A purple cupredoxin from Nitrosopumilus maritimus containing a mononuclear type 1 copper center with an open binding site
Abstract
Mononuclear cupredoxin proteins usually contain a coordinately saturated type 1 copper (T1Cu) center and function exclusively as electron carriers. Here we report a cupredoxin isolated from the nitrifying archaeon Nitrosopumilus maritimus SCM1, called Nmar1307, that contains a T1Cu center with an open binding site containing water. It displays a deep purple color due to strong absorptions around 413 nm (1880 M–1 cm–1) and 558 nm (2290 M–1 cm–1) in the UV–vis electronic spectrum. EPR studies suggest the protein contains two Cu(II) species of nearly equal population, one nearly axial, with hyperfine constant A∥ = 98 × 10–4 cm–1, and another more rhombic, with a smaller A∥ value of 69 × 10–4 cm–1. The X-ray crystal structure at 1.6 Å resolution confirms that it contains a Cu atom coordinated by two His and one Cys in a trigonal plane, with an axial H2O at 2.25 Å. Both UV–vis absorption and EPR spectroscopic studies suggest that the Nmar1307 can oxidize NO to nitrite, an activity that is attributable to the high reduction potential (354 mV vs SHE) of the copper site. Lastly, these results suggest that mononuclear cupredoxins can have a wide range of structural features, including an open binding site containingmore »
- Authors:
-
- Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Univ. of Washington, Seattle, WA (United States)
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1340348
- Report Number(s):
- BNL-112067-2016-JA
Journal ID: ISSN 0002-7863
- Grant/Contract Number:
- SC00112704
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of the American Chemical Society
- Additional Journal Information:
- Journal Volume: 138; Journal Issue: 20; Journal ID: ISSN 0002-7863
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Hosseinzadeh, Parisa, Tian, Shiliang, Marshall, Nicholas M., Hemp, James, Mullen, Timothy, Nilges, Mark J., Gao, Yi -Gui, Robinson, Howard, Stahl, David A., Gennis, Robert B., and Lu, Yi. A purple cupredoxin from Nitrosopumilus maritimus containing a mononuclear type 1 copper center with an open binding site. United States: N. p., 2016.
Web. doi:10.1021/jacs.5b13128.
Hosseinzadeh, Parisa, Tian, Shiliang, Marshall, Nicholas M., Hemp, James, Mullen, Timothy, Nilges, Mark J., Gao, Yi -Gui, Robinson, Howard, Stahl, David A., Gennis, Robert B., & Lu, Yi. A purple cupredoxin from Nitrosopumilus maritimus containing a mononuclear type 1 copper center with an open binding site. United States. https://doi.org/10.1021/jacs.5b13128
Hosseinzadeh, Parisa, Tian, Shiliang, Marshall, Nicholas M., Hemp, James, Mullen, Timothy, Nilges, Mark J., Gao, Yi -Gui, Robinson, Howard, Stahl, David A., Gennis, Robert B., and Lu, Yi. Wed .
"A purple cupredoxin from Nitrosopumilus maritimus containing a mononuclear type 1 copper center with an open binding site". United States. https://doi.org/10.1021/jacs.5b13128. https://www.osti.gov/servlets/purl/1340348.
@article{osti_1340348,
title = {A purple cupredoxin from Nitrosopumilus maritimus containing a mononuclear type 1 copper center with an open binding site},
author = {Hosseinzadeh, Parisa and Tian, Shiliang and Marshall, Nicholas M. and Hemp, James and Mullen, Timothy and Nilges, Mark J. and Gao, Yi -Gui and Robinson, Howard and Stahl, David A. and Gennis, Robert B. and Lu, Yi},
abstractNote = {Mononuclear cupredoxin proteins usually contain a coordinately saturated type 1 copper (T1Cu) center and function exclusively as electron carriers. Here we report a cupredoxin isolated from the nitrifying archaeon Nitrosopumilus maritimus SCM1, called Nmar1307, that contains a T1Cu center with an open binding site containing water. It displays a deep purple color due to strong absorptions around 413 nm (1880 M–1 cm–1) and 558 nm (2290 M–1 cm–1) in the UV–vis electronic spectrum. EPR studies suggest the protein contains two Cu(II) species of nearly equal population, one nearly axial, with hyperfine constant A∥ = 98 × 10–4 cm–1, and another more rhombic, with a smaller A∥ value of 69 × 10–4 cm–1. The X-ray crystal structure at 1.6 Å resolution confirms that it contains a Cu atom coordinated by two His and one Cys in a trigonal plane, with an axial H2O at 2.25 Å. Both UV–vis absorption and EPR spectroscopic studies suggest that the Nmar1307 can oxidize NO to nitrite, an activity that is attributable to the high reduction potential (354 mV vs SHE) of the copper site. Lastly, these results suggest that mononuclear cupredoxins can have a wide range of structural features, including an open binding site containing water, making this class of proteins even more versatile.},
doi = {10.1021/jacs.5b13128},
journal = {Journal of the American Chemical Society},
number = 20,
volume = 138,
place = {United States},
year = {Wed Apr 27 00:00:00 EDT 2016},
month = {Wed Apr 27 00:00:00 EDT 2016}
}
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Works referencing / citing this record:
Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta
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Accumulation of NO 2 -cobalamin in nutrient-stressed ammonia-oxidizing archaea and in the oxygen deficient zone of the eastern tropical North Pacific: NO 2 -cobalamin in marine communities and isolates
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Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta
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