Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase

Donohoe, Bryon S.; Wei, Hui; Mittal, Ashutosh; Shollenberger, Todd; Lunin, Vladimir V.; Himmel, Michael E.; Brunecky, Roman

doi:10.1038/s41598-017-04502-1

Title: Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase

Abstract

In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Furthermore, our results suggest that cellulose β-1–4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.

Authors:

^[1];

^[1]; Mittal, Ashutosh ^[1]; Shollenberger, Todd ^[1]; Lunin, Vladimir V. ^[1]; Himmel, Michael E. ^[1]; Brunecky, Roman ^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)

Publication Date:: Thu Jun 29 00:00:00 EDT 2017

Research Org.:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), NREL Laboratory Directed Research and Development (LDRD)

OSTI Identifier:: 1372043

Report Number(s):: NREL/JA-2700-67215
Journal ID: ISSN 2045-2322

Grant/Contract Number:: AC36-08GO28308

Resource Type:: Accepted Manuscript

Journal Name:: Scientific Reports

Additional Journal Information:: Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; plant cell wall; recalcitrance; AcCel5A endoglucanase; biomass; in planta expression; bioalcohols; molecular engineering in plants

Citation Formats


                    Donohoe, Bryon S., Wei, Hui, Mittal, Ashutosh, Shollenberger, Todd, Lunin, Vladimir V., Himmel, Michael E., and Brunecky, Roman. Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase.  United States: N. p., 2017. 
Web.  doi:10.1038/s41598-017-04502-1.

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                    Donohoe, Bryon S., Wei, Hui, Mittal, Ashutosh, Shollenberger, Todd, Lunin, Vladimir V., Himmel, Michael E., & Brunecky, Roman. Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase.  United States.  https://doi.org/10.1038/s41598-017-04502-1

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                    Donohoe, Bryon S., Wei, Hui, Mittal, Ashutosh, Shollenberger, Todd, Lunin, Vladimir V., Himmel, Michael E., and Brunecky, Roman. Thu .  
"Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase".  United States.  https://doi.org/10.1038/s41598-017-04502-1.  https://www.osti.gov/servlets/purl/1372043.

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@article{osti_1372043,

  title        = {Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase},

  author       = {Donohoe, Bryon S. and Wei, Hui and Mittal, Ashutosh and Shollenberger, Todd and Lunin, Vladimir V. and Himmel, Michael E. and Brunecky, Roman},

  abstractNote = {In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Furthermore, our results suggest that cellulose β-1–4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.},

  doi          = {10.1038/s41598-017-04502-1},

  journal      = {Scientific Reports},

  number       = 1,

  volume       = 7,

  place        = {United States},

  year         = {Thu Jun 29 00:00:00 EDT 2017},

  month        = {Thu Jun 29 00:00:00 EDT 2017}

}

Copy to clipboard

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https://doi.org/10.1038/s41598-017-04502-1

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Works referencing / citing this record:

Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility
journal, August 2018

Xiao, Yao; He, Xuejun; Ojeda-Lassalle, Yemaiza
Biotechnology for Biofuels, Vol. 11, Issue 1
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Title: Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase

Abstract

Citation Formats

Functional co-expression of a fungal ferulic acid esterase and a β-1,4 endoxylanase in Festuca arundinacea (tall fescue) modifies post-harvest cell wall deconstruction journal, April 2015

High level expression of Acidothermus cellulolyticus β-1, 4-endoglucanase in transgenic rice enhances the hydrolysis of its straw by cultured cow gastric fluid journal, January 2011

Functional co-expression of a fungal ferulic acid esterase and a β-1,4 endoxylanase in Festuca arundinacea (tall fescue) modifies post-harvest cell wall deconstruction journal, April 2015

Transgenic ferritin overproduction enhances thermochemical pretreatments in Arabidopsis journal, January 2015

Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose journal, May 2015

Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei journal, January 2015

Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion journal, December 2009

High level expression of Acidothermus cellulolyticus β-1, 4-endoglucanase in transgenic rice enhances the hydrolysis of its straw by cultured cow gastric fluid journal, January 2011

High-pressure freezing for the preservation of biological structure: Theory and practice journal, November 1989

How endogenous plant cell-wall degradation mechanisms can help achieve higher efficiency in saccharification of biomass journal, April 2015

O -Acetylation of plant cell wall polysaccharides: identification and partial characterization of a rhamnogalacturonan O -acetyl-transferase from potato suspension-cultured cells journal, March 2000

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize journal, January 2011

Approaches to achieve high-level heterologous protein production in plants journal, January 2007

Crystal Structure of Thermostable Family 5 Endocellulase E1 from Acidothermus cellulolyticus in Complex with Cellotetraose † , ‡ journal, January 1996

Expression of Glycosyl Hydrolases in Lignocellulosic Feedstock: An Alternative for Affordable Cellulosic Ethanol Production journal, June 2016

Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels journal, August 2008

Expression of Acidothermus cellulolyticus E1 endo-β-1,4-glucanase catalytic domain in transplastomic tobacco journal, August 2009

Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice journal, April 2016

Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei text, January 2015

Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion journal, December 2009

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production journal, February 2007

Transformation of Agrobacterium Using Electroporation journal, December 2006

An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer journal, October 1959

Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility journal, January 2014

A new thermostable endoglucanase,Acidothermus cellulolyticus E1: Synergism withTrichoderma reesei CBH I and comparison to Thermomonospora fusca E5 journal, March 1994

Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant journal, March 2014

Engineering a thermoregulated intein-modified xylanase into maize for consolidated lignocellulosic biomass processing journal, October 2012

How endogenous plant cell-wall degradation mechanisms can help achieve higher efficiency in saccharification of biomass journal, April 2015

Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases journal, August 1989

Quantitation ofAcidothermus cellulolyticus E1 endoglucanase andThermomonospora fusca E3 exoglucanase using enzyme-linked immunosorbent assay (ELISA) journal, September 1995

Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant journal, March 2014

Approaches to achieve high-level heterologous protein production in plants journal, January 2007

Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose journal, May 2015

Crystal Structure of Thermostable Family 5 Endocellulase E1 from Acidothermus cellulolyticus in Complex with Cellotetraose † , ‡ journal, January 1996

Galacturonosyltransferase (GAUT)1 and GAUT7 are the core of a plant cell wall pectin biosynthetic homogalacturonan:galacturonosyltransferase complex journal, November 2011

Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility journal, August 2018

Exploring the Treasure of Plant Molecules With Integrated Biorefineries journal, April 2019

Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa journal, April 2019

Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility journal, August 2018

Engineering hydroxyproline‐ O ‐glycosylated biopolymers to reconstruct the plant cell wall for improved biomass processability journal, January 2020

Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa journal, April 2019

From nano- to micrometer scale: the role of microwave-assisted acid and alkali pretreatments in the sugarcane biomass structure journal, March 2018

Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum) journal, December 2017

Functional co-expression of a fungal ferulic acid esterase and a β-1,4 endoxylanase in Festuca arundinacea (tall fescue) modifies post-harvest cell wall deconstruction
journal, April 2015

High level expression of Acidothermus cellulolyticus β-1, 4-endoglucanase in transgenic rice enhances the hydrolysis of its straw by cultured cow gastric fluid
journal, January 2011

Functional co-expression of a fungal ferulic acid esterase and a β-1,4 endoxylanase in Festuca arundinacea (tall fescue) modifies post-harvest cell wall deconstruction
journal, April 2015

Transgenic ferritin overproduction enhances thermochemical pretreatments in Arabidopsis
journal, January 2015

Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose
journal, May 2015

Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei
journal, January 2015

Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion
journal, December 2009

High level expression of Acidothermus cellulolyticus β-1, 4-endoglucanase in transgenic rice enhances the hydrolysis of its straw by cultured cow gastric fluid
journal, January 2011

High-pressure freezing for the preservation of biological structure: Theory and practice
journal, November 1989

How endogenous plant cell-wall degradation mechanisms can help achieve higher efficiency in saccharification of biomass
journal, April 2015

O -Acetylation of plant cell wall polysaccharides: identification and partial characterization of a rhamnogalacturonan O -acetyl-transferase from potato suspension-cultured cells
journal, March 2000

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
journal, January 2011

Approaches to achieve high-level heterologous protein production in plants
journal, January 2007

Crystal Structure of Thermostable Family 5 Endocellulase E1 from Acidothermus cellulolyticus in Complex with Cellotetraose ^† ^, ^‡
journal, January 1996

Expression of Glycosyl Hydrolases in Lignocellulosic Feedstock: An Alternative for Affordable Cellulosic Ethanol Production
journal, June 2016

Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels
journal, August 2008

Expression of Acidothermus cellulolyticus E1 endo-β-1,4-glucanase catalytic domain in transplastomic tobacco
journal, August 2009

Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice
journal, April 2016

Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei
text, January 2015

Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion
journal, December 2009

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

Transformation of Agrobacterium Using Electroporation
journal, December 2006

An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer
journal, October 1959

Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility
journal, January 2014

A new thermostable endoglucanase,Acidothermus cellulolyticus E1: Synergism withTrichoderma reesei CBH I and comparison to Thermomonospora fusca E₅
journal, March 1994

Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
journal, March 2014

Engineering a thermoregulated intein-modified xylanase into maize for consolidated lignocellulosic biomass processing
journal, October 2012

How endogenous plant cell-wall degradation mechanisms can help achieve higher efficiency in saccharification of biomass
journal, April 2015

Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases
journal, August 1989

Quantitation ofAcidothermus cellulolyticus E1 endoglucanase andThermomonospora fusca E3 exoglucanase using enzyme-linked immunosorbent assay (ELISA)
journal, September 1995

Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
journal, March 2014

Approaches to achieve high-level heterologous protein production in plants
journal, January 2007

Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose
journal, May 2015

Crystal Structure of Thermostable Family 5 Endocellulase E1 from Acidothermus cellulolyticus in Complex with Cellotetraose ^† ^, ^‡
journal, January 1996

Galacturonosyltransferase (GAUT)1 and GAUT7 are the core of a plant cell wall pectin biosynthetic homogalacturonan:galacturonosyltransferase complex
journal, November 2011

Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility
journal, August 2018

Exploring the Treasure of Plant Molecules With Integrated Biorefineries
journal, April 2019

Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa
journal, April 2019

Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility
journal, August 2018

Engineering hydroxyproline‐ O ‐glycosylated biopolymers to reconstruct the plant cell wall for improved biomass processability
journal, January 2020

Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa
journal, April 2019

From nano- to micrometer scale: the role of microwave-assisted acid and alkali pretreatments in the sugarcane biomass structure
journal, March 2018

Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)
journal, December 2017