Intracellular Hg(0) Oxidation in Desulfovibrio desulfuricans ND132

Wang, Yuwei; Schaefer, Jeffra K.; Mishra, Bhoopesh; Yee, Nathan

doi:10.1021/acs.est.6b03299

Title: Intracellular Hg(0) Oxidation in Desulfovibrio desulfuricans ND132

Abstract

The disposal of elemental mercury (Hg(0)) wastes in mining and manufacturing areas has caused serious soil and groundwater contamination issues. Under anoxic conditions, certain anaerobic bacteria can oxidize dissolved elemental mercury and convert the oxidized Hg to neurotoxic methylmercury. In this study, we conducted experiments with the Hg-methylating bacterium Desulfovibrio desulfuricans ND132 to elucidate the role of cellular thiols in anaerobic Hg(0) oxidation. The concentrations of cell-surface and intracellular thiols were measured, and specific fractions of D. desulfuricans ND132 were examined for Hg(0) oxidation activity and analyzed with extended X-ray absorption fine structure (EXAFS) spectroscopy. The experimental data indicate that intracellular thiol concentrations are approximately six times higher than those of the cell wall. Cells reacted with a thiol-blocking reagent were severely impaired in Hg(0) oxidation activity. Spheroplasts lacking cell walls rapidly oxidized Hg(0) to Hg(II), while cell wall fragments exhibited low reactivity toward Hg(0). EXAFS analysis of spheroplast samples revealed that multiple different forms of Hg-thiols are produced by the Hg(0) oxidation reaction and that the local coordination environment of the oxidized Hg changes with reaction time. Here, the results of this study indicate that Hg(0) oxidation in D. desulfuricans ND132 is an intracellular process that occurs by reactionmore » with thiol-containing molecules.« less

Authors:

Wang, Yuwei ^[1]; Schaefer, Jeffra K. ^[1]; Mishra, Bhoopesh ^[2]; Yee, Nathan ^[1]

Rutgers Univ., New Brunswick, NJ (United States)
Illinois Inst. of Technology, Chicago, IL (United States)

Publication Date:: Wed Sep 21 00:00:00 EDT 2016

Research Org.:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1331675

Resource Type:: Accepted Manuscript

Journal Name:: Environmental Science and Technology

Additional Journal Information:: Journal Volume: 50; Journal Issue: 20; Journal ID: ISSN 0013-936X

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: ENGLISH

Subject:: 54 ENVIRONMENTAL SCIENCES; thiols; extended X-ray absorption fine structure; cells; mercury; oxidation

Citation Formats


                    Wang, Yuwei, Schaefer, Jeffra K., Mishra, Bhoopesh, and Yee, Nathan. Intracellular Hg(0) Oxidation in Desulfovibrio desulfuricans ND132.  United States: N. p., 2016. 
Web.  doi:10.1021/acs.est.6b03299.

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                    Wang, Yuwei, Schaefer, Jeffra K., Mishra, Bhoopesh, & Yee, Nathan. Intracellular Hg(0) Oxidation in Desulfovibrio desulfuricans ND132.  United States.  https://doi.org/10.1021/acs.est.6b03299

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                    Wang, Yuwei, Schaefer, Jeffra K., Mishra, Bhoopesh, and Yee, Nathan. Wed .  
"Intracellular Hg(0) Oxidation in Desulfovibrio desulfuricans ND132".  United States.  https://doi.org/10.1021/acs.est.6b03299.  https://www.osti.gov/servlets/purl/1331675.

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@article{osti_1331675,

  title        = {Intracellular Hg(0) Oxidation in Desulfovibrio desulfuricans ND132},

  author       = {Wang, Yuwei and Schaefer, Jeffra K. and Mishra, Bhoopesh and Yee, Nathan},

  abstractNote = {The disposal of elemental mercury (Hg(0)) wastes in mining and manufacturing areas has caused serious soil and groundwater contamination issues. Under anoxic conditions, certain anaerobic bacteria can oxidize dissolved elemental mercury and convert the oxidized Hg to neurotoxic methylmercury. In this study, we conducted experiments with the Hg-methylating bacterium Desulfovibrio desulfuricans ND132 to elucidate the role of cellular thiols in anaerobic Hg(0) oxidation. The concentrations of cell-surface and intracellular thiols were measured, and specific fractions of D. desulfuricans ND132 were examined for Hg(0) oxidation activity and analyzed with extended X-ray absorption fine structure (EXAFS) spectroscopy. The experimental data indicate that intracellular thiol concentrations are approximately six times higher than those of the cell wall. Cells reacted with a thiol-blocking reagent were severely impaired in Hg(0) oxidation activity. Spheroplasts lacking cell walls rapidly oxidized Hg(0) to Hg(II), while cell wall fragments exhibited low reactivity toward Hg(0). EXAFS analysis of spheroplast samples revealed that multiple different forms of Hg-thiols are produced by the Hg(0) oxidation reaction and that the local coordination environment of the oxidized Hg changes with reaction time. Here, the results of this study indicate that Hg(0) oxidation in D. desulfuricans ND132 is an intracellular process that occurs by reaction with thiol-containing molecules.},

  doi          = {10.1021/acs.est.6b03299},

  journal      = {Environmental Science and Technology},

  number       = 20,

  volume       = 50,

  place        = {United States},

  year         = {Wed Sep 21 00:00:00 EDT 2016},

  month        = {Wed Sep 21 00:00:00 EDT 2016}

}

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Works referenced in this record:

Mercury Speciation and Mobilization in a Wastewater-Contaminated Groundwater Plume
journal, November 2013

Lamborg, Carl H.; Kent, Doug B.; Swarr, Gretchen J.
Environmental Science & Technology, Vol. 47, Issue 23
DOI: 10.1021/es402441d

Current Perspectives in Contaminant Hydrology and Water Resources Sustainability
book, February 2013

Bradley, Paul
DOI: 10.5772/47884

Mercury (II) reduction and co-precipitation of metallic mercury on hydrous ferric oxide in contaminated groundwater
journal, January 2016

Richard, Jan-Helge; Bischoff, Cornelia; Ahrens, Christian G. M.
Science of The Total Environment, Vol. 539
DOI: 10.1016/j.scitotenv.2015.08.116

X-ray Absorption Spectroscopic Evidence for the Complexation of Hg(II) by Reduced Sulfur in Soil Humic Substances
journal, January 1999

Xia, K.; Skyllberg, U. L.; Bleam, W. F.
Environmental Science & Technology, Vol. 33, Issue 2
DOI: 10.1021/es980433q

Interactions between mercury and dissolved organic matter––a review
journal, April 2004

Ravichandran, Mahalingam
Chemosphere, Vol. 55, Issue 3
DOI: 10.1016/j.chemosphere.2003.11.011

Transport of elemental mercury in the unsaturated zone from a waste disposal site in an arid region
journal, March 2008

Walvoord, Michelle A.; Andraski, Brian J.; Krabbenhoft, David P.
Applied Geochemistry, Vol. 23, Issue 3
DOI: 10.1016/j.apgeochem.2007.12.014

Mercury speciation analyses in HgCl2-contaminated soils and groundwater—Implications for risk assessment and remediation strategies
journal, January 2008

Bollen, A.; Wenke, A.; Biester, H.
Water Research, Vol. 42, Issue 1-2
DOI: 10.1016/j.watres.2007.07.011

The Chemical Cycle and Bioaccumulation of Mercury
journal, November 1998

Morel, François M. M.; Kraepiel, Anne M. L.; Amyot, Marc
Annual Review of Ecology and Systematics, Vol. 29, Issue 1
DOI: 10.1146/annurev.ecolsys.29.1.543

Mechanisms Regulating Mercury Bioavailability for Methylating Microorganisms in the Aquatic Environment: A Critical Review
journal, February 2013

Hsu-Kim, Heileen; Kucharzyk, Katarzyna H.; Zhang, Tong
Environmental Science & Technology, Vol. 47, Issue 6
DOI: 10.1021/es304370g

History of mercury use and environmental contamination at the Oak Ridge Y-12 Plant
journal, January 2011

Brooks, Scott C.; Southworth, George R.
Environmental Pollution, Vol. 159, Issue 1
DOI: 10.1016/j.envpol.2010.09.009

Characterization of soils from an industrial complex contaminated with elemental mercury
journal, August 2013

Miller, Carrie L.; Watson, David B.; Lester, Brian P.
Environmental Research, Vol. 125
DOI: 10.1016/j.envres.2013.03.013

Mercury mobility at the Carson River Superfund Site, west-central Nevada, USA: Interpretation of mercury speciation data in mill tailings, soils, and sediments
journal, April 1997

Lechler, Paul J.; Miller, Jerry R.; Hsu, Liang-Chi
Journal of Geochemical Exploration, Vol. 58, Issue 2-3
DOI: 10.1016/S0375-6742(96)00071-4

Speciation of Mercury and Mode of Transport from Placer Gold Mine Tailings
journal, January 2005

Slowey, Aaron J.; Rytuba, James J.; Brown, Gordon E.
Environmental Science & Technology, Vol. 39, Issue 6
DOI: 10.1021/es049113z

Anaerobic oxidation of Hg(0) and methylmercury formation by Desulfovibrio desulfuricans ND132
journal, July 2013

Colombo, Matthew J.; Ha, Juyoung; Reinfelder, John R.
Geochimica et Cosmochimica Acta, Vol. 112
DOI: 10.1016/j.gca.2013.03.001

Oxidation and methylation of dissolved elemental mercury by anaerobic bacteria
journal, August 2013

Hu, Haiyan; Lin, Hui; Zheng, Wang
Nature Geoscience, Vol. 6, Issue 9
DOI: 10.1038/ngeo1894

Unexpected Effects of Gene Deletion on Interactions of Mercury with the Methylation-Deficient Mutant Δ hgcAB
journal, April 2014

Lin, Hui; Hurt, Richard A.; Johs, Alexander
Environmental Science & Technology Letters, Vol. 1, Issue 5
DOI: 10.1021/ez500107r

Coupled Mercury–Cell Sorption, Reduction, and Oxidation on Methylmercury Production by Geobacter sulfurreducens PCA
journal, October 2014

Lin, Hui; Morrell-Falvey, Jennifer L.; Rao, Balaji
Environmental Science & Technology, Vol. 48, Issue 20
DOI: 10.1021/es502537a

Mercury reduction and complexation by natural organic matter in anoxic environments
journal, January 2011

Gu, B.; Bian, Y.; Miller, C. L.
Proceedings of the National Academy of Sciences, Vol. 108, Issue 4
DOI: 10.1073/pnas.1008747108

Mercury Reduction and Oxidation by Reduced Natural Organic Matter in Anoxic Environments
journal, November 2011

Zheng, Wang; Liang, Liyuan; Gu, Baohua
Environmental Science & Technology, Vol. 46, Issue 1
DOI: 10.1021/es203402p

Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation
journal, April 2011

Gilmour, Cynthia C.; Elias, Dwayne A.; Kucken, Amy M.
Applied and Environmental Microbiology, Vol. 77, Issue 12, p. 3938-3951
DOI: 10.1128/AEM.02993-10

Sporulation and further nutritional characteristics of Desulfotomaculum acetoxidans
journal, July 1981

Widdel, Friedrich; Pfennig, Norbert
Archives of Microbiology, Vol. 129, Issue 5
DOI: 10.1007/BF00406471

Involvement of Geobacter sulfurreducens SfrAB in acetate metabolism rather than intracellular, respiration-linked Fe(III) citrate reduction
journal, October 2007

Coppi, M. V.; O'Neil, R. A.; Leang, C.
Microbiology, Vol. 153, Issue 10
DOI: 10.1099/mic.0.2007/006478-0

A Highly Sensitive Fluorescence Probe for Fast Thiol-Quantification Assay of Glutathione Reductase
journal, May 2009

Yi, Long; Li, Heyang; Sun, Lu
Angewandte Chemie International Edition, Vol. 48, Issue 22
DOI: 10.1002/anie.200805693

High-Throughput Mass Spectrometric Analysis of Covalent Protein-Inhibitor Adducts for the Discovery of Irreversible Inhibitors: A Complete Workflow
journal, December 2015

Campuzano, Iain D. G.; San Miguel, Tisha; Rowe, Todd
Journal of Biomolecular Screening, Vol. 21, Issue 2
DOI: 10.1177/1087057115621288

The UWXAFS analysis package: philosophy and details
journal, March 1995

Stern, E. A.; Newville, M.; Ravel, B.
Physica B: Condensed Matter, Vol. 208-209
DOI: 10.1016/0921-4526(94)00826-H

ATHENA and ARTEMIS Interactive Graphical Data Analysisusing IFEFFIT
journal, January 2005

Ravel, B.; Newville, M.
Physica Scripta
DOI: 10.1238/Physica.Topical.115a01007

High- and low-affinity binding sites for Cd on the bacterial cell walls of Bacillus subtilis and Shewanella oneidensis
journal, August 2010

Mishra, Bhoopesh; Boyanov, Maxim; Bunker, Bruce A.
Geochimica et Cosmochimica Acta, Vol. 74, Issue 15
DOI: 10.1016/j.gca.2010.02.019

Thiol self-assembled monolayers on mercury surfaces: the adsorption and electrochemistry of ω-mercaptoalkanoic acids
journal, November 1999

Muskal, Nechama; Mandler, Daniel
Electrochimica Acta, Vol. 45, Issue 4-5
DOI: 10.1016/S0013-4686(99)00232-7

Studying thiol adsorption on Au, Ag and Hg surfaces by potentiometric measurements
journal, July 2003

Cohen-Atiya, Meirav; Mandler, Daniel
Journal of Electroanalytical Chemistry, Vol. 550-551
DOI: 10.1016/S0022-0728(02)01145-2

Self-assembled monolayers (SAMs) for electrochemical sensing
journal, July 2011

Mandler, Daniel; Kraus-Ophir, Shlomit
Journal of Solid State Electrochemistry, Vol. 15, Issue 7-8
DOI: 10.1007/s10008-011-1493-6

Oxidation of Dissolved Elemental Mercury by Thiol Compounds under Anoxic Conditions
journal, November 2013

Zheng, Wang; Lin, Hui; Mann, Benjamin F.
Environmental Science & Technology, Vol. 47, Issue 22
DOI: 10.1021/es402697u

Mercury Reduction and Cell-Surface Adsorption by Geobacter sulfurreducens PCA
journal, September 2013

Hu, Haiyan; Lin, Hui; Zheng, Wang
Environmental Science & Technology, Vol. 47, Issue 19
DOI: 10.1021/es400527m

The effect of natural organic matter on the adsorption of mercury to bacterial cells
journal, February 2015

Dunham-Cheatham, Sarrah; Mishra, Bhoopesh; Myneni, Satish
Geochimica et Cosmochimica Acta, Vol. 150
DOI: 10.1016/j.gca.2014.11.018

Binding of Hg ^II to High-Affinity Sites on Bacteria Inhibits Reduction to Hg ⁰ by Mixed Fe ^II/III Phases
journal, November 2011

Mishra, Bhoopesh; O’Loughlin, Edward J.; Boyanov, Maxim I.
Environmental Science & Technology, Vol. 45, Issue 22
DOI: 10.1021/es201820c

Bacterial mercury resistance from atoms to ecosystems
journal, June 2003

Barkay, Tamar; Miller, Susan M.; Summers, Anne O.
FEMS Microbiology Reviews, Vol. 27, Issue 2-3
DOI: 10.1016/S0168-6445(03)00046-9

Determination of thiol functional groups on bacteria and natural organic matter in environmental systems
journal, February 2014

Rao, Balaji; Simpson, Carolyne; Lin, Hui
Talanta, Vol. 119
DOI: 10.1016/j.talanta.2013.11.004

The impact of ionic mercury on antioxidant defenses in two mercury-sensitive anaerobic bacteria
journal, October 2013

Wang, Yingjiao; Robison, Tyler; Wiatrowski, Heather
BioMetals, Vol. 26, Issue 6
DOI: 10.1007/s10534-013-9679-2

Thioredoxin system in obligate anaerobe Desulfovibrio desulfuricans: Identification and characterization of a novel thioredoxin 2
journal, July 2006

Sarin, Ritu; Sharma, Yagya D.
Gene, Vol. 376, Issue 1
DOI: 10.1016/j.gene.2006.02.012

Response of the anaerobe Desulfovibrio vulgaris Hildenborough to oxidative conditions: proteome and transcript analysis
journal, January 2006

Fournier, Marjorie; Aubert, Corinne; Dermoun, Zorah
Biochimie, Vol. 88, Issue 1
DOI: 10.1016/j.biochi.2005.06.012

Occurrence of glutathione in bacteria
journal, March 1978

Fahey, R. C.; Brown, W. C.; Adams, W. B.
Journal of Bacteriology, Vol. 133, Issue 3
DOI: 10.1128/JB.133.3.1126-1129.1978

Relationships between Hg(ii)–S bond distance and Hg(ii) coordination in thiolates
journal, January 2008

Manceau, Alain; Nagy, Kathryn L.
Dalton Transactions, Issue 11
DOI: 10.1039/b718372k

Metallothionein-Like Multinuclear Clusters of Mercury(II) and Sulfur in Peat
journal, September 2011

Nagy, Kathryn L.; Manceau, Alain; Gasper, Jarrod D.
Environmental Science & Technology, Vol. 45, Issue 17
DOI: 10.1021/es201025v

Nuclear magnetic resonance studies of the solution chemistry of metal complexes. 25. Hg(thiol)3 complexes and HG(II)-thiol ligand exchange kinetics
journal, September 1988

Cheesman, Bruce V.; Arnold, Alan P.; Rabenstein, Dallas L.
Journal of the American Chemical Society, Vol. 110, Issue 19
DOI: 10.1021/ja00227a014

Mercury(II) Cysteine Complexes in Alkaline Aqueous Solution
journal, January 2006

Jalilehvand, Farideh; Leung, Bonnie O.; Izadifard, Maryam
Inorganic Chemistry, Vol. 45, Issue 1
DOI: 10.1021/ic0508932

Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths
journal, September 2008

Hwang, Chiachi; Wu, Weimin; Gentry, Terry J.
The ISME Journal, Vol. 3, Issue 1
DOI: 10.1038/ismej.2008.77

A Limited Microbial Consortium Is Responsible for Extended Bioreduction of Uranium in a Contaminated Aquifer
journal, July 2011

Gihring, Thomas M.; Zhang, Gengxin; Brandt, Craig C.
Applied and Environmental Microbiology, Vol. 77, Issue 17
DOI: 10.1128/AEM.00220-11

XANES Studies of Oxidation States of Sulfur in Aquatic and Soil Humic Substances
journal, January 1998

Xia, K.; Weesner, F.; Bleam, W. F.
Soil Science Society of America Journal, Vol. 62, Issue 5
DOI: 10.2136/sssaj1998.03615995006200050014x

Works referencing / citing this record:

Quantitative Proteomic Analysis of Biological Processes and Responses of the Bacterium Desulfovibrio desulfuricans ND132 upon Deletion of Its Mercury Methylation Genes
journal, August 2018

Qian, Chen; Chen, Hongmei; Johs, Alexander
PROTEOMICS, Vol. 18, Issue 17
DOI: 10.1002/pmic.201700479

Mercury isotope signatures record photic zone euxinia in the Mesoproterozoic ocean
journal, October 2018

Zheng, Wang; Gilleaudeau, Geoffrey J.; Kah, Linda C.
Proceedings of the National Academy of Sciences, Vol. 115, Issue 42
DOI: 10.1073/pnas.1721733115

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Title: Intracellular Hg(0) Oxidation in Desulfovibrio desulfuricans ND132

Abstract

Citation Formats

Mercury Speciation and Mobilization in a Wastewater-Contaminated Groundwater Plume journal, November 2013

Current Perspectives in Contaminant Hydrology and Water Resources Sustainability book, February 2013

Mercury (II) reduction and co-precipitation of metallic mercury on hydrous ferric oxide in contaminated groundwater journal, January 2016

X-ray Absorption Spectroscopic Evidence for the Complexation of Hg(II) by Reduced Sulfur in Soil Humic Substances journal, January 1999

Interactions between mercury and dissolved organic matter––a review journal, April 2004

Transport of elemental mercury in the unsaturated zone from a waste disposal site in an arid region journal, March 2008

Mercury speciation analyses in HgCl2-contaminated soils and groundwater—Implications for risk assessment and remediation strategies journal, January 2008

The Chemical Cycle and Bioaccumulation of Mercury journal, November 1998

Mechanisms Regulating Mercury Bioavailability for Methylating Microorganisms in the Aquatic Environment: A Critical Review journal, February 2013

History of mercury use and environmental contamination at the Oak Ridge Y-12 Plant journal, January 2011

Characterization of soils from an industrial complex contaminated with elemental mercury journal, August 2013

Mercury mobility at the Carson River Superfund Site, west-central Nevada, USA: Interpretation of mercury speciation data in mill tailings, soils, and sediments journal, April 1997

Speciation of Mercury and Mode of Transport from Placer Gold Mine Tailings journal, January 2005

Anaerobic oxidation of Hg(0) and methylmercury formation by Desulfovibrio desulfuricans ND132 journal, July 2013

Oxidation and methylation of dissolved elemental mercury by anaerobic bacteria journal, August 2013

Unexpected Effects of Gene Deletion on Interactions of Mercury with the Methylation-Deficient Mutant Δ hgcAB journal, April 2014

Coupled Mercury–Cell Sorption, Reduction, and Oxidation on Methylmercury Production by Geobacter sulfurreducens PCA journal, October 2014

Mercury reduction and complexation by natural organic matter in anoxic environments journal, January 2011

Mercury Reduction and Oxidation by Reduced Natural Organic Matter in Anoxic Environments journal, November 2011

Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation journal, April 2011

Sporulation and further nutritional characteristics of Desulfotomaculum acetoxidans journal, July 1981

Involvement of Geobacter sulfurreducens SfrAB in acetate metabolism rather than intracellular, respiration-linked Fe(III) citrate reduction journal, October 2007

A Highly Sensitive Fluorescence Probe for Fast Thiol-Quantification Assay of Glutathione Reductase journal, May 2009

High-Throughput Mass Spectrometric Analysis of Covalent Protein-Inhibitor Adducts for the Discovery of Irreversible Inhibitors: A Complete Workflow journal, December 2015

The UWXAFS analysis package: philosophy and details journal, March 1995

ATHENA and ARTEMIS Interactive Graphical Data Analysisusing IFEFFIT journal, January 2005

High- and low-affinity binding sites for Cd on the bacterial cell walls of Bacillus subtilis and Shewanella oneidensis journal, August 2010

Thiol self-assembled monolayers on mercury surfaces: the adsorption and electrochemistry of ω-mercaptoalkanoic acids journal, November 1999

Studying thiol adsorption on Au, Ag and Hg surfaces by potentiometric measurements journal, July 2003

Self-assembled monolayers (SAMs) for electrochemical sensing journal, July 2011

Oxidation of Dissolved Elemental Mercury by Thiol Compounds under Anoxic Conditions journal, November 2013

Mercury Reduction and Cell-Surface Adsorption by Geobacter sulfurreducens PCA journal, September 2013

The effect of natural organic matter on the adsorption of mercury to bacterial cells journal, February 2015

Binding of Hg II to High-Affinity Sites on Bacteria Inhibits Reduction to Hg 0 by Mixed Fe II/III Phases journal, November 2011

Bacterial mercury resistance from atoms to ecosystems journal, June 2003

Determination of thiol functional groups on bacteria and natural organic matter in environmental systems journal, February 2014

The impact of ionic mercury on antioxidant defenses in two mercury-sensitive anaerobic bacteria journal, October 2013

Thioredoxin system in obligate anaerobe Desulfovibrio desulfuricans: Identification and characterization of a novel thioredoxin 2 journal, July 2006

Response of the anaerobe Desulfovibrio vulgaris Hildenborough to oxidative conditions: proteome and transcript analysis journal, January 2006

Occurrence of glutathione in bacteria journal, March 1978

Relationships between Hg(ii)–S bond distance and Hg(ii) coordination in thiolates journal, January 2008

Metallothionein-Like Multinuclear Clusters of Mercury(II) and Sulfur in Peat journal, September 2011

Nuclear magnetic resonance studies of the solution chemistry of metal complexes. 25. Hg(thiol)3 complexes and HG(II)-thiol ligand exchange kinetics journal, September 1988

Mercury(II) Cysteine Complexes in Alkaline Aqueous Solution journal, January 2006

Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths journal, September 2008

A Limited Microbial Consortium Is Responsible for Extended Bioreduction of Uranium in a Contaminated Aquifer journal, July 2011

XANES Studies of Oxidation States of Sulfur in Aquatic and Soil Humic Substances journal, January 1998

Quantitative Proteomic Analysis of Biological Processes and Responses of the Bacterium Desulfovibrio desulfuricans ND132 upon Deletion of Its Mercury Methylation Genes journal, August 2018

Mercury isotope signatures record photic zone euxinia in the Mesoproterozoic ocean journal, October 2018

Mercury Speciation and Mobilization in a Wastewater-Contaminated Groundwater Plume
journal, November 2013

Current Perspectives in Contaminant Hydrology and Water Resources Sustainability
book, February 2013

Mercury (II) reduction and co-precipitation of metallic mercury on hydrous ferric oxide in contaminated groundwater
journal, January 2016

X-ray Absorption Spectroscopic Evidence for the Complexation of Hg(II) by Reduced Sulfur in Soil Humic Substances
journal, January 1999

Interactions between mercury and dissolved organic matter––a review
journal, April 2004

Transport of elemental mercury in the unsaturated zone from a waste disposal site in an arid region
journal, March 2008

Mercury speciation analyses in HgCl2-contaminated soils and groundwater—Implications for risk assessment and remediation strategies
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The Chemical Cycle and Bioaccumulation of Mercury
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History of mercury use and environmental contamination at the Oak Ridge Y-12 Plant
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Characterization of soils from an industrial complex contaminated with elemental mercury
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Mercury mobility at the Carson River Superfund Site, west-central Nevada, USA: Interpretation of mercury speciation data in mill tailings, soils, and sediments
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Speciation of Mercury and Mode of Transport from Placer Gold Mine Tailings
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Anaerobic oxidation of Hg(0) and methylmercury formation by Desulfovibrio desulfuricans ND132
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Oxidation and methylation of dissolved elemental mercury by anaerobic bacteria
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Unexpected Effects of Gene Deletion on Interactions of Mercury with the Methylation-Deficient Mutant Δ hgcAB
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Coupled Mercury–Cell Sorption, Reduction, and Oxidation on Methylmercury Production by Geobacter sulfurreducens PCA
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Mercury reduction and complexation by natural organic matter in anoxic environments
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Mercury Reduction and Oxidation by Reduced Natural Organic Matter in Anoxic Environments
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Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation
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Sporulation and further nutritional characteristics of Desulfotomaculum acetoxidans
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Involvement of Geobacter sulfurreducens SfrAB in acetate metabolism rather than intracellular, respiration-linked Fe(III) citrate reduction
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A Highly Sensitive Fluorescence Probe for Fast Thiol-Quantification Assay of Glutathione Reductase
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High-Throughput Mass Spectrometric Analysis of Covalent Protein-Inhibitor Adducts for the Discovery of Irreversible Inhibitors: A Complete Workflow
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The UWXAFS analysis package: philosophy and details
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ATHENA and ARTEMIS Interactive Graphical Data Analysisusing IFEFFIT
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High- and low-affinity binding sites for Cd on the bacterial cell walls of Bacillus subtilis and Shewanella oneidensis
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Thiol self-assembled monolayers on mercury surfaces: the adsorption and electrochemistry of ω-mercaptoalkanoic acids
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Studying thiol adsorption on Au, Ag and Hg surfaces by potentiometric measurements
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Self-assembled monolayers (SAMs) for electrochemical sensing
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Oxidation of Dissolved Elemental Mercury by Thiol Compounds under Anoxic Conditions
journal, November 2013

Mercury Reduction and Cell-Surface Adsorption by Geobacter sulfurreducens PCA
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The effect of natural organic matter on the adsorption of mercury to bacterial cells
journal, February 2015

Binding of Hg ^II to High-Affinity Sites on Bacteria Inhibits Reduction to Hg ⁰ by Mixed Fe ^II/III Phases
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Bacterial mercury resistance from atoms to ecosystems
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Determination of thiol functional groups on bacteria and natural organic matter in environmental systems
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The impact of ionic mercury on antioxidant defenses in two mercury-sensitive anaerobic bacteria
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Thioredoxin system in obligate anaerobe Desulfovibrio desulfuricans: Identification and characterization of a novel thioredoxin 2
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Response of the anaerobe Desulfovibrio vulgaris Hildenborough to oxidative conditions: proteome and transcript analysis
journal, January 2006

Occurrence of glutathione in bacteria
journal, March 1978

Relationships between Hg(ii)–S bond distance and Hg(ii) coordination in thiolates
journal, January 2008

Metallothionein-Like Multinuclear Clusters of Mercury(II) and Sulfur in Peat
journal, September 2011

Nuclear magnetic resonance studies of the solution chemistry of metal complexes. 25. Hg(thiol)3 complexes and HG(II)-thiol ligand exchange kinetics
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Mercury(II) Cysteine Complexes in Alkaline Aqueous Solution
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Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths
journal, September 2008

A Limited Microbial Consortium Is Responsible for Extended Bioreduction of Uranium in a Contaminated Aquifer
journal, July 2011

XANES Studies of Oxidation States of Sulfur in Aquatic and Soil Humic Substances
journal, January 1998

Quantitative Proteomic Analysis of Biological Processes and Responses of the Bacterium Desulfovibrio desulfuricans ND132 upon Deletion of Its Mercury Methylation Genes
journal, August 2018

Mercury isotope signatures record photic zone euxinia in the Mesoproterozoic ocean
journal, October 2018