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Title: HemQ: An iron-coproporphyrin oxidative decarboxylase for protoheme synthesis in Firmicutes and Actinobacteria

Abstract

Genes for chlorite dismutase-like proteins are found widely among heme-synthesizing bacteria and some Archaea. It is now known that among the Firmicutes and Actinobacteria these proteins do not possess chlorite dismutase activity but instead are essential for heme synthesis. These proteins, named HemQ, are ironcoproporphyrin (coproheme) decarboxylases that catalyze the oxidative decarboxylation of coproheme III into protoheme IX. As purified, HemQs do not contain bound heme, but readily bind exogeneously supplied heme with low micromolar affinity. We find that the heme-bound form of HemQ has low peroxidase activity and in the presence of peroxide the bound heme may be destroyed. Furthermore, it is possible that HemQ may serve a dual role as a decarboxylase in heme biosynthesis and a regulatory protein in heme homeostasis.

Authors:
 [1];  [2]
+ Show Author Affiliations
  1. Univ. of Georgia, Athens, GA (United States). Inst. of Biomedical and Health Sciences
  2. Argonne National Lab. (ANL), Argonne, IL (United States). Mathematics and Computer Science Division
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE; National Institutes of Health (NIH)
OSTI Identifier:
1263632
Grant/Contract Number:  
DK096051
Resource Type:
Accepted Manuscript
Journal Name:
Archives of Biochemistry and Biophysics
Additional Journal Information:
Journal Volume: 574; Journal Issue: C; Journal ID: ISSN 0003-9861
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HemQ; Heme synthesis; coproheme decarboxylase; chlorite dismutase-like protein

Citation Formats

Dailey, Harry A., and Gerdes, Svetlana. HemQ: An iron-coproporphyrin oxidative decarboxylase for protoheme synthesis in Firmicutes and Actinobacteria. United States: N. p., 2015. Web. doi:10.1016/j.abb.2015.02.017.
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Dailey, Harry A., & Gerdes, Svetlana. HemQ: An iron-coproporphyrin oxidative decarboxylase for protoheme synthesis in Firmicutes and Actinobacteria. United States. https://doi.org/10.1016/j.abb.2015.02.017
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Dailey, Harry A., and Gerdes, Svetlana. Sat . "HemQ: An iron-coproporphyrin oxidative decarboxylase for protoheme synthesis in Firmicutes and Actinobacteria". United States. https://doi.org/10.1016/j.abb.2015.02.017. https://www.osti.gov/servlets/purl/1263632.
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@article{osti_1263632,
title = {HemQ: An iron-coproporphyrin oxidative decarboxylase for protoheme synthesis in Firmicutes and Actinobacteria},
author = {Dailey, Harry A. and Gerdes, Svetlana},
abstractNote = {Genes for chlorite dismutase-like proteins are found widely among heme-synthesizing bacteria and some Archaea. It is now known that among the Firmicutes and Actinobacteria these proteins do not possess chlorite dismutase activity but instead are essential for heme synthesis. These proteins, named HemQ, are ironcoproporphyrin (coproheme) decarboxylases that catalyze the oxidative decarboxylation of coproheme III into protoheme IX. As purified, HemQs do not contain bound heme, but readily bind exogeneously supplied heme with low micromolar affinity. We find that the heme-bound form of HemQ has low peroxidase activity and in the presence of peroxide the bound heme may be destroyed. Furthermore, it is possible that HemQ may serve a dual role as a decarboxylase in heme biosynthesis and a regulatory protein in heme homeostasis.},
doi = {10.1016/j.abb.2015.02.017},
journal = {Archives of Biochemistry and Biophysics},
number = C,
volume = 574,
place = {United States},
year = {Sat Feb 21 00:00:00 EST 2015},
month = {Sat Feb 21 00:00:00 EST 2015}
}
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https://doi.org/10.1016/j.abb.2015.02.017
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Works referenced in this record:

Control of 5-aminolaevulinate synthetase activity in Rhodopseudomonas spheroides a role for trisulphides
journal, August 1975

  • Sandy, J. D.; Davies, R. C.; Neuberger, A.
  • Biochemical Journal, Vol. 150, Issue 2
  • DOI: 10.1042/bj1500245

Oxygen-dependent Coproporphyrinogen III Oxidase (HemF) from Escherichia coli Is Stimulated by Manganese
journal, November 2003

  • Breckau, Daniela; Mahlitz, Esther; Sauerwald, Anselm
  • Journal of Biological Chemistry, Vol. 278, Issue 47
  • DOI: 10.1074/jbc.M308553200

Recent advances in the biosynthesis of modified tetrapyrroles: the discovery of an alternative pathway for the formation of heme and heme d 1
journal, February 2014

  • Bali, Shilpa; Palmer, David J.; Schroeder, Susanne
  • Cellular and Molecular Life Sciences, Vol. 71, Issue 15
  • DOI: 10.1007/s00018-014-1563-x

Evolution of enzymes and pathways for the biosynthesis of cofactors
journal, January 2007

  • Holliday, Gemma L.; Thornton, Janet M.; Marquet, Andrée
  • Natural Product Reports, Vol. 24, Issue 5
  • DOI: 10.1039/b703107f

The Silencing of Pseudogenes
journal, July 2005

  • Mira, Alex; Pushker, Ravindra
  • Molecular Biology and Evolution, Vol. 22, Issue 11
  • DOI: 10.1093/molbev/msi209

tRNAGlu as a cofactor in δ-aminolevulinate biosynthesis: steps that regulate chlorophyll synthesis
journal, April 1988

  • Gamini Kannangara, C.; Gough, Simon P.; Bruyant, Philippe
  • Trends in Biochemical Sciences, Vol. 13, Issue 4
  • DOI: 10.1016/0968-0004(88)90071-0

Structure of porphobilinogen deaminase reveals a flexible multidomain polymerase with a single catalytic site
journal, September 1992

  • Louie, Gordon V.; Brownlie, Paul D.; Lambert, Richard
  • Nature, Vol. 359, Issue 6390
  • DOI: 10.1038/359033a0

A π-Helix Switch Selective for Porphyrin Deprotonation and Product Release in Human Ferrochelatase
journal, November 2007

  • Medlock, Amy E.; Dailey, Tamara A.; Ross, Teresa A.
  • Journal of Molecular Biology, Vol. 373, Issue 4
  • DOI: 10.1016/j.jmb.2007.08.040

The Alternative Route to Heme in the Methanogenic Archaeon Methanosarcina barkeri
journal, January 2014

  • Kühner, Melanie; Haufschildt, Kristin; Neumann, Alexander
  • Archaea, Vol. 2014
  • DOI: 10.1155/2014/327637

Radical S-Adenosylmethionine Enzyme Coproporphyrinogen III Oxidase HemN
journal, August 2005

  • Layer, Gunhild; Grage, Katrin; Teschner, Thomas
  • Journal of Biological Chemistry, Vol. 280, Issue 32
  • DOI: 10.1074/jbc.M501275200

Heme biosynthesis is coupled to electron transport chains for energy generation
journal, May 2010

  • Mobius, K.; Arias-Cartin, R.; Breckau, D.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 23
  • DOI: 10.1073/pnas.1000956107

Molecular hijacking of siroheme for the synthesis of heme and d1 heme
journal, October 2011

  • Bali, S.; Lawrence, A. D.; Lobo, S. A.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 45
  • DOI: 10.1073/pnas.1108228108

Characterization of the Late Steps of Microbial Heme Synthesis: Conversion of Coproporphyrinogen to Protoporphyrin
journal, July 1971

Product Release Rather than Chelation Determines Metal Specificity for Ferrochelatase
journal, October 2009

  • Medlock, Amy E.; Carter, Michael; Dailey, Tamara A.
  • Journal of Molecular Biology, Vol. 393, Issue 2
  • DOI: 10.1016/j.jmb.2009.08.042

Glutamyl-transfer RNA: a precursor of heme and chlorophyll biosynthesis
journal, June 1992

Uroporphyrinogen decarboxylation as a benchmark for the catalytic proficiency of enzymes
journal, November 2008

  • Lewis, C. A.; Wolfenden, R.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 45
  • DOI: 10.1073/pnas.0809838105

Evolutionary Relationship between Initial Enzymes of Tetrapyrrole Biosynthesis
journal, May 2006

  • Schulze, Jörg O.; Schubert, Wolf-Dieter; Moser, Jürgen
  • Journal of Molecular Biology, Vol. 358, Issue 5
  • DOI: 10.1016/j.jmb.2006.02.064

Discovery and Characterization of HemQ
journal, August 2010

  • Dailey, Tamara A.; Boynton, Tye O.; Albetel, Angela-Nadia
  • Journal of Biological Chemistry, Vol. 285, Issue 34
  • DOI: 10.1074/jbc.M110.142604

Crystal Structure of Uroporphyrinogen Decarboxylase from Bacillus subtilis
journal, May 2007

  • Fan, Jun; Liu, Qun; Hao, Quan
  • Journal of Bacteriology, Vol. 189, Issue 9
  • DOI: 10.1128/JB.01083-06

Evolutionary relationships among pyridoxal-5'-phosphate-dependent enzymes. Regio-specific alpha, beta and gamma families
journal, February 1994

Identification of Escherichia coli HemG as a Novel, Menadione-Dependent Flavodoxin with Protoporphyrinogen Oxidase Activity
journal, July 2009

  • Boynton, Tye O.; Daugherty, Lauren E.; Dailey, Tamara A.
  • Biochemistry, Vol. 48, Issue 29
  • DOI: 10.1021/bi900850y

The Chlorite Dismutase (HemQ) from Staphylococcus aureus Has a Redox-sensitive Heme and Is Associated with the Small Colony Variant Phenotype
journal, August 2013

  • Mayfield, Jeffrey A.; Hammer, Neal D.; Kurker, Richard C.
  • Journal of Biological Chemistry, Vol. 288, Issue 32
  • DOI: 10.1074/jbc.M112.442335

Protoporphyrinogen Oxidase of Myxococcus xanthus
journal, April 1996

  • Dailey, Harry A.; Dailey, Tamara A.
  • Journal of Biological Chemistry, Vol. 271, Issue 15
  • DOI: 10.1074/jbc.271.15.8714

Molecular diversity and ecology of microbial plankton
journal, September 2005

Structure and function of enzymes in heme biosynthesis
journal, May 2010

  • Layer, Gunhild; Reichelt, Joachim; Jahn, Dieter
  • Protein Science, Vol. 19, Issue 6
  • DOI: 10.1002/pro.405

SAR11 clade dominates ocean surface bacterioplankton communities
journal, December 2002

  • Morris, Robert M.; Rappé, Michael S.; Connon, Stephanie A.
  • Nature, Vol. 420, Issue 6917
  • DOI: 10.1038/nature01240

Understanding How the Distal Environment Directs Reactivity in Chlorite Dismutase: Spectroscopy and Reactivity of Arg183 Mutants
journal, February 2012

  • Blanc, Béatrice; Mayfield, Jeffery A.; McDonald, Claudia A.
  • Biochemistry, Vol. 51, Issue 9
  • DOI: 10.1021/bi2017377

Discovery of a Gene Involved in a Third Bacterial Protoporphyrinogen Oxidase Activity through Comparative Genomic Analysis and Functional Complementation
journal, June 2011

  • Boynton, Tye O.; Gerdes, Svetlana; Craven, Sarah H.
  • Applied and Environmental Microbiology, Vol. 77, Issue 14
  • DOI: 10.1128/AEM.00171-11

Manipulating Conserved Heme Cavity Residues of Chlorite Dismutase: Effect on Structure, Redox Chemistry, and Reactivity
journal, December 2013

  • Hofbauer, Stefan; Gysel, Kira; Bellei, Marzia
  • Biochemistry, Vol. 53, Issue 1
  • DOI: 10.1021/bi401042z

Crystal structure of 5-aminolevulinate synthase, the first enzyme of heme biosynthesis, and its link to XLSA in humans
journal, August 2005

  • Astner, Isabel; Schulze, Jörg O.; van den Heuvel, Joop
  • The EMBO Journal, Vol. 24, Issue 18
  • DOI: 10.1038/sj.emboj.7600792

Altered Orientation of Active Site Residues in Variants of Human Ferrochelatase. Evidence for a Hydrogen Bond Network Involved in Catalysis
journal, June 2007

  • Dailey, Harry A.; Wu, Chia-Kuei; Horanyi, Peter
  • Biochemistry, Vol. 46, Issue 27
  • DOI: 10.1021/bi700151f

X-ray structure of 5-aminolaevulinate dehydratase, a hybrid aldolase
journal, December 1997

  • Erskine, Peter T.; Senior, Natalie; Awan, Sarah
  • Nature Structural Biology, Vol. 4, Issue 12
  • DOI: 10.1038/nsb1297-1025

HutZ Is Required for Efficient Heme Utilization in Vibrio cholerae
journal, July 2004

The Synthesis of Enzymes Concerned in Bacterio-chlorophyll Formation in Growing Cultures of Rhodopseudomonas spheroides
journal, December 1960

A primitive pathway of porphyrin biosynthesis and enzymology in Desulfovibrio vulgaris
journal, April 1998

  • Ishida, T.; Yu, L.; Akutsu, H.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 9
  • DOI: 10.1073/pnas.95.9.4853

Molecular Phylogeny and Intricate Evolutionary History of the Three Isofunctional Enzymes Involved in the Oxidation of Protoporphyrinogen IX
journal, August 2014

  • Kobayashi, Koichi; Masuda, Tatsuru; Tajima, Naoyuki
  • Genome Biology and Evolution, Vol. 6, Issue 8
  • DOI: 10.1093/gbe/evu170

Coproporphyrinogenase activities in extracts of Rhodopseudomonas spheroides and Chromatium strain D
journal, August 1972

Substrate interactions with human ferrochelatase
journal, January 2007

  • Medlock, A.; Swartz, L.; Dailey, T. A.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 6
  • DOI: 10.1073/pnas.0606144104

Lack of heme synthesis in a free-living eukaryote
journal, March 2005

  • Rao, A. U.; Carta, L. K.; Lesuisse, E.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 12
  • DOI: 10.1073/pnas.0500877102

Identification of [2Fe-2S] Clusters in Microbial Ferrochelatases
journal, May 2002

Identification of a gene essential for protoporphyrinogen IX oxidase activity in the cyanobacterium Synechocystis sp. PCC6803
journal, September 2010

  • Kato, K.; Tanaka, R.; Sano, S.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 38
  • DOI: 10.1073/pnas.1000771107

One ring to rule them all: Trafficking of heme and heme synthesis intermediates in the metazoans
journal, September 2012

  • Hamza, Iqbal; Dailey, Harry A.
  • Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1823, Issue 9
  • DOI: 10.1016/j.bbamcr.2012.04.009

Biosynthesis of the pigments of life: formation of the macrocycle
journal, May 1980

  • Battersby, Alan R.; Fookes, Christopher J. R.; Matcham, George W. J.
  • Nature, Vol. 285, Issue 5759
  • DOI: 10.1038/285017a0

A whole genome view of prokaryotic haem biosynthesis
journal, August 2002

Structural basis for tetrapyrrole coordination by uroporphyrinogen decarboxylase
journal, December 2003

Purification and Initial Biochemical Characterization of ATP:Cob(I)alamin Adenosyltransferase (EutT) Enzyme of Salmonella enterica
journal, June 2006

  • Buan, Nicole R.; Escalante-Semerena, Jorge C.
  • Journal of Biological Chemistry, Vol. 281, Issue 25
  • DOI: 10.1074/jbc.M603069200

Identification of an FAD Superfamily Containing Protoporphyrinogen Oxidases, Monoamine Oxidases, and Phytoene Desaturase
journal, May 1998

Expression of a cloned protoporphyrinogen oxidase.
journal, January 1994

The iron and Porphyrin Metabolism of Micrococcus Lysodeikticus
journal, February 1957

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    Genome-scale metabolic model analysis indicates low energy production efficiency in marine ammonia-oxidizing archaea
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