Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Enamel ribbons, surface nodules, and octacalcium phosphate in C57BL/6 Amelx-/- mice and Amelx+/- lyonization

Abstract

Amelogenin is required for normal enamel formation and is the most abundant protein in developing enamel. Methods. Amelx+/+, Amelx+/- and Amelx-/- molars and incisors from C57BL/6 mice were characterized using RT-PCR, Western blotting, dissecting and light microscopy, immunohistochemistry (IHC), transmission electron microscopy (TEM), scanning electron microscopy (SEM), backscattered SEM (bSEM), nanohardness testing, and X-ray diffraction. No amelogenin protein was detected by Western blot analyses of enamel extracts from Amelx-/- mice. Amelx-/- incisor enamel averaged 20.3±3.3 μm in thickness, or only 1/6th that of the wild-type (122.3±7.9 μm). Amelx-/- incisor enamel nanohardness was 1.6 Gpa, less than half that of wild-type enamel (3.6 Gpa). Amelx+/- incisors and molars showed vertical banding patterns unique to each tooth. IHC detected no amelogenin in Amelx-/- enamel and varied levels of amelogenin in Amelx+/- incisors, which correlated positively with enamel thickness, strongly supporting lyonization as the cause of the variations in enamel thickness. TEM analyses showed characteristic mineral ribbons in Amelx+/+ and Amelx-/- enamel extending from mineralized dentin collagen to the ameloblast. The Amelx-/- enamel ribbons were not well-separated by matrix and appeared to fuse together, forming plates. Furthermore, x-ray diffraction determined that the predominant mineral in Amelx-/- enamel is octacalcium phosphate (not calcium hydroxyapatite).more » Amelx-/- ameloblasts were similar to wild-type ameloblasts except no Tomes’ processes extended into the thin enamel. Amelx-/- and Amelx+/- molars both showed calcified nodules on their occlusal surfaces. Histology of D5 and D11 developing molars showed nodules forming during the maturation stage. Amelogenin forms a resorbable matrix that separates and supports, but does not shape early secretory stage enamel ribbons. Amelogenin may facilitate the conversion of enamel ribbons into hydroxyapatite by inhibiting the formation of octacalcium phosphate. Finally, amelogenin is necessary for thickening the enamel layer, which helps maintain ribbon organization and development and maintenance of the Tomes process.« less

Authors:
 [1];  [2];  [3];  [1];  [4];  [1];  [1]
+ Show Author Affiliations
  1. Univ. of Michigan School of Dentistry, Ann Arbor, MI (United States). Dept. of Biologic and Materials Sciences
  2. Univ. of Michigan School of Dentistry, Ann Arbor, MI (United States). Dept. of Biologic and Materials Sciences; McGill Univ., Montreal, QC (Canada). Dept. of Anatomy and Cell Biology
  3. Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source
  4. Univ. of Michigan School of Dentistry, Ann Arbor, MI (United States). Dept. of Biologic and Materials Sciences; Peking Univ., Beijing (China). School of Stomatology
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH)
OSTI Identifier:
1395543
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Molecular Genetics & Genomic Medicine
Additional Journal Information:
Journal Volume: 4; Journal Issue: 6; Journal ID: ISSN 2324-9269
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Amelogenesis imperfecta; octacalcium phosphate; amelogenin; ameloblast; molar; incisor.

Citation Formats

Hu, Yuanyuan, Smith, Charles E., Cai, Zhonghou, Donnelly, Lorenza A. -J., Yang, Jie, Hu, Jan C. -C., and Simmer, James P. Enamel ribbons, surface nodules, and octacalcium phosphate in C57BL/6 Amelx-/- mice and Amelx+/- lyonization. United States: N. p., 2016. Web. doi:10.1002/mgg3.252.
Copy to clipboard
Hu, Yuanyuan, Smith, Charles E., Cai, Zhonghou, Donnelly, Lorenza A. -J., Yang, Jie, Hu, Jan C. -C., & Simmer, James P. Enamel ribbons, surface nodules, and octacalcium phosphate in C57BL/6 Amelx-/- mice and Amelx+/- lyonization. United States. https://doi.org/10.1002/mgg3.252
Copy to clipboard
Hu, Yuanyuan, Smith, Charles E., Cai, Zhonghou, Donnelly, Lorenza A. -J., Yang, Jie, Hu, Jan C. -C., and Simmer, James P. Wed . "Enamel ribbons, surface nodules, and octacalcium phosphate in C57BL/6 Amelx-/- mice and Amelx+/- lyonization". United States. https://doi.org/10.1002/mgg3.252. https://www.osti.gov/servlets/purl/1395543.
Copy to clipboard
@article{osti_1395543,
title = {Enamel ribbons, surface nodules, and octacalcium phosphate in C57BL/6 Amelx-/- mice and Amelx+/- lyonization},
author = {Hu, Yuanyuan and Smith, Charles E. and Cai, Zhonghou and Donnelly, Lorenza A. -J. and Yang, Jie and Hu, Jan C. -C. and Simmer, James P.},
abstractNote = {Amelogenin is required for normal enamel formation and is the most abundant protein in developing enamel. Methods. Amelx+/+, Amelx+/- and Amelx-/- molars and incisors from C57BL/6 mice were characterized using RT-PCR, Western blotting, dissecting and light microscopy, immunohistochemistry (IHC), transmission electron microscopy (TEM), scanning electron microscopy (SEM), backscattered SEM (bSEM), nanohardness testing, and X-ray diffraction. No amelogenin protein was detected by Western blot analyses of enamel extracts from Amelx-/- mice. Amelx-/- incisor enamel averaged 20.3±3.3 μm in thickness, or only 1/6th that of the wild-type (122.3±7.9 μm). Amelx-/- incisor enamel nanohardness was 1.6 Gpa, less than half that of wild-type enamel (3.6 Gpa). Amelx+/- incisors and molars showed vertical banding patterns unique to each tooth. IHC detected no amelogenin in Amelx-/- enamel and varied levels of amelogenin in Amelx+/- incisors, which correlated positively with enamel thickness, strongly supporting lyonization as the cause of the variations in enamel thickness. TEM analyses showed characteristic mineral ribbons in Amelx+/+ and Amelx-/- enamel extending from mineralized dentin collagen to the ameloblast. The Amelx-/- enamel ribbons were not well-separated by matrix and appeared to fuse together, forming plates. Furthermore, x-ray diffraction determined that the predominant mineral in Amelx-/- enamel is octacalcium phosphate (not calcium hydroxyapatite). Amelx-/- ameloblasts were similar to wild-type ameloblasts except no Tomes’ processes extended into the thin enamel. Amelx-/- and Amelx+/- molars both showed calcified nodules on their occlusal surfaces. Histology of D5 and D11 developing molars showed nodules forming during the maturation stage. Amelogenin forms a resorbable matrix that separates and supports, but does not shape early secretory stage enamel ribbons. Amelogenin may facilitate the conversion of enamel ribbons into hydroxyapatite by inhibiting the formation of octacalcium phosphate. Finally, amelogenin is necessary for thickening the enamel layer, which helps maintain ribbon organization and development and maintenance of the Tomes process.},
doi = {10.1002/mgg3.252},
journal = {Molecular Genetics & Genomic Medicine},
number = 6,
volume = 4,
place = {United States},
year = {Wed Oct 05 00:00:00 EDT 2016},
month = {Wed Oct 05 00:00:00 EDT 2016}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1002/mgg3.252
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 28 works
Citation information provided by
Web of Science
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

An amelogenin gene defect associated with human X-linked amelogenesis imperfecta
journal, March 1997

Detection of a Novel Mutation in X-linked Amelogenesis Imperfecta
journal, December 2000

Earliest enamel deposits of the rat incisor examined by electron microscopy, electron diffraction, and electron probe microanalysis
journal, March 1988

  • Landis, William J.; Burke, Grace Y.; Neuringer, Julia R.
  • The Anatomical Record, Vol. 220, Issue 3
  • DOI: 10.1002/ar.1092200303

Enamel proteins and proteases in Mmp20 and Klk4 null and double-null mice: Mmp20/Klk4 double-null mice
journal, December 2011

Molecular Cloning of Bovine Amelogenin cDna
journal, December 1987

Complete amino acid sequence of amelogenin in developing bovine enamel
journal, June 1984

  • Takagi, Tohru; Suzuki, Michiko; Baba, Tomomi
  • Biochemical and Biophysical Research Communications, Vol. 121, Issue 2
  • DOI: 10.1016/0006-291x(84)90223-7

DNA sequence for cloned cDNA for murine amelogenin reveal the amino acid sequence for enamel-specific protein
journal, June 1985

  • Snead, Malcolm L.; Lau, Eduardo C.; Zeichner-David, Margarita
  • Biochemical and Biophysical Research Communications, Vol. 129, Issue 3
  • DOI: 10.1016/0006-291x(85)91964-3

A new frameshift mutation encoding a truncated amelogenin leads to X-linked amelogenesis imperfecta
journal, March 2002

Genetic basis for the evolution of vertebrate mineralized tissue
journal, July 2004

  • Kawasaki, K.; Suzuki, T.; Weiss, K. M.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 31
  • DOI: 10.1073/pnas.0404279101

Effect of Kallikrein 4 Loss on Enamel Mineralization: COMPARISON WITH MICE LACKING MATRIX METALLOPROTEINASE 20
journal, March 2011

  • Smith, Charles E.; Richardson, Amelia S.; Hu, Yuanyuan
  • Journal of Biological Chemistry, Vol. 286, Issue 20
  • DOI: 10.1074/jbc.M110.194258

Ganoine Formation in the Scales of Primitive Actinopterygian Fishes, Lepisosteids and Polypterids
journal, January 1995

Cells from Hertwig's epithelial root sheath do not transcribe amelogenin
journal, January 1991

Dynamics of Enamel Formation in the Rat Incisor Tooth
journal, February 1979

Alteration of Conserved Alternative Splicing in AMELX Causes Enamel Defects
journal, August 2014

Characterisation of molecular defects in X-linked amelogenesis imperfecta (AIH1)
journal, January 1995

A simplified genetic design for mammalian enamel
journal, April 2011

Amelotin: an enamel matrix protein that experienced distinct evolutionary histories in amphibians, sauropsids and mammals
journal, March 2015

  • Gasse, Barbara; Chiari, Ylenia; Silvent, Jérémie
  • BMC Evolutionary Biology, Vol. 15, Issue 1
  • DOI: 10.1186/s12862-015-0329-x

Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1
journal, November 2007

  • Zou, YanMing; Wang, HongJun; Shapiro, Jason L.
  • Biochemical Journal, Vol. 408, Issue 3
  • DOI: 10.1042/BJ20070881

Cellular uptake of amelogenin, and its localization to CD63, and Lamp1-positive vesicles
journal, December 2006

  • Shapiro, J. L.; Wen, X.; Okamoto, C. T.
  • Cellular and Molecular Life Sciences, Vol. 64, Issue 2
  • DOI: 10.1007/s00018-006-6429-4

Transient amorphous calcium phosphate in forming enamel
journal, May 2009

  • Beniash, Elia; Metzler, Rebecca A.; Lam, Raymond S. K.
  • Journal of Structural Biology, Vol. 166, Issue 2
  • DOI: 10.1016/j.jsb.2009.02.001

Immunohistochemical demonstration of amelogenin penetration toward the dental pulp in the early stages of ameloblast development in rat molar tooth germs
journal, February 1991

  • Inai, Tetsuichiro; Kukita, Toshio; Ohsaki, Yasuyoshi
  • The Anatomical Record, Vol. 229, Issue 2
  • DOI: 10.1002/ar.1092290213

Cloning, cDNA Sequence, and Alternative Splicing of Porcine Amelogenin mRNAs
journal, October 1996

Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts
journal, December 2004

  • Fukumoto, Satoshi; Kiba, Takayoshi; Hall, Bradford
  • The Journal of Cell Biology, Vol. 167, Issue 5
  • DOI: 10.1083/jcb.200409077

Tooth Enamel Defects in Mice with a Deletion at the Arhgap6/AmelX Locus
journal, July 2005

  • Prakash, S. K.; Gibson, C. W.; Wright, J. T.
  • Calcified Tissue International, Vol. 77, Issue 1
  • DOI: 10.1007/s00223-004-1213-7

Resolution of ray-finned fish phylogeny and timing of diversification
journal, August 2012

  • Near, T. J.; Eytan, R. I.; Dornburg, A.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 34
  • DOI: 10.1073/pnas.1206625109

Characterization of Recombinant Pig Enamelysin Activity and Cleavage of Recombinant Pig and Mouse Amelogenins
journal, March 1999

Analysis of amelogenin mRNA during bovine tooth development
journal, February 1996

A novel mutation in the AMELX gene and multiple crown resorptions: AMELX mutation and multiple crown resorptions
journal, December 2011

Mammalian X-Chromosome Inactivation
journal, December 1983

Unique Enamel Phenotype Associated with Amelogenin Gene (AMELX) Codon 41 Point Mutation
journal, July 2000

Enamel Defects and Ameloblast-specific Expression in Enam Knock-out /lacZ Knock-in Mice
journal, February 2008

  • Hu, Jan C. -C.; Hu, Yuanyuan; Smith, Charles E.
  • Journal of Biological Chemistry, Vol. 283, Issue 16
  • DOI: 10.1074/jbc.M710565200

Amelogenesis Imperfecta: Genotype-Phenotype Studies in 71 Families
journal, January 2011

  • Wright, J. Timothy; Torain, Melody; Long, Kimberly
  • Cells Tissues Organs, Vol. 194, Issue 2-4
  • DOI: 10.1159/000324339

Spatial- and temporal-restricted pattern for amelogenin gene expression during mouse molar tooth organogenesis
journal, September 1988

  • Snead, Malcolm L.; Luo, Wen; Lau, Eduardo C.
  • Development, Vol. 104, Issue 1
  • DOI: 10.1242/dev.104.1.77

Alternative Splicing of the Amelogenin Gene in a Caudate Amphibian, Plethodon cinereus
journal, June 2013

ITGB6 loss-of-function mutations cause autosomal recessive amelogenesis imperfecta
journal, December 2013

  • Wang, Shih-Kai; Choi, Murim; Richardson, Amelia S.
  • Human Molecular Genetics, Vol. 23, Issue 8
  • DOI: 10.1093/hmg/ddt611

Mouse Genetic Background Influences the Dental Phenotype
journal, January 2013

  • Li, Yong; Konicki, William S.; Wright, J. Timothy
  • Cells Tissues Organs, Vol. 198, Issue 6
  • DOI: 10.1159/000360157

Mouse Genetic Background Influences the Dental Phenotype
journal, January 2013

  • Li, Yong; Konicki, William S.; Wright, J. Timothy
  • Cells Tissues Organs, Vol. 198, Issue 6
  • DOI: 10.1159/000360157

Correlated physicochemical and age changes in embryonic bovine enamel
journal, December 1983

  • Landis, William J.; Navarro, Maria
  • Calcified Tissue International, Vol. 35, Issue 1
  • DOI: 10.1007/bf02405006

Amelogenin p.M1T and p.W4S Mutations Underlying Hypoplastic X-linked Amelogenesis Imperfecta
journal, May 2004

Molecular Phylogeny and Divergence Times of Deuterostome Animals
journal, July 2005

  • Blair, Jaime E.; Hedges, S. Blair
  • Molecular Biology and Evolution, Vol. 22, Issue 11
  • DOI: 10.1093/molbev/msi225

Growth of calcium phosphates on hydroxyapatite crystals: The effect of magnesium
journal, December 1975

A large interstitial deletion encompassing the amelogenin gene on the short arm of the Y chromosome
journal, February 2005

  • Lattanzi, Wanda; Di Giacomo, Marilena C.; Lenato, Gennaro M.
  • Human Genetics, Vol. 116, Issue 5
  • DOI: 10.1007/s00439-004-1238-z

ITGB6 loss-of-function mutations cause autosomal recessive amelogenesis imperfecta
journal, December 2013

  • Wang, Shih-Kai; Choi, Murim; Richardson, Amelia S.
  • Human Molecular Genetics, Vol. 23, Issue 8
  • DOI: 10.1093/hmg/ddt611

Amelogenesis Imperfecta in Two Families with Defined AMELX Deletions in ARHGAP6
journal, December 2012

A Novel Missense Mutation (p.P52R) in Amelogenin Gene Causing X-linked Amelogenesis Imperfecta
journal, January 2007

Amelogenesis imperfecta phenotype–genotype correlations with two amelogenin gene mutations
journal, April 2002

New genomic and fossil data illuminate the origin of enamel
journal, September 2015

  • Qu, Qingming; Haitina, Tatjana; Zhu, Min
  • Nature, Vol. 526, Issue 7571
  • DOI: 10.1038/nature15259

Proteolytic activity of opossum tooth extracts
journal, January 1998

Human and mouse amelogenin gene loci are on the sex chromosomes
journal, February 1989

Gene expression and immunolocalisation of amelogenins in developing embryonic and neonatal hamster teeth
journal, May 1997

  • Karg, H. A.; Burger, E. H.; Lyaruu, D. M.
  • Cell and Tissue Research, Vol. 288, Issue 3
  • DOI: 10.1007/s004410050840

Origin, Splicing, and Expression of Rodent Amelogenin Exon 8
journal, October 2006

Evidence for a single loss of mineralized teeth in the common avian ancestor
journal, December 2014

Characterization of putative secretory sites on ameloblasts of the rat incisor
journal, October 1984

Amelogenin post-secretory processing during biomineralization in the postnatal mouse molar tooth
journal, January 1991

The Development of Enamel Structure
journal, September 1967

Partial expression of sex-linked recessive amelogenesis imperfecta in females compatible with the Lyon hypothesis
journal, February 1967

Critical roles for WDR72 in calcium transport and matrix protein removal during enamel maturation
journal, March 2015

  • Wang, Shih-Kai; Hu, Yuanyuan; Yang, Jie
  • Molecular Genetics & Genomic Medicine, Vol. 3, Issue 4
  • DOI: 10.1002/mgg3.143

Studies on the ultrastructure of dental enamel—II
journal, January 1962

Amelogenin-deficient Mice Display an Amelogenesis Imperfecta Phenotype
text, January 2001

  • An, Yuan, Zhi; Bradford, Hall,; B., Kulkarni, Ashok
  • The University of North Carolina at Chapel Hill University Libraries
  • DOI: 10.17615/nqc3-rk36

Single Bovine Enamel Particles Examined by Electron Optics
journal, May 1984

A deletion in the amelogenin gene (AMG) causes X-linked amelogenesis imperfecta (AIH1)
journal, August 1991

Gene Action in the X-chromosome of the Mouse (Mus musculus L.)
journal, April 1961

Target gene analyses of 39 amelogenesis imperfecta kindreds: AI mutations
journal, December 2011

Evolutionary Story of Mammalian-specific Amelogenin Exons 4, “4b”, 8, and 9
journal, September 2011

Characterisation of molecular defects in X-linked amelogenesis imperfecta (AIH1)
journal, January 1995

A thermodynamic analysis of the amorphous to crystalline calcium phosphate transformation
journal, December 1978

  • Meyer, J. L.; Eanes, E. D.
  • Calcified Tissue Research, Vol. 25, Issue 1
  • DOI: 10.1007/bf02010752

A Jurassic eutherian mammal and divergence of marsupials and placentals
journal, August 2011

  • Luo, Zhe-Xi; Yuan, Chong-Xi; Meng, Qing-Jin
  • Nature, Vol. 476, Issue 7361
  • DOI: 10.1038/nature10291

Analysis of amelogenin mRNA during bovine tooth development
journal, February 1996

The amelogenin story: origin and evolution
journal, May 2006

Effects of phosphorylation on the self-assembly of native full-length porcine amelogenin and its regulation of calcium phosphate formation in vitro
journal, February 2011

  • Wiedemann-Bidlack, Felicitas B.; Kwak, Seo-Young; Beniash, Elia
  • Journal of Structural Biology, Vol. 173, Issue 2
  • DOI: 10.1016/j.jsb.2010.11.006

Maturation ameloblasts of the porcine tooth germ do not express amelogenin
journal, March 1999

  • Wakida, K.; Amizuka, Norio; Murakami, Chikage
  • Histochemistry and Cell Biology, Vol. 111, Issue 4
  • DOI: 10.1007/s004180050360

Cloning, cDNA Sequence, and Alternative Splicing of Porcine Amelogenin mRNAs
journal, October 1996

Detection of a Novel Mutation in X-linked Amelogenesis Imperfecta
journal, December 2000

The growth of calcium phosphates on natural enamel
journal, December 1975

  • Tomazic, B.; Tomson, M.; Nancollas, G. H.
  • Calcified Tissue Research, Vol. 19, Issue 1
  • DOI: 10.1007/bf02564009

Cell proliferation and apoptosis in enamelin null mice: Enam null mice
journal, December 2011

Alternative splicing of the mouse amelogenin primary RNA transcript contributes to amelogenin heterogeneity
journal, November 1992

  • Lau, Eduardo C.; Simmer, James P.; Bringas, Pablo
  • Biochemical and Biophysical Research Communications, Vol. 188, Issue 3
  • DOI: 10.1016/0006-291X(92)91366-X

SSCP detection of a nonsense mutation in exon 5 of the amelogenin gene (AMGX) causing X-linked amelogenesis imperfecta (AIH1)
journal, January 1994

  • Lench, N. J.; Brook, A. H.; Winter, G. B.
  • Human Molecular Genetics, Vol. 3, Issue 5
  • DOI: 10.1093/hmg/3.5.827

Identification of a nonsense mutation in the amelogenin gene (AMELX) in a family with X-linked amelogenesis imperfecta (AIH1)
journal, December 1992

  • Aldred, MichaelJ.; Crawford, PeterJ. M.; Roberts, Enriqueta
  • Human Genetics, Vol. 90, Issue 4
  • DOI: 10.1007/bf00220469

Maturation ameloblasts of the porcine tooth germ do not express amelogenin
journal, March 1999

  • Wakida, K.; Amizuka, Norio; Murakami, Chikage
  • Histochemistry and Cell Biology, Vol. 111, Issue 4
  • DOI: 10.1007/s004180050360

A simplified genetic design for mammalian enamel
journal, April 2011

Amelotin: an enamel matrix protein that experienced distinct evolutionary histories in amphibians, sauropsids and mammals
journal, March 2015

  • Gasse, Barbara; Chiari, Ylenia; Silvent, Jérémie
  • BMC Evolutionary Biology, Vol. 15, Issue 1
  • DOI: 10.1186/s12862-015-0329-x

Identification of two additional exons at the 3′ end of the amelogenin gene
journal, June 1998

The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons
journal, March 2016

  • Braasch, Ingo; Gehrke, Andrew R.; Smith, Jeramiah J.
  • Nature Genetics, Vol. 48, Issue 4
  • DOI: 10.1038/ng.3526

Amelogenin-deficient Mice Display an Amelogenesis Imperfecta Phenotype
journal, June 2001

  • Gibson, Carolyn W.; Yuan, Zhi-An; Hall, Bradford
  • Journal of Biological Chemistry, Vol. 276, Issue 34
  • DOI: 10.1074/jbc.M104624200

Molecular Mechanisms of Dental Enamel Formation
journal, April 1995

Expression of amelogenin mRNA sequences during development of rat molars
journal, January 1996

  • Wurtz, Tilmann; Lundmark, Carin; Christersson, Cecilia
  • Journal of Bone and Mineral Research, Vol. 11, Issue 1
  • DOI: 10.1002/jbmr.5650110117

Unique Enamel Phenotype Associated with Amelogenin Gene (AMELX) Codon 41 Point Mutation
journal, July 2000

Alternative splicing of the mouse amelogenin primary RNA transcript
journal, October 1994

  • Simmer, J. P.; Hu, C. C.; Lau, E. C.
  • Calcified Tissue International, Vol. 55, Issue 4
  • DOI: 10.1007/BF00310410

Dynamics of Enamel Formation in the Rat Incisor Tooth
journal, February 1979

Cells from Hertwig's epithelial root sheath do not transcribe amelogenin
journal, January 1991

Molecular Cloning of Bovine Amelogenin cDna
journal, December 1987

Role of 20-kDa Amelogenin (P148) Phosphorylation in Calcium Phosphate Formation in Vitro
journal, May 2009

  • Kwak, Seo-Young; Wiedemann-Bidlack, Felicitas B.; Beniash, Elia
  • Journal of Biological Chemistry, Vol. 284, Issue 28
  • DOI: 10.1074/jbc.m109.020370

Tooth Enamel Defects in Mice with a Deletion at the Arhgap6/AmelX Locus
journal, July 2005

  • Prakash, S. K.; Gibson, C. W.; Wright, J. T.
  • Calcified Tissue International, Vol. 77, Issue 1
  • DOI: 10.1007/s00223-004-1213-7

Identification of the leucine-rich amelogenin peptide (LRAP) as the translation product of an alternatively spliced transcript
journal, February 1991

  • Gibson, Carolyn W.; Golub, Ellis; Ding, Wendi
  • Biochemical and Biophysical Research Communications, Vol. 174, Issue 3
  • DOI: 10.1016/0006-291x(91)91564-s

Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts
journal, December 2004

  • Fukumoto, Satoshi; Kiba, Takayoshi; Hall, Bradford
  • The Journal of Cell Biology, Vol. 167, Issue 5
  • DOI: 10.1083/jcb.200409077

SCPP genes in the coelacanth: Tissue mineralization genes shared by sarcopterygians: SCPP MINERALIZATION GENES IN THE COELACANTH
journal, November 2013

  • Kawasaki, Kazuhiko; Amemiya, Chris T.
  • Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Vol. 322, Issue 6
  • DOI: 10.1002/jez.b.22546

Earliest enamel deposits of the rat incisor examined by electron microscopy, electron diffraction, and electron probe microanalysis
journal, March 1988

  • Landis, William J.; Burke, Grace Y.; Neuringer, Julia R.
  • The Anatomical Record, Vol. 220, Issue 3
  • DOI: 10.1002/ar.1092200303

Partial expression of sex-linked recessive amelogenesis imperfecta in females compatible with the Lyon hypothesis
journal, February 1967

Construction and identification of mouse amelogenin cDNA clones.
journal, December 1983

  • Snead, M. L.; Zeichner-David, M.; Chandra, T.
  • Proceedings of the National Academy of Sciences, Vol. 80, Issue 23
  • DOI: 10.1073/pnas.80.23.7254

Isolation and characterization of a mouse amelogenin expressed in Escherichia coli
journal, April 1994

  • Simmer, J. P.; Lau, E. C.; Hu, C. C.
  • Calcified Tissue International, Vol. 54, Issue 4
  • DOI: 10.1007/BF00295956

Conservation of Amelogenin Gene Expression During Tetrapod Evolution: AMELOGENIN GENE EXPRESSION IN
journal, March 2013

  • Assaraf-Weill, Nathalie; Gasse, Barbara; Al-Hashimi, Nawfal
  • Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Vol. 320, Issue 4
  • DOI: 10.1002/jez.b.22494

Characterization of Recombinant Pig Enamelysin Activity and Cleavage of Recombinant Pig and Mouse Amelogenins
journal, March 1999

The Development of Enamel Structure
journal, September 1967

SEM evidence that one ameloblast secretes one keyhole-shaped enamel rod in monkey teeth
journal, May 2006

The Structural Biology of the Developing Dental Enamel Matrix
journal, June 1999

  • Fincham, A. G.; Moradian-Oldak, J.; Simmer, J. P.
  • Journal of Structural Biology, Vol. 126, Issue 3
  • DOI: 10.1006/jsbi.1999.4130

Dental enamel matrix: Sequences of two amelogenin polypeptides
journal, October 1981

  • Fincham, A. G.; Belcourt, A. B.; Termine, J. D.
  • Bioscience Reports, Vol. 1, Issue 10
  • DOI: 10.1007/bf01114799

Relationships between protein and mineral during enamel development in normal and genetically altered mice: Protein and enamel mineralization
journal, December 2011

A comparison of enamelin and amelogenin expression in developing mouse molars
journal, April 2001

The Effects of LAMP1 and LAMP3 on M180 Amelogenin Uptake, Localization and Amelogenin mRNA Induction by Amelogenin Protein
journal, June 2008

  • Xu, L.; Harada, H.; Taniguchi, A.
  • Journal of Biochemistry, Vol. 144, Issue 4
  • DOI: 10.1093/jb/mvn096

A Jurassic eutherian mammal and divergence of marsupials and placentals
journal, August 2011

  • Luo, Zhe-Xi; Yuan, Chong-Xi; Meng, Qing-Jin
  • Nature, Vol. 476, Issue 7361
  • DOI: 10.1038/nature10291

Genetic basis for the evolution of vertebrate mineralized tissue
journal, July 2004

  • Kawasaki, K.; Suzuki, T.; Weiss, K. M.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 31
  • DOI: 10.1073/pnas.0404279101

Potential Role of the Amelogenin N-Terminus in the Regulation of Calcium Phosphate Formation in vitro
journal, January 2011

  • Le Norcy, E.; Kwak, S. -Y.; Wiedemann-Bidlack, F. B.
  • Cells Tissues Organs, Vol. 194, Issue 2-4
  • DOI: 10.1159/000324827

Evolutionary Story of Mammalian-specific Amelogenin Exons 4, “4b”, 8, and 9
journal, September 2011

Critical roles for WDR72 in calcium transport and matrix protein removal during enamel maturation
journal, March 2015

  • Wang, Shih-Kai; Hu, Yuanyuan; Yang, Jie
  • Molecular Genetics & Genomic Medicine, Vol. 3, Issue 4
  • DOI: 10.1002/mgg3.143

Human and mouse amelogenin gene loci are on the sex chromosomes
journal, February 1989

Gene expression and immunolocalisation of amelogenins in developing embryonic and neonatal hamster teeth
journal, May 1997

  • Karg, H. A.; Burger, E. H.; Lyaruu, D. M.
  • Cell and Tissue Research, Vol. 288, Issue 3
  • DOI: 10.1007/s004410050840

Monoclonal antibody and immunogold cytochemical localization of amelogenins in bovine secretory amelogenesis
journal, January 1987

Molecular Phylogeny and Divergence Times of Deuterostome Animals
journal, July 2005

  • Blair, Jaime E.; Hedges, S. Blair
  • Molecular Biology and Evolution, Vol. 22, Issue 11
  • DOI: 10.1093/molbev/msi225

Amelogenesis Imperfecta in Two Families with Defined AMELX Deletions in ARHGAP6
journal, December 2012

Evidence for a single loss of mineralized teeth in the common avian ancestor
journal, December 2014

The Receptor Activator of Nuclear Factor-κB Ligand-mediated Osteoclastogenic Pathway Is Elevated in Amelogenin-null Mice
journal, July 2003

  • Hatakeyama, Junko; Sreenath, Taduru; Hatakeyama, Yuji
  • Journal of Biological Chemistry, Vol. 278, Issue 37
  • DOI: 10.1074/jbc.M306284200

Molecular Conformation of Porcine Amelogenin in Solution: Three Folding Units at the N-terminal, Central, and C-Terminal Regions1
journal, January 1993

Single Bovine Enamel Particles Examined by Electron Optics
journal, May 1984

The gar is a fish... is a bird... is a mammal?
journal, March 2016

Leucine-rich Amelogenin Peptides Regulate Mineralization in vitro
journal, May 2011

  • Le Norcy, E.; Kwak, S. -Y.; Wiedemann-Bidlack, F. B.
  • Journal of Dental Research, Vol. 90, Issue 9
  • DOI: 10.1177/0022034511411301

Amelogenesis Imperfecta: Genotype-Phenotype Studies in 71 Families
journal, January 2011

  • Wright, J. Timothy; Torain, Melody; Long, Kimberly
  • Cells Tissues Organs, Vol. 194, Issue 2-4
  • DOI: 10.1159/000324339

Blaschko Linear Enamel Defects - A Marker for Focal Dermal Hypoplasia: Case Report of Focal Dermal Hypoplasia
journal, May 2015

  • Gysin, Stefan; Itin, Peter
  • Case Reports in Dermatology, Vol. 7, Issue 2
  • DOI: 10.1159/000430781

Transient amorphous calcium phosphate in forming enamel
journal, May 2009

  • Beniash, Elia; Metzler, Rebecca A.; Lam, Raymond S. K.
  • Journal of Structural Biology, Vol. 166, Issue 2
  • DOI: 10.1016/j.jsb.2009.02.001

SEM evidence that one ameloblast secretes one keyhole-shaped enamel rod in monkey teeth
journal, May 2006

Enamelin Is Critical for Ameloblast Integrity and Enamel Ultrastructure Formation
journal, March 2014

Gene Action in the X-chromosome of the Mouse (Mus musculus L.)
journal, April 1961

New genomic and fossil data illuminate the origin of enamel
journal, September 2015

  • Qu, Qingming; Haitina, Tatjana; Zhu, Min
  • Nature, Vol. 526, Issue 7571
  • DOI: 10.1038/nature15259

Alteration of Conserved Alternative Splicing in AMELX Causes Enamel Defects
journal, August 2014

Ganoine Formation in the Scales of Primitive Actinopterygian Fishes, Lepisosteids and Polypterids
journal, January 1995

Expression of amelogenin mRNA sequences during development of rat molars
journal, January 1996

  • Wurtz, Tilmann; Lundmark, Carin; Christersson, Cecilia
  • Journal of Bone and Mineral Research, Vol. 11, Issue 1
  • DOI: 10.1002/jbmr.5650110117

TEM Observations on the Ameloblast/Enamel Interface in the Rat Incisor
journal, January 2002

  • Risnes, S.; Septier, D.; De Periere, D. Deville
  • Connective Tissue Research, Vol. 43, Issue 2-3
  • DOI: 10.1080/03008200290000899

Proteolytic activity of opossum tooth extracts
journal, January 1998

Mutational analysis of X-linked amelogenesis imperfecta in multiple families
journal, January 2000

Origin, Splicing, and Expression of Rodent Amelogenin Exon 8
journal, October 2006

Comparative Immunochemical Analyses of the Developmental Expression and Distribution of Ameloblastin and Amelogenin in Rat Incisors
journal, August 1998

Characterization of putative secretory sites on ameloblasts of the rat incisor
journal, October 1984

Integration of a hard x-ray microprobe with a diffractometer for microdiffraction
journal, March 2002

  • Libera, J.; Cai, Z.; Lai, B.
  • Review of Scientific Instruments, Vol. 73, Issue 3
  • DOI: 10.1063/1.1435815

The Structural Biology of the Developing Dental Enamel Matrix
journal, June 1999

  • Fincham, A. G.; Moradian-Oldak, J.; Simmer, J. P.
  • Journal of Structural Biology, Vol. 126, Issue 3
  • DOI: 10.1006/jsbi.1999.4130

A large interstitial deletion encompassing the amelogenin gene on the short arm of the Y chromosome
journal, February 2005

  • Lattanzi, Wanda; Di Giacomo, Marilena C.; Lenato, Gennaro M.
  • Human Genetics, Vol. 116, Issue 5
  • DOI: 10.1007/s00439-004-1238-z

Molecular cloning and mRNA tissue distribution of a novel matrix metalloproteinase isolated from porcine enamel organ
journal, January 1996

Spatial- and temporal-restricted pattern for amelogenin gene expression during mouse molar tooth organogenesis
journal, September 1988

  • Snead, Malcolm L.; Luo, Wen; Lau, Eduardo C.
  • Development, Vol. 104, Issue 1
  • DOI: 10.1242/dev.104.1.77
    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    Trafficking and secretion of keratin 75 by ameloblasts in vivo
    journal, October 2019

    • Yang, Xu; Yamazaki, Hajime; Yamakoshi, Yasuo
    • Journal of Biological Chemistry, Vol. 294, Issue 48
    • DOI: 10.1074/jbc.ra119.010037

    AMBN mutations causing hypoplastic amelogenesis imperfecta and Ambn knockout‐NLS‐lacZ knockin mice exhibiting failed amelogenesis and Ambn tissue‐specificity
    journal, July 2019

    • Liang, Tian; Hu, Yuanyuan; Smith, Charles E.
    • Molecular Genetics & Genomic Medicine, Vol. 7, Issue 9
    • DOI: 10.1002/mgg3.929

    Proteolysis by MMP20 Prevents Aberrant Mineralization in Secretory Enamel
    journal, February 2019

    Dental Enamel Formation and Implications for Oral Health and Disease
    journal, July 2017

    • Lacruz, Rodrigo S.; Habelitz, Stefan; Wright, J. Timothy
    • Physiological Reviews, Vol. 97, Issue 3
    • DOI: 10.1152/physrev.00030.2016

    The Evolution of Unusually Small Amelogenin Genes in Cetaceans; Pseudogenization, X–Y Gene Conversion, and Feeding Strategy
    journal, November 2019

    • Kawasaki, Kazuhiko; Mikami, Masato; Goto, Mutsuo
    • Journal of Molecular Evolution, Vol. 88, Issue 2
    • DOI: 10.1007/s00239-019-09917-0

    Endocytosis and Enamel Formation
    journal, July 2017

    Endocytosis and Enamel Formation
    journal, July 2017

      Sort by:
      [ × clear filter / sort ]

      Similar Records in DOE PAGES and OSTI.GOV collections: