Ecological roles of dominant and rare prokaryotes in acid mine drainage revealed by metagenomics and metatranscriptomics
Abstract
Here we report that high-throughput sequencing is expanding our knowledge of microbial diversity in the environment. Still, understanding the metabolic potentials and ecological roles of rare and uncultured microbes in natural communities remains a major challenge. To this end, we applied a ‘divide and conquer’ strategy that partitioned a massive metagenomic data set (>100 Gbp) into subsets based on K-mer frequency in sequence assembly to a low-diversity acid mine drainage (AMD) microbial community and, by integrating with an additional metatranscriptomic assembly, successfully obtained 11 draft genomes most of which represent yet uncultured and/or rare taxa (relative abundance <1%). We report the first genome of a naturally occurring Ferrovum population (relative abundance >90%) and its metabolic potentials and gene expression profile, providing initial molecular insights into the ecological role of these lesser known, but potentially important, microorganisms in the AMD environment. Gene transcriptional analysis of the active taxa revealed major metabolic capabilities executed in situ, including carbon- and nitrogen-related metabolisms associated with syntrophic interactions, iron and sulfur oxidation, which are key in energy conservation and AMD generation, and the mechanisms of adaptation and response to the environmental stresses (heavy metals, low pH and oxidative stress). Remarkably, nitrogen fixation and sulfur oxidationmore »
- Authors:
-
- Sun Yat-Sen Univ., Guangzhou, (China). School of Chemistry and Chemical Engineering. College of Ecology and Evolution
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Publication Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE; National Natural Science Foundation of China (NSFC)
- OSTI Identifier:
- 1263515
- Grant/Contract Number:
- 4093212; U1201233; 31370154
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The ISME Journal
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 6; Journal ID: ISSN 1751-7362
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Hua, Zheng-Shuang, Han, Yu-Jiao, Chen, Lin-Xing, Liu, Jun, Hu, Min, Li, Sheng-Jin, Kuang, Jia-Liang, Chain, Patrick SG, Huang, Li-Nan, and Shu, Wen-Sheng. Ecological roles of dominant and rare prokaryotes in acid mine drainage revealed by metagenomics and metatranscriptomics. United States: N. p., 2014.
Web. doi:10.1038/ismej.2014.212.
Hua, Zheng-Shuang, Han, Yu-Jiao, Chen, Lin-Xing, Liu, Jun, Hu, Min, Li, Sheng-Jin, Kuang, Jia-Liang, Chain, Patrick SG, Huang, Li-Nan, & Shu, Wen-Sheng. Ecological roles of dominant and rare prokaryotes in acid mine drainage revealed by metagenomics and metatranscriptomics. United States. https://doi.org/10.1038/ismej.2014.212
Hua, Zheng-Shuang, Han, Yu-Jiao, Chen, Lin-Xing, Liu, Jun, Hu, Min, Li, Sheng-Jin, Kuang, Jia-Liang, Chain, Patrick SG, Huang, Li-Nan, and Shu, Wen-Sheng. Fri .
"Ecological roles of dominant and rare prokaryotes in acid mine drainage revealed by metagenomics and metatranscriptomics". United States. https://doi.org/10.1038/ismej.2014.212. https://www.osti.gov/servlets/purl/1263515.
@article{osti_1263515,
title = {Ecological roles of dominant and rare prokaryotes in acid mine drainage revealed by metagenomics and metatranscriptomics},
author = {Hua, Zheng-Shuang and Han, Yu-Jiao and Chen, Lin-Xing and Liu, Jun and Hu, Min and Li, Sheng-Jin and Kuang, Jia-Liang and Chain, Patrick SG and Huang, Li-Nan and Shu, Wen-Sheng},
abstractNote = {Here we report that high-throughput sequencing is expanding our knowledge of microbial diversity in the environment. Still, understanding the metabolic potentials and ecological roles of rare and uncultured microbes in natural communities remains a major challenge. To this end, we applied a ‘divide and conquer’ strategy that partitioned a massive metagenomic data set (>100 Gbp) into subsets based on K-mer frequency in sequence assembly to a low-diversity acid mine drainage (AMD) microbial community and, by integrating with an additional metatranscriptomic assembly, successfully obtained 11 draft genomes most of which represent yet uncultured and/or rare taxa (relative abundance <1%). We report the first genome of a naturally occurring Ferrovum population (relative abundance >90%) and its metabolic potentials and gene expression profile, providing initial molecular insights into the ecological role of these lesser known, but potentially important, microorganisms in the AMD environment. Gene transcriptional analysis of the active taxa revealed major metabolic capabilities executed in situ, including carbon- and nitrogen-related metabolisms associated with syntrophic interactions, iron and sulfur oxidation, which are key in energy conservation and AMD generation, and the mechanisms of adaptation and response to the environmental stresses (heavy metals, low pH and oxidative stress). Remarkably, nitrogen fixation and sulfur oxidation were performed by the rare taxa, indicating their critical roles in the overall functioning and assembly of the AMD community. Finally, our study demonstrates the potential of the ‘divide and conquer’ strategy in high-throughput sequencing data assembly for genome reconstruction and functional partitioning analysis of both dominant and rare species in natural microbial assemblages.},
doi = {10.1038/ismej.2014.212},
journal = {The ISME Journal},
number = 6,
volume = 9,
place = {United States},
year = {Fri Nov 07 00:00:00 EST 2014},
month = {Fri Nov 07 00:00:00 EST 2014}
}
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Community transcriptomics reveals universal patterns of protein sequence conservation in natural microbial communities
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