A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides
Abstract
We characterized the effect of gold and aluminum zero-mode waveguides (ZMWs) on the brightness of immobilized single emitters by probing fluorophores that absorb in the green and red regions of the visible spectrum. Aluminum ZMWs enhance the emission of Atto565 fluorophores upon green excitation, but they do not enhance the emission of Atto647N fluorophores upon red excitation. Gold ZMWs increase emission of both fluorophores with Atto647N showing enhancement that is threefold higher than that observed for Atto565. Our work indicates that 200 nm gold ZMWs are better suited for single-molecule fluorescence studies in the red region of the visible spectrum, while aluminum appears more suited for the green region of the visible spectrum.
- Authors:
-
- Univ. of Kentucky, Lexington, KY (United States). Dept. of Chemistry
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Material Science
- Univ. of Copenhagen (Denmark). Nano-science Center and Dept. of Chemistry
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1342673
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Physical Chemistry. A, Molecules, Spectroscopy, Kinetics, Environment, and General Theory
- Additional Journal Information:
- Journal Volume: 120; Journal Issue: 34; Journal ID: ISSN 1089-5639
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Martin, William Elliott, Srijanto, Bernadeta R., Collier, C. Patrick, Vosch, Tom, and Richards, Christopher I. A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides. United States: N. p., 2016.
Web. doi:10.1021/acs.jpca.6b03309.
Martin, William Elliott, Srijanto, Bernadeta R., Collier, C. Patrick, Vosch, Tom, & Richards, Christopher I. A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides. United States. https://doi.org/10.1021/acs.jpca.6b03309
Martin, William Elliott, Srijanto, Bernadeta R., Collier, C. Patrick, Vosch, Tom, and Richards, Christopher I. Sat .
"A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides". United States. https://doi.org/10.1021/acs.jpca.6b03309. https://www.osti.gov/servlets/purl/1342673.
@article{osti_1342673,
title = {A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides},
author = {Martin, William Elliott and Srijanto, Bernadeta R. and Collier, C. Patrick and Vosch, Tom and Richards, Christopher I.},
abstractNote = {We characterized the effect of gold and aluminum zero-mode waveguides (ZMWs) on the brightness of immobilized single emitters by probing fluorophores that absorb in the green and red regions of the visible spectrum. Aluminum ZMWs enhance the emission of Atto565 fluorophores upon green excitation, but they do not enhance the emission of Atto647N fluorophores upon red excitation. Gold ZMWs increase emission of both fluorophores with Atto647N showing enhancement that is threefold higher than that observed for Atto565. Our work indicates that 200 nm gold ZMWs are better suited for single-molecule fluorescence studies in the red region of the visible spectrum, while aluminum appears more suited for the green region of the visible spectrum.},
doi = {10.1021/acs.jpca.6b03309},
journal = {Journal of Physical Chemistry. A, Molecules, Spectroscopy, Kinetics, Environment, and General Theory},
number = 34,
volume = 120,
place = {United States},
year = {Sat Aug 06 00:00:00 EDT 2016},
month = {Sat Aug 06 00:00:00 EDT 2016}
}
Web of Science
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