1,2-Propanediol Dehydration in Roseburia inulinivorans: Structural Basis for Substrate and Enantiomer Selectivity
Abstract
Glycyl radical enzymes (GREs) represent a diverse superfamily of enzymes that utilize a radical mechanism to catalyze difficult, but often essential, chemical reactions. Here, we present the first biochemical and structural data for a GRE-type diol dehydratase from the organism Roseburia inulinivorans (RiDD). Despite high sequence (48% identity) and structural similarity to the GRE-type glycerol dehydratase from Clostridium butyricum, we demonstrate that the RiDD is in fact a diol dehydratase. In addition, the RiDD will utilize both (S)-1,2- propanediol and (R)-1,2-propanediol as a substrate, with an observed preference for the S enantiomer. Based on the new structural information we developed and successfully tested a hypothesis that explains the functional differences we observe.
- Authors:
-
- Univ. of Georgia, Athens, GA (United States)
- Braskem S.A., São Paulo, SP (Brazil)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1324768
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Biological Chemistry
- Additional Journal Information:
- Journal Volume: 291; Journal Issue: 30; Journal ID: ISSN 0021-9258
- Publisher:
- American Society for Biochemistry and Molecular Biology
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; chemical biology; enzyme structure; intestinal metabolism; metabolic engineering; radical; diol dehydratase; glycerol dehydratase; glycyl radical enzyme; radical dehydration
Citation Formats
LaMattina, Joseph W., Keul, Nicholas D., Reitzer, Pierre, Kapoor, Suraj, Galzerani, Felipe, Koch, Daniel J., Gouvea, Iuri E., and Lanzilotta, William N. 1,2-Propanediol Dehydration in Roseburia inulinivorans: Structural Basis for Substrate and Enantiomer Selectivity. United States: N. p., 2016.
Web. doi:10.1074/jbc.m116.721142.
LaMattina, Joseph W., Keul, Nicholas D., Reitzer, Pierre, Kapoor, Suraj, Galzerani, Felipe, Koch, Daniel J., Gouvea, Iuri E., & Lanzilotta, William N. 1,2-Propanediol Dehydration in Roseburia inulinivorans: Structural Basis for Substrate and Enantiomer Selectivity. United States. https://doi.org/10.1074/jbc.m116.721142
LaMattina, Joseph W., Keul, Nicholas D., Reitzer, Pierre, Kapoor, Suraj, Galzerani, Felipe, Koch, Daniel J., Gouvea, Iuri E., and Lanzilotta, William N. Wed .
"1,2-Propanediol Dehydration in Roseburia inulinivorans: Structural Basis for Substrate and Enantiomer Selectivity". United States. https://doi.org/10.1074/jbc.m116.721142. https://www.osti.gov/servlets/purl/1324768.
@article{osti_1324768,
title = {1,2-Propanediol Dehydration in Roseburia inulinivorans: Structural Basis for Substrate and Enantiomer Selectivity},
author = {LaMattina, Joseph W. and Keul, Nicholas D. and Reitzer, Pierre and Kapoor, Suraj and Galzerani, Felipe and Koch, Daniel J. and Gouvea, Iuri E. and Lanzilotta, William N.},
abstractNote = {Glycyl radical enzymes (GREs) represent a diverse superfamily of enzymes that utilize a radical mechanism to catalyze difficult, but often essential, chemical reactions. Here, we present the first biochemical and structural data for a GRE-type diol dehydratase from the organism Roseburia inulinivorans (RiDD). Despite high sequence (48% identity) and structural similarity to the GRE-type glycerol dehydratase from Clostridium butyricum, we demonstrate that the RiDD is in fact a diol dehydratase. In addition, the RiDD will utilize both (S)-1,2- propanediol and (R)-1,2-propanediol as a substrate, with an observed preference for the S enantiomer. Based on the new structural information we developed and successfully tested a hypothesis that explains the functional differences we observe.},
doi = {10.1074/jbc.m116.721142},
journal = {Journal of Biological Chemistry},
number = 30,
volume = 291,
place = {United States},
year = {Wed Jun 01 00:00:00 EDT 2016},
month = {Wed Jun 01 00:00:00 EDT 2016}
}
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