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Title: Rapid and continuous magnetic separation in droplet microfluidic devices

Abstract

Here, we present a droplet microfluidic method to extract molecules of interest from a droplet in a rapid and continuous fashion. We accomplish this by first marginalizing functionalized super-paramagnetic beads within the droplet using a magnetic field, and then splitting the droplet into one droplet containing the majority of magnetic beads and one droplet containing the minority fraction. We quantitatively analysed the factors which affect the efficiency of marginalization and droplet splitting to optimize the enrichment of magnetic beads. We first characterized the interplay between the droplet velocity and the strength of the magnetic field and its effect on marginalization. We found that marginalization is optimal at the midline of the magnet and that marginalization is a good predictor of bead enrichment through splitting at low to moderate droplet velocities. Finally, we focused our efforts on manipulating the splitting profile to improve the enrichment provided by asymmetric splitting. We designed asymmetric splitting forks that employ capillary effects to preferentially extract the bead-rich regions of the droplets. Our strategy represents a framework to optimize magnetic bead enrichment methods tailored to the requirements of specific droplet-based applications. We anticipate that our separation technology is well suited for applications in single-cell genomics andmore » proteomics. In particular, our method could be used to separate mRNA bound to poly-dT functionalized magnetic microparticles from single cell lysates to prepare single-cell cDNA libraries.« less

Authors:
 [1];  [1];  [1];  [1]
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  1. Stony Brook Univ., Stony Brook, NY (United States)
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1346969
Grant/Contract Number:  
AC02-98CH10886
Resource Type:
Accepted Manuscript
Journal Name:
Lab on a chip (Print)
Additional Journal Information:
Journal Name: Lab on a chip (Print); Journal Volume: 15; Journal Issue: 3; Journal ID: ISSN 1473-0197
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS; 60 APPLIED LIFE SCIENCES

Citation Formats

Brouzes, Eric, Kruse, Travis, Kimmerling, Robert, and Strey, Helmut H. Rapid and continuous magnetic separation in droplet microfluidic devices. United States: N. p., 2014. Web. doi:10.1039/c4lc01327a.
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Brouzes, Eric, Kruse, Travis, Kimmerling, Robert, & Strey, Helmut H. Rapid and continuous magnetic separation in droplet microfluidic devices. United States. https://doi.org/10.1039/c4lc01327a
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Brouzes, Eric, Kruse, Travis, Kimmerling, Robert, and Strey, Helmut H. Wed . "Rapid and continuous magnetic separation in droplet microfluidic devices". United States. https://doi.org/10.1039/c4lc01327a. https://www.osti.gov/servlets/purl/1346969.
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@article{osti_1346969,
title = {Rapid and continuous magnetic separation in droplet microfluidic devices},
author = {Brouzes, Eric and Kruse, Travis and Kimmerling, Robert and Strey, Helmut H.},
abstractNote = {Here, we present a droplet microfluidic method to extract molecules of interest from a droplet in a rapid and continuous fashion. We accomplish this by first marginalizing functionalized super-paramagnetic beads within the droplet using a magnetic field, and then splitting the droplet into one droplet containing the majority of magnetic beads and one droplet containing the minority fraction. We quantitatively analysed the factors which affect the efficiency of marginalization and droplet splitting to optimize the enrichment of magnetic beads. We first characterized the interplay between the droplet velocity and the strength of the magnetic field and its effect on marginalization. We found that marginalization is optimal at the midline of the magnet and that marginalization is a good predictor of bead enrichment through splitting at low to moderate droplet velocities. Finally, we focused our efforts on manipulating the splitting profile to improve the enrichment provided by asymmetric splitting. We designed asymmetric splitting forks that employ capillary effects to preferentially extract the bead-rich regions of the droplets. Our strategy represents a framework to optimize magnetic bead enrichment methods tailored to the requirements of specific droplet-based applications. We anticipate that our separation technology is well suited for applications in single-cell genomics and proteomics. In particular, our method could be used to separate mRNA bound to poly-dT functionalized magnetic microparticles from single cell lysates to prepare single-cell cDNA libraries.},
doi = {10.1039/c4lc01327a},
journal = {Lab on a chip (Print)},
number = 3,
volume = 15,
place = {United States},
year = {Wed Dec 03 00:00:00 EST 2014},
month = {Wed Dec 03 00:00:00 EST 2014}
}
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https://doi.org/10.1039/c4lc01327a
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