The role of Rnf in ion gradient formation in Desulfovibrio alaskensis
Abstract
Rnf is a membrane protein complex that has been shown to be important in energy conservation. Here, Desulfovibrio alaskensis G20 and Rnf mutants of G20 were grown with different electron donor and acceptor combinations to determine the importance of Rnf in energy conservation and the type of ion gradient generated. The addition of the protonophore TCS strongly inhibited lactate-sulfate dependent growth whereas the sodium ionophore ETH2120 had no effect, indicating a role for the proton gradient during growth. Mutants in rnfA and rnfD were more sensitive to the protonophore at 5 µM than the parental strain, suggesting the importance of Rnf in the generation of a proton gradient. The electrical potential (ΔΨ), ΔpH and proton motive force were lower in thernfAmutant than in the parental strain of D.alaskensis G20. In conclusion, these results provide evidence that the Rnf complex in D. alaskensis functions as a primary proton pump whose activity is important for growth.
- Authors:
-
- Univ. of Oklahoma, Norman, OK (United States)
- Publication Date:
- Research Org.:
- Office of Scientific and Technical Information, Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1375918
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PeerJ
- Additional Journal Information:
- Journal Volume: 4; Journal ID: ISSN 2167-8359
- Publisher:
- PeerJ Inc.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Desulfovibrio; Rnf; proton motive force; ionophore; energetics
Citation Formats
Wang, Luyao, Bradstock, Peter, Li, Chuang, McInerney, Michael J., and Krumholz, Lee R. The role of Rnf in ion gradient formation in Desulfovibrio alaskensis. United States: N. p., 2016.
Web. doi:10.7717/peerj.1919.
Wang, Luyao, Bradstock, Peter, Li, Chuang, McInerney, Michael J., & Krumholz, Lee R. The role of Rnf in ion gradient formation in Desulfovibrio alaskensis. United States. https://doi.org/10.7717/peerj.1919
Wang, Luyao, Bradstock, Peter, Li, Chuang, McInerney, Michael J., and Krumholz, Lee R. Thu .
"The role of Rnf in ion gradient formation in Desulfovibrio alaskensis". United States. https://doi.org/10.7717/peerj.1919. https://www.osti.gov/servlets/purl/1375918.
@article{osti_1375918,
title = {The role of Rnf in ion gradient formation in Desulfovibrio alaskensis},
author = {Wang, Luyao and Bradstock, Peter and Li, Chuang and McInerney, Michael J. and Krumholz, Lee R.},
abstractNote = {Rnf is a membrane protein complex that has been shown to be important in energy conservation. Here, Desulfovibrio alaskensis G20 and Rnf mutants of G20 were grown with different electron donor and acceptor combinations to determine the importance of Rnf in energy conservation and the type of ion gradient generated. The addition of the protonophore TCS strongly inhibited lactate-sulfate dependent growth whereas the sodium ionophore ETH2120 had no effect, indicating a role for the proton gradient during growth. Mutants in rnfA and rnfD were more sensitive to the protonophore at 5 µM than the parental strain, suggesting the importance of Rnf in the generation of a proton gradient. The electrical potential (ΔΨ), ΔpH and proton motive force were lower in thernfAmutant than in the parental strain of D.alaskensis G20. In conclusion, these results provide evidence that the Rnf complex in D. alaskensis functions as a primary proton pump whose activity is important for growth.},
doi = {10.7717/peerj.1919},
journal = {PeerJ},
number = ,
volume = 4,
place = {United States},
year = {Thu Apr 14 00:00:00 EDT 2016},
month = {Thu Apr 14 00:00:00 EDT 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 6 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Biochemistry, evolution and physiological function of the Rnf complex, a novel ion-motive electron transport complex in prokaryotes
journal, November 2010
- Biegel, Eva; Schmidt, Silke; González, José M.
- Cellular and Molecular Life Sciences, Vol. 68, Issue 4
Occurrence of ferredoxin:NAD + oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria
journal, January 2016
- Hess, Verena; Gallegos, Rene; Jones, J. Andrew
- PeerJ, Vol. 4
Volume regulation in Mycoplasma gallisepticum: evidence that Na+ is extruded via a primary Na+ pump.
journal, August 1989
- Shirvan, M. H.; Schuldiner, S.; Rottem, S.
- Journal of Bacteriology, Vol. 171, Issue 8
Spectrophotometric Determination Of Hydrogen Sulfide In Natural Waters
journal, May 1969
- Cline, Joel D.
- Limnology and Oceanography, Vol. 14, Issue 3, p. 454-458
Volume regulation in Mycoplasma gallisepticum: evidence that Na+ is extruded via a primary Na+ pump.
journal, August 1989
- Shirvan, M. H.; Schuldiner, S.; Rottem, S.
- Journal of Bacteriology, Vol. 171, Issue 8
A Periplasmic Location Is Essential for the Role of the ApbE Lipoprotein in Thiamine Synthesis in Salmonella typhimurium
journal, December 1999
- Beck, Brian J.; Downs, Diana M.
- Journal of Bacteriology, Vol. 181, Issue 23
Biochemistry, evolution and physiological function of the Rnf complex, a novel ion-motive electron transport complex in prokaryotes
journal, November 2010
- Biegel, Eva; Schmidt, Silke; González, José M.
- Cellular and Molecular Life Sciences, Vol. 68, Issue 4
Metabolism of H2 by Desulfovibrio alaskensis G20 during syntrophic growth on lactate
journal, July 2011
- Li, X.; McInerney, M. J.; Stahl, D. A.
- Microbiology, Vol. 157, Issue 10
Genes required for rapid expression of nitrogenase activity in Azotobacter vinelandii
journal, April 2005
- Curatti, L.; Brown, C. S.; Ludden, P. W.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 18
Membrane Localization, Topology, and Mutual Stabilization of the rnfABC Gene Products in Rhodobacter capsulatus and Implications for a New Family of Energy-Coupling NADH Oxidoreductases †
journal, May 1997
- Kumagai, Hirotaka; Fujiwara, Takeshi; Matsubara, Hiroshi
- Biochemistry, Vol. 36, Issue 18
The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features
journal, January 2008
- Seedorf, H.; Fricke, W. F.; Veith, B.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 6
The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features
journal, January 2008
- Seedorf, H.; Fricke, W. F.; Veith, B.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 6
Uncouplers and the molecular mechanism of uncoupling in mitochondria.
journal, June 1977
- Kessler, R. J.; Vande Zande, H.; Tyson, C. A.
- Proceedings of the National Academy of Sciences, Vol. 74, Issue 6
A Method Adapting Microarray Technology for Signature-Tagged Mutagenesis of Desulfovibrio desulfuricans G20 and Shewanella oneidensis MR-1 in Anaerobic Sediment Survival Experiments
journal, November 2005
- Groh, J. L.; Luo, Q.; Ballard, J. D.
- Applied and Environmental Microbiology, Vol. 71, Issue 11
Effects of potassium ions on the electrical and pH gradients across the membrane of Streptococcus lactis cells.
journal, January 1977
- Kashket, E. R.; Barker, S. L.
- Journal of Bacteriology, Vol. 130, Issue 3
Bacterial Na+-translocating ferredoxin:NAD+ oxidoreductase
journal, October 2010
- Biegel, E.; Muller, V.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 42
Bacterial Na+-translocating ferredoxin:NAD+ oxidoreductase
journal, October 2010
- Biegel, E.; Muller, V.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 42
Genetic, immunological and biochemical evidence for a Rnf complex in the acetogen Acetobacterium woodii
journal, June 2009
- Biegel, Eva; Schmidt, Silke; Müller, Volker
- Environmental Microbiology, Vol. 11, Issue 6
Sulphate respiration from hydrogen in bacteria: a structural biology overview
journal, November 2005
- Matias, P.; Pereira, I.; Soares, C.
- Progress in Biophysics and Molecular Biology, Vol. 89, Issue 3
Overexpression in Escherichia coli of the rnf genes from Rhodobacter capsulatus . Characterization of two membrane-bound iron-sulfur proteins
journal, January 1998
- Jouanneau, Yves; Jeong, Ho-Sang; Hugo, Nicolas
- European Journal of Biochemistry, Vol. 251, Issue 1-2
Metabolism of H2 by Desulfovibrio alaskensis G20 during syntrophic growth on lactate
journal, July 2011
- Li, X.; McInerney, M. J.; Stahl, D. A.
- Microbiology, Vol. 157, Issue 10
The Rnf Complex of Clostridium ljungdahlii Is a Proton-Translocating Ferredoxin:NAD+ Oxidoreductase Essential for Autotrophic Growth
journal, December 2012
- Tremblay, Pier-Luc; Zhang, Tian; Dar, Shabir A.
- mBio, Vol. 4, Issue 1
A new mathematical model for relative quantification in real-time RT-PCR
journal, May 2001
- Pfaffl, M. W.
- Nucleic Acids Research, Vol. 29, Issue 9
A Comparative Genomic Analysis of Energy Metabolism in Sulfate Reducing Bacteria and Archaea
journal, January 2011
- Pereira, Inês A. Cardoso; Ramos, Ana Raquel; Grein, Fabian
- Frontiers in Microbiology, Vol. 2
Spectrophotometric Determination Of Hydrogen Sulfide In Natural Waters
journal, May 1969
- Cline, Joel D.
- Limnology and Oceanography, Vol. 14, Issue 3, p. 454-458
Determinación y cuantificación de bacterias acidolácticas por PCR en tiempo real
journal, January 2010
- Phandanouvong, Vienvilay; Betancourt, Liliana; Rodriguez, Fernando
- Revista MVZ Córdoba, Vol. 15, Issue 1
Clostridium ljungdahlii represents a microbial production platform based on syngas
journal, July 2010
- Kopke, M.; Held, C.; Hujer, S.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 29, p. 13087-13092
Clostridium ljungdahlii represents a microbial production platform based on syngas
journal, July 2010
- Kopke, M.; Held, C.; Hujer, S.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 29, p. 13087-13092
A Comparative Genomic Analysis of Energy Metabolism in Sulfate Reducing Bacteria and Archaea
journal, January 2011
- Pereira, Inês A. Cardoso; Ramos, Ana Raquel; Grein, Fabian
- Frontiers in Microbiology, Vol. 2
Syntrophic Growth of Desulfovibrio alaskensis Requires Genes for H 2 and Formate Metabolism as Well as Those for Flagellum and Biofilm Formation
journal, January 2015
- Krumholz, Lee R.; Bradstock, Peter; Sheik, Cody S.
- Applied and Environmental Microbiology, Vol. 81, Issue 7
Sodium Ion Pumps and Hydrogen Production in Glutamate Fermenting Anaerobic Bacteria
journal, January 2005
- Boiangiu, Clara D.; Jayamani, Elamparithi; Brügel, Daniela
- Journal of Molecular Microbiology and Biotechnology, Vol. 10, Issue 2-4
Overexpression in Escherichia coli of the rnf genes from Rhodobacter capsulatus . Characterization of two membrane-bound iron-sulfur proteins
journal, January 1998
- Jouanneau, Yves; Jeong, Ho-Sang; Hugo, Nicolas
- European Journal of Biochemistry, Vol. 251, Issue 1-2
Sulphate respiration from hydrogen in bacteria: a structural biology overview
journal, November 2005
- Matias, P.; Pereira, I.; Soares, C.
- Progress in Biophysics and Molecular Biology, Vol. 89, Issue 3
The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20
journal, October 2014
- Price, Morgan N.; Ray, Jayashree; Wetmore, Kelly M.
- Frontiers in Microbiology, Vol. 5
Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501
journal, May 2008
- Yan, Y.; Yang, J.; Dou, Y.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 21
Energy conservation via electron bifurcating ferredoxin reduction and proton/Na+ translocating ferredoxin oxidation
journal, February 2013
- Buckel, Wolfgang; Thauer, Rudolf K.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 2
Genetic, immunological and biochemical evidence for a Rnf complex in the acetogen Acetobacterium woodii
journal, June 2009
- Biegel, Eva; Schmidt, Silke; Müller, Volker
- Environmental Microbiology, Vol. 11, Issue 6
Discovery of a Ferredoxin:NAD+-Oxidoreductase (Rnf) in Acetobacterium woodii: A Novel Potential Coupling Site in Acetogens
journal, March 2008
- Müller, Volker; Imkamp, Frank; Biegel, Eva
- Annals of the New York Academy of Sciences, Vol. 1125, Issue 1
ATP Sulfurylases from Sulfate-Reducing Bacteria of the Genus Desulfovibrio. A Novel Metalloprotein Containing Cobalt and Zinc †
journal, November 1998
- Gavel, Olga Yu.; Bursakov, Sergey A.; Calvete, Juan J.
- Biochemistry, Vol. 37, Issue 46
Identification of a new class of nitrogen fixation genes in Rhodobacter capsalatus: a putative membrane complex involved in electron transport to nitrogenase
journal, December 1993
- Schmehl, Manfred; Jahn, Andreas; Meyer zu Vilsendorf, Andreas
- MGG Molecular & General Genetics, Vol. 241-241, Issue 5-6
ATP Sulfurylases from Sulfate-Reducing Bacteria of the Genus Desulfovibrio. A Novel Metalloprotein Containing Cobalt and Zinc †
journal, November 1998
- Gavel, Olga Yu.; Bursakov, Sergey A.; Calvete, Juan J.
- Biochemistry, Vol. 37, Issue 46
Discovery of a Ferredoxin:NAD+-Oxidoreductase (Rnf) in Acetobacterium woodii: A Novel Potential Coupling Site in Acetogens
journal, March 2008
- Müller, Volker; Imkamp, Frank; Biegel, Eva
- Annals of the New York Academy of Sciences, Vol. 1125, Issue 1
Uncouplers and the molecular mechanism of uncoupling in mitochondria.
journal, June 1977
- Kessler, R. J.; Vande Zande, H.; Tyson, C. A.
- Proceedings of the National Academy of Sciences, Vol. 74, Issue 6
Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501
journal, May 2008
- Yan, Y.; Yang, J.; Dou, Y.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 21
Vectorial electron transport in Degulfovibrio vulgaris (Marburg) growing on hydrogen plus sulfate as sole energy source
journal, March 1980
- Badzjong, Werner; Thauer, Rudolf K.
- Archives of Microbiology, Vol. 125, Issue 1-2
Membrane Localization, Topology, and Mutual Stabilization of the rnfABC Gene Products in Rhodobacter capsulatus and Implications for a New Family of Energy-Coupling NADH Oxidoreductases †
journal, May 1997
- Kumagai, Hirotaka; Fujiwara, Takeshi; Matsubara, Hiroshi
- Biochemistry, Vol. 36, Issue 18
Energy conservation via electron bifurcating ferredoxin reduction and proton/Na+ translocating ferredoxin oxidation
journal, February 2013
- Buckel, Wolfgang; Thauer, Rudolf K.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 2
Redox properties of several bacterial ferredoxins using square wave voltammetry.
journal, August 1990
- Smith, E. T.; Feinberg, B. A.
- Journal of Biological Chemistry, Vol. 265, Issue 24
Works referencing / citing this record:
Homoacetogenesis in Deep-Sea Chloroflexi , as Inferred by Single-Cell Genomics, Provides a Link to Reductive Dehalogenation in Terrestrial Dehalococcoidetes
journal, December 2017
- Sewell, Holly L.; Kaster, Anne-Kristin; Spormann, Alfred M.
- mBio, Vol. 8, Issue 6