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Title: Microstencils to generate defined, multi-species patterns of bacteria

Abstract

Microbial communities are complex heterogeneous systems that are influenced by physical and chemical interactions with their environment, host, and community members. Techniques that facilitate the quantitative evaluation of how microscale organization influences the morphogenesis of multispecies communities could provide valuable insights into the dynamic behavior and organization of natural communities, the design of synthetic environments for multispecies culture, and the engineering of artificial consortia. In this work, we demonstrate a method for patterning microbes into simple arrangements that allow the quantitative measurement of growth dynamics as a function of their proximity to one another. The method combines parylene-based liftoff techniques with microfluidic delivery to simultaneously pattern multiple bacterial species with high viability using low-cost, customizable methods. Furthermore, quantitative measurements of bacterial growth for two competing isolates demonstrate that spatial coordination can play a critical role in multispecies growth and structure.

Authors:
 [1];  [1];  [2];  [2];  [3]
+ Show Author Affiliations
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Kansas State Univ., Manhattan, KS (United States)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1263870
Alternate Identifier(s):
OSTI ID: 1234320
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Biomicrofluidics
Additional Journal Information:
Journal Volume: 9; Journal Issue: 6; Journal ID: ISSN 1932-1058
Publisher:
American Institute of Physics (AIP)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; surface patterns; bacteria; cell growth; microfluidics; silicon

Citation Formats

Doktycz, Mitchel J., Retterer, Scott T., Pelletier, Dale A., Timm, Collin M., and Hansen, Ryan R. Microstencils to generate defined, multi-species patterns of bacteria. United States: N. p., 2015. Web. doi:10.1063/1.4935938.
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Doktycz, Mitchel J., Retterer, Scott T., Pelletier, Dale A., Timm, Collin M., & Hansen, Ryan R. Microstencils to generate defined, multi-species patterns of bacteria. United States. https://doi.org/10.1063/1.4935938
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Doktycz, Mitchel J., Retterer, Scott T., Pelletier, Dale A., Timm, Collin M., and Hansen, Ryan R. Thu . "Microstencils to generate defined, multi-species patterns of bacteria". United States. https://doi.org/10.1063/1.4935938. https://www.osti.gov/servlets/purl/1263870.
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@article{osti_1263870,
title = {Microstencils to generate defined, multi-species patterns of bacteria},
author = {Doktycz, Mitchel J. and Retterer, Scott T. and Pelletier, Dale A. and Timm, Collin M. and Hansen, Ryan R.},
abstractNote = {Microbial communities are complex heterogeneous systems that are influenced by physical and chemical interactions with their environment, host, and community members. Techniques that facilitate the quantitative evaluation of how microscale organization influences the morphogenesis of multispecies communities could provide valuable insights into the dynamic behavior and organization of natural communities, the design of synthetic environments for multispecies culture, and the engineering of artificial consortia. In this work, we demonstrate a method for patterning microbes into simple arrangements that allow the quantitative measurement of growth dynamics as a function of their proximity to one another. The method combines parylene-based liftoff techniques with microfluidic delivery to simultaneously pattern multiple bacterial species with high viability using low-cost, customizable methods. Furthermore, quantitative measurements of bacterial growth for two competing isolates demonstrate that spatial coordination can play a critical role in multispecies growth and structure.},
doi = {10.1063/1.4935938},
journal = {Biomicrofluidics},
number = 6,
volume = 9,
place = {United States},
year = {Thu Nov 12 00:00:00 EST 2015},
month = {Thu Nov 12 00:00:00 EST 2015}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1063/1.4935938
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