IV Administered Gadodiamide Enters the Lumen of the Prostatic Glands: X-Ray Fluorescence Microscopy Examination of a Mouse Model

Mustafi, Devkumar; Gleber, Sophie-Charlotte; Ward, Jesse; Dougherty, Urszula; Zamora, Marta; Markiewicz, Erica; Binder, David C.; Antic, Tatjana; Vogt, Stefan; Karczmar, Gregory S.; Oto, Aytekin

doi:10.2214/ajr.14.14055

Title: IV Administered Gadodiamide Enters the Lumen of the Prostatic Glands: X-Ray Fluorescence Microscopy Examination of a Mouse Model

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Abstract

In our objective, we descibe how dynamic contrast-enhanced MRI (DCE-MRI) has become a standard component of multiparametric protocols for MRI examination of the prostate, and its use is incorporated into current guidelines for prostate MRI examination. Analysis of DCE-MRI data for the prostate is usually based on the distribution of gadolinium-based agents, such as gadodiamide, into two well-mixed compartments, and it assumes that gadodiamide does not enter into the glandular lumen. However, this assumption has not been directly tested. The purpose of this study was to use x-ray fluorescence microscopy (XFM) imaging in situ to measure the concentration of gadodiamide in the epithelia and lumens of the prostate of healthy mice after IV injection of the contrast agent. For our materials and methods, six C57Bl6 male mice (age, 28 weeks) were sacrificed 10 minutes after IV injection of gadodiamide (0.13 mmol/kg), and three mice were sacrificed after saline injection. Prostate tissue samples obtained from each mouse were harvested and frozen; 7-μm-thick slices were sectioned for XFM imaging, and adjacent 5-μm-thick slices were sectioned for H and E staining. Elemental concentrations were determined from XFM images. Our results show mean (± SD) baseline concentration of gadolinium of 0.01 ± 0.01 mMmore »« less

Authors:

Mustafi, Devkumar ^[1]; Gleber, Sophie-Charlotte ^[2]; Ward, Jesse ^[2]; Dougherty, Urszula ^[3]; Zamora, Marta ^[1]; Markiewicz, Erica ^[1]; Binder, David C. ^[1]; Antic, Tatjana ^[4]; Vogt, Stefan ^[2]; Karczmar, Gregory S. ^[1]; Oto, Aytekin ^[1]

Univ. of Chicago, IL (United States). Dept of Radiology
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Univ. of Chicago, IL (United States). Dept. of Medicine
Univ. of Chicago, IL (United States). Dept of Pathology

Publication Date:: Tue Sep 01 00:00:00 EDT 2015

Research Org.:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Org.:: USDOE Office of Science (SC); National Institutes of Health (NIH)

OSTI Identifier:: 1280923

Grant/Contract Number:: AC02-06CH11357; RO1-CA133490; RO1-172801

Resource Type:: Accepted Manuscript

Journal Name:: AJR, American Journal of Roentgenology

Additional Journal Information:: Journal Volume: 205; Journal Issue: 3; Journal ID: ISSN 0361-803X

Publisher:: American Roentgen Ray Society (ARRS)

Country of Publication:: United States

Language:: English

Subject:: 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; dynamic contrast-enhanced MRI; gadodiamide distribution in prostatic tissues; mouse prostate; prostatic lumen; x-ray fluorescence microscopy

Citation Formats


                    Mustafi, Devkumar, Gleber, Sophie-Charlotte, Ward, Jesse, Dougherty, Urszula, Zamora, Marta, Markiewicz, Erica, Binder, David C., Antic, Tatjana, Vogt, Stefan, Karczmar, Gregory S., and Oto, Aytekin. IV Administered Gadodiamide Enters the Lumen of the Prostatic Glands: X-Ray Fluorescence Microscopy Examination of a Mouse Model.  United States: N. p., 2015. 
Web.  doi:10.2214/ajr.14.14055.

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                    Mustafi, Devkumar, Gleber, Sophie-Charlotte, Ward, Jesse, Dougherty, Urszula, Zamora, Marta, Markiewicz, Erica, Binder, David C., Antic, Tatjana, Vogt, Stefan, Karczmar, Gregory S., & Oto, Aytekin. IV Administered Gadodiamide Enters the Lumen of the Prostatic Glands: X-Ray Fluorescence Microscopy Examination of a Mouse Model.  United States.  https://doi.org/10.2214/ajr.14.14055

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                    Mustafi, Devkumar, Gleber, Sophie-Charlotte, Ward, Jesse, Dougherty, Urszula, Zamora, Marta, Markiewicz, Erica, Binder, David C., Antic, Tatjana, Vogt, Stefan, Karczmar, Gregory S., and Oto, Aytekin. Tue .  
"IV Administered Gadodiamide Enters the Lumen of the Prostatic Glands: X-Ray Fluorescence Microscopy Examination of a Mouse Model".  United States.  https://doi.org/10.2214/ajr.14.14055.  https://www.osti.gov/servlets/purl/1280923.

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@article{osti_1280923,

  title        = {IV Administered Gadodiamide Enters the Lumen of the Prostatic Glands: X-Ray Fluorescence Microscopy Examination of a Mouse Model},

  author       = {Mustafi, Devkumar and Gleber, Sophie-Charlotte and Ward, Jesse and Dougherty, Urszula and Zamora, Marta and Markiewicz, Erica and Binder, David C. and Antic, Tatjana and Vogt, Stefan and Karczmar, Gregory S. and Oto, Aytekin},

  abstractNote = {In our objective, we descibe how dynamic contrast-enhanced MRI (DCE-MRI) has become a standard component of multiparametric protocols for MRI examination of the prostate, and its use is incorporated into current guidelines for prostate MRI examination. Analysis of DCE-MRI data for the prostate is usually based on the distribution of gadolinium-based agents, such as gadodiamide, into two well-mixed compartments, and it assumes that gadodiamide does not enter into the glandular lumen. However, this assumption has not been directly tested. The purpose of this study was to use x-ray fluorescence microscopy (XFM) imaging in situ to measure the concentration of gadodiamide in the epithelia and lumens of the prostate of healthy mice after IV injection of the contrast agent. For our materials and methods, six C57Bl6 male mice (age, 28 weeks) were sacrificed 10 minutes after IV injection of gadodiamide (0.13 mmol/kg), and three mice were sacrificed after saline injection. Prostate tissue samples obtained from each mouse were harvested and frozen; 7-μm-thick slices were sectioned for XFM imaging, and adjacent 5-μm-thick slices were sectioned for H and E staining. Elemental concentrations were determined from XFM images. Our results show mean (± SD) baseline concentration of gadolinium of 0.01 ± 0.01 mM was determined from XFM measurements of prostatic tissue samples when no gadodiamide was administered, and it was used to determine the measurement error. When gadodiamide was added, the mean concentrations of gadolinium in the epithelia and lumens in 32 prostatic glands from six mice were 1.00 ± 0.13 and 0.36 ± 0.09 mM, respectively. In conclusion, our data suggest that IV administration of gadodiamide results in uptake of contrast agent by the glandular lumens of the mouse prostate. We were able to quantitatively determine gadodiamide distributions in mouse prostatic epithelia and lumens.},

  doi          = {10.2214/ajr.14.14055},

  journal      = {AJR, American Journal of Roentgenology},

  number       = 3,

  volume       = 205,

  place        = {United States},

  year         = {Tue Sep 01 00:00:00 EDT 2015},

  month        = {Tue Sep 01 00:00:00 EDT 2015}

}

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