Toward production of jet fuel functionality in oilseeds: Identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds
Abstract
Seeds of members of the genus Cuphea accumulate medium-chain fatty acids (MCFAs; 8:0–14:0). MCFA- and palmitic acid- (16:0) rich vegetable oils have received attention for jet fuel production, given their similarity in chain length to Jet A fuel hydrocarbons. Studies were conducted to test genes, including those from Cuphea, for their ability to confer jet fuel-type fatty acid accumulation in seed oil of the emerging biofuel crop Camelina sativa. Transcriptomes from Cuphea viscosissima and Cuphea pulcherrima developing seeds that accumulate >90% of C8 and C10 fatty acids revealed three FatB cDNAs (CpuFatB3, CvFatB1, and CpuFatB4) expressed predominantly in seeds and structurally divergent from typical FatB thioesterases that release 16:0 from acyl carrier protein (ACP). Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0. Co-expression of combinations of previously characterized Cuphea and California bay FatBs produced Camelina oils with mixtures of C8–C16 fatty acids, but amounts of each fatty acid were less than obtained by expression of individual FatB cDNAs. Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconutmore »
- Authors:
-
- Univ. of Nebraska-Lincoln, Lincoln, NE (United States)
- Indiana Univ., Bloomington, IN (United States)
- Donald Danforth Plant Science Center, Saint Louis, MO (United States)
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Center for Advanced Biofuel Systems (CABS); Univ. of of Nebraska-Lincoln, Lincoln, NE (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1343284
- Grant/Contract Number:
- SC0001295
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Experimental Botany
- Additional Journal Information:
- Journal Volume: 66; Journal Issue: 14; Journal ID: ISSN 0022-0957
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; Camelina; Cuphea; FatB acyl-ACP thioesterase; jet fuel oilseed; medium-chain fatty acid
Citation Formats
Kim, Hae Jin, Silva, Jillian E., Vu, Hieu Sy, Mockaitis, Keithanne, Nam, Jeong -Won, and Cahoon, Edgar B. Toward production of jet fuel functionality in oilseeds: Identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds. United States: N. p., 2015.
Web. doi:10.1093/jxb/erv225.
Kim, Hae Jin, Silva, Jillian E., Vu, Hieu Sy, Mockaitis, Keithanne, Nam, Jeong -Won, & Cahoon, Edgar B. Toward production of jet fuel functionality in oilseeds: Identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds. United States. https://doi.org/10.1093/jxb/erv225
Kim, Hae Jin, Silva, Jillian E., Vu, Hieu Sy, Mockaitis, Keithanne, Nam, Jeong -Won, and Cahoon, Edgar B. Mon .
"Toward production of jet fuel functionality in oilseeds: Identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds". United States. https://doi.org/10.1093/jxb/erv225. https://www.osti.gov/servlets/purl/1343284.
@article{osti_1343284,
title = {Toward production of jet fuel functionality in oilseeds: Identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds},
author = {Kim, Hae Jin and Silva, Jillian E. and Vu, Hieu Sy and Mockaitis, Keithanne and Nam, Jeong -Won and Cahoon, Edgar B.},
abstractNote = {Seeds of members of the genus Cuphea accumulate medium-chain fatty acids (MCFAs; 8:0–14:0). MCFA- and palmitic acid- (16:0) rich vegetable oils have received attention for jet fuel production, given their similarity in chain length to Jet A fuel hydrocarbons. Studies were conducted to test genes, including those from Cuphea, for their ability to confer jet fuel-type fatty acid accumulation in seed oil of the emerging biofuel crop Camelina sativa. Transcriptomes from Cuphea viscosissima and Cuphea pulcherrima developing seeds that accumulate >90% of C8 and C10 fatty acids revealed three FatB cDNAs (CpuFatB3, CvFatB1, and CpuFatB4) expressed predominantly in seeds and structurally divergent from typical FatB thioesterases that release 16:0 from acyl carrier protein (ACP). Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0. Co-expression of combinations of previously characterized Cuphea and California bay FatBs produced Camelina oils with mixtures of C8–C16 fatty acids, but amounts of each fatty acid were less than obtained by expression of individual FatB cDNAs. Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs. RNA interference (RNAi) suppression of Camelina β-ketoacyl-ACP synthase II, however, reduced 12:0 in seeds expressing a 12:0-ACP-specific FatB. Here, Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.},
doi = {10.1093/jxb/erv225},
journal = {Journal of Experimental Botany},
number = 14,
volume = 66,
place = {United States},
year = {Mon May 11 00:00:00 EDT 2015},
month = {Mon May 11 00:00:00 EDT 2015}
}
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Works referenced in this record:
Substrates of diacylglycerol acyltransferase in microsomes from developing oil seeds
journal, June 1994
- Wiberg, Eva; Tillberg, Elisabeth; Stymne, Sten
- Phytochemistry, Vol. 36, Issue 3
A specific acyl-ACP thioesterase implicated in medium-chain fatty acid production in immature cotyledons of Umbellularia californica
journal, February 1991
- Pollard, Michael R.; Anderson, Lana; Fan, Calvin
- Archives of Biochemistry and Biophysics, Vol. 284, Issue 2, p. 306-312
Identification of acyltransferases controlling triacylglycerol biosynthesis in oilseeds using a genomics-based approach
journal, April 2005
- Roscoe, Thomas J.
- European Journal of Lipid Science and Technology, Vol. 107, Issue 4
Camelina: An Emerging Oilseed Platform for Advanced Biofuels and Bio-Based Materials
book, November 2013
- Iskandarov, Umidjon; Kim, Hae Jin; Cahoon, Edgar B.
- Plants and BioEnergy, p. 131-140
Trends in industrial uses of palm and lauric oils
journal, February 1985
- Knaut, J.; Richtler, H. J.
- Journal of the American Oil Chemists' Society, Vol. 62, Issue 2
Conjugated fatty acids accumulate to high levels in phospholipids of metabolically engineered soybean and Arabidopsis seeds
journal, June 2006
- Cahoon, Edgar B.; Dietrich, Charles R.; Meyer, Knut
- Phytochemistry, Vol. 67, Issue 12
Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases.
journal, March 1995
- Jones, A.; Davies, H. M.; Voelker, T. A.
- The Plant Cell, Vol. 7, Issue 3
Genetic and biochemical basis for alternative routes of tocotrienol biosynthesis for enhanced vitamin E antioxidant production
journal, December 2012
- Zhang, Chunyu; Cahoon, Rebecca E.; Hunter, Sarah C.
- The Plant Journal, Vol. 73, Issue 4
Investigating the Unique Properties of Cuphea-Derived Biodiesel Fuel with the Advanced Distillation Curve Method
journal, June 2010
- Lovestead, Tara M.; Windom, Bret C.; Bruno, Thomas J.
- Energy & Fuels, Vol. 24, Issue 6
Camelina seed transcriptome: a tool for meal and oil improvement and translational research
journal, April 2013
- Nguyen, Huu T.; Silva, Jillian E.; Podicheti, Ram
- Plant Biotechnology Journal, Vol. 11, Issue 6
Renewable jet fuel
journal, April 2014
- Kallio, Pauli; Pásztor, András; Akhtar, M. Kalim
- Current Opinion in Biotechnology, Vol. 26
Metabolism of exogenous long-chain fatty acids by spinach leaves
journal, December 1987
- Roughan, P. Grattan; Thompson, Guy A.; Cho, Sung Ho
- Archives of Biochemistry and Biophysics, Vol. 259, Issue 2, p. 481-496
Acyltransferase action in the modification of seed oil biosynthesis
journal, October 2009
- Snyder, Crystal L.; Yurchenko, Olga P.; Siloto, Rodrigo M. P.
- New Biotechnology, Vol. 26, Issue 1-2
Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil
journal, October 2008
- Burgal, Julie; Shockey, Jay; Lu, Chaofu
- Plant Biotechnology Journal, Vol. 6, Issue 8
A Cuphea β‐ketoacyl‐ACP synthase shifts the synthesis of fatty acids towards shorter chains in Arabidopsis seeds expressing Cuphea FatB thioesterases
journal, March 1998
- Leonard, Jeffrey M.; Knapp, Steven J.; Slabaugh, Mary B.
- The Plant Journal, Vol. 13, Issue 5
Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity
journal, January 2011
- Jing, Fuyuan; Cantu, David C.; Tvaruzkova, Jarmila
- BMC Biochemistry, Vol. 12, Issue 1
Ubiquitous and Endoplasmic Reticulum–Located Lysophosphatidyl Acyltransferase, LPAT2, Is Essential for Female but Not Male Gametophyte Development in Arabidopsis
journal, March 2005
- Kim, Hyun Uk; Li, Yubing; Huang, Anthony H. C.
- The Plant Cell, Vol. 17, Issue 4
Acyl-Lipid Metabolism
journal, January 2013
- Li-Beisson, Yonghua; Shorrosh, Basil; Beisson, Fred
- The Arabidopsis Book, Vol. 11
Identification of a potential bottleneck in branched chain fatty acid incorporation into triacylglycerol for lipid biosynthesis in agronomic plants
journal, June 2009
- Nlandu Mputu, M.; Rhazi, L.; Vasseur, G.
- Biochimie, Vol. 91, Issue 6
Palmitoyl-Acyl Carrier Protein (ACP) Thioesterase and the Evolutionary Origin of Plant Acyl-ACP Thioesterases
journal, March 1995
- Jones, Aubrey; Davies, H. Maelor; Voelker, Toni A.
- The Plant Cell, Vol. 7, Issue 3
Substrate specificities of glycerol acylating enzymes from developing embryos of two Cuphea species
journal, September 1992
- Bafor, Maureen; Stymne, Sten
- Phytochemistry, Vol. 31, Issue 9
Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants
journal, July 1992
- Voelker, T.; Worrell, A.; Anderson, L.
- Science, Vol. 257, Issue 5066
Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants
journal, July 1992
- Voelker, T.; Worrell, A.; Anderson, L.
- Science, Vol. 257, Issue 5066
Lysophosphatidic Acid Acyltransferase from Coconut Endosperm Mediates the Insertion of Laurate at the sn-2 Position of Triacylglycerols in Lauric Rapeseed Oil and Can Increase Total Laurate Levels
journal, July 1999
- Knutzon, Deborah S.; Hayes, Thomas R.; Wyrick, Annette
- Plant Physiology, Vol. 120, Issue 3
Condensing enzymes from Cuphea wrightii associated with medium chain fatty acid biosynthesis
journal, March 1998
- Slabaugh, Mary B.; Leonard, Jeffrey M.; Knapp, Steven J.
- The Plant Journal, Vol. 13, Issue 5
Production of high levels of 8:0 and 10:0 fatty acids in transgenic canola by overexpression of Ch FatB2, a thioesterase cDNA from Cuphea hookeriana
journal, February 1996
- Dehesh, Katayoon; Jones, Aubrey; Knutzon, Deborah S.
- The Plant Journal, Vol. 9, Issue 2, p. 167-172
Fatty acid synthesis is inhibited by inefficient utilization of unusual fatty acids for glycerolipid assembly
journal, January 2014
- Bates, P. D.; Johnson, S. R.; Cao, X.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 3
Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux
journal, June 2007
- Cahoon, Edgar B.; Shockey, Jay M.; Dietrich, Charles R.
- Current Opinion in Plant Biology, Vol. 10, Issue 3
Ubiquitous and Endoplasmic Reticulum–Located Lysophosphatidyl Acyltransferase, LPAT2, Is Essential for Female but Not Male Gametophyte Development in Arabidopsis
journal, March 2005
- Kim, Hyun Uk; Li, Yubing; Huang, Anthony H. C.
- The Plant Cell, Vol. 17, Issue 4
A simple and efficient method for isolating RNA from pine trees
journal, June 1993
- Chang, Shujun; Puryear, Jeff; Cairney, John
- Plant Molecular Biology Reporter, Vol. 11, Issue 2
Camelina seed transcriptome: a tool for meal and oil improvement and translational research
journal, April 2013
- Nguyen, Huu T.; Silva, Jillian E.; Podicheti, Ram
- Plant Biotechnology Journal, Vol. 11, Issue 6
The glycinin Gy 1 gene from soybean
journal, January 1989
- Sims, Thomas L.; Goldberg, Robert B.
- Nucleic Acids Research, Vol. 17, Issue 11
High-value oils from plants
journal, May 2008
- Dyer, John M.; Stymne, Sten; Green, Allan G.
- The Plant Journal, Vol. 54, Issue 4
A Rapid Method of Total Lipid Extraction and Purification
journal, January 1959
- Bligh, E. G.; Dyer, W. J.
- Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 1
Medium-chain fatty acid biosynthesis and utilization in Brassica napus plants expressing lauroyl-acyl carrier protein thioesterase
journal, January 1996
- Eccleston, VictoriaS.; Cranmer, AnnM.; Voelker, ToniA.
- Planta, Vol. 198, Issue 1
Imaging heterogeneity of membrane and storage lipids in transgenic Camelina sativa seeds with altered fatty acid profiles
journal, July 2013
- Horn, Patrick J.; Silva, Jillian E.; Anderson, Danielle
- The Plant Journal
Transformation of the oilseed crop Camelina sativa by Agrobacterium-mediated floral dip and simple large-scale screening of transformants
journal, September 2012
- Liu, Xunjia; Brost, Jennifer; Hutcheon, Carolyn
- In Vitro Cellular & Developmental Biology - Plant, Vol. 48, Issue 5
New FATB thioesterases from a high-laurateCuphea species: Functional and complementation analyses
journal, December 2006
- Filichkin, Sergei A.; Slabaugh, Mary B.; Knapp, Steven J.
- European Journal of Lipid Science and Technology, Vol. 108, Issue 12
Fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time PCR assay
journal, January 2013
- Mieog, Jos C.; Howitt, Crispin A.; Ral, Jean-Philippe
- BMC Plant Biology, Vol. 13, Issue 1
Plant Acyl-ACP Thioesterases: Chain-Length Determining Enzymes in Plant Fatty Acid Biosynthesis
book, January 1996
- Voelker, Toni
- Genetic Engineering
Redirection of metabolic flux for high levels of omega-7 monounsaturated fatty acid accumulation in camelina seeds
journal, July 2014
- Nguyen, Huu Tam; Park, Hyunwoo; Koster, Karen L.
- Plant Biotechnology Journal, Vol. 13, Issue 1
Identification of a potential bottleneck in branched chain fatty acid incorporation into triacylglycerol for lipid biosynthesis in agronomic plants
journal, June 2009
- Nlandu Mputu, M.; Rhazi, L.; Vasseur, G.
- Biochimie, Vol. 91, Issue 6
New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications
journal, April 2011
- Lu, Chaofu; Napier, Johnathan A.; Clemente, Thomas E.
- Current Opinion in Biotechnology, Vol. 22, Issue 2
A specific acyl-ACP thioesterase implicated in medium-chain fatty acid production in immature cotyledons of Umbellularia californica
journal, February 1991
- Pollard, Michael R.; Anderson, Lana; Fan, Calvin
- Archives of Biochemistry and Biophysics, Vol. 284, Issue 2, p. 306-312
Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity
journal, January 2011
- Jing, Fuyuan; Cantu, David C.; Tvaruzkova, Jarmila
- BMC Biochemistry, Vol. 12, Issue 1
Genetic transformation and gene silencing mediated by multiple copies of a transgene in eastern white pine
journal, December 2006
- Tang, W.; Newton, R. J.; Weidner, D. A.
- Journal of Experimental Botany, Vol. 58, Issue 3
RNA isolation from siliques, dry seeds, and other tissues of Arabidopsis thaliana
journal, October 2004
- Suzuki, Yuji; Kawazu, Tetsu; Koyama, Hiroyuki
- BioTechniques, Vol. 37, Issue 4
New FATB thioesterases from a high-laurateCuphea species: Functional and complementation analyses
journal, December 2006
- Filichkin, Sergei A.; Slabaugh, Mary B.; Knapp, Steven J.
- European Journal of Lipid Science and Technology, Vol. 108, Issue 12
Acyl-Lipid Metabolism
journal, January 2013
- Li-Beisson, Yonghua; Shorrosh, Basil; Beisson, Fred
- The Arabidopsis Book, Vol. 11
Renewable jet fuel
journal, April 2014
- Kallio, Pauli; Pásztor, András; Akhtar, M. Kalim
- Current Opinion in Biotechnology, Vol. 26
Identification of a plastid acyl-acyl carrier protein synthetase in Arabidopsis and its role in the activation and elongation of exogenous fatty acids
journal, October 2005
- Koo, Abraham J. K.; Fulda, Martin; Browse, John
- The Plant Journal, Vol. 44, Issue 4, p. 620-632
The glycinin Gy 1 gene from soybean
journal, January 1989
- Sims, Thomas L.; Goldberg, Robert B.
- Nucleic Acids Research, Vol. 17, Issue 11
A Rapid Method of Total Lipid Extraction and Purification
journal, January 1959
- Bligh, E. G.; Dyer, W. J.
- Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 1
Camelina (Camelina sativa (L.) Crantz): agronomic potential in Mediterranean environments and diversity for biofuel and food uses
journal, January 2013
- Campbell, M. C.; Rossi, A. F.; Erskine, W.
- Crop and Pasture Science, Vol. 64, Issue 4
The distribution of caprylate, caprate and laurate in lipids from developing and mature seeds of transgenic Brassica napus L.
journal, December 2000
- Wiberg, Eva; Edwards, Patricia; Byrne, James
- Planta, Vol. 212, Issue 1
Genetic and biochemical basis for alternative routes of tocotrienol biosynthesis for enhanced vitamin E antioxidant production
journal, December 2012
- Zhang, Chunyu; Cahoon, Rebecca E.; Hunter, Sarah C.
- The Plant Journal, Vol. 73, Issue 4
Two Novel Thioesterases Are Key Determinants of the Bimodal Distribution of Acyl Chain Length of Cuphea palustris Seed Oil
journal, January 1996
- Dehesh, K.; Edwards, P.; Hayes, T.
- Plant Physiology, Vol. 110, Issue 1
How can we genetically engineer oilseed crops to produce high levels of medium-chain fatty acids?
journal, October 2001
- Dehesh, Katayoon
- European Journal of Lipid Science and Technology, Vol. 103, Issue 10
Cuphea Oil as a Potential Biodiesel Feedstock to Improve Fuel Properties
journal, September 2014
- Knothe, Gerhard
- Journal of Energy Engineering, Vol. 140, Issue 3
Substrate specificities of glycerol acylating enzymes from developing embryos of two Cuphea species
journal, September 1992
- Bafor, Maureen; Stymne, Sten
- Phytochemistry, Vol. 31, Issue 9
Total seed oil and fatty acid methyl ester contents of Cuphea accessions
journal, July 2006
- Phippen, Winthrop B.; Isbell, Terry A.; Phippen, Mary E.
- Industrial Crops and Products, Vol. 24, Issue 1
Induction, purification and characterisation of acyl-ACP thioesterase from developing seeds of oil seed rape (Brassica napus)
journal, December 1992
- Hellyer, Amanda; Leadlay, Peter F.; Slabas, Antoni R.
- Plant Molecular Biology, Vol. 20, Issue 5
Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis
journal, February 2013
- Tjellström, Henrik; Strawsine, Merissa; Silva, Jillian
- FEBS Letters, Vol. 587, Issue 7
Modulating seed β-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil
journal, March 2007
- Pidkowich, M. S.; Nguyen, H. T.; Heilmann, I.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 11, p. 4742-4747
Lysophosphatidic Acid Acyltransferase from Coconut Endosperm Mediates the Insertion of Laurate at the sn-2 Position of Triacylglycerols in Lauric Rapeseed Oil and Can Increase Total Laurate Levels
journal, July 1999
- Knutzon, Deborah S.; Hayes, Thomas R.; Wyrick, Annette
- Plant Physiology, Vol. 120, Issue 3
The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure
journal, April 2014
- Kagale, Sateesh; Koh, Chushin; Nixon, John
- Nature Communications, Vol. 5, Issue 1
Genetic engineering of a quantitative trait: metabolic and genetic parameters influencing the accumulation of laurate in rapeseed
journal, February 1996
- Voelker, Toni A.; Hayes, Thomas R.; Cranmer, Ann M.
- The Plant Journal, Vol. 9, Issue 2
The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure
journal, April 2014
- Kagale, Sateesh; Koh, Chushin; Nixon, John
- Nature Communications, Vol. 5, Issue 1
Composition of seed oils in some Latin American Cuphea (Lythraceae)
journal, September 1992
- Graham, Shirley A.; Kleiman, Robert
- Industrial Crops and Products, Vol. 1, Issue 1
Metabolism of exogenous long-chain fatty acids by spinach leaves
journal, December 1987
- Roughan, P. Grattan; Thompson, Guy A.; Cho, Sung Ho
- Archives of Biochemistry and Biophysics, Vol. 259, Issue 2, p. 481-496
Metabolic Engineering of Fatty Acid Biosynthesis in Plants
journal, January 2002
- Thelen, Jay J.; Ohlrogge, John B.
- Metabolic Engineering, Vol. 4, Issue 1
Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil
journal, October 2008
- Burgal, Julie; Shockey, Jay; Lu, Chaofu
- Plant Biotechnology Journal, Vol. 6, Issue 8
Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation
journal, September 2007
- Lu, Chaofu; Kang, Jinling
- Plant Cell Reports, Vol. 27, Issue 2
Genetic transformation and gene silencing mediated by multiple copies of a transgene in eastern white pine
journal, December 2006
- Tang, W.; Newton, R. J.; Weidner, D. A.
- Journal of Experimental Botany, Vol. 58, Issue 3
Revision of Cuphea Section Diploptychia (Lythraceae)
journal, January 1998
- Graham, Shirley A.
- Systematic Botany Monographs, Vol. 53
New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications
journal, April 2011
- Lu, Chaofu; Napier, Johnathan A.; Clemente, Thomas E.
- Current Opinion in Biotechnology, Vol. 22, Issue 2
A Rapid Method of Total Lipid Extraction and Purification
journal, August 1959
- Bligh, E. G.; Dyer, W. J.
- Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 8
MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
journal, October 2013
- Tamura, Koichiro; Stecher, Glen; Peterson, Daniel
- Molecular Biology and Evolution, Vol. 30, Issue 12
Acyltransferase action in the modification of seed oil biosynthesis
journal, October 2009
- Snyder, Crystal L.; Yurchenko, Olga P.; Siloto, Rodrigo M. P.
- New Biotechnology, Vol. 26, Issue 1-2
Cuphea Oil as Source of Biodiesel with Improved Fuel Properties Caused by High Content of Methyl Decanoate †
journal, March 2009
- Knothe, Gerhard; Cermak, Steven C.; Evangelista, Roque L.
- Energy & Fuels, Vol. 23, Issue 3
Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux
journal, June 2007
- Cahoon, Edgar B.; Shockey, Jay M.; Dietrich, Charles R.
- Current Opinion in Plant Biology, Vol. 10, Issue 3
Fuel properties of oil from genetically altered Cuphea viscosissima
journal, January 1999
- Geller, Daniel P.; Goodrum, John W.; Knapp, Steven J.
- Industrial Crops and Products, Vol. 9, Issue 2
Purification and characterization of the stearoyl-acyl carrier protein desaturase and the acyl-acyl carrier protein thioesterase from maturing seeds of safflower.
journal, October 1982
- McKeon, T. A.; Stumpf, P. K.
- Journal of Biological Chemistry, Vol. 257, Issue 20
Works referencing / citing this record:
Identification of Genes Involved in Lipid Biosynthesis through de novo Transcriptome Assembly from Cocos nucifera Developing Endosperm
journal, January 2019
- Reynolds, Kyle B.; Cullerne, Darren P.; El Tahchy, Anna
- Plant and Cell Physiology, Vol. 60, Issue 5
Palmitvaccenic Acid (Δ11- cis -hexadecenoic acid) Is Synthesized by an OLE1-like Desaturase in the Arbuscular Mycorrhiza Fungus Rhizophagus irregularis
journal, March 2020
- Brands, Mathias; Cahoon, Edgar B.; Dörmann, Peter
- Biochemistry, Vol. 59, Issue 11
Synthetic redesign of plant lipid metabolism
journal, June 2016
- Haslam, Richard P.; Sayanova, Olga; Kim, Hae Jin
- The Plant Journal, Vol. 87, Issue 1
Identification of Genes Involved in Lipid Biosynthesis through de novo Transcriptome Assembly from Cocos nucifera Developing Endosperm
journal, January 2019
- Reynolds, Kyle B.; Cullerne, Darren P.; El Tahchy, Anna
- Plant and Cell Physiology, Vol. 60, Issue 5
Synthetic redesign of plant lipid metabolism
journal, June 2016
- Haslam, Richard P.; Sayanova, Olga; Kim, Hae Jin
- The Plant Journal, Vol. 87, Issue 1
Camelina, a Swiss knife for plant lipid biotechnology
journal, June 2016
- Faure, Jean-Denis; Tepfer, Mark
- OCL, Vol. 23, Issue 5
Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids
journal, July 2018
- Bansal, Sunil; Kim, Hae Jin; Na, GunNam
- Journal of Experimental Botany, Vol. 69, Issue 18
Structurally divergent lysophosphatidic acid acyltransferases with high selectivity for saturated medium chain fatty acids from Cuphea seeds
journal, December 2015
- Kim, Hae Jin; Silva, Jillian E.; Iskandarov, Umidjon
- The Plant Journal, Vol. 84, Issue 5
Harnessing Biotechnology for the Development of New Seed Lipid Traits in Brassica
journal, May 2019
- Zhou, Xue-Rong; Li, Jun; Wan, Xia
- Plant and Cell Physiology, Vol. 60, Issue 6
Simultaneous Targeting of Multiple Gene Homeologs to Alter Seed Oil Production in Camelina sativa
journal, April 2017
- Aznar-Moreno, J. A.; Durrett, T. P.
- Plant and Cell Physiology, Vol. 58, Issue 7
Discontinuous fatty acid elongation yields hydroxylated seed oil with improved function
journal, August 2018
- Li, Xiangjun; Teitgen, Alicen M.; Shirani, Asghar
- Nature Plants, Vol. 4, Issue 9
A reconfigured Kennedy pathway which promotes efficient accumulation of medium-chain fatty acids in leaf oils
journal, May 2017
- Reynolds, Kyle B.; Taylor, Matthew C.; Cullerne, Darren P.
- Plant Biotechnology Journal, Vol. 15, Issue 11
How did nature engineer the highest surface lipid accumulation among plants? Exceptional expression of acyl-lipid-associated genes for the assembly of extracellular triacylglycerol by Bayberry (Myrica pensylvanica) fruits
journal, September 2016
- Simpson, Jeffrey P.; Thrower, Nicholas; Ohlrogge, John B.
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1861, Issue 9
Tailoring the composition of novel wax esters in the seeds of transgenic Camelina sativa through systematic metabolic engineering
journal, February 2017
- Ruiz-Lopez, Noemi; Broughton, Richard; Usher, Sarah
- Plant Biotechnology Journal, Vol. 15, Issue 7
Accumulation of medium-chain, saturated fatty acyl moieties in seed oils of transgenic Camelina sativa
journal, February 2017
- Hu, Zhaohui; Wu, Qian; Dalal, Jyoti
- PLOS ONE, Vol. 12, Issue 2
Accumulation of medium-chain, saturated fatty acyl moieties in seed oils of transgenic Camelina sativa
journal, February 2017
- Hu, Zhaohui; Wu, Qian; Dalal, Jyoti
- PLOS ONE, Vol. 12, Issue 2
Stepwise metabolic engineering of Escherichia coli to produce triacylglycerol rich in medium-chain fatty acids
journal, June 2018
- Xu, Lin; Wang, Lian; Zhou, Xue-Rong
- Biotechnology for Biofuels, Vol. 11, Issue 1
A reconfigured Kennedy pathway which promotes efficient accumulation of medium-chain fatty acids in leaf oils
journal, May 2017
- Reynolds, Kyle B.; Taylor, Matthew C.; Cullerne, Darren P.
- Plant Biotechnology Journal, Vol. 15, Issue 11
Stepwise metabolic engineering of Escherichia coli to produce triacylglycerol rich in medium-chain fatty acids
journal, June 2018
- Xu, Lin; Wang, Lian; Zhou, Xue-Rong
- Biotechnology for Biofuels, Vol. 11, Issue 1
Camelina sativa, an oilseed at the nexus between model system and commercial crop
journal, June 2018
- Malik, Meghna R.; Tang, Jihong; Sharma, Nirmala
- Plant Cell Reports, Vol. 37, Issue 10
Metabolic Engineering for Enhanced Medium Chain Omega Hydroxy Fatty Acid Production in Escherichia coli
journal, February 2018
- Xiao, Kang; Yue, Xiu-Hong; Chen, Wen-Chao
- Frontiers in Microbiology, Vol. 9