Sequence-based analysis of the Vitis vinifera L. cv Cabernet Sauvignon grape must mycobiome in three South African vineyards employing distinct agronomic systems
Abstract
Recent microbiomic research of agricultural habitats has highlighted tremendous microbial biodiversity associated with such ecosystems. In addition, data generated in vineyards have furthermore highlighted significant regional differences in vineyard biodiversity, hinting at the possibility that such differences might be responsible for regional differences in wine style and character, a hypothesis referred to as "microbial terroir." The current study further contributes to this body of work by comparing the mycobiome associated with South African (SA) Cabernet Sauvignon grapes in three neighboring vineyards that employ different agronomic approaches, and comparing the outcome with similar data sets from Californian vineyards. The aim of this study was to fully characterize the mycobiomes associated with the grapes from these vineyards. The data revealed approximately 10 times more fungal diversity than what is typically retrieved from culture-based studies. The Biodynamic vineyard was found to harbor a more diverse fungal community (H = 2.6) than the conventional (H = 2.1) and integrated (H = 1.8) vineyards. The data show that ascomycota are the most abundant phylum in the three vineyards, with Aureobasidium pullulans and its close relative Kabatiella microsticta being the most dominant fungi. This is the first report to reveal a high incidence of K. microstictamore »
- Authors:
-
- Stellenbosch Univ., Stellenbosch (South Africa)
- Stellenbosch Univ., Stellenbosch (South Africa); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Laboratory Directed Research and Development (LDRD) Program
- OSTI Identifier:
- 1327739
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 6; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; wine yeasts; next-generation sequencing; grapevine mycobiome; microbial diversity; microbial terroir
Citation Formats
Setati, Mathabatha E., Jacobson, Daniel, and Bauer, Florian F. Sequence-based analysis of the Vitis vinifera L. cv Cabernet Sauvignon grape must mycobiome in three South African vineyards employing distinct agronomic systems. United States: N. p., 2015.
Web. doi:10.3389/fmicb.2015.01358.
Setati, Mathabatha E., Jacobson, Daniel, & Bauer, Florian F. Sequence-based analysis of the Vitis vinifera L. cv Cabernet Sauvignon grape must mycobiome in three South African vineyards employing distinct agronomic systems. United States. https://doi.org/10.3389/fmicb.2015.01358
Setati, Mathabatha E., Jacobson, Daniel, and Bauer, Florian F. Mon .
"Sequence-based analysis of the Vitis vinifera L. cv Cabernet Sauvignon grape must mycobiome in three South African vineyards employing distinct agronomic systems". United States. https://doi.org/10.3389/fmicb.2015.01358. https://www.osti.gov/servlets/purl/1327739.
@article{osti_1327739,
title = {Sequence-based analysis of the Vitis vinifera L. cv Cabernet Sauvignon grape must mycobiome in three South African vineyards employing distinct agronomic systems},
author = {Setati, Mathabatha E. and Jacobson, Daniel and Bauer, Florian F.},
abstractNote = {Recent microbiomic research of agricultural habitats has highlighted tremendous microbial biodiversity associated with such ecosystems. In addition, data generated in vineyards have furthermore highlighted significant regional differences in vineyard biodiversity, hinting at the possibility that such differences might be responsible for regional differences in wine style and character, a hypothesis referred to as "microbial terroir." The current study further contributes to this body of work by comparing the mycobiome associated with South African (SA) Cabernet Sauvignon grapes in three neighboring vineyards that employ different agronomic approaches, and comparing the outcome with similar data sets from Californian vineyards. The aim of this study was to fully characterize the mycobiomes associated with the grapes from these vineyards. The data revealed approximately 10 times more fungal diversity than what is typically retrieved from culture-based studies. The Biodynamic vineyard was found to harbor a more diverse fungal community (H = 2.6) than the conventional (H = 2.1) and integrated (H = 1.8) vineyards. The data show that ascomycota are the most abundant phylum in the three vineyards, with Aureobasidium pullulans and its close relative Kabatiella microsticta being the most dominant fungi. This is the first report to reveal a high incidence of K. microsticta in the grape/wine ecosystem. Different common wine yeast species, such as Metschnikowia pulcherrima and Starmerella bacillaris dominated the mycobiome in the three vineyards. The data show that the filamentous fungi are the most abundant community in grape must although they are not regarded as relevant during wine fermentation. Comparison of metagenomic datasets from the three SA vineyards and previously published data from Californian vineyards revealed only 25% of the fungi in the SA dataset was also present in the Californian dataset, with greater variation evident amongst ubiquitous epiphytic fungi.},
doi = {10.3389/fmicb.2015.01358},
journal = {Frontiers in Microbiology},
number = ,
volume = 6,
place = {United States},
year = {Mon Nov 30 00:00:00 EST 2015},
month = {Mon Nov 30 00:00:00 EST 2015}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 54 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Occurrence of Erysiphe necator Chasmothecia and Their Natural Parasitism by Ampelomyces quisqualis
journal, June 2009
- Angeli, Dario; Pellegrini, Elisabetta; Pertot, Ilaria
- Phytopathology, Vol. 99, Issue 6
The microbial ecology of wine grape berries
journal, February 2012
- Barata, A.; Malfeito-Ferreira, M.; Loureiro, V.
- International Journal of Food Microbiology, Vol. 153, Issue 3
Improved Selection of Internal Transcribed Spacer-Specific Primers Enables Quantitative, Ultra-High-Throughput Profiling of Fungal Communities
journal, February 2013
- Bokulich, Nicholas A.; Mills, David A.
- Applied and Environmental Microbiology, Vol. 79, Issue 8
PNAS Plus: From the Cover: Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate
journal, November 2013
- Bokulich, N. A.; Thorngate, J. H.; Richardson, P. M.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 1
SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data
journal, September 2010
- Cox, Murray P.; Peterson, Daniel A.; Biggs, Patrick J.
- BMC Bioinformatics, Vol. 11, Issue 1
High-throughput sequencing of amplicons for monitoring yeast biodiversity in must and during alcoholic fermentation
journal, March 2014
- David, Vanessa; Terrat, Sébastien; Herzine, Khaled
- Journal of Industrial Microbiology & Biotechnology, Vol. 41, Issue 5
PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes
journal, September 2011
- Diguta, C. F.; Vincent, B.; Guilloux-Benatier, M.
- Food Microbiology, Vol. 28, Issue 6
Incidence, Aggressiveness and In Planta Interactions of Botrytis cinerea and other Filamentous Fungi Quiescent in Grape Berries and Dormant Buds in Central Washington State
journal, August 2002
- Dugan, F. M.; Lupien, S. L.; Grove, G. G.
- Journal of Phytopathology, Vol. 150, Issue 7
Search and clustering orders of magnitude faster than BLAST
journal, August 2010
- Edgar, Robert C.
- Bioinformatics, Vol. 26, Issue 19, p. 2460-2461
Partial Control of Grape Powdery Mildew by the Mycoparasite Ampelomyces quisqualis
journal, January 1995
- Falk, Stuart P.
- Plant Disease, Vol. 79, Issue 5
Systematic artifacts in metagenomes from complex microbial communities
journal, July 2009
- Gomez-Alvarez, Vicente; Teal, Tracy K.; Schmidt, Thomas M.
- The ISME Journal, Vol. 3, Issue 11
The endophytic mycota associated with Vitis vinifera in central Spain
journal, November 2010
- González, Vicente; Tello, María Luisa
- Fungal Diversity, Vol. 47, Issue 1
Analysis of several methods for the extraction of high quality DNA from acetic acid bacteria in wine and vinegar for characterization by PCR-based methods
journal, December 2008
- Jara, C.; Mateo, E.; Guillamón, J. M.
- International Journal of Food Microbiology, Vol. 128, Issue 2
Fungi of Grapes
book, January 2009
- Kassemeyer, Hanns-Heinz; Berkelmann-Löhnertz, Beate
- Biology of Microorganisms on Grapes, in Must and in Wine
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
journal, January 2009
- Langmead, Ben; Trapnell, Cole; Pop, Mihai
- Genome Biology, Vol. 10, Issue 3
Influence of the farming system on the epiphytic yeasts and yeast-like fungi colonizing grape berries during the ripening process
journal, May 2014
- Martins, Guilherme; Vallance, Jessica; Mercier, Anne
- International Journal of Food Microbiology, Vol. 177
The metagenomics RAST server – a public resource for the automatic phylogenetic and functional analysis of metagenomes
journal, September 2008
- Meyer, F.; Paarmann, D.; D'Souza, M.
- BMC Bioinformatics, Vol. 9, Issue 1
Presence of non-Saccharomyces yeasts in cellar equipment and grape juice during harvest time
journal, December 2010
- Ocón, E.; Gutiérrez, A. R.; Garijo, P.
- Food Microbiology, Vol. 27, Issue 8
Unravelling the Diversity of Grapevine Microbiome
journal, January 2014
- Pinto, Cátia; Pinho, Diogo; Sousa, Susana
- PLoS ONE, Vol. 9, Issue 1
Application and evaluation of denaturing gradient gel electrophoresis to analyse the yeast ecology of wine grapes
journal, September 2004
- Prakitchaiwattana, C.; Fleet, G.; Heard, G.
- FEMS Yeast Research, Vol. 4, Issue 8
Understanding the microbial ecosystem on the grape berry surface through numeration and identification of yeast and bacteria
journal, October 2005
- Renouf, Vincent; Claisse, Olivier; Lonvaud-Funel, Aline
- Australian Journal of Grape and Wine Research, Vol. 11, Issue 3
Inventory and monitoring of wine microbial consortia
journal, May 2007
- Renouf, Vincent; Claisse, Olivier; Lonvaud-Funel, Aline
- Applied Microbiology and Biotechnology, Vol. 75, Issue 1
Non-Botrytis grape-rotting fungi responsible for earthy and moldy off-flavors and mycotoxins
journal, April 2014
- Rousseaux, Sandrine; Diguta, Camelia Filofteia; Radoï-Matei, Florentina
- Food Microbiology, Vol. 38
The Vineyard Yeast Microbiome, a Mixed Model Microbial Map
journal, December 2012
- Setati, Mathabatha Evodia; Jacobson, Daniel; Andong, Ursula-Claire
- PLoS ONE, Vol. 7, Issue 12
Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks
journal, November 2003
- Shannon, P.
- Genome Research, Vol. 13, Issue 11
Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact
journal, May 2007
- Slippers, Bernard; Wingfield, Michael J.
- Fungal Biology Reviews, Vol. 21, Issue 2-3
Fungi occurring on Proteaceae: new anamorphs for Teratosphaeria, Mycosphaerella and Lembosia, and other fungi associated with leaf spots and cankers of proteaceous hosts
journal, May 2000
- Taylor, J. E.; Crous, P. W.
- Mycological Research, Vol. 104, Issue 5
Pyrosequencing reveals regional differences in fruit-associated fungal communities: Vine fungi biogeography by pyrosequencing
journal, April 2014
- Taylor, Michael W.; Tsai, Peter; Anfang, Nicole
- Environmental Microbiology, Vol. 16, Issue 9
Molecular identification and osmotolerant profile of wine yeasts that ferment a high sugar grape must
journal, April 2009
- Tofalo, Rosanna; Chaves-López, Clemencia; Di Fabio, Federico
- International Journal of Food Microbiology, Vol. 130, Issue 3
Pathogenicity of Botryosphaeriaceae Species Isolated from Grapevine Cankers in California
journal, June 2009
- Úrbez-Torres, J. R.; Gubler, W. D.
- Plant Disease, Vol. 93, Issue 6
DNA Phylogeny, Morphology and Pathogenicity of Botryosphaeria Species on Grapevines
journal, July 2004
- van Niekerk, Jan M.; Crous, Pedro W.; Groenewald, J. Z. (Ewald)
- Mycologia, Vol. 96, Issue 4
Species of Botryosphaeriaceae involved in grapevine dieback in China
journal, July 2013
- Yan, Ji-Ye; Xie, Yue; Zhang, Wei
- Fungal Diversity, Vol. 61, Issue 1
Redefinition of Aureobasidium pullulans and its varieties
journal, January 2008
- Zalar, P.; Gostinčar, C.; de Hoog, G. S.
- Studies in Mycology, Vol. 61
High-throughput sequencing of amplicons for monitoring yeast biodiversity in must and during alcoholic fermentation
journal, March 2014
- David, Vanessa; Terrat, Sébastien; Herzine, Khaled
- Journal of Industrial Microbiology & Biotechnology, Vol. 41, Issue 5
The endophytic mycota associated with Vitis vinifera in central Spain
journal, November 2010
- González, Vicente; Tello, María Luisa
- Fungal Diversity, Vol. 47, Issue 1
Species of Botryosphaeriaceae involved in grapevine dieback in China
journal, July 2013
- Yan, Ji-Ye; Xie, Yue; Zhang, Wei
- Fungal Diversity, Vol. 61, Issue 1
Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact
journal, May 2007
- Slippers, Bernard; Wingfield, Michael J.
- Fungal Biology Reviews, Vol. 21, Issue 2-3
Presence of non-Saccharomyces yeasts in cellar equipment and grape juice during harvest time
journal, December 2010
- Ocón, E.; Gutiérrez, A. R.; Garijo, P.
- Food Microbiology, Vol. 27, Issue 8
PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes
journal, September 2011
- Diguta, C. F.; Vincent, B.; Guilloux-Benatier, M.
- Food Microbiology, Vol. 28, Issue 6
Non-Botrytis grape-rotting fungi responsible for earthy and moldy off-flavors and mycotoxins
journal, April 2014
- Rousseaux, Sandrine; Diguta, Camelia Filofteia; Radoï-Matei, Florentina
- Food Microbiology, Vol. 38
Molecular identification and osmotolerant profile of wine yeasts that ferment a high sugar grape must
journal, April 2009
- Tofalo, Rosanna; Chaves-López, Clemencia; Di Fabio, Federico
- International Journal of Food Microbiology, Vol. 130, Issue 3
The microbial ecology of wine grape berries
journal, February 2012
- Barata, A.; Malfeito-Ferreira, M.; Loureiro, V.
- International Journal of Food Microbiology, Vol. 153, Issue 3
Systematic artifacts in metagenomes from complex microbial communities
journal, July 2009
- Gomez-Alvarez, Vicente; Teal, Tracy K.; Schmidt, Thomas M.
- The ISME Journal, Vol. 3, Issue 11
Comparative transcriptomic analysis reveals conserved programmes underpinning organogenesis and reproduction in land plants
journal, July 2021
- Julca, Irene; Ferrari, Camilla; Flores-Tornero, María
- Nature Plants, Vol. 7, Issue 8
Incidence, Aggressiveness and In Planta Interactions of Botrytis cinerea and other Filamentous Fungi Quiescent in Grape Berries and Dormant Buds in Central Washington State
journal, August 2002
- Dugan, F. M.; Lupien, S. L.; Grove, G. G.
- Journal of Phytopathology, Vol. 150, Issue 7
PNAS Plus: From the Cover: Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate
journal, November 2013
- Bokulich, N. A.; Thorngate, J. H.; Richardson, P. M.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 1
Search and clustering orders of magnitude faster than BLAST
journal, August 2010
- Edgar, Robert C.
- Bioinformatics, Vol. 26, Issue 19, p. 2460-2461
DNA-Dependent Detection of the Grapevine Fungal Endophytes Aureobasidium pullulans and Epicoccum nigrum
journal, October 2009
- Martini, M.; Musetti, R.; Grisan, S.
- Plant Disease, Vol. 93, Issue 10
Pyrosequencing reveals regional differences in fruit-associated fungal communities: Vine fungi biogeography by pyrosequencing
journal, April 2014
- Taylor, Michael W.; Tsai, Peter; Anfang, Nicole
- Environmental Microbiology, Vol. 16, Issue 9
Understanding the microbial ecosystem on the grape berry surface through numeration and identification of yeast and bacteria
journal, October 2005
- Renouf, Vincent; Claisse, Olivier; Lonvaud-Funel, Aline
- Australian Journal of Grape and Wine Research, Vol. 11, Issue 3
Fungal Endophytic Communities in Grapevines (Vitis vinifera L.) Respond to Crop Management
journal, April 2012
- Pancher, Michael; Ceol, Marco; Corneo, Paola Elisa
- Applied and Environmental Microbiology, Vol. 78, Issue 12
SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data
journal, September 2010
- Cox, Murray P.; Peterson, Daniel A.; Biggs, Patrick J.
- BMC Bioinformatics, Vol. 11, Issue 1
The metagenomics RAST server – a public resource for the automatic phylogenetic and functional analysis of metagenomes
journal, September 2008
- Meyer, F.; Paarmann, D.; D'Souza, M.
- BMC Bioinformatics, Vol. 9, Issue 1
The Vineyard Yeast Microbiome, a Mixed Model Microbial Map
journal, December 2012
- Setati, Mathabatha Evodia; Jacobson, Daniel; Andong, Ursula-Claire
- PLoS ONE, Vol. 7, Issue 12
Unravelling the Diversity of Grapevine Microbiome
journal, January 2014
- Pinto, Cátia; Pinho, Diogo; Sousa, Susana
- PLoS ONE, Vol. 9, Issue 1
The Diversity and Dynamics of Indigenous Yeast Communities in Grape Must from Vineyards Employing Different Agronomic Practices and their Influence on Wine Fermentation
journal, September 2016
- Bagheri, B.; Bauer, F. F.; Setati, M. E.
- South African Journal of Enology and Viticulture, Vol. 36, Issue 2
DNA Phylogeny, Morphology and Pathogenicity of Botryosphaeria Species on Grapevines
journal, July 2004
- van Niekerk, Jan M.; Crous, Pedro W.; Groenewald, J. Z. (Ewald)
- Mycologia, Vol. 96, Issue 4
Redefinition of Aureobasidium pullulans and its varieties
journal, January 2008
- Zalar, P.; Gostinčar, C.; de Hoog, G. S.
- Studies in Mycology, Vol. 61
Works referencing / citing this record:
Characterisation of the enzymatic properties of MpAPr1, an aspartic protease secreted by the wine yeast Metschnikowia pulcherrima: Characterisation of the wine yeast protease MpAPr1
journal, February 2017
- Theron, Louwrens Wiid; Bely, Marina; Divol, Benoit
- Journal of the Science of Food and Agriculture, Vol. 97, Issue 11
Direct comparison of culture-dependent and culture-independent molecular approaches reveal the diversity of fungal endophytic communities in stems of grapevine (Vitis vinifera)
journal, April 2018
- Dissanayake, Asha J.; Purahong, Witoon; Wubet, Tesfaye
- Fungal Diversity, Vol. 90, Issue 1
Fermentation performances and aroma production of non-conventional wine yeasts are influenced by nitrogen preferences
journal, May 2018
- Rollero, Stéphanie; Bloem, Audrey; Ortiz-Julien, Anne
- FEMS Yeast Research, Vol. 18, Issue 5
Profiling grapevine trunk pathogens in planta: a case for community-targeted DNA metabarcoding
journal, December 2018
- Morales-Cruz, Abraham; Figueroa-Balderas, Rosa; García, Jadran F.
- BMC Microbiology, Vol. 18, Issue 1
The epiphytic microbiota of sour rot-affected grapes differs minimally from that of healthy grapes, indicating causal organisms are already present on healthy berries
journal, March 2019
- Hall, Megan E.; O’Bryon, Isabelle; Wilcox, Wayne F.
- PLOS ONE, Vol. 14, Issue 3
Overwintering of Vineyard Yeasts: Survival of Interacting Yeast Communities in Grapes Mummified on Vines
journal, February 2016
- Sipiczki, Matthias
- Frontiers in Microbiology, Vol. 7
Genetic Polymorphism in Wine Yeasts: Mechanisms and Methods for Its Detection
journal, May 2017
- Guillamón, José M.; Barrio, Eladio
- Frontiers in Microbiology, Vol. 8
The Grapevine and Wine Microbiome: Insights from High-Throughput Amplicon Sequencing
journal, May 2017
- Morgan, Horatio H.; du Toit, Maret; Setati, Mathabatha E.
- Frontiers in Microbiology, Vol. 8
The Impact of Saccharomyces cerevisiae on a Wine Yeast Consortium in Natural and Inoculated Fermentations
journal, October 2017
- Bagheri, Bahareh; Bauer, Florian F.; Setati, Mathabatha E.
- Frontiers in Microbiology, Vol. 8
Fungal Endophytic Communities of Two Wild Rosa Varieties With Different Powdery Mildew Susceptibilities
journal, October 2018
- Zhao, Yi; Xiong, Zhi; Wu, Guangli
- Frontiers in Microbiology, Vol. 9
The Multiple and Versatile Roles of Aureobasidium pullulans in the Vitivinicultural Sector
journal, October 2018
- Bozoudi, Despina; Tsaltas, Dimitrios
- Fermentation, Vol. 4, Issue 4
Geographical Origin Has a Greater Impact on Grape Berry Fungal Community than Grape Variety and Maturation State
journal, December 2019
- Kioroglou, Dimitrios; Kraeva-Deloire, Elena; Schmidtke, Leigh M.
- Microorganisms, Vol. 7, Issue 12
Profiling grapevine trunk pathogens in planta: A case for community-targeted DNA metabarcoding
journal, September 2018
- Morales-Cruz, Abraham; Figueroa-Balderas, Rosa; Garcia, Jadran f.
- BMC Microbiology
Profiling grapevine trunk pathogens in planta: a case for community-targeted DNA metabarcoding
journal, December 2018
- Morales-Cruz, Abraham; Figueroa-Balderas, Rosa; García, Jadran F.
- BMC Microbiology, Vol. 18, Issue 1
The epiphytic microbiota of sour rot-affected grapes differs minimally from that of healthy grapes, indicating causal organisms are already present on healthy berries
journal, March 2019
- Hall, Megan E.; O’Bryon, Isabelle; Wilcox, Wayne F.
- PLOS ONE, Vol. 14, Issue 3
Yeast Biodiversity from DOQ Priorat Uninoculated Fermentations
journal, June 2016
- Padilla, Beatriz; García-Fernández, David; González, Beatriz
- Frontiers in Microbiology, Vol. 7
Isolation and Identification of the Indigenous Yeast Population during Spontaneous Fermentation of Isabella (Vitis labrusca L.) Grape Must
journal, March 2017
- Raymond Eder, María L.; Reynoso, Cristina; Lauret, Santiago C.
- Frontiers in Microbiology, Vol. 8
Genetic Polymorphism in Wine Yeasts: Mechanisms and Methods for Its Detection
journal, May 2017
- Guillamón, José M.; Barrio, Eladio
- Frontiers in Microbiology, Vol. 8
The Grapevine and Wine Microbiome: Insights from High-Throughput Amplicon Sequencing
journal, May 2017
- Morgan, Horatio H.; du Toit, Maret; Setati, Mathabatha E.
- Frontiers in Microbiology, Vol. 8
Metagenomic Approaches to Investigate the Contribution of the Vineyard Environment to the Quality of Wine Fermentation: Potentials and Difficulties
journal, May 2018
- Stefanini, Irene; Cavalieri, Duccio
- Frontiers in Microbiology, Vol. 9
Geographical Origin Has a Greater Impact on Grape Berry Fungal Community than Grape Variety and Maturation State
journal, December 2019
- Kioroglou, Dimitrios; Kraeva-Deloire, Elena; Schmidtke, Leigh M.
- Microorganisms, Vol. 7, Issue 12
Fungal Diversity of Deteriorated Sparkling Wine and Cork Stoppers in Catalonia, Spain
journal, December 2019
- Rodríguez-Andrade, Ernesto; Stchigel, Alberto M.; Guarro, Josep
- Microorganisms, Vol. 8, Issue 1
Grape Microbiome: Potential and Opportunities as a Source of Starter Cultures
book, October 2016
- Bozoudi, Despina; Tsaltas, Dimitris
- Grape and Wine Biotechnology
Effects of agricultural management on phyllosphere fungal diversity in vineyards and the association with adjacent native forests
journal, October 2018
- Castañeda, Luis E.; Miura, Toshiko; Sánchez, Roland
- PeerJ, Vol. 6