Downregulation of a UDP-Arabinomutase Gene in Switchgrass (Panicum virgatum L.) Results in Increased Cell Wall Lignin While Reducing Arabinose-Glycans
Abstract
Switchgrass (Panicum virgatum L.) is a C4 perennial prairie grass and a dedicated feedstock for lignocellulosic biofuels. Saccharification and biofuel yields are inhibited by the plant cell wall's natural recalcitrance against enzymatic degradation. Plant hemicellulose polysaccharides such as arabinoxylans structurally support and cross-link other cell wall polymers. Grasses predominately have Type II cell walls that are abundant in arabinoxylan, which comprise nearly 25% of aboveground biomass. A primary component of arabinoxylan synthesis is uridine diphosphate (UDP) linked to arabinofuranose (Araf). A family of UDP-arabinopyranose mutase (UAM)/reversible glycosylated polypeptides catalyze the interconversion between UDP-arabinopyranose (UDP-Arap) and UDP-Araf. The expression of a switchgrass arabinoxylan biosynthesis pathway gene, PvUAM1, was decreased via RNAi to investigate its role in cell wall recalcitrance in the feedstock. PvUAM1 encodes a switchgrass homolog of UDP-arabinose mutase, which converts UDP-Arap to UDP-Araf. Southern blot analysis revealed each transgenic line contained between one to at least seven T-DNA insertions, resulting in some cases, a 95% reduction of native PvUAM1 transcript in stem internodes. Transgenic plants had increased pigmentation in vascular tissues at nodes, but were otherwise similar in morphology to the non-transgenic control. Cell wall-associated arabinose was decreased in leaves and stems by over 50%, but there was anmore »
- Authors:
-
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Plant Sciences; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Samuel Roberts Noble Foundation, Ardmore, OK (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Univ. of Georgia, Athens, GA (United States). Plant Biology
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1334742
- Report Number(s):
- NREL/JA-2700-67522
Journal ID: ISSN 1664-462X
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; switchgrass; hemicellulosearabinoxylan; UDP-arabinopyranosemutase/reversibleglycosylated polypeptide; biofuel; recalcitrance
Citation Formats
Willis, Jonathan D., Smith, James A., Mazarei, Mitra, Zhang, Ji-Yi, Turner, Geoffrey B., Decker, Stephen R., Sykes, Robert W., Poovaiah, Charleson R., Baxter, Holly L., Mann, David G. J., Davis, Mark F., Udvardi, Michael K., Peña, Maria J., Backe, Jason, Bar-Peled, Maor, and Stewart, C. N. Downregulation of a UDP-Arabinomutase Gene in Switchgrass (Panicum virgatum L.) Results in Increased Cell Wall Lignin While Reducing Arabinose-Glycans. United States: N. p., 2016.
Web. doi:10.3389/fpls.2016.01580.
Willis, Jonathan D., Smith, James A., Mazarei, Mitra, Zhang, Ji-Yi, Turner, Geoffrey B., Decker, Stephen R., Sykes, Robert W., Poovaiah, Charleson R., Baxter, Holly L., Mann, David G. J., Davis, Mark F., Udvardi, Michael K., Peña, Maria J., Backe, Jason, Bar-Peled, Maor, & Stewart, C. N. Downregulation of a UDP-Arabinomutase Gene in Switchgrass (Panicum virgatum L.) Results in Increased Cell Wall Lignin While Reducing Arabinose-Glycans. United States. https://doi.org/10.3389/fpls.2016.01580
Willis, Jonathan D., Smith, James A., Mazarei, Mitra, Zhang, Ji-Yi, Turner, Geoffrey B., Decker, Stephen R., Sykes, Robert W., Poovaiah, Charleson R., Baxter, Holly L., Mann, David G. J., Davis, Mark F., Udvardi, Michael K., Peña, Maria J., Backe, Jason, Bar-Peled, Maor, and Stewart, C. N. Wed .
"Downregulation of a UDP-Arabinomutase Gene in Switchgrass (Panicum virgatum L.) Results in Increased Cell Wall Lignin While Reducing Arabinose-Glycans". United States. https://doi.org/10.3389/fpls.2016.01580. https://www.osti.gov/servlets/purl/1334742.
@article{osti_1334742,
title = {Downregulation of a UDP-Arabinomutase Gene in Switchgrass (Panicum virgatum L.) Results in Increased Cell Wall Lignin While Reducing Arabinose-Glycans},
author = {Willis, Jonathan D. and Smith, James A. and Mazarei, Mitra and Zhang, Ji-Yi and Turner, Geoffrey B. and Decker, Stephen R. and Sykes, Robert W. and Poovaiah, Charleson R. and Baxter, Holly L. and Mann, David G. J. and Davis, Mark F. and Udvardi, Michael K. and Peña, Maria J. and Backe, Jason and Bar-Peled, Maor and Stewart, C. N.},
abstractNote = {Switchgrass (Panicum virgatum L.) is a C4 perennial prairie grass and a dedicated feedstock for lignocellulosic biofuels. Saccharification and biofuel yields are inhibited by the plant cell wall's natural recalcitrance against enzymatic degradation. Plant hemicellulose polysaccharides such as arabinoxylans structurally support and cross-link other cell wall polymers. Grasses predominately have Type II cell walls that are abundant in arabinoxylan, which comprise nearly 25% of aboveground biomass. A primary component of arabinoxylan synthesis is uridine diphosphate (UDP) linked to arabinofuranose (Araf). A family of UDP-arabinopyranose mutase (UAM)/reversible glycosylated polypeptides catalyze the interconversion between UDP-arabinopyranose (UDP-Arap) and UDP-Araf. The expression of a switchgrass arabinoxylan biosynthesis pathway gene, PvUAM1, was decreased via RNAi to investigate its role in cell wall recalcitrance in the feedstock. PvUAM1 encodes a switchgrass homolog of UDP-arabinose mutase, which converts UDP-Arap to UDP-Araf. Southern blot analysis revealed each transgenic line contained between one to at least seven T-DNA insertions, resulting in some cases, a 95% reduction of native PvUAM1 transcript in stem internodes. Transgenic plants had increased pigmentation in vascular tissues at nodes, but were otherwise similar in morphology to the non-transgenic control. Cell wall-associated arabinose was decreased in leaves and stems by over 50%, but there was an increase in cellulose. In addition, there was a commensurate change in arabinose side chain extension. Cell wall lignin composition was altered with a concurrent increase in lignin content and transcript abundance of lignin biosynthetic genes in mature tillers. Enzymatic saccharification efficiency was unchanged in the transgenic plants relative to the control. Plants with attenuated PvUAM1 transcript had increased cellulose and lignin in cell walls. A decrease in cell wall-associated arabinose was expected, which was likely caused by fewer Araf residues in the arabinoxylan. The decrease in arabinoxylan may cause a compensation response to maintain cell wall integrity by increasing cellulose and lignin biosynthesis. In cases in which increased lignin is desired, e.g., feedstocks for carbon fiber production, downregulated UAM1 coupled with altered expression of other arabinoxylan biosynthesis genes might result in even higher production of lignin in biomass.},
doi = {10.3389/fpls.2016.01580},
journal = {Frontiers in Plant Science},
number = ,
volume = 7,
place = {United States},
year = {Wed Oct 26 00:00:00 EDT 2016},
month = {Wed Oct 26 00:00:00 EDT 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 17 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Genetic and biotechnological approaches for biofuel crop improvement
journal, April 2010
- Vega-Sánchez, Miguel E.; Ronald, Pamela C.
- Current Opinion in Biotechnology, Vol. 21, Issue 2
A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers
journal, September 2016
- Harman-Ware, Anne E.; Foster, Cliff; Happs, Renee M.
- Biotechnology Journal, Vol. 11, Issue 10
Ether linkage between phenolic acids and lignin fractions from wheat straw
journal, January 1985
- Scalbert, A.
- Phytochemistry, Vol. 24, Issue 6
Xylan biosynthesis
journal, April 2014
- Rennie, Emilie A.; Scheller, Henrik Vibe
- Current Opinion in Biotechnology, Vol. 26
Phylogeny.fr: robust phylogenetic analysis for the non-specialist
journal, May 2008
- Dereeper, A.; Guignon, V.; Blanc, G.
- Nucleic Acids Research, Vol. 36, Issue Web Server
The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose Is Indispensable for Plant Development in Arabidopsis
journal, April 2011
- Rautengarten, Carsten; Ebert, Berit; Herter, Thomas
- The Plant Cell, Vol. 23, Issue 4
Analyzing real-time PCR data by the comparative CT method
journal, June 2008
- Schmittgen, Thomas D.; Livak, Kenneth J.
- Nature Protocols, Vol. 3, Issue 6
Grass cell wall feruloylation: distribution of bound ferulate and candidate gene expression in Brachypodium distachyon
journal, January 2013
- Molinari, Hugo B. C.; Pellny, Till K.; Freeman, Jackie
- Frontiers in Plant Science, Vol. 4
Approximate Likelihood-Ratio Test for Branches: A Fast, Accurate, and Powerful Alternative
journal, August 2006
- Anisimova, Maria; Gascuel, Olivier
- Systematic Biology, Vol. 55, Issue 4
Xylan Biosynthesis: News from the Grass
journal, April 2010
- Faik, Ahmed
- Plant Physiology, Vol. 153, Issue 2
ORIGINAL RESEARCH: Lignocellulose recalcitrance screening by integrated high-throughput hydrothermal pretreatment and enzymatic saccharification
journal, April 2010
- Selig, Michael J.; Tucker, Melvin P.; Sykes, Robert W.
- Industrial Biotechnology, Vol. 6, Issue 2
Biochemical control of xylan biosynthesis — which end is up?
journal, June 2008
- York, W.; Oneill, M.
- Current Opinion in Plant Biology, Vol. 11, Issue 3
An Improved Tissue Culture System for Embryogenic Callus Production and Plant Regeneration in Switchgrass (Panicum virgatum L.)
journal, October 2009
- Burris, Jason N.; Mann, David G. J.; Joyce, Blake L.
- BioEnergy Research, Vol. 2, Issue 4
Incorporation of Proline and Aromatic Amino Acids into Cell Walls of Maize Coleoptiles
journal, March 1986
- Carpita, Nicholas C.
- Plant Physiology, Vol. 80, Issue 3
Structure and Biogenesis of the cell Walls of Grasses
journal, June 1996
- Carpita, Nicholas C.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 47, Issue 1
Expression of cell wall related genes in basal and ear internodes of silking brown-midrib-3, caffeic acid O-methyltransferase (COMT) down-regulated, and normal maize plants
journal, January 2008
- Guillaumie, Sabine; Goffner, Deborah; Barbier, Odile
- BMC Plant Biology, Vol. 8, Issue 1
Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses
journal, January 2012
- Anders, N.; Wilkinson, M. D.; Lovegrove, A.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 3
Two-year field analysis of reduced recalcitrance transgenic switchgrass
journal, April 2014
- Baxter, Holly L.; Mazarei, Mitra; Labbe, Nicole
- Plant Biotechnology Journal, Vol. 12, Issue 7
Overexpression of miR156 in switchgrass (Panicum virgatum L.) results in various morphological alterations and leads to improved biomass production: Overexpression of miR156 improved switchgrass biomass
journal, January 2012
- Fu, Chunxiang; Sunkar, Ramanjulu; Zhou, Chuanen
- Plant Biotechnology Journal, Vol. 10, Issue 4
Arabinoxylan Biosynthesis in Wheat. Characterization of Arabinosyltransferase Activity in Golgi Membranes
journal, August 2002
- Porchia, Andrea Celia; Sørensen, Susanne Oxenbøll; Scheller, Henrik Vibe
- Plant Physiology, Vol. 130, Issue 1
Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks
journal, October 2011
- Shen, Hui; He, Xianzhi; Poovaiah, Charleson R.
- New Phytologist, Vol. 193, Issue 1, p. 121-136
Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011
- Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
Monitoring switchgrass composition to optimize harvesting periods for bioenergy and value-added products
journal, September 2013
- Lindsey, Kline; Johnson, Amy; Kim, Pyoungchung
- Biomass and Bioenergy, Vol. 56
Feruloylation in Grasses: Current and Future Perspectives
journal, September 2009
- de O. Buanafina, Marcia M.
- Molecular Plant, Vol. 2, Issue 5
A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose
journal, December 2006
- Konishi, Teruko; Takeda, Takumi; Miyazaki, Yasumasa
- Glycobiology, Vol. 17, Issue 3
An arginyl residue in rice UDP-arabinopyranose mutase is required for catalytic activity and autoglycosylation
journal, April 2010
- Konishi, Tomoyuki; Ohnishi-Kameyama, Mayumi; Funane, Kazumi
- Carbohydrate Research, Vol. 345, Issue 6
High throughput Agrobacterium-mediated switchgrass transformation
journal, March 2011
- Li, Ruyu; Qu, Rongda
- Biomass and Bioenergy, Vol. 35, Issue 3
Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011
- Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
An Improved Tissue Culture System for Embryogenic Callus Production and Plant Regeneration in Switchgrass (Panicum virgatum L.)
journal, October 2009
- Burris, Jason N.; Mann, David G. J.; Joyce, Blake L.
- BioEnergy Research, Vol. 2, Issue 4
Gateway-compatible vectors for high-throughput gene functional analysis in switchgrass (Panicum virgatum L.) and other monocot species: Gateway-compatible vectors for monocot transformation
journal, September 2011
- Mann, David G. J.; LaFayette, Peter R.; Abercrombie, Laura L.
- Plant Biotechnology Journal, Vol. 10, Issue 2
An Arabidopsis Cell Wall Proteoglycan Consists of Pectin and Arabinoxylan Covalently Linked to an Arabinogalactan Protein
journal, January 2013
- Tan, Li; Eberhard, Stefan; Pattathil, Sivakumar
- The Plant Cell, Vol. 25, Issue 1
Genome-Scale Identification of Cell-Wall-Related Genes in Switchgrass through Comparative Genomics and Computational Analyses of Transcriptomic Data
journal, September 2015
- Chen, Xin; Ma, Qin; Rao, Xiaolan
- BioEnergy Research, Vol. 9, Issue 1
Identifying critical unrecognized sugar-protein interactions in GH10 xylanases from Geobacillus stearothermophilus using STD NMR
journal, August 2013
- Balazs, Yael S.; Lisitsin, Elina; Carmiel, Oshrat
- FEBS Journal, Vol. 280, Issue 18
Biosynthesis of UDP-Xylose. Cloning and Characterization of a Novel Arabidopsis Gene Family, UXS , Encoding Soluble and Putative Membrane-Bound UDP-Glucuronic Acid Decarboxylase Isoforms
journal, December 2002
- Harper, April D.; Bar-Peled, Maor
- Plant Physiology, Vol. 130, Issue 4
ORIGINAL RESEARCH: Lignocellulose recalcitrance screening by integrated high-throughput hydrothermal pretreatment and enzymatic saccharification
journal, April 2010
- Selig, Michael J.; Tucker, Melvin P.; Sykes, Robert W.
- Industrial Biotechnology, Vol. 6, Issue 2
Plant Nucleotide Sugar Formation, Interconversion, and Salvage by Sugar Recycling*
journal, June 2011
- Bar-Peled, Maor; O'Neill, Malcolm A.
- Annual Review of Plant Biology, Vol. 62, Issue 1
Semimicro determination of cellulose inbiological materials
journal, December 1969
- Updegraff, David M.
- Analytical Biochemistry, Vol. 32, Issue 3
Inhibition of acetyl-CoA carboxylase by spirotetramat causes growth arrest and lipid depletion in nematodes
journal, July 2020
- Gutbrod, Philipp; Gutbrod, Katharina; Nauen, Ralf
- Scientific Reports, Vol. 10, Issue 1
Cell wall composition and digestibility alterations in Brachypodium distachyon achieved through reduced expression of the UDP-arabinopyranose mutase
journal, June 2015
- Rancour, David M.; Hatfield, Ronald D.; Marita, Jane M.
- Frontiers in Plant Science, Vol. 6
High-Throughput Screening of Plant Cell-Wall Composition Using Pyrolysis Molecular Beam Mass Spectroscopy
book, January 2009
- Sykes, Robert; Yung, Matthew; Novaes, Evandro
- Biofuels
Xylan Biosynthesis: News from the Grass
journal, April 2010
- Faik, Ahmed
- Plant Physiology, Vol. 153, Issue 2
Two-year field analysis of reduced recalcitrance transgenic switchgrass
journal, April 2014
- Baxter, Holly L.; Mazarei, Mitra; Labbe, Nicole
- Plant Biotechnology Journal, Vol. 12, Issue 7
Hemicelluloses
journal, June 2010
- Scheller, Henrik Vibe; Ulvskov, Peter
- Annual Review of Plant Biology, Vol. 61, Issue 1
Xylan biosynthesis
journal, April 2014
- Rennie, Emilie A.; Scheller, Henrik Vibe
- Current Opinion in Biotechnology, Vol. 26
Down-regulation of UDP-arabinopyranose mutase reduces the proportion of arabinofuranose present in rice cell walls
journal, November 2011
- Konishi, Tomoyuki; Aohara, Tsutomu; Igasaki, Tomohiro
- Phytochemistry, Vol. 72, Issue 16
Very bright orange fluorescent plants: endoplasmic reticulum targeting of orange fluorescent proteins as visual reporters in transgenic plants
journal, January 2012
- Mann, David GJ; Abercrombie, Laura L.; Rudis, Mary R.
- BMC Biotechnology, Vol. 12, Issue 1
Genetic and biotechnological approaches for biofuel crop improvement
journal, April 2010
- Vega-Sánchez, Miguel E.; Ronald, Pamela C.
- Current Opinion in Biotechnology, Vol. 21, Issue 2
Expression of cell wall related genes in basal and ear internodes of silking brown-midrib-3, caffeic acid O-methyltransferase (COMT) down-regulated, and normal maize plants
journal, January 2008
- Guillaumie, Sabine; Goffner, Deborah; Barbier, Odile
- BMC Plant Biology, Vol. 8, Issue 1
Structure and Biogenesis of the cell Walls of Grasses
journal, June 1996
- Carpita, Nicholas C.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 47, Issue 1
Covalent Cross-Links in the Cell Wall
journal, February 1994
- Iiyama, K.; Lam, Tbt.; Stone, B. A.
- Plant Physiology, Vol. 104, Issue 2
Cell wall composition and digestibility alterations in Brachypodium distachyon achieved through reduced expression of the UDP-arabinopyranose mutase
journal, June 2015
- Rancour, David M.; Hatfield, Ronald D.; Marita, Jane M.
- Frontiers in Plant Science, Vol. 6
High-Throughput Screening of Plant Cell-Wall Composition Using Pyrolysis Molecular Beam Mass Spectroscopy
book, January 2009
- Sykes, Robert; Yung, Matthew; Novaes, Evandro
- Biofuels
Grass cell wall feruloylation: distribution of bound ferulate and candidate gene expression in Brachypodium distachyon
journal, January 2013
- Molinari, Hugo B. C.; Pellny, Till K.; Freeman, Jackie
- Frontiers in Plant Science, Vol. 4
XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan
journal, October 2012
- Chiniquy, D.; Sharma, V.; Schultink, A.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 42
Identifying critical unrecognized sugar-protein interactions in GH10 xylanases from Geobacillus stearothermophilus using STD NMR
journal, August 2013
- Balazs, Yael S.; Lisitsin, Elina; Carmiel, Oshrat
- FEBS Journal, Vol. 280, Issue 18
Effect of arabinose substitution on the material properties of arabinoxylan films
journal, March 2008
- Sternemalm, Erik; Höije, Anders; Gatenholm, Paul
- Carbohydrate Research, Vol. 343, Issue 4
The Switchgrass Genome: Tools and Strategies
journal, January 2011
- Casler, Michael D.; Tobias, Christian M.; Kaeppler, Shawn M.
- The Plant Genome Journal, Vol. 4, Issue 3
Silencing of 4-coumarate:coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production
journal, July 2011
- Xu, Bin; Escamilla-Treviño, Luis L.; Sathitsuksanoh, Noppadon
- New Phytologist, Vol. 192, Issue 3
Incorporation of Proline and Aromatic Amino Acids into Cell Walls of Maize Coleoptiles
journal, March 1986
- Carpita, Nicholas C.
- Plant Physiology, Vol. 80, Issue 3
Plant Nucleotide Sugar Formation, Interconversion, and Salvage by Sugar Recycling*
journal, June 2011
- Bar-Peled, Maor; O'Neill, Malcolm A.
- Annual Review of Plant Biology, Vol. 62, Issue 1
MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
journal, October 2013
- Tamura, Koichiro; Stecher, Glen; Peterson, Daniel
- Molecular Biology and Evolution, Vol. 30, Issue 12
Phylogeny.fr: robust phylogenetic analysis for the non-specialist
journal, May 2008
- Dereeper, A.; Guignon, V.; Blanc, G.
- Nucleic Acids Research, Vol. 36, Issue Web Server
The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose Is Indispensable for Plant Development in Arabidopsis
journal, April 2011
- Rautengarten, Carsten; Ebert, Berit; Herter, Thomas
- The Plant Cell, Vol. 23, Issue 4
Developmental Control of Lignification in Stems of Lowland Switchgrass Variety Alamo and the Effects on Saccharification Efficiency
journal, October 2009
- Shen, Hui; Fu, Chunxiang; Xiao, Xirong
- BioEnergy Research, Vol. 2, Issue 4
Field Evaluation of Transgenic Switchgrass Plants Overexpressing PvMYB4 for Reduced Biomass Recalcitrance
journal, January 2015
- Baxter, Holly L.; Poovaiah, Charleson R.; Yee, Kelsey L.
- BioEnergy Research, Vol. 8, Issue 3
Purification and Characterization of UDP-Arabinopyranose Mutase from Chlamydomonas reinhardtii
journal, September 2013
- Kotani, Ayana; Tsuji, Maki; Azama, Yasushi
- Bioscience, Biotechnology, and Biochemistry, Vol. 77, Issue 9
Isolation and characterization of plant cell walls and cell wall components
book, January 1986
- York, William S.; Darvill, Alan G.; McNeil, Michael
- Methods in Enzymology
Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014
- Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
- Science, Vol. 344, Issue 6185, p. 1246843-1246843
Overexpression of miR156 in switchgrass (Panicum virgatum L.) results in various morphological alterations and leads to improved biomass production: Overexpression of miR156 improved switchgrass biomass
journal, January 2012
- Fu, Chunxiang; Sunkar, Ramanjulu; Zhou, Chuanen
- Plant Biotechnology Journal, Vol. 10, Issue 4
Arabinoxylan Biosynthesis in Wheat. Characterization of Arabinosyltransferase Activity in Golgi Membranes
journal, August 2002
- Porchia, Andrea Celia; Sørensen, Susanne Oxenbøll; Scheller, Henrik Vibe
- Plant Physiology, Vol. 130, Issue 1
Cell wall composition throughout development for the model grass Brachypodium distachyon
journal, January 2012
- Rancour, David M.; Marita, Jane M.; Hatfield, Ronald D.
- Frontiers in Plant Science, Vol. 3
Cell wall composition throughout development for the model grass Brachypodium distachyon
journal, January 2012
- Rancour, David M.; Marita, Jane M.; Hatfield, Ronald D.
- Frontiers in Plant Science, Vol. 3
An Arabidopsis Cell Wall Proteoglycan Consists of Pectin and Arabinoxylan Covalently Linked to an Arabinogalactan Protein
journal, January 2013
- Tan, Li; Eberhard, Stefan; Pattathil, Sivakumar
- The Plant Cell, Vol. 25, Issue 1
A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose
journal, December 2006
- Konishi, Teruko; Takeda, Takumi; Miyazaki, Yasumasa
- Glycobiology, Vol. 17, Issue 3
Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses
journal, January 2012
- Anders, N.; Wilkinson, M. D.; Lovegrove, A.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 3
Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks
journal, October 2011
- Shen, Hui; He, Xianzhi; Poovaiah, Charleson R.
- New Phytologist, Vol. 193, Issue 1, p. 121-136
Developmental Control of Lignification in Stems of Lowland Switchgrass Variety Alamo and the Effects on Saccharification Efficiency
journal, October 2009
- Shen, Hui; Fu, Chunxiang; Xiao, Xirong
- BioEnergy Research, Vol. 2, Issue 4
Standardization of Switchgrass Sample Collection for Cell Wall and Biomass Trait Analysis
journal, January 2013
- Hardin, C. Frank; Fu, Chunxiang; Hisano, Hiroshi
- BioEnergy Research, Vol. 6, Issue 2
Genome-Scale Identification of Cell-Wall-Related Genes in Switchgrass through Comparative Genomics and Computational Analyses of Transcriptomic Data
journal, September 2015
- Chen, Xin; Ma, Qin; Rao, Xiaolan
- BioEnergy Research, Vol. 9, Issue 1
Biochemical control of xylan biosynthesis — which end is up?
journal, June 2008
- York, W.; Oneill, M.
- Current Opinion in Plant Biology, Vol. 11, Issue 3
Biosynthesis of UDP-Xylose. Cloning and Characterization of a Novel Arabidopsis Gene Family, UXS , Encoding Soluble and Putative Membrane-Bound UDP-Glucuronic Acid Decarboxylase Isoforms
journal, December 2002
- Harper, April D.; Bar-Peled, Maor
- Plant Physiology, Vol. 130, Issue 4
Field Evaluation of Transgenic Switchgrass Plants Overexpressing PvMYB4 for Reduced Biomass Recalcitrance
journal, January 2015
- Baxter, Holly L.; Poovaiah, Charleson R.; Yee, Kelsey L.
- BioEnergy Research, Vol. 8, Issue 3
Monitoring switchgrass composition to optimize harvesting periods for bioenergy and value-added products
journal, September 2013
- Lindsey, Kline; Johnson, Amy; Kim, Pyoungchung
- Biomass and Bioenergy, Vol. 56
Down-regulation of UDP-arabinopyranose mutase reduces the proportion of arabinofuranose present in rice cell walls
journal, November 2011
- Konishi, Tomoyuki; Aohara, Tsutomu; Igasaki, Tomohiro
- Phytochemistry, Vol. 72, Issue 16
Very bright orange fluorescent plants: endoplasmic reticulum targeting of orange fluorescent proteins as visual reporters in transgenic plants
journal, January 2012
- Mann, David GJ; Abercrombie, Laura L.; Rudis, Mary R.
- BMC Biotechnology, Vol. 12, Issue 1
Effect of arabinose substitution on the material properties of arabinoxylan films
journal, March 2008
- Sternemalm, Erik; Höije, Anders; Gatenholm, Paul
- Carbohydrate Research, Vol. 343, Issue 4
MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
journal, October 2013
- Tamura, Koichiro; Stecher, Glen; Peterson, Daniel
- Molecular Biology and Evolution, Vol. 30, Issue 12
XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan
journal, October 2012
- Chiniquy, D.; Sharma, V.; Schultink, A.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 42
Plant polypeptides reversibly glycosylated by UDP-glucose. Possible components of Golgi beta-glucan synthase in pea cells.
journal, November 1991
- Dhugga, K. S.; Ulvskov, P.; Gallagher, S. R.
- Journal of Biological Chemistry, Vol. 266, Issue 32
Works referencing / citing this record:
Genetic engineering of grass cell wall polysaccharides for biorefining
journal, June 2017
- Bhatia, Rakesh; Gallagher, Joe A.; Gomez, Leonardo D.
- Plant Biotechnology Journal, Vol. 15, Issue 9
Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass
journal, March 2020
- Brandon, Andrew G.; Scheller, Henrik V.
- Frontiers in Plant Science, Vol. 11
Lignins: Biosynthesis and Biological Functions in Plants
journal, January 2018
- Liu, Qingquan; Luo, Le; Zheng, Luqing
- International Journal of Molecular Sciences, Vol. 19, Issue 2
Lignins: Biosynthesis and Biological Functions in Plants
journal, January 2018
- Liu, Qingquan; Luo, Le; Zheng, Luqing
- International Journal of Molecular Sciences, Vol. 19, Issue 2
Gene regulatory networks for lignin biosynthesis in switchgrass (Panicum virgatum )
journal, September 2018
- Rao, Xiaolan; Chen, Xin; Shen, Hui
- Plant Biotechnology Journal, Vol. 17, Issue 3
Molecular characteristics of plant UDP-arabinopyranose mutases
journal, May 2019
- Saqib, Anam; Scheller, Henrik Vibe; Fredslund, Folmer
- Glycobiology, Vol. 29, Issue 12
Suberin and hemicellulose in sugarcane cell wall architecture and crop digestibility: A biotechnological perspective
journal, February 2019
- Figueiredo, Raquel; Araújo, Pedro; Llerena, Juan Pablo P.
- Food and Energy Security, Vol. 8, Issue 3