Stacking open-capillary electroosmotic pumps in series to boost the pumping pressure to drive high-performance liquid chromatographic separations
Abstract
Numerous micropumps have been developed, but few of them can produce adequate flow rate and pressure for high-performance liquid chromatography (HPLC) applications. We have recently developed an innovative hybrid electroosmotic pump (EOP) to solve this problem. The basic unit of a hybrid pump consists of a +EOP (the pumping element is positively charged) and a -EOP (the pumping element is negatively charged). The outlet of the +EOP is then joined with the inlet of the -EOP, forming a basic pump unit, while the anode of a positive high voltage (HV) power supply is placed at the joint. The inlet and outlet of this pump unit are electrically grounded. With this configuration, we can stack many of such pump units in series to boost the pumping power. In this work, we describe in details how an open-capillary hybrid EOP is constructed and characterize this pump systematically. We also show that a hybrid EOP with ten serially stacked pump units can deliver a maximum pressure of 21.5 MPa (~3100 psi). Here, we further demonstrate the feasibility of using this hybrid EOP to drive eluents for HPLC separations of proteins and peptides.
- Authors:
-
- Wuhan Textile Univ., Wuhan (China)
- Univ. of Oklahoma, Norman, OK (United States)
- Publication Date:
- Research Org.:
- MicroChem Solutions, Norman, OK (United States)
- Sponsoring Org.:
- USDOE
- Contributing Org.:
- University of Oklahoma
- OSTI Identifier:
- 1337174
- Grant/Contract Number:
- SC0006351
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Chromatography
- Additional Journal Information:
- Journal Volume: 1227; Journal Issue: C; Journal ID: ISSN 0021-9673
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 42 ENGINEERING; serially stacked electroosmotic pump; high performance liquid chromatography; separation
Citation Formats
He, Chiyang, Zhu, Zaifang, Gu, Congying, Lu, Joann, and Liu, Shaorong. Stacking open-capillary electroosmotic pumps in series to boost the pumping pressure to drive high-performance liquid chromatographic separations. United States: N. p., 2012.
Web. doi:10.1016/j.chroma.2011.12.105.
He, Chiyang, Zhu, Zaifang, Gu, Congying, Lu, Joann, & Liu, Shaorong. Stacking open-capillary electroosmotic pumps in series to boost the pumping pressure to drive high-performance liquid chromatographic separations. United States. https://doi.org/10.1016/j.chroma.2011.12.105
He, Chiyang, Zhu, Zaifang, Gu, Congying, Lu, Joann, and Liu, Shaorong. Mon .
"Stacking open-capillary electroosmotic pumps in series to boost the pumping pressure to drive high-performance liquid chromatographic separations". United States. https://doi.org/10.1016/j.chroma.2011.12.105. https://www.osti.gov/servlets/purl/1337174.
@article{osti_1337174,
title = {Stacking open-capillary electroosmotic pumps in series to boost the pumping pressure to drive high-performance liquid chromatographic separations},
author = {He, Chiyang and Zhu, Zaifang and Gu, Congying and Lu, Joann and Liu, Shaorong},
abstractNote = {Numerous micropumps have been developed, but few of them can produce adequate flow rate and pressure for high-performance liquid chromatography (HPLC) applications. We have recently developed an innovative hybrid electroosmotic pump (EOP) to solve this problem. The basic unit of a hybrid pump consists of a +EOP (the pumping element is positively charged) and a -EOP (the pumping element is negatively charged). The outlet of the +EOP is then joined with the inlet of the -EOP, forming a basic pump unit, while the anode of a positive high voltage (HV) power supply is placed at the joint. The inlet and outlet of this pump unit are electrically grounded. With this configuration, we can stack many of such pump units in series to boost the pumping power. In this work, we describe in details how an open-capillary hybrid EOP is constructed and characterize this pump systematically. We also show that a hybrid EOP with ten serially stacked pump units can deliver a maximum pressure of 21.5 MPa (~3100 psi). Here, we further demonstrate the feasibility of using this hybrid EOP to drive eluents for HPLC separations of proteins and peptides.},
doi = {10.1016/j.chroma.2011.12.105},
journal = {Journal of Chromatography},
number = C,
volume = 1227,
place = {United States},
year = {Mon Jan 09 00:00:00 EST 2012},
month = {Mon Jan 09 00:00:00 EST 2012}
}
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Works referencing / citing this record:
High-Pressure Open-Channel On-Chip Electroosmotic Pump for Nanoflow High Performance Liquid Chromatography
journal, February 2014
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