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Title: Micro-differential scanning calorimeter for liquid biological samples

Abstract

Here, we developed an ultrasensitive micro-DSC (differential scanning calorimeter) for liquid protein sample characterization. Our design integrated vanadium oxide thermistors and flexible polymer substrates with microfluidics chambers to achieve a high sensitivity (6 V/W), low thermal conductivity (0.7 mW/K), high power resolutions (40 nW), and well-defined liquid volume (1 μl) calorimeter sensor in a compact and cost-effective way. Furthermore, we demonstrated the performance of the sensor with lysozyme unfolding. The measured transition temperature and enthalpy change were in accordance with the previous literature data. This micro-DSC could potentially raise the prospect of high-throughput biochemical measurement by parallel operation with miniaturized sample consumption.

Authors:
ORCiD logo [1];  [2];  [3]; ORCiD logo [4];  [4];  [5];  [2]
+ Show Author Affiliations
  1. Stony Brook Univ., NY (United States). Dept. of Mechanical Engineering
  2. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Mechanical Engineering
  3. AbbVie, Deutschland (Germany)
  4. AbbVie Bioresearch Center, Worcester, MA (United States)
  5. Brookhaven National Lab. (BNL), Upton, NY (United States). Center for Functional Nanomaterials
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States). Center for Functional Nanomaterials (CFN)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1349571
Report Number(s):
BNL-113700-2017-JA
Journal ID: ISSN 0034-6748; KC0403020; TRN: US1701353
Grant/Contract Number:  
SC00112704; AC02-98CH10886
Resource Type:
Accepted Manuscript
Journal Name:
Review of Scientific Instruments
Additional Journal Information:
Journal Volume: 87; Journal Issue: 10; Journal ID: ISSN 0034-6748
Publisher:
American Institute of Physics (AIP)
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 42 ENGINEERING; 59 BASIC BIOLOGICAL SCIENCES; Thermistors; BioMEMS; Microfluidics; Proteins; Polymers; Center for Functional Nanomaterials

Citation Formats

Wang, Shuyu, Yu, Shifeng, Siedler, Michael S., Ihnat, Peter M., Filoti, Dana I., Lu, Ming, and Zuo, Lei. Micro-differential scanning calorimeter for liquid biological samples. United States: N. p., 2016. Web. doi:10.1063/1.4965443.
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Wang, Shuyu, Yu, Shifeng, Siedler, Michael S., Ihnat, Peter M., Filoti, Dana I., Lu, Ming, & Zuo, Lei. Micro-differential scanning calorimeter for liquid biological samples. United States. https://doi.org/10.1063/1.4965443
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Wang, Shuyu, Yu, Shifeng, Siedler, Michael S., Ihnat, Peter M., Filoti, Dana I., Lu, Ming, and Zuo, Lei. Thu . "Micro-differential scanning calorimeter for liquid biological samples". United States. https://doi.org/10.1063/1.4965443. https://www.osti.gov/servlets/purl/1349571.
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@article{osti_1349571,
title = {Micro-differential scanning calorimeter for liquid biological samples},
author = {Wang, Shuyu and Yu, Shifeng and Siedler, Michael S. and Ihnat, Peter M. and Filoti, Dana I. and Lu, Ming and Zuo, Lei},
abstractNote = {Here, we developed an ultrasensitive micro-DSC (differential scanning calorimeter) for liquid protein sample characterization. Our design integrated vanadium oxide thermistors and flexible polymer substrates with microfluidics chambers to achieve a high sensitivity (6 V/W), low thermal conductivity (0.7 mW/K), high power resolutions (40 nW), and well-defined liquid volume (1 μl) calorimeter sensor in a compact and cost-effective way. Furthermore, we demonstrated the performance of the sensor with lysozyme unfolding. The measured transition temperature and enthalpy change were in accordance with the previous literature data. This micro-DSC could potentially raise the prospect of high-throughput biochemical measurement by parallel operation with miniaturized sample consumption.},
doi = {10.1063/1.4965443},
journal = {Review of Scientific Instruments},
number = 10,
volume = 87,
place = {United States},
year = {Thu Oct 20 00:00:00 EDT 2016},
month = {Thu Oct 20 00:00:00 EDT 2016}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1063/1.4965443
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Works referenced in this record:

Enthalpy arrays
journal, June 2004

  • Torres, F. E.; Kuhn, P.; De Bruyker, D.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 26
  • DOI: 10.1073/pnas.0403573101

Nano-calorimetry of small-sized biological samples
journal, October 2008

Electrospray-assisted nanocalorimetry measurements
journal, October 2013

Measurement and analysis of results obtained on biological substances with d.s.c.
journal, November 2001

  • Hinz, Hans-Jürgen; Schwarz, Frederick P.
  • The Journal of Chemical Thermodynamics, Vol. 33, Issue 11
  • DOI: 10.1006/jcht.2001.0861

Thermal conductivity of single biological cells and relation with cell viability
journal, May 2013

  • Kyoo Park, Byoung; Yi, Namwoo; Park, Jaesung
  • Applied Physics Letters, Vol. 102, Issue 20
  • DOI: 10.1063/1.4807471

Demonstration of MEMS-based differential scanning calorimetry for determining thermodynamic properties of biomolecules
journal, September 2008

Higher throughput calorimetry: opportunities, approaches and challenges
journal, October 2010

  • Torres, Francisco E.; Recht, Michael I.; Coyle, Joseph E.
  • Current Opinion in Structural Biology, Vol. 20, Issue 5
  • DOI: 10.1016/j.sbi.2010.09.001

Scanning calorimeter for nanoliter-scale liquid samples
journal, October 2000

  • Olson, E. A.; Efremov, M. Yu.; Kwan, A. T.
  • Applied Physics Letters, Vol. 77, Issue 17
  • DOI: 10.1063/1.1319506

Chip-calorimetric monitoring of biofilm eradication with antibiotics provides mechanistic information
journal, April 2013

  • Mariana, Frida; Buchholz, Friederike; Lerchner, Johannes
  • International Journal of Medical Microbiology, Vol. 303, Issue 3
  • DOI: 10.1016/j.ijmm.2012.12.009

Enthalpy array analysis of enzymatic and binding reactions
journal, June 2008

  • Recht, Michael I.; Bruyker, Dirk De; Bell, Alan G.
  • Analytical Biochemistry, Vol. 377, Issue 1
  • DOI: 10.1016/j.ab.2008.03.007

Isothermal Titration Calorimetry in Nanoliter Droplets with Subsecond Time Constants
journal, October 2011

  • Lubbers, Brad; Baudenbacher, Franz
  • Analytical Chemistry, Vol. 83, Issue 20
  • DOI: 10.1021/ac202026a

MEMS Scanning Calorimeter With Serpentine-Shaped Platinum Resistors for Characterizations of Microsamples
journal, April 2009

  • Youssef, S.; Podlecki, J.; Al Asmar, R.
  • Journal of Microelectromechanical Systems, Vol. 18, Issue 2
  • DOI: 10.1109/jmems.2009.2013392

Highly sensitive ac nanocalorimeter for microliter-scale liquids or biological samples
journal, May 2004

  • Garden, J. -L.; Château, E.; Chaussy, J.
  • Applied Physics Letters, Vol. 84, Issue 18
  • DOI: 10.1063/1.1737794

A polymer-based MEMS differential scanning calorimeter
journal, July 2015

High-sensitivity microfluidic calorimeters for biological and chemical applications
journal, August 2009

  • Lee, W.; Fon, W.; Axelrod, B. W.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 36
  • DOI: 10.1073/pnas.0901447106

Highly sensitive parylene membrane-based ac-calorimeter for small mass magnetic samples
journal, May 2010

  • Lopeandia, A. F.; André, E.; Garden, J. -L.
  • Review of Scientific Instruments, Vol. 81, Issue 5
  • DOI: 10.1063/1.3422247

Phase separation in polymer blend thin films studied by differential AC chip calorimetry
journal, February 2010

ac nanocalorimeter for measuring heat capacity of biological macromolecules in solution
journal, September 2003

  • Yao, Haruhiko; Ema, Kenji; Fukada, Harumi
  • Review of Scientific Instruments, Vol. 74, Issue 9
  • DOI: 10.1063/1.1602958

Temperature-modulated differential scanning calorimetry in a MEMS device
journal, April 2013

Monitoring protein denaturation using thermal conductivity probe
journal, January 2013

Calorimeter chip calibration for thermal characterization of liquid samples
journal, August 2009

Calorimetric biosensors with integrated microfluidic channels
journal, July 2004

  • Zhang, Yuyan; Tadigadapa, Srinivas
  • Biosensors and Bioelectronics, Vol. 19, Issue 12, p. 1733-1743
  • DOI: 10.1016/j.bios.2004.01.009

A polymer-based MEMS differential scanning calorimeter
conference, January 2014

  • Jia, Yuan; Wang, Bin; Zhu, Jing
  • 2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS)
  • DOI: 10.1109/memsys.2014.6765637
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    Works referencing / citing this record:

    Microfabrication-based isothermal titration calorimetry using a combined in-mixing and post-mixing titration approach
    journal, January 2018

    • Feng, Xiangsong; Jia, Yuan; Jiang, Hongyuan
    • Analytical Methods, Vol. 10, Issue 38
    • DOI: 10.1039/c8ay00402a

    Nanocalorimetry: Exploring materials faster and smaller
    journal, September 2019

    • Yi, Feng; LaVan, David A.
    • Applied Physics Reviews, Vol. 6, Issue 3
    • DOI: 10.1063/1.5098297

    Nanocalorimeters for biomolecular analysis and cell metabolism monitoring
    journal, January 2020

    • Wang, Shuyu; Sha, Xiaopeng; Yu, Shifeng
    • Biomicrofluidics, Vol. 14, Issue 1
    • DOI: 10.1063/1.5134870
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