Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Reversible S-nitrosylation in an engineered azurin

Abstract

Here, S-Nitrosothiols are known as reagents for NO storage and transportation and as regulators in many physiological processes. Although the S-nitrosylation catalysed by haem proteins is well known, no direct evidence of S-nitrosylation in copper proteins has been reported. Here, we report reversible insertion of NO into a copper–thiolate bond in an engineered copper centre in Pseudomonas aeruginosa azurin by rational design of the primary coordination sphere and tuning its reduction potential by deleting a hydrogen bond in the secondary coordination sphere. The results not only provide the first direct evidence of S-nitrosylation of Cu(II)-bound cysteine in metalloproteins, but also shed light on the reaction mechanism and structural features responsible for stabilizing the elusive Cu(I)–S(Cys)NO species. The fast, efficient and reversible S-nitrosylation reaction is used to demonstrate its ability to prevent NO inhibition of cytochrome bo3 oxidase activity by competing for NO binding with the native enzyme under physiologically relevant conditions.

Authors:
 [1];  [1];  [2];  [1];  [1]; ORCiD logo [1];  [3];  [1];  [4];  [2];  [1]
+ Show Author Affiliations
  1. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
  2. Stanford Univ., Stanford, CA (United States)
  3. Brookhaven National Lab. (BNL), Upton, NY (United States)
  4. Oregon Health & Sciences Univ., Portland, OR (United States)
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1340343
Report Number(s):
BNL-112002-2016-JA
Journal ID: ISSN 1755-4330
Grant/Contract Number:  
SC00112704
Resource Type:
Accepted Manuscript
Journal Name:
Nature Chemistry
Additional Journal Information:
Journal Volume: 8; Journal Issue: 7; Journal ID: ISSN 1755-4330
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Tian, Shiliang, Liu, Jing, Cowley, Ryan E., Hosseinzadeh, Parisa, Marshall, Nicholas M., Yu, Yang, Robinson, Howard, Nilges, Mark J., Blackburn, Ninian J., Solomon, Edward I., and Lu, Yi. Reversible S-nitrosylation in an engineered azurin. United States: N. p., 2016. Web. doi:10.1038/nchem.2489.
Copy to clipboard
Tian, Shiliang, Liu, Jing, Cowley, Ryan E., Hosseinzadeh, Parisa, Marshall, Nicholas M., Yu, Yang, Robinson, Howard, Nilges, Mark J., Blackburn, Ninian J., Solomon, Edward I., & Lu, Yi. Reversible S-nitrosylation in an engineered azurin. United States. https://doi.org/10.1038/nchem.2489
Copy to clipboard
Tian, Shiliang, Liu, Jing, Cowley, Ryan E., Hosseinzadeh, Parisa, Marshall, Nicholas M., Yu, Yang, Robinson, Howard, Nilges, Mark J., Blackburn, Ninian J., Solomon, Edward I., and Lu, Yi. Mon . "Reversible S-nitrosylation in an engineered azurin". United States. https://doi.org/10.1038/nchem.2489. https://www.osti.gov/servlets/purl/1340343.
Copy to clipboard
@article{osti_1340343,
title = {Reversible S-nitrosylation in an engineered azurin},
author = {Tian, Shiliang and Liu, Jing and Cowley, Ryan E. and Hosseinzadeh, Parisa and Marshall, Nicholas M. and Yu, Yang and Robinson, Howard and Nilges, Mark J. and Blackburn, Ninian J. and Solomon, Edward I. and Lu, Yi},
abstractNote = {Here, S-Nitrosothiols are known as reagents for NO storage and transportation and as regulators in many physiological processes. Although the S-nitrosylation catalysed by haem proteins is well known, no direct evidence of S-nitrosylation in copper proteins has been reported. Here, we report reversible insertion of NO into a copper–thiolate bond in an engineered copper centre in Pseudomonas aeruginosa azurin by rational design of the primary coordination sphere and tuning its reduction potential by deleting a hydrogen bond in the secondary coordination sphere. The results not only provide the first direct evidence of S-nitrosylation of Cu(II)-bound cysteine in metalloproteins, but also shed light on the reaction mechanism and structural features responsible for stabilizing the elusive Cu(I)–S(Cys)NO species. The fast, efficient and reversible S-nitrosylation reaction is used to demonstrate its ability to prevent NO inhibition of cytochrome bo3 oxidase activity by competing for NO binding with the native enzyme under physiologically relevant conditions.},
doi = {10.1038/nchem.2489},
journal = {Nature Chemistry},
number = 7,
volume = 8,
place = {United States},
year = {Mon Apr 25 00:00:00 EDT 2016},
month = {Mon Apr 25 00:00:00 EDT 2016}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1038/nchem.2489
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 33 works
Citation information provided by
Web of Science
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

CHEMICAL PHYSIOLOGY OF BLOOD FLOW REGULATION BY RED BLOOD CELLS:: The Role of Nitric Oxide and S-Nitrosohemoglobin
journal, March 2005

Activation of the Cardiac Calcium Release Channel (Ryanodine Receptor) by Poly-S-Nitrosylation
journal, January 1998

S-Nitrosylation of β-Arrestin Regulates β-Adrenergic Receptor Trafficking
journal, August 2008

Regulated Protein Denitrosylation by Cytosolic and Mitochondrial Thioredoxins
journal, May 2008

Enzymatic mechanisms regulating protein S-nitrosylation: implications in health and disease
journal, February 2012

Mechanisms of S-nitrosothiol formation and selectivity in nitric oxide signaling
journal, December 2012

Dinitrosyliron complexes and the mechanism(s) of cellular protein nitrosothiol formation from nitric oxide
journal, March 2009

  • Bosworth, C. A.; Toledo, J. C.; Zmijewski, J. W.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 12
  • DOI: 10.1073/pnas.0710416106

The oxyhemoglobin reaction of nitric oxide
journal, August 1999

  • Gow, A. J.; Luchsinger, B. P.; Pawloski, J. R.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 16
  • DOI: 10.1073/pnas.96.16.9027

Crystallographic Analysis of the Interaction of Nitric Oxide with Quaternary-T Human Hemoglobin † , ‡
journal, January 2004

  • Chan, Nei-Li; Kavanaugh, Jeffrey S.; Rogers, Paul H.
  • Biochemistry, Vol. 43, Issue 1
  • DOI: 10.1021/bi030172j

Heme-assisted S-nitrosation of a proximal thiolate in a nitric oxide transport protein
journal, January 2005

  • Weichsel, A.; Maes, E. M.; Andersen, J. F.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 3
  • DOI: 10.1073/pnas.0406549102

Superoxide Dismutase Targets NO from GSNO to Cysβ93 of Oxyhemoglobin in Concentrated but Not Dilute Solutions of the Protein
journal, November 2003

  • Romeo, Andrea A.; Capobianco, John A.; English, Ann M.
  • Journal of the American Chemical Society, Vol. 125, Issue 47
  • DOI: 10.1021/ja0289752

Nitrosothiol Formation Catalyzed by Ceruloplasmin: IMPLICATION FOR CYTOPROTECTIVE MECHANISM IN VIVO
journal, September 1999

  • Inoue, Katsuhisa; Akaike, Takaaki; Miyamoto, Yoichi
  • Journal of Biological Chemistry, Vol. 274, Issue 38
  • DOI: 10.1074/jbc.274.38.27069

Ceruloplasmin is a NO oxidase and nitrite synthase that determines endocrine NO homeostasis
journal, August 2006

  • Shiva, Sruti; Wang, Xunde; Ringwood, Lorna A.
  • Nature Chemical Biology, Vol. 2, Issue 9
  • DOI: 10.1038/nchembio813

Mechanism of S -Nitrosothiol Formation and Degradation Mediated by Copper Ions
journal, October 1999

  • Stubauer, Gottfried; Giuffrè, Alessandro; Sarti, Paolo
  • Journal of Biological Chemistry, Vol. 274, Issue 40
  • DOI: 10.1074/jbc.274.40.28128

Cellular copper management—a draft user's guide
journal, March 2010

  • Bertini, Ivano; Cavallaro, Gabriele; McGreevy, Kathleen S.
  • Coordination Chemistry Reviews, Vol. 254, Issue 5-6
  • DOI: 10.1016/j.ccr.2009.07.024

The mechanism of nitric oxide formation from S-nitrosothiols (thionitrites)
journal, January 1996

The Chemistry of S-Nitrosothiols
journal, October 1999

  • Williams, D. Lyn H.
  • Accounts of Chemical Research, Vol. 32, Issue 10
  • DOI: 10.1021/ar9800439

The reaction of nitric oxide with ceruloplasmin
journal, April 1973

  • Wever, R.; Van Leeuwen, F. X. R.; Van Gelder, B. F.
  • Biochimica et Biophysica Acta (BBA) - Enzymology, Vol. 302, Issue 2
  • DOI: 10.1016/0005-2744(73)90152-6

The interaction of nitric oxide with ascorbate oxidase
journal, October 1975

  • van Leeuwen, F. X. R.; Wever, R.; van Gelder, B. F.
  • Biochimica et Biophysica Acta (BBA) - Enzymology, Vol. 403, Issue 2
  • DOI: 10.1016/0005-2744(75)90058-3

The reaction of nitric oxide with copper proteins and the photodissociation of copper-NO complexes
journal, November 1987

  • Gorren, A. C. F.; de Boer, E.; Wever, R.
  • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 916, Issue 1
  • DOI: 10.1016/0167-4838(87)90208-1

Ligand Binding and Protein Dynamics in Cupredoxins
journal, September 1995

  • Ehrenstein, David; Filiaci, Mattia; Scharf, Birgit
  • Biochemistry, Vol. 34, Issue 38
  • DOI: 10.1021/bi00038a010

What is the real physiological NO concentration in vivo?
journal, September 2009

Type-zero copper proteins
journal, November 2009

  • Lancaster, Kyle M.; George, Serena DeBeer; Yokoyama, Keiko
  • Nature Chemistry, Vol. 1, Issue 9
  • DOI: 10.1038/nchem.412

Outer-Sphere Contributions to the Electronic Structure of Type Zero Copper Proteins
journal, April 2012

  • Lancaster, Kyle M.; Zaballa, María-Eugenia; Sproules, Stephen
  • Journal of the American Chemical Society, Vol. 134, Issue 19
  • DOI: 10.1021/ja302190r

Copper-sulfenate complex from oxidation of a cavity mutant of Pseudomonas aeruginosa azurin
journal, January 2014

  • Sieracki, N. A.; Tian, S.; Hadt, R. G.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 3
  • DOI: 10.1073/pnas.1316483111

Redesigning the Blue Copper Azurin into a Redox-Active Mononuclear Nonheme Iron Protein: Preparation and Study of Fe(II)-M121E Azurin
journal, August 2014

  • Liu, Jing; Meier, Katlyn K.; Tian, Shiliang
  • Journal of the American Chemical Society, Vol. 136, Issue 35
  • DOI: 10.1021/ja505410u

Nitrosocyanin, a Red Cupredoxin-like Protein from Nitrosomonas europaea †
journal, February 2002

  • Arciero, David M.; Pierce, Brad S.; Hendrich, Michael P.
  • Biochemistry, Vol. 41, Issue 6
  • DOI: 10.1021/bi015908w

Complete Genome Sequence of the Ammonia-Oxidizing Bacterium and Obligate Chemolithoautotroph Nitrosomonas europaea
journal, May 2003

Spectroscopic and Density Functional Studies of the Red Copper Site in Nitrosocyanin:  Role of the Protein in Determining Active Site Geometric and Electronic Structure
journal, February 2005

  • Basumallick, Lipika; Sarangi, Ritimukta; DeBeer George, Serena
  • Journal of the American Chemical Society, Vol. 127, Issue 10
  • DOI: 10.1021/ja044412+

Dali server: conservation mapping in 3D
journal, May 2010

  • Holm, Liisa; Rosenstr�m, P�ivi
  • Nucleic Acids Research, Vol. 38, Issue suppl_2
  • DOI: 10.1093/nar/gkq366

Copper coordination in blue proteins
journal, October 2000

  • Gray, Harry B.; Malmström, Bo G.; Williams, R. J. P.
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 5, Issue 5
  • DOI: 10.1007/s007750000146

Crystal Structure of a Novel Red Copper Protein from Nitrosomonas europaea † , ‡
journal, May 2001

  • Lieberman, Raquel L.; Arciero, David M.; Hooper, Alan B.
  • Biochemistry, Vol. 40, Issue 19
  • DOI: 10.1021/bi0102611

Rack-induced metal binding vs. flexibility: Met121His azurin crystal structures at different pH
journal, March 1998

  • Messerschmidt, A.; Prade, L.; Kroes, S. J.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 7
  • DOI: 10.1073/pnas.95.7.3443

Reduction potentials and their pH dependence in site-directed-mutant forms of azurin from Pseudomonas aeruginosa
journal, March 1993

Rationally tuning the reduction potential of a single cupredoxin beyond the natural range
journal, November 2009

  • Marshall, Nicholas M.; Garner, Dewain K.; Wilson, Tiffany D.
  • Nature, Vol. 462, Issue 7269
  • DOI: 10.1038/nature08551

Design of a single protein that spans the entire 2-V range of physiological redox potentials
journal, December 2015

  • Hosseinzadeh, Parisa; Marshall, Nicholas M.; Chacón, Kelly N.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 2
  • DOI: 10.1073/pnas.1515897112

The Role of Hydrogen Bonding at the Active Site of a Cupredoxin:  The Phe114Pro Azurin Variant † , ‡
journal, July 2006

  • Yanagisawa, Sachiko; Banfield, Mark J.; Dennison, Christopher
  • Biochemistry, Vol. 45, Issue 29
  • DOI: 10.1021/bi0606851

Electron Transfer: Cupredoxins
book, January 2003

Spectroscopic Methods in Bioinorganic Chemistry:  Blue to Green to Red Copper Sites
journal, October 2006

Nitric Oxide and Cytochrome c Oxidase: Mechanisms of Inhibition and NO Degradation
journal, July 2000

  • Sarti, Paolo; Giuffré, Alessandro; Forte, Elena
  • Biochemical and Biophysical Research Communications, Vol. 274, Issue 1
  • DOI: 10.1006/bbrc.2000.3117

Effect of Superoxide Dismutase on the Stability ofS-Nitrosothiols
journal, January 1999

  • Jourd'heuil, David; Laroux, F. Stephen; Miles, Allen M.
  • Archives of Biochemistry and Biophysics, Vol. 361, Issue 2
  • DOI: 10.1006/abbi.1998.1010

Copper(I) Nitrosyls from Reaction of Copper(II) Thiolates with S -Nitrosothiols: Mechanism of NO Release from RSNOs at Cu
journal, October 2013

  • Zhang, Shiyu; Çelebi-Ölçüm, Nihan; Melzer, Marie M.
  • Journal of the American Chemical Society, Vol. 135, Issue 45
  • DOI: 10.1021/ja406476y

Spontaneous Catalytic Generation of Nitric Oxide from S -Nitrosothiols at the Surface of Polymer Films Doped with Lipophilic Copper(II) Complex
journal, August 2003

  • Oh, Bong Kyun; Meyerhoff, Mark E.
  • Journal of the American Chemical Society, Vol. 125, Issue 32
  • DOI: 10.1021/ja035775x

Theoretical Evidence that Cu I Complexation Promotes Degradation of S -Nitrosothiols
journal, December 2002

  • Toubin, Céline; Yeung, David Y. -H.; English, Ann M.
  • Journal of the American Chemical Society, Vol. 124, Issue 50
  • DOI: 10.1021/ja027386t

Influence of Cu + on the RS−NO Bond Dissociation Energy of S-Nitrosothiols
journal, February 2005

  • Baciu, Cristina; Cho, Kyung-Bin; Gauld, James W.
  • The Journal of Physical Chemistry B, Vol. 109, Issue 4
  • DOI: 10.1021/jp0443759

Nitric Oxide Donors:  Chemical Activities and Biological Applications
journal, April 2002

  • Wang, Peng George; Xian, Ming; Tang, Xiaoping
  • Chemical Reviews, Vol. 102, Issue 4
  • DOI: 10.1021/cr000040l

Nitric Oxide and Cytochrome c Oxidase: Mechanisms of Inhibition and NO Degradation
journal, July 2000

  • Sarti, Paolo; Giuffré, Alessandro; Forte, Elena
  • Biochemical and Biophysical Research Communications, Vol. 274, Issue 1
  • DOI: 10.1006/bbrc.2000.3117

Enzymatic mechanisms regulating protein S-nitrosylation: implications in health and disease
journal, February 2012

The reaction of nitric oxide with ceruloplasmin
journal, April 1973

  • Wever, R.; Van Leeuwen, F. X. R.; Van Gelder, B. F.
  • Biochimica et Biophysica Acta (BBA) - Enzymology, Vol. 302, Issue 2
  • DOI: 10.1016/0005-2744(73)90152-6

S-Nitrosylation of β-Arrestin Regulates β-Adrenergic Receptor Trafficking
journal, August 2008

What is the real physiological NO concentration in vivo?
journal, September 2009

Crystal Structure of a Novel Red Copper Protein from Nitrosomonas europaea † , ‡
journal, May 2001

  • Lieberman, Raquel L.; Arciero, David M.; Hooper, Alan B.
  • Biochemistry, Vol. 40, Issue 19
  • DOI: 10.1021/bi0102611

Nitrosocyanin, a Red Cupredoxin-like Protein from Nitrosomonas europaea †
journal, February 2002

  • Arciero, David M.; Pierce, Brad S.; Hendrich, Michael P.
  • Biochemistry, Vol. 41, Issue 6
  • DOI: 10.1021/bi015908w

Crystallographic Analysis of the Interaction of Nitric Oxide with Quaternary-T Human Hemoglobin † , ‡
journal, January 2004

  • Chan, Nei-Li; Kavanaugh, Jeffrey S.; Rogers, Paul H.
  • Biochemistry, Vol. 43, Issue 1
  • DOI: 10.1021/bi030172j

The Role of Hydrogen Bonding at the Active Site of a Cupredoxin:  The Phe114Pro Azurin Variant † , ‡
journal, July 2006

  • Yanagisawa, Sachiko; Banfield, Mark J.; Dennison, Christopher
  • Biochemistry, Vol. 45, Issue 29
  • DOI: 10.1021/bi0606851

Nitric Oxide Donors:  Chemical Activities and Biological Applications
journal, April 2002

  • Wang, Peng George; Xian, Ming; Tang, Xiaoping
  • Chemical Reviews, Vol. 102, Issue 4
  • DOI: 10.1021/cr000040l

Spectroscopic Methods in Bioinorganic Chemistry:  Blue to Green to Red Copper Sites
journal, October 2006

Theoretical Evidence that Cu I Complexation Promotes Degradation of S -Nitrosothiols
journal, December 2002

  • Toubin, Céline; Yeung, David Y. -H.; English, Ann M.
  • Journal of the American Chemical Society, Vol. 124, Issue 50
  • DOI: 10.1021/ja027386t

Superoxide Dismutase Targets NO from GSNO to Cysβ93 of Oxyhemoglobin in Concentrated but Not Dilute Solutions of the Protein
journal, November 2003

  • Romeo, Andrea A.; Capobianco, John A.; English, Ann M.
  • Journal of the American Chemical Society, Vol. 125, Issue 47
  • DOI: 10.1021/ja0289752

Spectroscopic and Density Functional Studies of the Red Copper Site in Nitrosocyanin:  Role of the Protein in Determining Active Site Geometric and Electronic Structure
journal, February 2005

  • Basumallick, Lipika; Sarangi, Ritimukta; DeBeer George, Serena
  • Journal of the American Chemical Society, Vol. 127, Issue 10
  • DOI: 10.1021/ja044412+

Outer-Sphere Contributions to the Electronic Structure of Type Zero Copper Proteins
journal, April 2012

  • Lancaster, Kyle M.; Zaballa, María-Eugenia; Sproules, Stephen
  • Journal of the American Chemical Society, Vol. 134, Issue 19
  • DOI: 10.1021/ja302190r

Copper(I) Nitrosyls from Reaction of Copper(II) Thiolates with S -Nitrosothiols: Mechanism of NO Release from RSNOs at Cu
journal, October 2013

  • Zhang, Shiyu; Çelebi-Ölçüm, Nihan; Melzer, Marie M.
  • Journal of the American Chemical Society, Vol. 135, Issue 45
  • DOI: 10.1021/ja406476y

Redesigning the Blue Copper Azurin into a Redox-Active Mononuclear Nonheme Iron Protein: Preparation and Study of Fe(II)-M121E Azurin
journal, August 2014

  • Liu, Jing; Meier, Katlyn K.; Tian, Shiliang
  • Journal of the American Chemical Society, Vol. 136, Issue 35
  • DOI: 10.1021/ja505410u

Heme-assisted S-nitrosation of a proximal thiolate in a nitric oxide transport protein
journal, January 2005

  • Weichsel, A.; Maes, E. M.; Andersen, J. F.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 3
  • DOI: 10.1073/pnas.0406549102

Copper-sulfenate complex from oxidation of a cavity mutant of Pseudomonas aeruginosa azurin
journal, January 2014

  • Sieracki, N. A.; Tian, S.; Hadt, R. G.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 3
  • DOI: 10.1073/pnas.1316483111

A single protein redox ruler
journal, December 2015

  • Bains, Rajneesh K.; Warren, Jeffrey J.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 2
  • DOI: 10.1073/pnas.1522425112

Rack-induced metal binding vs. flexibility: Met121His azurin crystal structures at different pH
journal, March 1998

  • Messerschmidt, A.; Prade, L.; Kroes, S. J.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 7
  • DOI: 10.1073/pnas.95.7.3443

Physiological reactions of nitric oxide and hemoglobin: A radical rethink
journal, August 1999

  • Gross, Steven S.; Lane, Paul
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 18
  • DOI: 10.1073/pnas.96.18.9967

Involvement of Glycosylphosphatidylinositol-linked Ceruloplasmin in the Copper/Zinc-Nitric Oxide-dependent Degradation of Glypican-1 Heparan Sulfate in Rat C6 Glioma Cells
journal, January 2004

  • Mani, Katrin; Cheng, Fang; Havsmark, Birgitta
  • Journal of Biological Chemistry, Vol. 279, Issue 13
  • DOI: 10.1074/jbc.m313678200

S -Nitrosylation-induced Conformational Change in Blackfin Tuna Myoglobin
journal, May 2007

  • Schreiter, Eric R.; Rodríguez, María M.; Weichsel, Andrzej
  • Journal of Biological Chemistry, Vol. 282, Issue 27
  • DOI: 10.1074/jbc.m701363200

Dali server: conservation mapping in 3D
journal, May 2010

  • Holm, Liisa; Rosenstr�m, P�ivi
  • Nucleic Acids Research, Vol. 38, Issue suppl_2
  • DOI: 10.1093/nar/gkq366

Reduction potentials and their pH dependence in site-directed-mutant forms of azurin from Pseudomonas aeruginosa
journal, March 1993

Activation of the Cardiac Calcium Release Channel (Ryanodine Receptor) by Poly-S-Nitrosylation
journal, January 1998

Lessons from the Genome of a Lithoautotroph: Making Biomass from Almost Nothing
journal, May 2003

Physiologic and Proteomic Evidence for a Role of Nitric Oxide in Biofilm Formation by Nitrosomonas europaea and Other Ammonia Oxidizers
journal, April 2004

CHEMICAL PHYSIOLOGY OF BLOOD FLOW REGULATION BY RED BLOOD CELLS:: The Role of Nitric Oxide and S-Nitrosohemoglobin
journal, March 2005

    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    Cupredoxin engineered upconversion nanoparticles for ratiometric luminescence sensing of Cu 2+
    journal, January 2019

    • Liu, Chang; Yu, Yingjie; Chen, Daquan
    • Nanoscale Advances, Vol. 1, Issue 7
    • DOI: 10.1039/c9na00168a

    A Water-Bridged Cysteine-Cysteine Redox Regulation Mechanism in Bacterial Protein Tyrosine Phosphatases
    journal, October 2017

      Sort by:
      [ × clear filter / sort ]

      Similar Records in DOE PAGES and OSTI.GOV collections: