Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids
Abstract
ABSTRACT Monoterpenes (C 10 isoprenoids) are the main components of essential oils and are possible precursors for many commodity chemicals and high energy density fuels. Monoterpenes are synthesized from geranyl diphosphate (GPP), which is also the precursor for the biosynthesis of farnesyl diphosphate (FPP). FPP biosynthesis diverts the carbon flux from monoterpene production to C 15 products and quinone biosynthesis. In this study, we tested a chromosomal mutation of Escherichia coli 's native FPP synthase (IspA) to improve GPP availability for the production of monoterpenes using a heterologous mevalonate pathway. Monoterpene production at high levels required not only optimization of GPP production but also a basal level of FPP to maintain growth. The optimized strains produced two jet fuel precursor monoterpenoids 1,8‐cineole and linalool at the titer of 653 mg/L and 505 mg/L, respectively, in batch cultures with 1% glucose. The engineered strains developed in this work provide useful resources for the production of high‐value monoterpenes. Biotechnol. Bioeng. 2017;114: 1703–1712. © 2017 Wiley Periodicals, Inc.
- Authors:
-
[1]
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Technical Univ. of Denmark, Horsholm (Denmark); Univ. of California, Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1379914
- Alternate Identifier(s):
- OSTI ID: 1401291
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology and Bioengineering
- Additional Journal Information:
- Journal Volume: 114; Journal Issue: 8; Journal ID: ISSN 0006-3592
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Mendez-Perez, Daniel, Alonso-Gutierrez, Jorge, Hu, Qijun, Molinas, Margaux, Baidoo, Edward E. K., Wang, George, Chan, Leanne J. G., Adams, Paul D., Petzold, Christopher J., Keasling, Jay D., and Lee, Taek S. Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids. United States: N. p., 2017.
Web. doi:10.1002/bit.26296.
Mendez-Perez, Daniel, Alonso-Gutierrez, Jorge, Hu, Qijun, Molinas, Margaux, Baidoo, Edward E. K., Wang, George, Chan, Leanne J. G., Adams, Paul D., Petzold, Christopher J., Keasling, Jay D., & Lee, Taek S. Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids. United States. https://doi.org/10.1002/bit.26296
Mendez-Perez, Daniel, Alonso-Gutierrez, Jorge, Hu, Qijun, Molinas, Margaux, Baidoo, Edward E. K., Wang, George, Chan, Leanne J. G., Adams, Paul D., Petzold, Christopher J., Keasling, Jay D., and Lee, Taek S. Thu .
"Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids". United States. https://doi.org/10.1002/bit.26296. https://www.osti.gov/servlets/purl/1379914.
@article{osti_1379914,
title = {Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids},
author = {Mendez-Perez, Daniel and Alonso-Gutierrez, Jorge and Hu, Qijun and Molinas, Margaux and Baidoo, Edward E. K. and Wang, George and Chan, Leanne J. G. and Adams, Paul D. and Petzold, Christopher J. and Keasling, Jay D. and Lee, Taek S.},
abstractNote = {ABSTRACT Monoterpenes (C 10 isoprenoids) are the main components of essential oils and are possible precursors for many commodity chemicals and high energy density fuels. Monoterpenes are synthesized from geranyl diphosphate (GPP), which is also the precursor for the biosynthesis of farnesyl diphosphate (FPP). FPP biosynthesis diverts the carbon flux from monoterpene production to C 15 products and quinone biosynthesis. In this study, we tested a chromosomal mutation of Escherichia coli 's native FPP synthase (IspA) to improve GPP availability for the production of monoterpenes using a heterologous mevalonate pathway. Monoterpene production at high levels required not only optimization of GPP production but also a basal level of FPP to maintain growth. The optimized strains produced two jet fuel precursor monoterpenoids 1,8‐cineole and linalool at the titer of 653 mg/L and 505 mg/L, respectively, in batch cultures with 1% glucose. The engineered strains developed in this work provide useful resources for the production of high‐value monoterpenes. Biotechnol. Bioeng. 2017;114: 1703–1712. © 2017 Wiley Periodicals, Inc.},
doi = {10.1002/bit.26296},
journal = {Biotechnology and Bioengineering},
number = 8,
volume = 114,
place = {United States},
year = {Thu May 18 00:00:00 EDT 2017},
month = {Thu May 18 00:00:00 EDT 2017}
}
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