Intracellular distribution and stability of a luminescent rhenium(I) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging
Abstract
Optical fluorescence microscopy was used in conjunction with X-ray fluorescence microscopy to monitor the stability and intracellular distribution of the luminescent rhenium(I) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex, in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence techniques. Furthermore, X-ray fluorescence also showed that the Re-I complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.
- Authors:
-
[1];
- The Univ. of Adelaide (Australia)
- Univ. of South Australia, Adelaide (Australia)
- The Univ. of Sydney (Australia)
- Dept. of Chemistry, Perth (Australia)
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Univ. of Western Australia, Crawley (Australia)
- Univ. of Bologna, Bologna (Italy)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- Australian Research Council; USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1374605
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Metallomics
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 4; Journal ID: ISSN 1756-5901
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
Citation Formats
Wedding, Jason L., Harris, Hugh H., Bader, Christie A., Plush, Sally E., Mak, Rachel, Massi, Massimiliano, Brooks, Douglas A., Lai, Barry, Vogt, Stefan, Werrett, Melissa V., Simpson, Peter V., Skelton, Brian W., and Stagni, Stefano. Intracellular distribution and stability of a luminescent rhenium(I) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging. United States: N. p., 2016.
Web. doi:10.1039/c6mt00243a.
Wedding, Jason L., Harris, Hugh H., Bader, Christie A., Plush, Sally E., Mak, Rachel, Massi, Massimiliano, Brooks, Douglas A., Lai, Barry, Vogt, Stefan, Werrett, Melissa V., Simpson, Peter V., Skelton, Brian W., & Stagni, Stefano. Intracellular distribution and stability of a luminescent rhenium(I) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging. United States. https://doi.org/10.1039/c6mt00243a
Wedding, Jason L., Harris, Hugh H., Bader, Christie A., Plush, Sally E., Mak, Rachel, Massi, Massimiliano, Brooks, Douglas A., Lai, Barry, Vogt, Stefan, Werrett, Melissa V., Simpson, Peter V., Skelton, Brian W., and Stagni, Stefano. Wed .
"Intracellular distribution and stability of a luminescent rhenium(I) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging". United States. https://doi.org/10.1039/c6mt00243a. https://www.osti.gov/servlets/purl/1374605.
@article{osti_1374605,
title = {Intracellular distribution and stability of a luminescent rhenium(I) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging},
author = {Wedding, Jason L. and Harris, Hugh H. and Bader, Christie A. and Plush, Sally E. and Mak, Rachel and Massi, Massimiliano and Brooks, Douglas A. and Lai, Barry and Vogt, Stefan and Werrett, Melissa V. and Simpson, Peter V. and Skelton, Brian W. and Stagni, Stefano},
abstractNote = {Optical fluorescence microscopy was used in conjunction with X-ray fluorescence microscopy to monitor the stability and intracellular distribution of the luminescent rhenium(I) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex, in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence techniques. Furthermore, X-ray fluorescence also showed that the Re-I complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.},
doi = {10.1039/c6mt00243a},
journal = {Metallomics},
number = 4,
volume = 9,
place = {United States},
year = {Wed Nov 23 00:00:00 EST 2016},
month = {Wed Nov 23 00:00:00 EST 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 29 works
Citation information provided by
Web of Science
Web of Science
Figures / Tables:
Figure 1: Structure of the investgated complex Re-I.
All figures and tables
(7 total)
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Distinct cellular fates for KP1019 and NAMI-A determined by X-ray fluorescence imaging of single cells
journal, January 2012
- Aitken, Jade B.; Antony, Sumy; Weekley, Claire M.
- Metallomics, Vol. 4, Issue 10
Cyclic and acyclic polyamines as chelators of rhenium-186 and Rhenium-188 for therapeutic use
journal, May 1996
- Prakash, Sushumna; Went, Michael J.; Blower, Philip J.
- Nuclear Medicine and Biology, Vol. 23, Issue 4
Application of d6 transition metal complexes in fluorescence cell imaging
journal, January 2010
- Fernández-Moreira, Vanesa; Thorp-Greenwood, Flora L.; Coogan, Michael P.
- Chem. Commun., Vol. 46, Issue 2
Selenium Metabolism in Cancer Cells: The Combined Application of XAS and XFM Techniques to the Problem of Selenium Speciation in Biological Systems
journal, May 2013
- Weekley, Claire; Aitken, Jade; Finney, Lydia
- Nutrients, Vol. 5, Issue 5
Subcellular redistribution and mitotic inheritance of transition metals in proliferating mouse fibroblast cells
journal, January 2013
- McRae, Reagan; Lai, Barry; Fahrni, Christoph J.
- Metallomics, Vol. 5, Issue 1
Selenite-mediated production of superoxide radical anions in A549 cancer cells is accompanied by a selective increase in SOD1 concentration, enhanced apoptosis and Se–Cu bonding
journal, February 2014
- Weekley, Claire M.; Jeong, Gloria; Tierney, Michael E.
- JBIC Journal of Biological Inorganic Chemistry, Vol. 19, Issue 6
Novel Synthesis of 5-Substituted Tetrazoles from Nitriles
journal, June 1998
- Koguro, Kiyoto; Oga, Toshikazu; Mitsui, Sunao
- Synthesis, Vol. 1998, Issue 06
Current use and future potential of organometallic radiopharmaceuticals
journal, November 2002
- Schibli, Roger; Schubiger, August
- European Journal of Nuclear Medicine and Molecular Imaging, Vol. 29, Issue 11
Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time
journal, September 2016
- Bader, Christie A.; Sorvina, Alexandra; Simpson, Peter V.
- FEBS Letters, Vol. 590, Issue 18
Underestimated Potential of Organometallic Rhenium Complexes as Anticancer Agents
journal, August 2014
- Leonidova, Anna; Gasser, Gilles
- ACS Chemical Biology, Vol. 9, Issue 10
A Molecular Probe for the Detection of Polar Lipids in Live Cells
journal, August 2016
- Bader, Christie A.; Shandala, Tetyana; Carter, Elizabeth A.
- PLOS ONE, Vol. 11, Issue 8
Measurement of photoluminescence quantum yields. Review
journal, April 1971
- Crosby, Glenn A.; Demas, James N.
- The Journal of Physical Chemistry, Vol. 75, Issue 8, p. 991-1024
{Re III Cl 3 } Core Complexes with Bifunctional Single Amino Acid Chelates
journal, November 2002
- Banerjee, Sangeeta Ray; Wei, Lihui; Levadala, Murali K.
- Inorganic Chemistry, Vol. 41, Issue 22
Rhenium fac tricarbonyl bisimine complexes: biologically useful fluorochromes for cell imaging applications
journal, January 2007
- Amoroso, Angelo J.; Coogan, Michael P.; Dunne, Jennifer E.
- Chem. Commun., Issue 29
Synthesis, Structural, and Photophysical Investigation of Diimine Triscarbonyl Re(I) Tetrazolato Complexes.
journal, February 2011
- Werrett, Melissa V.; Chartrand, Daniel; Gale, Julian D.
- Inorganic Chemistry, Vol. 50, Issue 4
Unprecedented staining of polar lipids by a luminescent rhenium complex revealed by FTIR microspectroscopy in adipocytes
journal, January 2016
- Bader, C. A.; Carter, E. A.; Safitri, A.
- Molecular BioSystems, Vol. 12, Issue 7
Fluorescence probes for prokaryotic and eukaryotic cells using Re(CO) 3 + complexes with an electron withdrawing ancillary ligand
journal, January 2016
- Carreño, A.; Gacitúa, M.; Fuentes, J. A.
- New Journal of Chemistry, Vol. 40, Issue 9
Alkoxy bridged binuclear rhenium (I) complexes as a potential sensor for β-amyloid aggregation
journal, December 2014
- Sathish, Veerasamy; Babu, Eththilu; Ramdass, Arumugam
- Talanta, Vol. 130
Synthesis, Luminescence Quantum Yields, and Lifetimes of Trischelated Ruthenium(II) Mixed-ligand Complexes Including 3,3′-Dimethyl-2,2′-bipyridyl
journal, September 1982
- Nakamaru, Katsumi
- Bulletin of the Chemical Society of Japan, Vol. 55, Issue 9
Biologically Compatible, Phosphorescent Dimetallic Rhenium Complexes Linked through Functionalized Alkyl Chains: Syntheses, Spectroscopic Properties, and Applications in Imaging Microscopy
journal, January 2012
- Balasingham, Rebeca G.; Thorp-Greenwood, Flora L.; Williams, Catrin F.
- Inorganic Chemistry, Vol. 51, Issue 3
Applications of luminescent inorganic and organometallic transition metal complexes as biomolecular and cellular probes
journal, January 2012
- Lo, Kenneth Kam-Wing; Choi, Alex Wing-Tat; Law, Wendell Ho-Tin
- Dalton Transactions, Vol. 41, Issue 20
Uptake, Distribution, and Speciation of Selenoamino Acids by Human Cancer Cells: X-ray Absorption and Fluorescence Methods
journal, March 2011
- Weekley, Claire M.; Aitken, Jade B.; Vogt, Stefan
- Biochemistry, Vol. 50, Issue 10
The Structure of the Potassium Channel: Molecular Basis of K+ Conduction and Selectivity
journal, April 1998
- Doyle, D. A.
- Science, Vol. 280, Issue 5360
Induction of DNA-Double-Strand Breaks by Auger Electrons from 99mTc Complexes with DNA-Binding Ligands
journal, January 2005
- Häfliger, Pascal; Agorastos, Nikos; Spingler, Bernhard
- ChemBioChem, Vol. 6, Issue 2
Basic aqueous chemistry of [M(OH2)3(CO)3]+ (M=Re, Tc) directed towards radiopharmaceutical application
journal, September 1999
- Alberto, Roger; Schibli, Roger; Waibel, Robert
- Coordination Chemistry Reviews, Vol. 190-192
A Nonemissive Iridium(III) Complex That Specifically Lights-Up the Nuclei of Living Cells
journal, July 2011
- Li, Chunyan; Yu, Mengxiao; Sun, Yun
- Journal of the American Chemical Society, Vol. 133, Issue 29
Correlative microXRF and optical immunofluorescence microscopy of adherent cells labeled with ultrasmall gold particles
journal, July 2006
- McRae, Reagan; Lai, Barry; Vogt, Stefan
- Journal of Structural Biology, Vol. 155, Issue 1
Fluorescent Rhenium-Naphthalimide Conjugates as Cellular Imaging Agents
journal, March 2014
- Langdon-Jones, Emily E.; Symonds, Nadine O.; Yates, Sara E.
- Inorganic Chemistry, Vol. 53, Issue 7
Silicon nitride as a versatile growth substrate for microspectroscopic imaging and mapping of individual cells
journal, January 2010
- Carter, Elizabeth A.; Rayner, Benjamin S.; McLeod, Andrew I.
- Molecular BioSystems, Vol. 6, Issue 7
Synthesis and Characterization of Luminescent Rhenium(I) Tricarbonyl Diimine Complexes with a Triarylboron Moiety and the Study of Their Fluoride Ion-Binding Properties
journal, October 2009
- Lam, Siu-Tung; Zhu, Nianyong; Yam, Vivian Wing-Wah
- Inorganic Chemistry, Vol. 48, Issue 20
XAS and XFM studies of selenium and copper speciation and distribution in the kidneys of selenite-supplemented rats
journal, January 2014
- Weekley, Claire M.; Shanu, Anu; Aitken, Jade B.
- Metallomics, Vol. 6, Issue 9
Comprehensive Radiolabeling, Stability, and Tissue Distribution Studies of Technetium-99m Single Amino Acid Chelates (SAAC)
journal, August 2009
- Maresca, Kevin P.; Hillier, Shawn M.; Femia, Frank J.
- Bioconjugate Chemistry, Vol. 20, Issue 8
The one-pot synthesis and the fluorescence and cytotoxicity studies of chlorotricarbonyl(α-diimine)rhenium(I), fac-(CO)3(α-diimine)ReCl, complexes
journal, September 2008
- Orsa, Dejene K.; Haynes, Gregory K.; Pramanik, Saroj K.
- Inorganic Chemistry Communications, Vol. 11, Issue 9
Modulation of the organelle specificity in Re( i ) tetrazolato complexes leads to labeling of lipid droplets
journal, January 2014
- Bader, Christie A.; Brooks, Robert D.; Ng, Yeap S.
- RSC Adv., Vol. 4, Issue 31
A Phenylbenzothiazole Conjugate with the Tricarbonyl fac-[M(I)(CO)3]+ (M = Re, 99Tc, 99mTc) Core for Imaging of β-Amyloid Plaques
journal, August 2012
- Sagnou, Marina; Tzanopoulou, Stamatia; Raptopoulou, Catherine P.
- European Journal of Inorganic Chemistry, Vol. 2012, Issue 27
Novel Tat-Peptide Chelates for Direct Transduction of Technetium-99m and Rhenium into Human Cells for Imaging and Radiotherapy
journal, November 2000
- Polyakov, Valery; Sharma, Vijay; Dahlheimer, Julie L.
- Bioconjugate Chemistry, Vol. 11, Issue 6
Therapeutic Radiopharmaceuticals
journal, September 1999
- Volkert, Wynn A.; Hoffman, Timothy J.
- Chemical Reviews, Vol. 99, Issue 9
Organometallic Compounds: An Opportunity for Chemical Biology?
journal, May 2012
- Patra, Malay; Gasser, Gilles
- ChemBioChem, Vol. 13, Issue 9
The nature of the lowest excited state in fac-tricarbonylhalobis(4-phenylpyridine)rhenium(I) and fac-tricarbonylhalobis(4,4'-bipyridine)rhenium(I): emissive organometallic complexes in fluid solution
journal, May 1979
- Giordano, Paul J.; Wrighton, Mark S.
- Journal of the American Chemical Society, Vol. 101, Issue 11
Organometallic Anticancer Compounds
journal, January 2011
- Gasser, Gilles; Ott, Ingo; Metzler-Nolte, Nils
- Journal of Medicinal Chemistry, Vol. 54, Issue 1
Rhenium tetrazolato complexes coordinated to thioalkyl-functionalised phenanthroline ligands: synthesis, photophysical characterisation, and incubation in live HeLa cells
journal, January 2015
- Werrett, Melissa V.; Wright, Phillip J.; Simpson, Peter V.
- Dalton Transactions, Vol. 44, Issue 47
Developing the {M(CO) 3 } + Core for Fluorescence Applications: Rhenium Tricarbonyl Core Complexes with Benzimidazole, Quinoline, and Tryptophan Derivatives
journal, April 2006
- Wei, Lihui; Babich, John W.; Ouellette, Wayne
- Inorganic Chemistry, Vol. 45, Issue 7
Synthetic strategy for organometallic complexes of rhenium with exceptionally high affinity for the oestradiol receptor; their potential use as imaging and therapeutic agents
journal, January 1994
- Top, Siden; Vessières, Anne; Jaouen, Gérard
- J. Chem. Soc., Chem. Commun., Issue 4
Ultrafast Excited-State Dynamics of [Re(L)(CO) 3 (bpy)] n Complexes: Involvement of the Solvent
journal, May 2010
- El Nahhas, Amal; Cannizzo, Andrea; van Mourik, Frank
- The Journal of Physical Chemistry A, Vol. 114, Issue 22
Progress with, and prospects for, metal complexes in cell imaging
journal, January 2014
- Coogan, Michael P.; Fernández-Moreira, Vanesa
- Chem. Commun., Vol. 50, Issue 4
Protein and Peptide Labeling with (Cyclopentadienyl)tricarbonyl Rhenium and Technetium
journal, November 1998
- Spradau, Todd W.; Katzenellenbogen, John A.
- Bioconjugate Chemistry, Vol. 9, Issue 6
Luminescent Rhenium(I)-Dipyrrinato Complexes
journal, November 2011
- McLean, Tracey M.; Moody, Janice L.; Waterland, Mark R.
- Inorganic Chemistry, Vol. 51, Issue 1
N-(2-Mercaptoethyl)picolylamine as a Diaminomonothiolate Ligand for the “fac-[Re(CO)3]+” Core
journal, November 2002
- Kramer, Daniel J.; Davison, Alan; Davis, William M.
- Inorganic Chemistry, Vol. 41, Issue 24
Complexes in context: Attempting to control the cellular uptake and localisation of rhenium fac-tricarbonyl polypyridyl complexes
journal, January 2011
- Balasingham, Rebeca G.; Coogan, Michael P.; Thorp-Greenwood, Flora L.
- Dalton Transactions, Vol. 40, Issue 44
Metabolism of Selenite in Human Lung Cancer Cells: X-Ray Absorption and Fluorescence Studies
journal, November 2011
- Weekley, Claire M.; Aitken, Jade B.; Vogt, Stefan
- Journal of the American Chemical Society, Vol. 133, Issue 45
Luminescent Rhenium(I) and Iridium(III) Polypyridine Complexes as Biological Probes, Imaging Reagents, and Photocytotoxic Agents
journal, July 2015
- Lo, Kenneth Kam-Wing
- Accounts of Chemical Research, Vol. 48, Issue 12
Preface
journal, July 2000
- Tayan Ercan, Meral; Caglar, Meltem
- Current Pharmaceutical Design, Vol. 6, Issue 11
Organometallic anticancer compounds
text, January 2011
- Gasser, Gilles; Ott, Ingo; Metzler-Nolte, Nils
- American Chemical Society
The Underestimated Potential of Organometallic Rhenium Complexes as Anticancer Agents
text, January 2014
- Leonidova, Anna; Gasser, Gilles
- American Chemical Society
Works referencing / citing this record:
Intracellular Localization of an Osmocenyl‐Tamoxifen Derivative in Breast Cancer Cells Revealed by Synchrotron Radiation X‐ray Fluorescence Nanoimaging
journal, March 2019
- Fus, Florin; Yang, Yang; Lee, Hui Zhi Shirley
- Angewandte Chemie, Vol. 131, Issue 11
Intracellular Localization of an Osmocenyl‐Tamoxifen Derivative in Breast Cancer Cells Revealed by Synchrotron Radiation X‐ray Fluorescence Nanoimaging
journal, March 2019
- Fus, Florin; Yang, Yang; Lee, Hui Zhi Shirley
- Angewandte Chemie International Edition, Vol. 58, Issue 11
Substituted bidentate and ancillary ligands modulate the bioimaging properties of the classical Re( i ) tricarbonyl core with yeasts and bacteria
journal, January 2017
- Carreño, Alexander; Aros, Alejandra E.; Otero, Carolina
- New Journal of Chemistry, Vol. 41, Issue 5
Exploring the cellular uptake and localisation of phosphorescent rhenium fac -tricarbonyl metallosurfactants as a function of lipophilicity
journal, January 2018
- Hallett, Andrew J.; Placet, Emeline; Prieux, Roxane
- Dalton Transactions, Vol. 47, Issue 40
Nuclear localization of dirhodium( ii ) complexes in breast cancer cells by X-ray fluorescence microscopy
journal, January 2019
- Enriquez Garcia, Alejandra; Lai, Barry; Gopinathan, Sesha Gopal
- Chemical Communications, Vol. 55, Issue 57
Photophysical and biological investigation of phenol substituted rhenium tetrazolato complexes
journal, January 2019
- Akabar, Nurshadrina; Chaturvedi, Vishal; Shillito, Georgina E.
- Dalton Transactions, Vol. 48, Issue 41
Design of Rhenium Compounds in Targeted Anticancer Therapeutics
journal, November 2019
- Collery, Philippe; Desmaele, Didier; Vijaykumar, Veena
- Current Pharmaceutical Design, Vol. 25, Issue 31
Highly Potent rhenium(I) Tricarbonyl Complexes with Dual Anticancer and Anti-Angiogenic Activity Against Colorectal Carcinoma
journal, March 2020
- Delasoie, Joachim; Pavic, Aleksandar; Voutier, Noemie
- Biological and Medicinal Chemistry
Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.