Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Assessment of photosynthesis regulation in mixotrophically cultured microalga Chlorella sorokiniana

Abstract

Mixotrophic growth of microalgae offers great potential as an efficient strategy for biofuel production. In this study, photosynthetic regulation of mixotrophically cultured Chlorella sorokiniana cells was systematically evaluated. Mixotrophic cells in the exponential growth phase showed the highest photosynthetic activity, where maximum photosynthetic O2 evolution was approximately 3- and 4-fold higher than cells in the same phase grown photoautotrophically in 1% CO2 (in air) and air, respectively. Additionally, characteristic chlorophyll fluorescence parameters demonstrated that no limitation in electron transport downstream of PSII was detected in mixotrophic cells. Up-regulation of photosynthetic activity was associated with high total ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylase activity and expression level of phosphoribulokinase (PRK). After 3 days, photosynthetic O2 evolution of mixotrophic cells that went to the stationary phase, was strongly reduced, with reduced photochemical efficiency and reorganization of the PSII complex. Simultaneously, enzymatic activity for Rubisco carboxylase and mRNA levels of Rubisco and PRK diminished. Importantly, there was almost no non-photochemical quenching for mixotrophic cells, whether grown in log or stationary phase. A decline in the quantum efficiency of PSII and an oxidized plastoquinone pool (PQ pool) was observed under N-depleted conditions during mixotrophic growth. Finally, these results demonstrate that photosynthesis is regulated differently inmore » mixotrophically cultured C. sorokiniana cells than in cells grown under photoautotrophic conditions, with a particularly strong impact by nitrogen levels in the cells.« less

Authors:
 [1];  [1];  [1];  [2];  [1];  [3];  [1];  [1]
+ Show Author Affiliations
  1. Washington State Univ., Pullman, WA (United States)
  2. Johns Hopkins Univ., Baltimore, MD (United States)
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1290785
Alternate Identifier(s):
OSTI ID: 1358745
Report Number(s):
NREL/JA-5100-66919
Journal ID: ISSN 2211-9264
Grant/Contract Number:  
AC36-08GO28308; EE0003112
Resource Type:
Accepted Manuscript
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 19; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; microalgae; Chlorella sorokiniana; mixotrophic; photosynthesis

Citation Formats

Li, Tingting, Kirchhoff, Helmut, Gargouri, Mahmoud, Feng, Jie, Cousins, Asaph B., Pienkos, Philip T., Gang, David R., and Chen, Shulin. Assessment of photosynthesis regulation in mixotrophically cultured microalga Chlorella sorokiniana. United States: N. p., 2016. Web. doi:10.1016/j.algal.2016.07.012.
Copy to clipboard
Li, Tingting, Kirchhoff, Helmut, Gargouri, Mahmoud, Feng, Jie, Cousins, Asaph B., Pienkos, Philip T., Gang, David R., & Chen, Shulin. Assessment of photosynthesis regulation in mixotrophically cultured microalga Chlorella sorokiniana. United States. https://doi.org/10.1016/j.algal.2016.07.012
Copy to clipboard
Li, Tingting, Kirchhoff, Helmut, Gargouri, Mahmoud, Feng, Jie, Cousins, Asaph B., Pienkos, Philip T., Gang, David R., and Chen, Shulin. Tue . "Assessment of photosynthesis regulation in mixotrophically cultured microalga Chlorella sorokiniana". United States. https://doi.org/10.1016/j.algal.2016.07.012. https://www.osti.gov/servlets/purl/1290785.
Copy to clipboard
@article{osti_1290785,
title = {Assessment of photosynthesis regulation in mixotrophically cultured microalga Chlorella sorokiniana},
author = {Li, Tingting and Kirchhoff, Helmut and Gargouri, Mahmoud and Feng, Jie and Cousins, Asaph B. and Pienkos, Philip T. and Gang, David R. and Chen, Shulin},
abstractNote = {Mixotrophic growth of microalgae offers great potential as an efficient strategy for biofuel production. In this study, photosynthetic regulation of mixotrophically cultured Chlorella sorokiniana cells was systematically evaluated. Mixotrophic cells in the exponential growth phase showed the highest photosynthetic activity, where maximum photosynthetic O2 evolution was approximately 3- and 4-fold higher than cells in the same phase grown photoautotrophically in 1% CO2 (in air) and air, respectively. Additionally, characteristic chlorophyll fluorescence parameters demonstrated that no limitation in electron transport downstream of PSII was detected in mixotrophic cells. Up-regulation of photosynthetic activity was associated with high total ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylase activity and expression level of phosphoribulokinase (PRK). After 3 days, photosynthetic O2 evolution of mixotrophic cells that went to the stationary phase, was strongly reduced, with reduced photochemical efficiency and reorganization of the PSII complex. Simultaneously, enzymatic activity for Rubisco carboxylase and mRNA levels of Rubisco and PRK diminished. Importantly, there was almost no non-photochemical quenching for mixotrophic cells, whether grown in log or stationary phase. A decline in the quantum efficiency of PSII and an oxidized plastoquinone pool (PQ pool) was observed under N-depleted conditions during mixotrophic growth. Finally, these results demonstrate that photosynthesis is regulated differently in mixotrophically cultured C. sorokiniana cells than in cells grown under photoautotrophic conditions, with a particularly strong impact by nitrogen levels in the cells.},
doi = {10.1016/j.algal.2016.07.012},
journal = {Algal Research},
number = ,
volume = 19,
place = {United States},
year = {Tue Jul 19 00:00:00 EDT 2016},
month = {Tue Jul 19 00:00:00 EDT 2016}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1016/j.algal.2016.07.012
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 38 works
Citation information provided by
Web of Science
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Mixotrophic cultivation of a Chlorella sorokiniana strain for enhanced biomass and lipid production
journal, July 2014

Mixotrophic growth ofChlorella sorokiniana in outdoor enclosed photobioreactor
journal, March 1996

  • Lee, Yuan-Kun; Ding, Sun-Yeun; Hoe, Chye-Hock
  • Journal of Applied Phycology, Vol. 8, Issue 2
  • DOI: 10.1007/BF02186320

System Response of Metabolic Networks in Chlamydomonas reinhardtii to Total Available Ammonium
journal, July 2012

  • Lee, Do Yup; Park, Jeong-Jin; Barupal, Dinesh K.
  • Molecular & Cellular Proteomics, Vol. 11, Issue 10
  • DOI: 10.1074/mcp.M111.016733

Dynamic flexibility in the light reactions of photosynthesis governed by both electron and proton transfer reactions
journal, July 2004

Effects of Acetate on Facultative Autotrophy in Chlamydomonas reinhardtii Assessed by Photosynthetic Measurements and Stable Isotope Analyses
journal, April 2000

  • Heifetz, Peter B.; Förster, Britta; Osmond, C. Barry
  • Plant Physiology, Vol. 122, Issue 4
  • DOI: 10.1104/pp.122.4.1439

Effects of Light and Glycerol on the Organization of the Photosynthetic Apparatus in the Facultative Heterotroph Pyrenomonas Salina (Cryptophyceae)1
journal, October 1991

Effects of organic carbon sources on growth, photosynthesis, and respiration of Phaeodactylum tricornutum
journal, September 2008

Interactions between organic and inorganic carbon sources during mixotrophic cultivation of Synechococcus sp.
journal, September 2004

The two genes for the small subunit of RuBP Carboxylase/oxygenase are closely linked in Chlamydomonas reinhardtii
journal, January 1986

  • Goldschmidt-Clermont, Michel
  • Plant Molecular Biology, Vol. 6, Issue 1
  • DOI: 10.1007/BF00021302

Regulation of photosynthesis in the unicellular acidophilic red alga Galdieria sulphuraria†: Regulation of photosynthesis in Galdieria
journal, June 2007

Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid
journal, January 1975

  • Cataldo, D. A.; Maroon, M.; Schrader, L. E.
  • Communications in Soil Science and Plant Analysis, Vol. 6, Issue 1
  • DOI: 10.1080/00103627509366547

Chlorophyll Fluorescence: A Probe of Photosynthesis In Vivo
journal, June 2008

Structural and functional self-organization of Photosystem II in grana thylakoids
journal, September 2007

  • Kirchhoff, Helmut; Haase, Winfried; Haferkamp, Silvia
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1767, Issue 9
  • DOI: 10.1016/j.bbabio.2007.05.009

Temperature response of in vivo  Rubisco kinetics and mesophyll conductance in Arabidopsis thaliana : comparisons to Nicotiana tabacum
journal, August 2013

  • Walker, Berkley; Ariza, Loren S.; Kaines, Sarah
  • Plant, Cell & Environment, Vol. 36, Issue 12
  • DOI: 10.1111/pce.12166

Regulation of starch and lipid accumulation in a microalga Chlorella sorokiniana
journal, March 2015

Non-Photochemical Quenching. A Response to Excess Light Energy
journal, April 2001

  • Müller, Patricia; Li, Xiao-Ping; Niyogi, Krishna K.
  • Plant Physiology, Vol. 125, Issue 4
  • DOI: 10.1104/pp.125.4.1558

An ancient light-harvesting protein is critical for the regulation of algal photosynthesis
journal, November 2009

  • Peers, Graham; Truong, Thuy B.; Ostendorf, Elisabeth
  • Nature, Vol. 462, Issue 7272
  • DOI: 10.1038/nature08587

Differential thermal effects on the energy distribution between photosystem II and photosystem I in thylakoid membranes of a psychrophilic and a mesophilic alga
journal, April 2002

  • Morgan-Kiss, Rachael; Ivanov, Alexander G.; Williams, John
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1561, Issue 2
  • DOI: 10.1016/S0005-2736(02)00352-8

Efficient Light Harvesting by Photosystem II Requires an Optimized Protein Packing Density in Grana Thylakoids
journal, April 2010

  • Haferkamp, Silvia; Haase, Winfried; Pascal, Andrew A.
  • Journal of Biological Chemistry, Vol. 285, Issue 22
  • DOI: 10.1074/jbc.M109.077750

Coordination of photosynthetic and respiratory metabolism in Chlorella vulgaris UAM 101 in the light
journal, August 1995

Uptake and Utilization of Fructose by Anabaena variabilis ATCC 29413. Effect on Respiration and Photosynthesis
journal, April 1992

Regulation of Periplasmic Carbonic Anhydrase Expression in Chlamydomonas reinhardtii by Acetate and pH
journal, September 1994

Thylakoid Membrane Reduction Affects the Photosystem Stoichiometry in the Cyanobacterium Synechocystis sp. PCC 6803
journal, December 2008

  • Fuhrmann, Eva; Gathmann, Sven; Rupprecht, Eva
  • Plant Physiology, Vol. 149, Issue 2
  • DOI: 10.1104/pp.108.132373

ATP control on state transitions in vivo in Chlamydomonas reinhardtii
journal, October 1990

  • Bulté, Laurence; Gans, Pierre; Rebéillé, Fabrice
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1020, Issue 1
  • DOI: 10.1016/0005-2728(90)90095-L

Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials
journal, May 2011

Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions
journal, March 2009

Effects of Inorganic n Availability on Algal Photosynthesis and Carbon Metabolism
journal, February 1991

Interactions Among Irradiance, Oxygen Evolution and Nitrite Uptake by Chlamydomonas (Chlorophyceae)1,2
journal, December 1987

Carbon metabolite feedback regulation of leaf photosynthesis and development
journal, January 2003

  • Paul, M. J.; Pellny, T. K.
  • Journal of Experimental Botany, Vol. 54, Issue 382
  • DOI: 10.1093/jxb/erg052

Arabidopsis Seedling Growth, Storage Lipid Mobilization, and Photosynthetic Gene Expression Are Regulated by Carbon:Nitrogen Availability
journal, February 2002

  • Martin, Thomas; Oswald, Oliver; Graham, Ian A.
  • Plant Physiology, Vol. 128, Issue 2
  • DOI: 10.1104/pp.010475

Acclimation of photosynthesis to elevated CO2 through feedback regulation of gene expression: Climate of opinion
journal, June 1996

  • Van Oosten, Jean-Jacques; Besford, Robert T.
  • Photosynthesis Research, Vol. 48, Issue 3
  • DOI: 10.1007/BF00029468

Increased capacity for photosynthesis in wheat grown at elevated CO2: the relationship between electron transport and carbon metabolism
journal, October 1995

  • Habash, DimahZ.; Paul, MatthewJ.; Parry, MartinA. J.
  • Planta, Vol. 197, Issue 3
  • DOI: 10.1007/BF00196670
    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    Molecular basis of autotrophic vs mixotrophic growth in Chlorella sorokiniana
    journal, April 2018

    Cell growth and lipid accumulation of a microalgal mutant Scenedesmus sp. Z-4 by combining light/dark cycle with temperature variation
    journal, November 2017

    Glucose addition-induced changes in the growth and chemical compositions of a freshwater microalga Chlorella kessleri: Impacts of glucose addition in Chlorella kessleri
    journal, December 2018

    • Deng, Xiang-Yuan; Xue, Chun-Ye; Chen, Biao
    • Journal of Chemical Technology & Biotechnology, Vol. 94, Issue 4
    • DOI: 10.1002/jctb.5870

    Respirometry as a tool to quantify kinetic parameters of microalgal mixotrophic growth
    journal, February 2019

    • Sforza, Eleonora; Pastore, Martina; Barbera, Elena
    • Bioprocess and Biosystems Engineering, Vol. 42, Issue 5
    • DOI: 10.1007/s00449-019-02087-9
      Sort by:
      [ × clear filter / sort ]

      Similar Records in DOE PAGES and OSTI.GOV collections: