Title: Molybdenum Availability Is Key to Nitrate Removal in Contaminated Groundwater Environments

Abstract

The concentrations of molybdenum (Mo) and 25 other metals were measured in groundwater samples from 80 wells on the Oak Ridge Reservation (ORR) (Oak Ridge, TN), many of which are contaminated with nitrate, as well as uranium and various other metals. Moreover, the concentrations of nitrate and uranium were in the ranges of 0.1 μM to 230 mM and <0.2 nM to 580 μM, respectively. Most metals examined had significantly greater median concentrations in a subset of wells that were highly contaminated with uranium (≥126 nM). They included cadmium, manganese, and cobalt, which were 1,300- to 2,700-fold higher. A notable exception, however, was Mo, which had a lower median concentration in the uranium-contaminated wells. This is significant, because Mo is essential in the dissimilatory nitrate reduction branch of the global nitrogen cycle. It is required at the catalytic site of nitrate reductase, the enzyme that reduces nitrate to nitrite. Furthermore, more than 85% of the groundwater samples contained less than 10 nM Mo, whereas concentrations of 10 to 100 nM Mo were required for efficient growth by nitrate reduction for twoPseudomonasstrains isolated from ORR wells and by a model denitrifier,Pseudomonas stutzeriRCH2. Higher concentrations of Mo tended to inhibit the growthmore » of these strains due to the accumulation of toxic concentrations of nitrite, and this effect was exacerbated at high nitrate concentrations. The relevance of these results to a Mo-based nitrate removal strategy and the potential community-driving role that Mo plays in contaminated environments are discussed.« less

Authors:

Thorgersen, Michael P. ^[1]; Lancaster, W. Andrew ^[1]; Vaccaro, Brian J. ^[1]; Poole, Farris L. ^[1]; Rocha, Andrea M. ^[2]; Mehlhorn, Tonia ^[2]; Pettenato, Angelica ^[3]; Ray, Jayashree ^[3]; Waters, R. Jordan ^[3]; Melnyk, Ryan A. ^[3]; Chakraborty, Romy ^[3]; Hazen, Terry C.  ^[2];

• Search DOE PAGES for author "Hazen, Terry C."
• Search DOE PAGES for ORCID "0000-0002-2536-9993"

Deutschbauer, Adam M. ^[3]; Arkin, Adam P. ^[3]; Adams, Michael W. W. ^[1]; Voordouw, G.

---

+ Show Author Affiliations

1. Univ. of Georgia, Athens, GA (United States). Dept. of Biochemistry and Molecular Biology
2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Publication Date:

Fri May 15 00:00:00 EDT 2015

Research Org.:

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:

USDOE Office of Science (SC)

OSTI Identifier:

1334444

Grant/Contract Number:  

AC05-00OR22725

Resource Type:

Accepted Manuscript

Journal Name:

Applied and Environmental Microbiology

Additional Journal Information:

Journal Volume: 81; Journal Issue: 15; Journal ID: ISSN 0099-2240

Publisher:

American Society for Microbiology

Country of Publication:

United States

Language:

English

Subject:

54 ENVIRONMENTAL SCIENCES