Calcium and zinc differentially affect the structure of lipid membranes
Abstract
Interactions of calcium (Ca2+) and zinc (Zn2+) cations with biomimetic membranes made of dipalmitoylphosphatidylcholine (DPPC) were studied by small angle neutron diffraction (SAND). Experiments show that the structure of these lipid bilayers is differentially affected by the two divalent cations. Initially, both Ca2+ and Zn2+ cause DPPC bilayers to thicken, while further increases in Ca2+ concentration result in the bilayer thinning, eventually reverting to having the same thickness as pure DPPC. The binding of Zn2+, on the other hand, causes the bilayers to swell to a maximum thickness, and the addition of more Zn2+ does not result in a further thickening of the membrane. Agreement between our results obtained using oriented planar membranes and those from vesicular samples implies that the effect of cations on bilayer thickness is the result of electrostatic interactions, rather than geometrical constraints due to bilayer curvature. This notion is further reinforced by MD simulations. Lastly, the radial distribution functions reveal a strong interaction between Ca2+ and the phosphate oxygens, while Zn2+ shows a much weaker binding specificity.
- Authors:
-
[1];
- Comenius Univ. in Bratislava, Bratislava (Slovakia); Joint Institute for Nuclear Research, Dubna - Moscow Region (Russia)
- Joint Institute for Nuclear Research, Dubna - Moscow Region (Russia)
- Joint Institute for Nuclear Research, Dubna - Moscow Region (Russia); Dubna State Univ., Dubna - Moscow Region (Russia)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
- Comenius Univ. in Bratislava, Bratislava (Slovakia)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1351793
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Langmuir
- Additional Journal Information:
- Journal Volume: 33; Journal Issue: 12; Journal ID: ISSN 0743-7463
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Kučerka, Norbert, Dushanov, Ermuhammad, Kholmurodov, Kholmirzo T., Katsaras, John, and Uhríková, Daniela. Calcium and zinc differentially affect the structure of lipid membranes. United States: N. p., 2017.
Web. doi:10.1021/acs.langmuir.6b03228.
Kučerka, Norbert, Dushanov, Ermuhammad, Kholmurodov, Kholmirzo T., Katsaras, John, & Uhríková, Daniela. Calcium and zinc differentially affect the structure of lipid membranes. United States. https://doi.org/10.1021/acs.langmuir.6b03228
Kučerka, Norbert, Dushanov, Ermuhammad, Kholmurodov, Kholmirzo T., Katsaras, John, and Uhríková, Daniela. Thu .
"Calcium and zinc differentially affect the structure of lipid membranes". United States. https://doi.org/10.1021/acs.langmuir.6b03228. https://www.osti.gov/servlets/purl/1351793.
@article{osti_1351793,
title = {Calcium and zinc differentially affect the structure of lipid membranes},
author = {Kučerka, Norbert and Dushanov, Ermuhammad and Kholmurodov, Kholmirzo T. and Katsaras, John and Uhríková, Daniela},
abstractNote = {Interactions of calcium (Ca2+) and zinc (Zn2+) cations with biomimetic membranes made of dipalmitoylphosphatidylcholine (DPPC) were studied by small angle neutron diffraction (SAND). Experiments show that the structure of these lipid bilayers is differentially affected by the two divalent cations. Initially, both Ca2+ and Zn2+ cause DPPC bilayers to thicken, while further increases in Ca2+ concentration result in the bilayer thinning, eventually reverting to having the same thickness as pure DPPC. The binding of Zn2+, on the other hand, causes the bilayers to swell to a maximum thickness, and the addition of more Zn2+ does not result in a further thickening of the membrane. Agreement between our results obtained using oriented planar membranes and those from vesicular samples implies that the effect of cations on bilayer thickness is the result of electrostatic interactions, rather than geometrical constraints due to bilayer curvature. This notion is further reinforced by MD simulations. Lastly, the radial distribution functions reveal a strong interaction between Ca2+ and the phosphate oxygens, while Zn2+ shows a much weaker binding specificity.},
doi = {10.1021/acs.langmuir.6b03228},
journal = {Langmuir},
number = 12,
volume = 33,
place = {United States},
year = {Thu Mar 09 00:00:00 EST 2017},
month = {Thu Mar 09 00:00:00 EST 2017}
}
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