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Title: Simultaneously Synchrotron X-ray Fluorescence and Ptychographic Imaging of Frozen Biological Single Cells

Abstract

Bionanoprobe (BNP), a hard x-ray fluorescence sample-scanning nanoprobe at the Advanced Photon Source of Argonne National Laboratory, has been used to quantitatively study elemental distributions in biological cells with sub-100 nm spatial resolution and high sensitivity. Cryogenic conditions enable biological samples to be studied in their frozen-hydrated state with both ultrastructure and elemental distributions more faithfully preserved compared to conventional chemical fixation or dehydration methods. Furthermore, radiation damage is reduced in two ways: the diffusion rate of free radicals is decreased at low temperatures; and the sample is embedded in vitrified ice, which reduces mass loss.

Authors:
 [1];  [2];  [1];  [2];  [3];  [1];  [4];  [5];  [1]
+ Show Author Affiliations
  1. Argonne National Lab. (ANL), Argonne, IL (United States)
  2. Northwestern Univ., Evanston, IL (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  4. Northwestern Univ., Argonne, IL (United States)
  5. Argonne National Lab. (ANL), Argonne, IL (United States); Northwestern Univ., Evanston, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1411022
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Microscopy and Microanalysis
Additional Journal Information:
Journal Volume: 22; Journal Issue: S3; Journal ID: ISSN 1431-9276
Publisher:
Microscopy Society of America (MSA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Chen, S., Deng, J., Nashed, Y. S. G., Jin, Q., Vine, D. J., Peterka, T., Brister, K., Jacobsen, C., and Vogt, S. Simultaneously Synchrotron X-ray Fluorescence and Ptychographic Imaging of Frozen Biological Single Cells. United States: N. p., 2016. Web. doi:10.1017/S1431927616001306.
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Chen, S., Deng, J., Nashed, Y. S. G., Jin, Q., Vine, D. J., Peterka, T., Brister, K., Jacobsen, C., & Vogt, S. Simultaneously Synchrotron X-ray Fluorescence and Ptychographic Imaging of Frozen Biological Single Cells. United States. https://doi.org/10.1017/S1431927616001306
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Chen, S., Deng, J., Nashed, Y. S. G., Jin, Q., Vine, D. J., Peterka, T., Brister, K., Jacobsen, C., and Vogt, S. Mon . "Simultaneously Synchrotron X-ray Fluorescence and Ptychographic Imaging of Frozen Biological Single Cells". United States. https://doi.org/10.1017/S1431927616001306. https://www.osti.gov/servlets/purl/1411022.
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@article{osti_1411022,
title = {Simultaneously Synchrotron X-ray Fluorescence and Ptychographic Imaging of Frozen Biological Single Cells},
author = {Chen, S. and Deng, J. and Nashed, Y. S. G. and Jin, Q. and Vine, D. J. and Peterka, T. and Brister, K. and Jacobsen, C. and Vogt, S.},
abstractNote = {Bionanoprobe (BNP), a hard x-ray fluorescence sample-scanning nanoprobe at the Advanced Photon Source of Argonne National Laboratory, has been used to quantitatively study elemental distributions in biological cells with sub-100 nm spatial resolution and high sensitivity. Cryogenic conditions enable biological samples to be studied in their frozen-hydrated state with both ultrastructure and elemental distributions more faithfully preserved compared to conventional chemical fixation or dehydration methods. Furthermore, radiation damage is reduced in two ways: the diffusion rate of free radicals is decreased at low temperatures; and the sample is embedded in vitrified ice, which reduces mass loss.},
doi = {10.1017/S1431927616001306},
journal = {Microscopy and Microanalysis},
number = S3,
volume = 22,
place = {United States},
year = {Mon Jul 25 00:00:00 EDT 2016},
month = {Mon Jul 25 00:00:00 EDT 2016}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1017/S1431927616001306
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Works referenced in this record:

Zernike phase contrast in scanning microscopy with X-rays
journal, September 2010

  • Holzner, Christian; Feser, Michael; Vogt, Stefan
  • Nature Physics, Vol. 6, Issue 11
  • DOI: 10.1038/nphys1765

Continuous motion scan ptychography: characterization for increased speed in coherent x-ray imaging
journal, January 2015

  • Deng, Junjing; Nashed, Youssef S. G.; Chen, Si
  • Optics Express, Vol. 23, Issue 5
  • DOI: 10.1364/OE.23.005438

Reconstructing state mixtures from diffraction measurements
journal, February 2013

The Bionanoprobe: hard X-ray fluorescence nanoprobe with cryogenic capabilities
journal, December 2013

Radiation processes in frozen aqueous systems
journal, January 1982

Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae
journal, February 2015

  • Deng, Junjing; Vine, David J.; Chen, Si
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 8
  • DOI: 10.1073/pnas.1413003112

Atomic radiative and radiationless yields for K and L shells
journal, April 1979

  • Krause, M. O.
  • Journal of Physical and Chemical Reference Data, Vol. 8, Issue 2
  • DOI: 10.1063/1.555594
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