Atmospheric solids analysis probe mass spectrometry for the rapid identification of pollens and semi-quantification of flavonoid fingerprints

Xiao, Xiaoyin; Miller, Lance L.; Parchert, Kylea J.; Hayes, Dulce; Hochrein, James M.

doi:10.1002/rcm.7601

Title: Atmospheric solids analysis probe mass spectrometry for the rapid identification of pollens and semi-quantification of flavonoid fingerprints

Abstract

From allergies to plant reproduction, pollens have important impacts on the health of human and plant populations, yet identification of pollen grains remains difficult and time-consuming. Low-volatility flavonoids generated from pollens cannot be easily characterized and quantified with current analytical techniques. Here we demonstrate the novel use of atmospheric solids analysis probe mass spectrometry (ASAP-MS) for the characterization of flavonoids in pollens. Flavonoid patterns were generated for pollens collected from different plant types (trees and bushes) in addition to bee pollens from distinct geographic regions. Standard flavonoids (kaempferol and rhamnazin) and those produced from pollens were compared and assessed with ASAP-MS using low-energy collision MS/MS. Results for a semi-quantitative method for assessing the amount of a flavonoid in pollens are also presented.

Authors:

Xiao, Xiaoyin ^[1]; Miller, Lance L. ^[1]; Parchert, Kylea J. ^[1]; Hayes, Dulce ^[1]; Hochrein, James M. ^[1]

Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Publication Date:: Wed Jun 08 00:00:00 EDT 2016

Research Org.:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Org.:: USDOE National Nuclear Security Administration (NNSA)

OSTI Identifier:: 1259863

Report Number(s):: SAND-2015-5271J
Journal ID: ISSN 0951-4198; 594518

Grant/Contract Number:: AC04-94AL85000

Resource Type:: Accepted Manuscript

Journal Name:: Rapid Communications in Mass Spectrometry

Additional Journal Information:: Journal Volume: 30; Journal Issue: 13; Journal ID: ISSN 0951-4198

Publisher:: Wiley

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Xiao, Xiaoyin, Miller, Lance L., Parchert, Kylea J., Hayes, Dulce, and Hochrein, James M. Atmospheric solids analysis probe mass spectrometry for the rapid identification of pollens and semi-quantification of flavonoid fingerprints.  United States: N. p., 2016. 
Web.  doi:10.1002/rcm.7601.

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                    Xiao, Xiaoyin, Miller, Lance L., Parchert, Kylea J., Hayes, Dulce, & Hochrein, James M. Atmospheric solids analysis probe mass spectrometry for the rapid identification of pollens and semi-quantification of flavonoid fingerprints.  United States.  https://doi.org/10.1002/rcm.7601

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                    Xiao, Xiaoyin, Miller, Lance L., Parchert, Kylea J., Hayes, Dulce, and Hochrein, James M. Wed .  
"Atmospheric solids analysis probe mass spectrometry for the rapid identification of pollens and semi-quantification of flavonoid fingerprints".  United States.  https://doi.org/10.1002/rcm.7601.  https://www.osti.gov/servlets/purl/1259863.

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@article{osti_1259863,

  title        = {Atmospheric solids analysis probe mass spectrometry for the rapid identification of pollens and semi-quantification of flavonoid fingerprints},

  author       = {Xiao, Xiaoyin and Miller, Lance L. and Parchert, Kylea J. and Hayes, Dulce and Hochrein, James M.},

  abstractNote = {From allergies to plant reproduction, pollens have important impacts on the health of human and plant populations, yet identification of pollen grains remains difficult and time-consuming. Low-volatility flavonoids generated from pollens cannot be easily characterized and quantified with current analytical techniques. Here we demonstrate the novel use of atmospheric solids analysis probe mass spectrometry (ASAP-MS) for the characterization of flavonoids in pollens. Flavonoid patterns were generated for pollens collected from different plant types (trees and bushes) in addition to bee pollens from distinct geographic regions. Standard flavonoids (kaempferol and rhamnazin) and those produced from pollens were compared and assessed with ASAP-MS using low-energy collision MS/MS. Results for a semi-quantitative method for assessing the amount of a flavonoid in pollens are also presented.},

  doi          = {10.1002/rcm.7601},

  journal      = {Rapid Communications in Mass Spectrometry},

  number       = 13,

  volume       = 30,

  place        = {United States},

  year         = {Wed Jun 08 00:00:00 EDT 2016},

  month        = {Wed Jun 08 00:00:00 EDT 2016}

}

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https://doi.org/10.1002/rcm.7601

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Title: Atmospheric solids analysis probe mass spectrometry for the rapid identification of pollens and semi-quantification of flavonoid fingerprints

Abstract

Citation Formats

Atmospheric solids analysis probe: a rapid ionization technique for small molecule drugs journal, November 2008

Mass Spectrometric Investigation of Molecular Variability of Grass Pollen Group 1 Allergens journal, July 2009

Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization journal, October 2004

MALDI-MS Direct Tissue Analysis of Proteins: Improving Signal Sensitivity Using Organic Treatments journal, September 2006

A tandem mass spectrometric study of selected characteristic flavonoids journal, September 2001

New Method for Pollen Identification by FT-IR Spectroscopy journal, January 2003

Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization journal, October 2004

Imaging mass spectrometry journal, January 2007

Mass Spectrometry: Recent Advances in Direct Open Air Surface Sampling/Ionization journal, January 2013

Ambient Sampling/Ionization Mass Spectrometry: Applications and Current Trends journal, June 2011

Atmospheric solids analysis probe: a rapid ionization technique for small molecule drugs journal, November 2008

Evaluating Atmospheric pressure Solids Analysis Probe (ASAP) mass spectrometry for the analysis of low molecular weight synthetic polymers journal, January 2012

Visualizing life with ambient mass spectrometry journal, February 2015

Analysis of Solids, Liquids, and Biological Tissues Using Solids Probe Introduction at Atmospheric Pressure on Commercial LC/MS Instruments journal, December 2005

A New Matrix Assisted Ionization Method for the Analysis of Volatile and Nonvolatile Compounds by Atmospheric Probe Mass Spectrometry journal, May 2013

Measurement of Vapor Pressures and Heats of Sublimation of Dicarboxylic Acids Using Atmospheric Solids Analysis Probe Mass Spectrometry journal, April 2012

Molecular Ions and Protonated Molecules Observed in the Atmospheric Solids Analysis Probe Analysis of Steroids journal, February 2010

An assessment of atmospheric-pressure solids-analysis probes for the detection of chemicals in food journal, December 2010

Atmospheric Solid Analysis Probe–Ion Mobility Mass Spectrometry of Polypropylene journal, September 2012

Pollen and spores: Microscopic keys to understanding the earth's biodiversity journal, December 2006

Development of a Proxy for Past Surface UV-B Irradiation: A Thermally Assisted Hydrolysis and Methylation py-GC/MS Method for the Analysis of Pollen and Spores journal, August 2005

New Method for Pollen Identification by FT-IR Spectroscopy journal, January 2003

Chemical Characterization and Classification of Pollen journal, December 2008

Characterization and discrimination of pollen by Raman microscopy journal, December 2004

Surface-Enhanced Raman Spectroscopy of Bacteria and Pollen journal, August 2005

Identification of phenolic compounds from pollen extracts using capillary electrophoresis–electrospray time-of-flight mass spectrometry journal, September 2007

Pollen Lipidomics: Lipid Profiling Exposes a Notable Diversity in 22 Allergenic Pollen and Potential Biomarkers of the Allergic Immune Response journal, February 2013

Proteomic profiling of birch ( Betula verrucosa ) pollen extracts from different origins journal, February 2011

Matrix-assisted laser desorption/ionization mass spectrometric investigation of pollen and their classification by multivariate statistics journal, March 2012

Mass Spectrometric Investigation of Molecular Variability of Grass Pollen Group 1 Allergens journal, July 2009

Matrix-assisted laser desorption/ionization imaging mass spectrometry of pollen grains and their mixtures journal, March 2013

Characterization of pollen by MALDI-TOF lipid profiling journal, January 2013

Thermal degradation of β-carotene studied by ion mobility atmospheric solid analysis probe mass spectrometry: full product pattern and selective ionization enhancement: Degradation of low volatility compounds journal, March 2016

Flavonoids: Prospective Drug Candidates journal, November 2008

Therapeutic potential of inhibition of the NF-κB pathway in the treatment of inflammation and cancer journal, January 2001

Recent advances in understanding the anti-diabetic actions of dietary flavonoids journal, November 2013

A fragmentation study of kaempferol using electrospray quadrupole time-of-flight mass spectrometry at high mass resolution journal, February 2004

Mass spectrometry in the structural analysis of flavonoids journal, January 2004

Atmospheric solids analysis probe: a rapid ionization technique for small molecule drugs
journal, November 2008

Mass Spectrometric Investigation of Molecular Variability of Grass Pollen Group 1 Allergens
journal, July 2009

Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization
journal, October 2004

MALDI-MS Direct Tissue Analysis of Proteins: Improving Signal Sensitivity Using Organic Treatments
journal, September 2006

A tandem mass spectrometric study of selected characteristic flavonoids
journal, September 2001

New Method for Pollen Identification by FT-IR Spectroscopy
journal, January 2003

Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization
journal, October 2004

Imaging mass spectrometry
journal, January 2007

Mass Spectrometry: Recent Advances in Direct Open Air Surface Sampling/Ionization
journal, January 2013

Ambient Sampling/Ionization Mass Spectrometry: Applications and Current Trends
journal, June 2011

Atmospheric solids analysis probe: a rapid ionization technique for small molecule drugs
journal, November 2008

Evaluating Atmospheric pressure Solids Analysis Probe (ASAP) mass spectrometry for the analysis of low molecular weight synthetic polymers
journal, January 2012

Visualizing life with ambient mass spectrometry
journal, February 2015

Analysis of Solids, Liquids, and Biological Tissues Using Solids Probe Introduction at Atmospheric Pressure on Commercial LC/MS Instruments
journal, December 2005

A New Matrix Assisted Ionization Method for the Analysis of Volatile and Nonvolatile Compounds by Atmospheric Probe Mass Spectrometry
journal, May 2013

Measurement of Vapor Pressures and Heats of Sublimation of Dicarboxylic Acids Using Atmospheric Solids Analysis Probe Mass Spectrometry
journal, April 2012

Molecular Ions and Protonated Molecules Observed in the Atmospheric Solids Analysis Probe Analysis of Steroids
journal, February 2010

An assessment of atmospheric-pressure solids-analysis probes for the detection of chemicals in food
journal, December 2010

Atmospheric Solid Analysis Probe–Ion Mobility Mass Spectrometry of Polypropylene
journal, September 2012

Pollen and spores: Microscopic keys to understanding the earth's biodiversity
journal, December 2006

Development of a Proxy for Past Surface UV-B Irradiation: A Thermally Assisted Hydrolysis and Methylation py-GC/MS Method for the Analysis of Pollen and Spores
journal, August 2005

New Method for Pollen Identification by FT-IR Spectroscopy
journal, January 2003

Chemical Characterization and Classification of Pollen
journal, December 2008

Characterization and discrimination of pollen by Raman microscopy
journal, December 2004

Surface-Enhanced Raman Spectroscopy of Bacteria and Pollen
journal, August 2005

Identification of phenolic compounds from pollen extracts using capillary electrophoresis–electrospray time-of-flight mass spectrometry
journal, September 2007

Pollen Lipidomics: Lipid Profiling Exposes a Notable Diversity in 22 Allergenic Pollen and Potential Biomarkers of the Allergic Immune Response
journal, February 2013

Proteomic profiling of birch ( Betula verrucosa ) pollen extracts from different origins
journal, February 2011

Matrix-assisted laser desorption/ionization mass spectrometric investigation of pollen and their classification by multivariate statistics
journal, March 2012

Mass Spectrometric Investigation of Molecular Variability of Grass Pollen Group 1 Allergens
journal, July 2009

Matrix-assisted laser desorption/ionization imaging mass spectrometry of pollen grains and their mixtures
journal, March 2013

Characterization of pollen by MALDI-TOF lipid profiling
journal, January 2013

Thermal degradation of β-carotene studied by ion mobility atmospheric solid analysis probe mass spectrometry: full product pattern and selective ionization enhancement: Degradation of low volatility compounds
journal, March 2016

Flavonoids: Prospective Drug Candidates
journal, November 2008

Therapeutic potential of inhibition of the NF-κB pathway in the treatment of inflammation and cancer
journal, January 2001

Recent advances in understanding the anti-diabetic actions of dietary flavonoids
journal, November 2013

A fragmentation study of kaempferol using electrospray quadrupole time-of-flight mass spectrometry at high mass resolution
journal, February 2004

Mass spectrometry in the structural analysis of flavonoids
journal, January 2004