Recalcitrant carbohydrates after enzymatic hydrolysis of pretreated lignocellulosic biomass
Abstract
To reduce the cost of the enzymes for the hydrolysis of lignocellulosic biomass, two main strategies have been followed: one, the reduction of enzyme dosing by the use of more efficient and stable enzymatic cocktails; another, to include accessory enzymes in the cocktails to increase yields by reducing the recalcitrant carbohydrate fraction remaining at the end of the process. To guide this second strategy, we have explored the chemical bond composition of different fractions of recalcitrant carbohydrates after enzymatic hydrolysis. As a result, two lignocellulosic feedstocks of relevance for the biofuels industry have been analyzed, corn stover and sugarcane straw. On comparing the composition of chemical bonds of the starting pretreated material with samples after standard and forced hydrolysis (with enzyme overdosing), we obtained similar sugar and chemical bond composition. In conclusion, this suggests that the current enzymatic cocktails bear the set of enzymes needed to hydrolyze these feedstocks. From our point of view, the results show the need for a parallel fine-tuning of the enzymatic cocktails with the pretreatment process to maximize sugar release yield.
- Authors:
-
- C/Energia Solar, Seville (Spain)
- Univ. of Georgia, Athens, GA (United States)
- Univ. de Sevilla y CSIC, Seville (Spain)
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1364046
- Grant/Contract Number:
- FG02-93ER20097
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; enzymatic hydrolysis; lignocellulosic biomass; recalcitrant carbohydrates; bioethanol
Citation Formats
Alcantara, Maria Angeles Bermudez, Dobruchowska, Justyna, Azadi, Parastoo, Garcia, Bruno Diez, Molina-Heredia, Fernando P., and Reyes-Sosa, Francisco Manuel. Recalcitrant carbohydrates after enzymatic hydrolysis of pretreated lignocellulosic biomass. United States: N. p., 2016.
Web. doi:10.1186/s13068-016-0629-4.
Alcantara, Maria Angeles Bermudez, Dobruchowska, Justyna, Azadi, Parastoo, Garcia, Bruno Diez, Molina-Heredia, Fernando P., & Reyes-Sosa, Francisco Manuel. Recalcitrant carbohydrates after enzymatic hydrolysis of pretreated lignocellulosic biomass. United States. https://doi.org/10.1186/s13068-016-0629-4
Alcantara, Maria Angeles Bermudez, Dobruchowska, Justyna, Azadi, Parastoo, Garcia, Bruno Diez, Molina-Heredia, Fernando P., and Reyes-Sosa, Francisco Manuel. Thu .
"Recalcitrant carbohydrates after enzymatic hydrolysis of pretreated lignocellulosic biomass". United States. https://doi.org/10.1186/s13068-016-0629-4. https://www.osti.gov/servlets/purl/1364046.
@article{osti_1364046,
title = {Recalcitrant carbohydrates after enzymatic hydrolysis of pretreated lignocellulosic biomass},
author = {Alcantara, Maria Angeles Bermudez and Dobruchowska, Justyna and Azadi, Parastoo and Garcia, Bruno Diez and Molina-Heredia, Fernando P. and Reyes-Sosa, Francisco Manuel},
abstractNote = {To reduce the cost of the enzymes for the hydrolysis of lignocellulosic biomass, two main strategies have been followed: one, the reduction of enzyme dosing by the use of more efficient and stable enzymatic cocktails; another, to include accessory enzymes in the cocktails to increase yields by reducing the recalcitrant carbohydrate fraction remaining at the end of the process. To guide this second strategy, we have explored the chemical bond composition of different fractions of recalcitrant carbohydrates after enzymatic hydrolysis. As a result, two lignocellulosic feedstocks of relevance for the biofuels industry have been analyzed, corn stover and sugarcane straw. On comparing the composition of chemical bonds of the starting pretreated material with samples after standard and forced hydrolysis (with enzyme overdosing), we obtained similar sugar and chemical bond composition. In conclusion, this suggests that the current enzymatic cocktails bear the set of enzymes needed to hydrolyze these feedstocks. From our point of view, the results show the need for a parallel fine-tuning of the enzymatic cocktails with the pretreatment process to maximize sugar release yield.},
doi = {10.1186/s13068-016-0629-4},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {United States},
year = {Thu Oct 06 00:00:00 EDT 2016},
month = {Thu Oct 06 00:00:00 EDT 2016}
}
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journal, August 1984
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Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 2: morphological and structural substrate analysis
journal, January 2014
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- Biotechnology for Biofuels, Vol. 7, Issue 1
RESEARCH: Development of a mature fungal technology and production platform for industrial enzymes based on a Myceliophthora thermophila isolate, previously known as Chrysosporium lucknowense C1
journal, June 2011
- Visser, Hans; Joosten, Vivi; Punt, Peter J.
- Industrial Biotechnology, Vol. 7, Issue 3
Coupling NMR to NOM
journal, March 2004
- Cook, Robert L.
- Analytical and Bioanalytical Chemistry, Vol. 378, Issue 6
Quantitative solid-state 13
C NMR spectroscopy of organic matter fractions in lowland rice soils
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journal, January 2011
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- Biotechnology for Biofuels, Vol. 4, Issue 1
Carbon-13 nuclear magnetic resonance spectroscopy of [1-13C] enriched monosaccharides. Signal assignments and orientational dependence of geminal and vicinal carbon-carbon and carbon-hydrogen spin-spin coupling constants
journal, September 1976
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- Journal of the American Chemical Society, Vol. 98, Issue 19
Characterisation of thermally modified hard- and softwoods by C CPMAS NMR
journal, December 2004
- Wikberg, H.; Liisamaunu, S.
- Carbohydrate Polymers, Vol. 58, Issue 4
Works referencing / citing this record:
Management of enzyme diversity in high-performance cellulolytic cocktails
journal, June 2017
- Reyes-Sosa, Francisco Manuel; López Morales, Macarena; Platero Gómez, Ana Isabel
- Biotechnology for Biofuels, Vol. 10, Issue 1