MBTH: A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates

Van Wychen, Stefanie; Long, William; Black, Stuart K.; Laurens, Lieve M. L.

doi:10.1016/j.ab.2016.11.014

Title: MBTH: A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates

Full Record
Other Related Research

Abstract

A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. As a result, the MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 ug mL^-1 without interference from other algae acidic hydrolyzate components.

Authors:

Van Wychen, Stefanie ^[1]; Long, William ^[1]; Black, Stuart K. ^[1];

^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)

Publication Date:: Thu Nov 24 00:00:00 EST 2016

Research Org.:: National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

OSTI Identifier:: 1335584

Alternate Identifier(s):: OSTI ID: 1396852

Report Number(s):: NREL/JA-5100-67563
Journal ID: ISSN 0003-2697

Grant/Contract Number:: AC36-08GO28308; AC36-08-GO28308

Resource Type:: Accepted Manuscript

Journal Name:: Analytical Biochemistry

Additional Journal Information:: Journal Volume: 518; Journal Issue: C; Journal ID: ISSN 0003-2697

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; algae; carbohydrates; spectrophotometric; phenol-sulfuric acid; MBTH

Citation Formats


                    Van Wychen, Stefanie, Long, William, Black, Stuart K., and Laurens, Lieve M. L. MBTH: A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates.  United States: N. p., 2016. 
Web.  doi:10.1016/j.ab.2016.11.014.

Copy to clipboard


                    Van Wychen, Stefanie, Long, William, Black, Stuart K., & Laurens, Lieve M. L. MBTH: A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates.  United States.  https://doi.org/10.1016/j.ab.2016.11.014

Copy to clipboard


                    Van Wychen, Stefanie, Long, William, Black, Stuart K., and Laurens, Lieve M. L. Thu .  
"MBTH: A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates".  United States.  https://doi.org/10.1016/j.ab.2016.11.014.  https://www.osti.gov/servlets/purl/1335584.

Copy to clipboard


                    
@article{osti_1335584,

  title        = {MBTH: A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates},

  author       = {Van Wychen, Stefanie and Long, William and Black, Stuart K. and Laurens, Lieve M. L.},

  abstractNote = {A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. As a result, the MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 ug mL-1 without interference from other algae acidic hydrolyzate components.},

  doi          = {10.1016/j.ab.2016.11.014},

  journal      = {Analytical Biochemistry},

  number       = C,

  volume       = 518,

  place        = {United States},

  year         = {Thu Nov 24 00:00:00 EST 2016},

  month        = {Thu Nov 24 00:00:00 EST 2016}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (Publisher)

Accepted Manuscript (DOE)

Publisher's Version of Record

https://doi.org/10.1016/j.ab.2016.11.014

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 14 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE PAGES and OSTI.GOV collections:

Quantifying the intrinsic value of algal biomass based on a multi-product biorefining strategy

Journal Article Klein, Bruno C. ; Davis, Ryan E. ; Laurens, Lieve M. L. - Algal Research

Microalgal biomass is poised to become an important feedstock in the quest to decarbonize the fuels and chemical industries. In this way, it is imperative that researchers prioritize efforts for increased chances of economic viability of the microalgae biomass supply chain. This work applies a sequential techno-economic assessment (TEA) methodology to compare the economic trade-offs between microalgae growth and compositional shift while solving for an alternative economic metric: intrinsic algal biomass value, i.e., the revenue obtained from processing and converting the main components of algae biomass - carbohydrates, lipids, and protein - to a portfolio of products. The framework reliesmore »« less
https://doi.org/10.1016/j.algal.2023.103094
Determination of Total Carbohydrates in Algal Biomass: Laboratory Analytical Procedure (LAP)

Technical Report Van Wychen, Stefanie ; Laurens, Lieve M. L.

This procedure uses two-step sulfuric acid hydrolysis to hydrolyze the polymeric forms of carbohydrates in algal biomass into monomeric subunits. The monomers are then quantified by either HPLC or a suitable spectrophotometric method.
https://doi.org/10.2172/1118073

Full Text Available
Determination of Total Carbohydrates in Algal Biomass: Laboratory Analytical Procedure (LAP)

Technical Report Van Wychen, Stefanie ; Laurens, Lieve M. L.

This Laboratory Analytical Procedure (LAP) uses a two-step sulfuric acid hydrolysis to hydrolyze polymeric forms of carbohydrates in algal biomass into monomeric subunits. The monomers are then quantified by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) or a suitable spectrophotometric method.
https://doi.org/10.2172/2208753

Full Text Available
Combined algal processing: A novel integrated biorefinery process to produce algal biofuels and bioproducts

Journal Article Dong, Tao ; Knoshaug, Eric P. ; Davis, Ryan ; ... - Algal Research

Here, the development of an integrated biorefinery process capable of producing multiple products is crucial for commercialization of microalgal biofuel production. Dilute acid pretreatment has been demonstrated as an efficient approach to utilize algal biomass more fully, by hydrolyzing microalgal carbohydrates into fermentable sugars, while making the lipids more extractable, and a protein fraction available for other products. Previously, we have shown that sugar-rich liquor could be separated from solid residue by solid-liquid separation (SLS) to produce ethanol via fermentation. However, process modeling has revealed that approximately 37% of the soluble sugars were lost in the solid cake after themore »« less
Cited by 125
https://doi.org/10.1016/j.algal.2015.12.021
Accurate Quantification of Laminarin in Marine Organic Matter with Enzymes from Marine Microbes

Journal Article Becker, Stefan ; Scheffel, André ; Polz, Martin F. ; ... - Applied and Environmental Microbiology

Marine algae produce a variety of glycans, which fulfill diverse biological functions and fuel the carbon and energy demands of heterotrophic microbes. A common approach to analysis of marine organic matter uses acid to hydrolyze the glycans into measurable monosaccharides. The monosaccharides may be derived from different glycans that are built with the same monosaccharides, however, and this approach does not distinguish between glycans in natural samples. Here we use enzymes to digest selectively and thereby quantify laminarin in particulate organic matter. Environmental metaproteome data revealed carbohydrate-active enzymes from marine flavobacteria as tools for selective hydrolysis of the algal β-glucanmore »« less
Cited by 47
https://doi.org/10.1128/aem.03389-16

Full Text Available

Similar Records