Transfer of a Catabolic Pathway for Chloromethane in Methylobacterium Strains Highlights Different Limitations for Growth with Chloromethane or with Dichloromethane
Abstract
Chloromethane is an ozone-depleting gas, produced predominantly from natural sources, that provides an important environmental niche for microbes capable of consuming it. Chloromethane catabolism has been difficult to study owing to the challenging genetics of its native microbial hosts. Since the pathways for chloromethane catabolism show evidence of horizontal gene transfer, we reproduced this transfer process in the laboratory to generate new chloromethane-catabolizing strains in tractable hosts. Here, we demonstrate that six putative accessory genes improve chloromethane catabolism, though heterologous expression of only one of the six is strictly necessary for growth on chloromethane. In contrast to growth of Methylobacterium strains with the closely-related compound dichloromethane, we find that chloride export does not limit growth on chloromethane and, in general, that the ability of a strain to grow on dichloromethane is uncorrelated with its ability to grow on chloromethane. Finally, this heterologous expression system allows us to investigate the components required for effective chloromethane catabolism and the factors that limit effective catabolism after horizontal transfer.
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Harvard Univ., Cambridge, MA (United States)
- Univ. of Strasbourg (France)
- Harvard Univ., Cambridge, MA (United States); Univ. of Idaho, Moscow, ID (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1265893
- Grant/Contract Number:
- AC05-00OR22725; F32 GM106629
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Michener, Joshua K., Vuilleumier, Stéphane, Bringel, Françoise, and Marx, Christopher J. Transfer of a Catabolic Pathway for Chloromethane in Methylobacterium Strains Highlights Different Limitations for Growth with Chloromethane or with Dichloromethane. United States: N. p., 2016.
Web. doi:10.3389/fmicb.2016.01116.
Michener, Joshua K., Vuilleumier, Stéphane, Bringel, Françoise, & Marx, Christopher J. Transfer of a Catabolic Pathway for Chloromethane in Methylobacterium Strains Highlights Different Limitations for Growth with Chloromethane or with Dichloromethane. United States. https://doi.org/10.3389/fmicb.2016.01116
Michener, Joshua K., Vuilleumier, Stéphane, Bringel, Françoise, and Marx, Christopher J. Tue .
"Transfer of a Catabolic Pathway for Chloromethane in Methylobacterium Strains Highlights Different Limitations for Growth with Chloromethane or with Dichloromethane". United States. https://doi.org/10.3389/fmicb.2016.01116. https://www.osti.gov/servlets/purl/1265893.
@article{osti_1265893,
title = {Transfer of a Catabolic Pathway for Chloromethane in Methylobacterium Strains Highlights Different Limitations for Growth with Chloromethane or with Dichloromethane},
author = {Michener, Joshua K. and Vuilleumier, Stéphane and Bringel, Françoise and Marx, Christopher J.},
abstractNote = {Chloromethane is an ozone-depleting gas, produced predominantly from natural sources, that provides an important environmental niche for microbes capable of consuming it. Chloromethane catabolism has been difficult to study owing to the challenging genetics of its native microbial hosts. Since the pathways for chloromethane catabolism show evidence of horizontal gene transfer, we reproduced this transfer process in the laboratory to generate new chloromethane-catabolizing strains in tractable hosts. Here, we demonstrate that six putative accessory genes improve chloromethane catabolism, though heterologous expression of only one of the six is strictly necessary for growth on chloromethane. In contrast to growth of Methylobacterium strains with the closely-related compound dichloromethane, we find that chloride export does not limit growth on chloromethane and, in general, that the ability of a strain to grow on dichloromethane is uncorrelated with its ability to grow on chloromethane. Finally, this heterologous expression system allows us to investigate the components required for effective chloromethane catabolism and the factors that limit effective catabolism after horizontal transfer.},
doi = {10.3389/fmicb.2016.01116},
journal = {Frontiers in Microbiology},
number = ,
volume = 7,
place = {United States},
year = {Tue Jul 19 00:00:00 EDT 2016},
month = {Tue Jul 19 00:00:00 EDT 2016}
}
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Methanol consumption drives the bacterial chloromethane sink in a forest soil
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- Chaignaud, Pauline; Morawe, Mareen; Besaury, Ludovic
- The ISME Journal, Vol. 12, Issue 11
Diversity and composition of cave methanotrophic communities
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Microbial phenotypic heterogeneity in response to a metabolic toxin: Continuous, dynamically shifting distribution of formaldehyde tolerance in Methylobacterium extorquens populations
journal, November 2019
- Lee, Jessica A.; Riazi, Siavash; Nemati, Shahla
- PLOS Genetics, Vol. 15, Issue 11
Microbial phenotypic heterogeneity in response to a metabolic toxin: continuous, dynamically shifting distribution of formaldehyde tolerance in Methylobacterium extorquens populations
journal, September 2019
- Lee, Jessica A.; Riazi, Siavash; Nemati, Shahla H.
- PLOS Genetics
Microbial phenotypic heterogeneity in response to a metabolic toxin: Continuous, dynamically shifting distribution of formaldehyde tolerance in Methylobacterium extorquens populations
journal, November 2019
- Lee, Jessica A.; Riazi, Siavash; Nemati, Shahla
- PLOS Genetics, Vol. 15, Issue 11