An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis
Abstract
The molecular basis of enzyme catalytic power and specificity derives from dynamic interactions between enzyme and substrate during catalysis. While considerable effort has been devoted to understanding how conformational dynamics within enzymes affect catalysis, the role of conformational dynamics within protein substrates has not been addressed. Here in this paper, we examine the importance of substrate dynamics in the cleavage of Kunitz-BPTI protease inhibitors by mesotrypsin, finding that the varied conformational dynamics of structurally similar substrates can profoundly impact the rate of catalysis. A 1.4 Å crystal structure of a mesotrypsin-product complex formed with a rapidly cleaved substrate reveals a dramatic conformational change in the substrate upon proteolysis. Using long all-atom molecular dynamics simulations of acyl-enzyme intermediates with proteolysis rates spanning three orders of magnitude, we identify global and local dynamic features of substrates on the ns-μs timescale that correlate with enzymatic rates and explain differential susceptibility to proteolysis. By integrating multiple enhanced sampling methods for molecular dynamics, we model a viable conformational pathway between substratelike and product-like states, linking substrate dynamics on the ns-μs timescale with large collective substrate motions on the much slower timescale of catalysis. Our findings implicate substrate flexibility as a critical determinant of catalysis.
- Authors:
-
- Mayo Clinic, Jacksonville, Florida (United States). College of Medicine, Dept. of Cancer Biology
- Ben-Gurion Univ. of the Negev, Beer-Sheva (Israel). Dept. of Biotechnology Engineering and the National Inst. of Biotechnology
- Brookhaven National Lab. (BNL), Upton, NY (United States). Photon Sciences Directorate
- Mayo Clinic, Jacksonville, Florida (United States). College of Medicine, Dept. of Neuroscience
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1351724
- Report Number(s):
- BNL-113701-2017-JA
Journal ID: ISSN 0021-9258
- Grant/Contract Number:
- SC0012704
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Biological Chemistry
- Additional Journal Information:
- Journal Volume: 291; Journal Issue: 51; Journal ID: ISSN 0021-9258
- Publisher:
- American Society for Biochemistry and Molecular Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Protein dynamic; protein conformation; serine protease; protease inhibitor; proteolysis; enzyme catalysis; protein structure; crystal structure; molecular dynamics; molecular modeling
Citation Formats
Kayode, Olumide, Wang, Ruiying, Pendlebury, Devon F., Cohen, Itay, Henin, Rachel D., Hockla, Alexandra, Soares, Alexei S., Papo, Niv, Caulfield, Thomas R., and Radisky, Evette S. An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis. United States: N. p., 2016.
Web. doi:10.1074/jbc.M116.758417.
Kayode, Olumide, Wang, Ruiying, Pendlebury, Devon F., Cohen, Itay, Henin, Rachel D., Hockla, Alexandra, Soares, Alexei S., Papo, Niv, Caulfield, Thomas R., & Radisky, Evette S. An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis. United States. https://doi.org/10.1074/jbc.M116.758417
Kayode, Olumide, Wang, Ruiying, Pendlebury, Devon F., Cohen, Itay, Henin, Rachel D., Hockla, Alexandra, Soares, Alexei S., Papo, Niv, Caulfield, Thomas R., and Radisky, Evette S. Thu .
"An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis". United States. https://doi.org/10.1074/jbc.M116.758417. https://www.osti.gov/servlets/purl/1351724.
@article{osti_1351724,
title = {An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis},
author = {Kayode, Olumide and Wang, Ruiying and Pendlebury, Devon F. and Cohen, Itay and Henin, Rachel D. and Hockla, Alexandra and Soares, Alexei S. and Papo, Niv and Caulfield, Thomas R. and Radisky, Evette S.},
abstractNote = {The molecular basis of enzyme catalytic power and specificity derives from dynamic interactions between enzyme and substrate during catalysis. While considerable effort has been devoted to understanding how conformational dynamics within enzymes affect catalysis, the role of conformational dynamics within protein substrates has not been addressed. Here in this paper, we examine the importance of substrate dynamics in the cleavage of Kunitz-BPTI protease inhibitors by mesotrypsin, finding that the varied conformational dynamics of structurally similar substrates can profoundly impact the rate of catalysis. A 1.4 Å crystal structure of a mesotrypsin-product complex formed with a rapidly cleaved substrate reveals a dramatic conformational change in the substrate upon proteolysis. Using long all-atom molecular dynamics simulations of acyl-enzyme intermediates with proteolysis rates spanning three orders of magnitude, we identify global and local dynamic features of substrates on the ns-μs timescale that correlate with enzymatic rates and explain differential susceptibility to proteolysis. By integrating multiple enhanced sampling methods for molecular dynamics, we model a viable conformational pathway between substratelike and product-like states, linking substrate dynamics on the ns-μs timescale with large collective substrate motions on the much slower timescale of catalysis. Our findings implicate substrate flexibility as a critical determinant of catalysis.},
doi = {10.1074/jbc.M116.758417},
journal = {Journal of Biological Chemistry},
number = 51,
volume = 291,
place = {United States},
year = {Thu Nov 03 00:00:00 EDT 2016},
month = {Thu Nov 03 00:00:00 EDT 2016}
}
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