Systems biology-guided biodesign of consolidated lignin conversion
Abstract
Lignin is the second most abundant biopolymer on the earth, yet its utilization for fungible products is complicated by its recalcitrant nature and remains a major challenge for sustainable lignocellulosic biorefineries. In this study, we used a systems biology approach to reveal the carbon utilization pattern and lignin degradation mechanisms in a unique lignin-utilizing Pseudomonas putida strain (A514). The mechanistic study further guided the design of three functional modules to enable a consolidated lignin bioconversion route. First, P. putida A514 mobilized a dye peroxidase-based enzymatic system for lignin depolymerization. This system could be enhanced by overexpressing a secreted multifunctional dye peroxidase to promote a two-fold enhancement of cell growth on insoluble kraft lignin. Second, A514 employed a variety of peripheral and central catabolism pathways to metabolize aromatic compounds, which can be optimized by overexpressing key enzymes. Third, the β-oxidation of fatty acid was up-regulated, whereas fatty acid synthesis was down-regulated when A514 was grown on lignin and vanillic acid. Therefore, the functional module for polyhydroxyalkanoate (PHA) production was designed to rechannel β-oxidation products. As a result, PHA content reached 73% per cell dry weight (CDW). Further integrating the three functional modules enhanced the production of PHA from kraft lignin andmore »
- Authors:
-
- Texas A & M Univ., College Station, TX (United States); Zhejiang Univ., Hangzhou (China)
- Texas A & M Univ., College Station, TX (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Michigan State Univ., East Lansing, MI (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1326560
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Green Chemistry
- Additional Journal Information:
- Journal Name: Green Chemistry; Journal ID: ISSN 1463-9262
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Lin, Lu, Cheng, Yanbing, Pu, Yunqiao, Sun, Su, Li, Xiao, Jin, Mingjie, Pierson, Elizabeth A., Gross, Dennis C., Dale, Bruce E., Dai, Susie Y., Ragauskas, Arthur J., and Yuan, Joshua S. Systems biology-guided biodesign of consolidated lignin conversion. United States: N. p., 2016.
Web. doi:10.1039/C6GC01131D.
Lin, Lu, Cheng, Yanbing, Pu, Yunqiao, Sun, Su, Li, Xiao, Jin, Mingjie, Pierson, Elizabeth A., Gross, Dennis C., Dale, Bruce E., Dai, Susie Y., Ragauskas, Arthur J., & Yuan, Joshua S. Systems biology-guided biodesign of consolidated lignin conversion. United States. https://doi.org/10.1039/C6GC01131D
Lin, Lu, Cheng, Yanbing, Pu, Yunqiao, Sun, Su, Li, Xiao, Jin, Mingjie, Pierson, Elizabeth A., Gross, Dennis C., Dale, Bruce E., Dai, Susie Y., Ragauskas, Arthur J., and Yuan, Joshua S. Tue .
"Systems biology-guided biodesign of consolidated lignin conversion". United States. https://doi.org/10.1039/C6GC01131D. https://www.osti.gov/servlets/purl/1326560.
@article{osti_1326560,
title = {Systems biology-guided biodesign of consolidated lignin conversion},
author = {Lin, Lu and Cheng, Yanbing and Pu, Yunqiao and Sun, Su and Li, Xiao and Jin, Mingjie and Pierson, Elizabeth A. and Gross, Dennis C. and Dale, Bruce E. and Dai, Susie Y. and Ragauskas, Arthur J. and Yuan, Joshua S.},
abstractNote = {Lignin is the second most abundant biopolymer on the earth, yet its utilization for fungible products is complicated by its recalcitrant nature and remains a major challenge for sustainable lignocellulosic biorefineries. In this study, we used a systems biology approach to reveal the carbon utilization pattern and lignin degradation mechanisms in a unique lignin-utilizing Pseudomonas putida strain (A514). The mechanistic study further guided the design of three functional modules to enable a consolidated lignin bioconversion route. First, P. putida A514 mobilized a dye peroxidase-based enzymatic system for lignin depolymerization. This system could be enhanced by overexpressing a secreted multifunctional dye peroxidase to promote a two-fold enhancement of cell growth on insoluble kraft lignin. Second, A514 employed a variety of peripheral and central catabolism pathways to metabolize aromatic compounds, which can be optimized by overexpressing key enzymes. Third, the β-oxidation of fatty acid was up-regulated, whereas fatty acid synthesis was down-regulated when A514 was grown on lignin and vanillic acid. Therefore, the functional module for polyhydroxyalkanoate (PHA) production was designed to rechannel β-oxidation products. As a result, PHA content reached 73% per cell dry weight (CDW). Further integrating the three functional modules enhanced the production of PHA from kraft lignin and biorefinery waste. Furthermore, this study elucidated lignin conversion mechanisms in bacteria with potential industrial implications and laid out the concept for engineering a consolidated lignin conversion route.},
doi = {10.1039/C6GC01131D},
journal = {Green Chemistry},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 12 00:00:00 EDT 2016},
month = {Tue Jul 12 00:00:00 EDT 2016}
}
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