The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2
Abstract
Traditional marker-based mapping and next-generation sequencing was used to determine that the Arabidopsis (Arabidopsis thaliana) low cell wall arabinose mutant murus5 (mur5) encodes a defective allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2 (RGP2). Marker analysis of 13 F2 confirmed mutant progeny from a recombinant mapping population gave a rough map position on the upper arm of chromosome 5, and deep sequencing of DNA from these 13 lines gave five candidate genes with G→A (C→T) transitions predicted to result in amino acid changes. Of these five, only insertional mutant alleles of RGP2, a gene that encodes a UDP-arabinose mutase that interconverts UDP-arabinopyranose and UDP-arabinofuranose, exhibited the low cell wall arabinose phenotype. The identities of mur5 and two SALK insertional alleles were confirmed by allelism tests and overexpression of wild-type RGP2 complementary DNA placed under the control of the 35S promoter in the three alleles. The mur5 mutation results in the conversion of cysteine-257 to tyrosine-257 within a conserved hydrophobic cluster predicted to be distal to the active site and essential for protein stability and possible heterodimerization with other isoforms of RGP.
- Authors:
-
- Purdue Univ., West Lafayette, IN (United States)
- Univ. de Picardie Jules Verne, Amiens (France)
- Heidelberg Inst. für Theoretische Studien, Molecular Biomechanics, Heidelberg (Germany)
- Univ. of Connecticut, Storrs, CT (United States). Dept. of Molecular and Cell Biology
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1388829
- Grant/Contract Number:
- SC000997
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Plant Physiology (Bethesda)
- Additional Journal Information:
- Journal Name: Plant Physiology (Bethesda); Journal Volume: 171; Journal Issue: 3; Related Information: C3Bio partners with Purdue University (lead); Argonne National Laboratory; National Renewable Energy Laboratory; Northeastern University; University of Tennessee; Journal ID: ISSN 0032-0889
- Publisher:
- American Society of Plant Biologists
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; catalysis (homogeneous); catalysis (heterogeneous); biofuels (including algae and biomass); bio-inspired; materials and chemistry by design; synthesis (self-assembly); synthesis (scalable processing)
Citation Formats
Dugard, Christopher K., Mertz, Rachel A., Rayon, Catherine, Mercadante, Davide, Hart, Christopher, Benatti, Matheus R., Olek, Anna T., SanMiguel, Phillip J., Cooper, Bruce R., Reiter, Wolf-Dieter, McCann, Maureen C., and Carpita, Nicholas C. The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2. United States: N. p., 2016.
Web. doi:10.1104/pp.15.01922.
Dugard, Christopher K., Mertz, Rachel A., Rayon, Catherine, Mercadante, Davide, Hart, Christopher, Benatti, Matheus R., Olek, Anna T., SanMiguel, Phillip J., Cooper, Bruce R., Reiter, Wolf-Dieter, McCann, Maureen C., & Carpita, Nicholas C. The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2. United States. https://doi.org/10.1104/pp.15.01922
Dugard, Christopher K., Mertz, Rachel A., Rayon, Catherine, Mercadante, Davide, Hart, Christopher, Benatti, Matheus R., Olek, Anna T., SanMiguel, Phillip J., Cooper, Bruce R., Reiter, Wolf-Dieter, McCann, Maureen C., and Carpita, Nicholas C. Sat .
"The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2". United States. https://doi.org/10.1104/pp.15.01922. https://www.osti.gov/servlets/purl/1388829.
@article{osti_1388829,
title = {The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2},
author = {Dugard, Christopher K. and Mertz, Rachel A. and Rayon, Catherine and Mercadante, Davide and Hart, Christopher and Benatti, Matheus R. and Olek, Anna T. and SanMiguel, Phillip J. and Cooper, Bruce R. and Reiter, Wolf-Dieter and McCann, Maureen C. and Carpita, Nicholas C.},
abstractNote = {Traditional marker-based mapping and next-generation sequencing was used to determine that the Arabidopsis (Arabidopsis thaliana) low cell wall arabinose mutant murus5 (mur5) encodes a defective allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2 (RGP2). Marker analysis of 13 F2 confirmed mutant progeny from a recombinant mapping population gave a rough map position on the upper arm of chromosome 5, and deep sequencing of DNA from these 13 lines gave five candidate genes with G→A (C→T) transitions predicted to result in amino acid changes. Of these five, only insertional mutant alleles of RGP2, a gene that encodes a UDP-arabinose mutase that interconverts UDP-arabinopyranose and UDP-arabinofuranose, exhibited the low cell wall arabinose phenotype. The identities of mur5 and two SALK insertional alleles were confirmed by allelism tests and overexpression of wild-type RGP2 complementary DNA placed under the control of the 35S promoter in the three alleles. The mur5 mutation results in the conversion of cysteine-257 to tyrosine-257 within a conserved hydrophobic cluster predicted to be distal to the active site and essential for protein stability and possible heterodimerization with other isoforms of RGP.},
doi = {10.1104/pp.15.01922},
journal = {Plant Physiology (Bethesda)},
number = 3,
volume = 171,
place = {United States},
year = {Sat Jul 23 00:00:00 EDT 2016},
month = {Sat Jul 23 00:00:00 EDT 2016}
}
Web of Science
Figures / Tables:
Works referencing / citing this record:
Molecular characteristics of plant UDP-arabinopyranose mutases
journal, May 2019
- Saqib, Anam; Scheller, Henrik Vibe; Fredslund, Folmer
- Glycobiology, Vol. 29, Issue 12
Abscisic acid positively regulates l ‐arabinose metabolism to inhibit seed germination through ABSCISIC ACID INSENSITIVE4‐mediated transcriptional promotions of MUR4 in Arabidopsis thaliana
journal, September 2019
- Yan, Jingwei; Fang, Lin; Yang, Lan
- New Phytologist, Vol. 225, Issue 2
Molecular characteristics of plant UDP-arabinopyranose mutases
journal, May 2019
- Saqib, Anam; Scheller, Henrik Vibe; Fredslund, Folmer
- Glycobiology, Vol. 29, Issue 12