showed that these gaps frequently contained viral nucleocapsids. Our results demonstrated that HSV-1 infection induces the formation of channels penetrating the compacted layer of cellular chromatin and allowing for the passage of progeny viruses to the nuclear envelope, their site of nuclear egress.},
doi = {10.1038/srep28844},
journal = {Scientific Reports},
number = 1,
volume = 6,
place = {United States},
year = {Tue Jun 28 00:00:00 EDT 2016},
month = {Tue Jun 28 00:00:00 EDT 2016}
}
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Works referencing / citing this record:
Chromatin organization regulates viral egress dynamics
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- Aho, Vesa; Myllys, Markko; Ruokolainen, Visa
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Venture from the Interior—Herpesvirus pUL31 Escorts Capsids from Nucleoplasmic Replication Compartments to Sites of Primary Envelopment at the Inner Nuclear Membrane
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