Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy
Abstract
Here, we show that using infiltration of ZnO metal oxide can be useful for high resolution imaging of biological samples in electron and X-ray microscopy. This method is compatible with standard fixation techniques that leave the sample dry, such as finishing with super critical CO2 drying, or simple vacuum drying at 95°C. We demonstrate this technique can be applied on tooth and brain tissue samples. We also show that high resolution X-ray tomography can be performed on biological systems using Zn K edge (1s) absorption to enhance internal structures, and obtained the first nanoscale 10 KeV X-ray absorption images of the interior regions of a tooth.
- Authors:
-
[1];
- Argonne National Lab. (ANL), Argonne, IL (United States). Center for Nanoscale Materials
- Univ. of Chicago, IL (United States). Dept. of Neurbiology
- Univ. of Chicago, IL (United States). Dept. of Neurbiology; Argonne National Lab. (ANL), Argonne, IL (United States). Nanoscience and Technology Division
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1393905
- Grant/Contract Number:
- AC02-06CH11357; D16P00002
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Ocola, L. E., Sampathkumar, V., Kasthuri, N., and Winarski, R. P. Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy. United States: N. p., 2017.
Web. doi:10.1038/s41598-017-05690-6.
Ocola, L. E., Sampathkumar, V., Kasthuri, N., & Winarski, R. P. Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy. United States. https://doi.org/10.1038/s41598-017-05690-6
Ocola, L. E., Sampathkumar, V., Kasthuri, N., and Winarski, R. P. Wed .
"Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy". United States. https://doi.org/10.1038/s41598-017-05690-6. https://www.osti.gov/servlets/purl/1393905.
@article{osti_1393905,
title = {Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy},
author = {Ocola, L. E. and Sampathkumar, V. and Kasthuri, N. and Winarski, R. P.},
abstractNote = {Here, we show that using infiltration of ZnO metal oxide can be useful for high resolution imaging of biological samples in electron and X-ray microscopy. This method is compatible with standard fixation techniques that leave the sample dry, such as finishing with super critical CO2 drying, or simple vacuum drying at 95°C. We demonstrate this technique can be applied on tooth and brain tissue samples. We also show that high resolution X-ray tomography can be performed on biological systems using Zn K edge (1s) absorption to enhance internal structures, and obtained the first nanoscale 10 KeV X-ray absorption images of the interior regions of a tooth.},
doi = {10.1038/s41598-017-05690-6},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {Wed Jul 19 00:00:00 EDT 2017},
month = {Wed Jul 19 00:00:00 EDT 2017}
}
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