Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae
Abstract
Background: The rice receptor kinase XA21 confers robust resistance to the bacterial pathogenXanthomonas oryzae pv.oryzae(Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag (Ubi-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. Methods: To determine if the predicted NLS is required for XA21-mediated immunityin planta, we generated transgenic plants overexpressing an XA21 variant carrying the NLS with the same alanine substitutions (Ubi-XA21nls-GFP). Results: Ubi- XA21nls-GFP plants displayed slightly longer lesion lengths, higherXoo bacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi- XA21-GFP control plants. However, the Ubi- XA21nls-GFP plants express lower levels of protein than that observed inUbi- XA21-GFP. Discussion: These results demonstrate that the predicted NLS is not required for XA21-mediated immunity.
- Authors:
-
- Univ. of California, Davis, CA (United States). Department of Plant Pathology and the Genome Center; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Feedstocks Division, Joint BioEnergy Institute and Biological Systems and Engineering Division
- Univ. of California, Davis, CA (United States). Department of Plant Pathology and the Genome Center
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1378728
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PeerJ
- Additional Journal Information:
- Journal Volume: 4; Journal Issue: 10; Journal ID: ISSN 2167-8359
- Publisher:
- PeerJ Inc.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Nuclear localization; Bacterial blight disease; Xanthomonas oryzae pv. oryzae; XA21-mediated immunity
Citation Formats
Wei, Tong, Chen, Tsung-Chi, Ho, Yuen Ting, and Ronald, Pamela C. Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae. United States: N. p., 2016.
Web. doi:10.7717/peerj.2507.
Wei, Tong, Chen, Tsung-Chi, Ho, Yuen Ting, & Ronald, Pamela C. Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae. United States. https://doi.org/10.7717/peerj.2507
Wei, Tong, Chen, Tsung-Chi, Ho, Yuen Ting, and Ronald, Pamela C. Wed .
"Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae". United States. https://doi.org/10.7717/peerj.2507. https://www.osti.gov/servlets/purl/1378728.
@article{osti_1378728,
title = {Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae},
author = {Wei, Tong and Chen, Tsung-Chi and Ho, Yuen Ting and Ronald, Pamela C.},
abstractNote = {Background: The rice receptor kinase XA21 confers robust resistance to the bacterial pathogenXanthomonas oryzae pv.oryzae(Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag (Ubi-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. Methods: To determine if the predicted NLS is required for XA21-mediated immunityin planta, we generated transgenic plants overexpressing an XA21 variant carrying the NLS with the same alanine substitutions (Ubi-XA21nls-GFP). Results: Ubi- XA21nls-GFP plants displayed slightly longer lesion lengths, higherXoo bacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi- XA21-GFP control plants. However, the Ubi- XA21nls-GFP plants express lower levels of protein than that observed inUbi- XA21-GFP. Discussion: These results demonstrate that the predicted NLS is not required for XA21-mediated immunity.},
doi = {10.7717/peerj.2507},
journal = {PeerJ},
number = 10,
volume = 4,
place = {United States},
year = {Wed Oct 05 00:00:00 EDT 2016},
month = {Wed Oct 05 00:00:00 EDT 2016}
}
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Works referenced in this record:
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journal, January 2014
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Rice XA21 Binding Protein 3 Is a Ubiquitin Ligase Required for Full Xa21 -Mediated Disease Resistance
journal, December 2006
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The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium
journal, July 2015
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A Receptor Kinase-Like Protein Encoded by the Rice Disease Resistance Gene, Xa21
journal, December 1995
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- Science, Vol. 270, Issue 5243
Plant Innate Immunity: Perception of Conserved Microbial Signatures
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Rice XB15, a Protein Phosphatase 2C, Negatively Regulates Cell Death and XA21-Mediated Innate Immunity
journal, September 2008
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Towards Establishment of a Rice Stress Response Interactome
journal, April 2011
- Seo, Young-Su; Chern, Mawsheng; Bartley, Laura E.
- PLoS Genetics, Vol. 7, Issue 4, Article No. e1002020
Overexpression of the Endoplasmic Reticulum Chaperone BiP3 Regulates XA21-Mediated Innate Immunity in Rice
journal, February 2010
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- PLoS ONE, Vol. 5, Issue 2
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journal, January 2014
- Bahar, Ofir; Pruitt, Rory; Luu, Dee Dee
- PeerJ, Vol. 2
Works referencing / citing this record:
Four tyrosine residues of the rice immune receptor XA21 are not required for interaction with the co-receptor OsSERK2 or resistance to Xanthomonas oryzae pv. oryzae
journal, January 2018
- Caddell, Daniel F.; Wei, Tong; Sharma, Sweta
- PeerJ, Vol. 6
Four tyrosine residues of the rice immune receptor XA21 are not required for interaction with the co-receptor OsSERK2 or resistance to Xanthomonas oryzae pv. oryzae
journal, January 2018
- Caddell, Daniel F.; Wei, Tong; Sharma, Sweta
- PeerJ, Vol. 6