Recent Advances in Algal Genetic Tool Development
Abstract
The goal of achieving cost-effective biofuels and bioproducts derived from algal biomass will require improvements along the entire value chain, including identification of robust, high-productivity strains and development of advanced genetic tools. Though there have been modest advances in development of genetic systems for the model alga Chlamydomonas reinhardtii, progress in development of algal genetic tools, especially as applied to non-model algae, has generally lagged behind that of more commonly utilized laboratory and industrial microbes. This is in part due to the complex organellar structure of algae, including robust cell walls and intricate compartmentalization of target loci, as well as prevalent gene silencing mechanisms, which hinder facile utilization of conventional genetic engineering tools and methodologies. However, recent progress in global tool development has opened the door for implementation of strain-engineering strategies in industrially-relevant algal strains. Here, we review recent advances in algal genetic tool development and applications in eukaryotic microalgae.
- Authors:
-
- Colorado School of Mines, Golden, CO (United States). Dept. of Chemistry and Geochemistry
- National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
- Publication Date:
- Research Org.:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
- OSTI Identifier:
- 1271943
- Report Number(s):
- NREL/JA-5100-65870
Journal ID: ISSN 2211-5501
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Current Biotechnology
- Additional Journal Information:
- Journal Volume: 5; Journal Issue: 3; Journal ID: ISSN 2211-5501
- Publisher:
- Bentham Science Publishers
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; algae; genetic engineering; biofuels; bioproducts; synthetic biology; photosynthesis
Citation Formats
R. Dahlin, Lukas, and T. Guarnieri, Michael. Recent Advances in Algal Genetic Tool Development. United States: N. p., 2016.
Web. doi:10.2174/2211550105666160127230814.
R. Dahlin, Lukas, & T. Guarnieri, Michael. Recent Advances in Algal Genetic Tool Development. United States. https://doi.org/10.2174/2211550105666160127230814
R. Dahlin, Lukas, and T. Guarnieri, Michael. Fri .
"Recent Advances in Algal Genetic Tool Development". United States. https://doi.org/10.2174/2211550105666160127230814. https://www.osti.gov/servlets/purl/1271943.
@article{osti_1271943,
title = {Recent Advances in Algal Genetic Tool Development},
author = {R. Dahlin, Lukas and T. Guarnieri, Michael},
abstractNote = {The goal of achieving cost-effective biofuels and bioproducts derived from algal biomass will require improvements along the entire value chain, including identification of robust, high-productivity strains and development of advanced genetic tools. Though there have been modest advances in development of genetic systems for the model alga Chlamydomonas reinhardtii, progress in development of algal genetic tools, especially as applied to non-model algae, has generally lagged behind that of more commonly utilized laboratory and industrial microbes. This is in part due to the complex organellar structure of algae, including robust cell walls and intricate compartmentalization of target loci, as well as prevalent gene silencing mechanisms, which hinder facile utilization of conventional genetic engineering tools and methodologies. However, recent progress in global tool development has opened the door for implementation of strain-engineering strategies in industrially-relevant algal strains. Here, we review recent advances in algal genetic tool development and applications in eukaryotic microalgae.},
doi = {10.2174/2211550105666160127230814},
journal = {Current Biotechnology},
number = 3,
volume = 5,
place = {United States},
year = {Fri Jun 24 00:00:00 EDT 2016},
month = {Fri Jun 24 00:00:00 EDT 2016}
}