Identification of a Novel Drug Lead That Inhibits HCV Infection and Cell-to-Cell Transmission by Targeting the HCV E2 Glycoprotein
Abstract
We report that Hepatitis C Virus (HCV) infects 200 million individuals worldwide. Although several FDA approved drugs targeting the HCV serine protease and polymerase have shown promising results, there is a need for better drugs that are effective in treating a broader range of HCV genotypes and subtypes without being used in combination with interferon and/or ribavirin. Recently, two crystal structures of the core of the HCV E2 protein (E2c) have been determined, providing structural information that can now be used to target the E2 protein and develop drugs that disrupt the early stages of HCV infection by blocking E2’s interaction with different host factors. Using the E2c structure as a template, we have created a structural model of the E2 protein core (residues 421–645) that contains the three amino acid segments that are not present in either structure. Computational docking of a diverse library of 1,715 small molecules to this model led to the identification of a set of 34 ligands predicted to bind near conserved amino acid residues involved in the HCV E2: CD81 interaction. We used surface plasmon resonance detection to screen the ligand set for binding to recombinant E2 protein, and the best binders were subsequentlymore »
- Authors:
-
- American University in Cairo, New Cairo (Egypt)
- Univ. of Lille North of France, Lille (France)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- California Inst. of Technology (CalTech), Pasadena, CA (United States)
- Rutgers Univ., Piscataway, NJ (United States)
- Stanford Univ. Medical Center, Stanford, CA (United States)
- Rutgers University-New Jersey Medical School, Newark, NJ (United States)
- Scripps Research Inst., La Jolla, FL (United States)
- Univ. of California, Davis, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE; American University in Cairo, Egypt
- OSTI Identifier:
- 1259520
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 10; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Al Olaby, Reem R., Cocquerel, Laurence, Zemla, Adam, Saas, Laure, Dubuisson, Jean, Vielmetter, Jost, Marcotrigiano, Joseph, Khan, Abdul Ghafoor, Catalan, Felipe Vences, Perryman, Alexander L., Freundlich, Joel S., Forli, Stefano, Levy, Shoshana, Balhorn, Rod, and Azzazy, Hassan M. Identification of a Novel Drug Lead That Inhibits HCV Infection and Cell-to-Cell Transmission by Targeting the HCV E2 Glycoprotein. United States: N. p., 2014.
Web. doi:10.1371/journal.pone.0111333.
Al Olaby, Reem R., Cocquerel, Laurence, Zemla, Adam, Saas, Laure, Dubuisson, Jean, Vielmetter, Jost, Marcotrigiano, Joseph, Khan, Abdul Ghafoor, Catalan, Felipe Vences, Perryman, Alexander L., Freundlich, Joel S., Forli, Stefano, Levy, Shoshana, Balhorn, Rod, & Azzazy, Hassan M. Identification of a Novel Drug Lead That Inhibits HCV Infection and Cell-to-Cell Transmission by Targeting the HCV E2 Glycoprotein. United States. https://doi.org/10.1371/journal.pone.0111333
Al Olaby, Reem R., Cocquerel, Laurence, Zemla, Adam, Saas, Laure, Dubuisson, Jean, Vielmetter, Jost, Marcotrigiano, Joseph, Khan, Abdul Ghafoor, Catalan, Felipe Vences, Perryman, Alexander L., Freundlich, Joel S., Forli, Stefano, Levy, Shoshana, Balhorn, Rod, and Azzazy, Hassan M. Thu .
"Identification of a Novel Drug Lead That Inhibits HCV Infection and Cell-to-Cell Transmission by Targeting the HCV E2 Glycoprotein". United States. https://doi.org/10.1371/journal.pone.0111333. https://www.osti.gov/servlets/purl/1259520.
@article{osti_1259520,
title = {Identification of a Novel Drug Lead That Inhibits HCV Infection and Cell-to-Cell Transmission by Targeting the HCV E2 Glycoprotein},
author = {Al Olaby, Reem R. and Cocquerel, Laurence and Zemla, Adam and Saas, Laure and Dubuisson, Jean and Vielmetter, Jost and Marcotrigiano, Joseph and Khan, Abdul Ghafoor and Catalan, Felipe Vences and Perryman, Alexander L. and Freundlich, Joel S. and Forli, Stefano and Levy, Shoshana and Balhorn, Rod and Azzazy, Hassan M.},
abstractNote = {We report that Hepatitis C Virus (HCV) infects 200 million individuals worldwide. Although several FDA approved drugs targeting the HCV serine protease and polymerase have shown promising results, there is a need for better drugs that are effective in treating a broader range of HCV genotypes and subtypes without being used in combination with interferon and/or ribavirin. Recently, two crystal structures of the core of the HCV E2 protein (E2c) have been determined, providing structural information that can now be used to target the E2 protein and develop drugs that disrupt the early stages of HCV infection by blocking E2’s interaction with different host factors. Using the E2c structure as a template, we have created a structural model of the E2 protein core (residues 421–645) that contains the three amino acid segments that are not present in either structure. Computational docking of a diverse library of 1,715 small molecules to this model led to the identification of a set of 34 ligands predicted to bind near conserved amino acid residues involved in the HCV E2: CD81 interaction. We used surface plasmon resonance detection to screen the ligand set for binding to recombinant E2 protein, and the best binders were subsequently tested to identify compounds that inhibit the infection of Huh-7 cells by HCV. One compound, 281816, blocked E2 binding to CD81 and inhibited HCV infection in a genotype-independent manner with IC50’s ranging from 2.2 µM to 4.6 µM. 281816 blocked the early and late steps of cell-free HCV entry and also abrogated the cell-to-cell transmission of HCV. Collectively the results obtained with this new structural model of E2c suggest the development of small molecule inhibitors such as 281816 that target E2 and disrupt its interaction with CD81 may provide a new paradigm for HCV treatment.},
doi = {10.1371/journal.pone.0111333},
journal = {PLoS ONE},
number = 10,
volume = 9,
place = {United States},
year = {Thu Oct 30 00:00:00 EDT 2014},
month = {Thu Oct 30 00:00:00 EDT 2014}
}
Web of Science
Works referenced in this record:
Robust production of infectious viral particles in Huh-7 cells by introducing mutations in hepatitis C virus structural proteins
journal, September 2007
- Delgrange, D.; Pillez, A.; Castelain, S.
- Journal of General Virology, Vol. 88, Issue 9
Virologic response and characterisation of HCV genotype 2–6 in patients receiving TMC435 monotherapy (study TMC435-C202)
journal, March 2013
- Lenz, Oliver; Vijgen, Leen; Berke, Jan Martin
- Journal of Hepatology, Vol. 58, Issue 3
Hepatitis C Virus E2 Envelope Glycoprotein Core Structure
journal, November 2013
- Kong, L.; Giang, E.; Nieusma, T.
- Science, Vol. 342, Issue 6162
Mutation of juxtamembrane cysteines in the tetraspanin CD81 affects palmitoylation and alters interaction with other proteins at the cell surface
journal, July 2009
- Delandre, Caroline; Penabaz, Taryn R.; Passarelli, A. Lorena
- Experimental Cell Research, Vol. 315, Issue 11
AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility
journal, December 2009
- Morris, Garrett M.; Huey, Ruth; Lindstrom, William
- Journal of Computational Chemistry, Vol. 30, Issue 16
Improved prediction of protein side-chain conformations with SCWRL4
journal, December 2009
- Krivov, Georgii G.; Shapovalov, Maxim V.; Dunbrack, Roland L.
- Proteins: Structure, Function, and Bioinformatics, Vol. 77, Issue 4
CD81 is dispensable for hepatitis C virus cell-to-cell transmission in hepatoma cells
journal, January 2009
- Witteveldt, J.; Evans, M. J.; Bitzegeio, J.
- Journal of General Virology, Vol. 90, Issue 1
Safety and on-treatment efficacy of telaprevir: the early access programme for patients with advanced hepatitis C
journal, November 2013
- Colombo, M.; Fernández, I.; Abdurakhmanov, D.
- Gut, Vol. 63, Issue 7
Sofosbuvir for the treatment of hepatitis C virus
journal, October 2013
- Asselah, Tarik
- Expert Opinion on Pharmacotherapy, Vol. 15, Issue 1
Virtual screening with AutoDock Vina and the common pharmacophore engine of a low diversity library of fragments and hits against the three allosteric sites of HIV integrase: participation in the SAMPL4 protein–ligand binding challenge
journal, February 2014
- Perryman, Alexander L.; Santiago, Daniel N.; Forli, Stefano
- Journal of Computer-Aided Molecular Design, Vol. 28, Issue 4
Patterns and Trends in Antipsychotic Prescribing for Parkinson Disease Psychosis
journal, July 2011
- Weintraub, Daniel
- Archives of Neurology, Vol. 68, Issue 7
Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates
journal, August 2002
- Sandrin, Virginie; Boson, Bertrand; Salmon, Patrick
- Blood, Vol. 100, Issue 3
Identification of Conserved Residues in the E2 Envelope Glycoprotein of the Hepatitis C Virus That Are Critical for CD81 Binding
journal, August 2006
- Owsianka, A. M.; Timms, J. M.; Tarr, A. W.
- Journal of Virology, Vol. 80, Issue 17
RNA Interference and Single Particle Tracking Analysis of Hepatitis C Virus Endocytosis
journal, December 2009
- Coller, Kelly E.; Berger, Kristi L.; Heaton, Nicholas S.
- PLoS Pathogens, Vol. 5, Issue 12
AS2TS system for protein structure modeling and analysis
journal, July 2005
- Zemla, A.; Zhou, C. E.; Slezak, T.
- Nucleic Acids Research, Vol. 33, Issue Web Server
CD81 and Hepatitis C Virus (HCV) Infection
journal, February 2014
- Fénéant, Lucie; Levy, Shoshana; Cocquerel, Laurence
- Viruses, Vol. 6, Issue 2
Biogenesis of hepatitis C virus envelope glycoproteins
journal, November 2001
- Cocquerel, Laurence; Op De Beeck, Anne; Dubuisson, Jean
- Journal of General Virology, Vol. 82, Issue 11
Structural properties of the ectodomain of hepatitis C virus E2 envelope protein
journal, January 2009
- Rodríguez-Rodríguez, Mar; Tello, Daniel; Yélamos, Belén
- Virus Research, Vol. 139, Issue 1
Characterisation of bacterially expressed structural protein E2 of hepatitis C virus
journal, September 2004
- Yurkova, Maria S.; Patel, Arvind H.; Fedorov, Alexey N.
- Protein Expression and Purification, Vol. 37, Issue 1
Pattern of expression of tetraspanin antigen genes in Burkitt lymphoma cell lines
journal, December 2001
- Ferrer, M.; Yunta, M.; Lazo, P. A.
- Clinical and Experimental Immunology, Vol. 113, Issue 3, p. 346-352
(−)-Epigallocatechin-3-gallate is a new inhibitor of hepatitis C virus entry
journal, February 2012
- Calland, Noémie; Albecka, Anna; Belouzard, Sandrine
- Hepatology, Vol. 55, Issue 3
Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Proteins Incorporated into Infectious Virions
journal, September 2010
- Helle, F.; Vieyres, G.; Elkrief, L.
- Journal of Virology, Vol. 84, Issue 22
Exosome-mediated transmission of hepatitis C virus between human hepatoma Huh7.5 cells
journal, July 2013
- Ramakrishnaiah, V.; Thumann, C.; Fofana, I.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 32
Advantages of a single-cycle production assay to study cell culture-adaptive mutations of hepatitis C virus
journal, March 2008
- Russell, R. S.; Meunier, J. -C.; Takikawa, S.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 11
Expression, purification, immunological characterization and application of Escherichia coli-derived hepatitis C virus E2 proteins
journal, October 2001
- Liu, Jing; Zhu, Lixin; Zhang, Xinxin
- Biotechnology and Applied Biochemistry, Vol. 34, Issue 2
The Transmembrane Domains of Hepatitis C Virus Envelope Glycoproteins E1 and E2 Play a Major Role in Heterodimerization
journal, May 2000
- Op De Beeck, Anne; Montserret, Roland; Duvet, Sandrine
- Journal of Biological Chemistry, Vol. 275, Issue 40
Delineation of Regions Important for Heteromeric Association of Hepatitis C Virus E1 and E2
journal, April 1997
- Yi, Minkyung; Nakamoto, Yasunari; Kaneko, Shuichi
- Virology, Vol. 231, Issue 1
Broadly neutralizing antibodies protect against hepatitis C virus quasispecies challenge
journal, December 2007
- Law, Mansun; Maruyama, Toshiaki; Lewis, Jamie
- Nature Medicine, Vol. 14, Issue 1
The Natural Killer Cell Response to HCV Infection
journal, January 2013
- Ahlenstiel, Golo
- Immune Network, Vol. 13, Issue 5
The Neutralizing Activity of Anti-Hepatitis C Virus Antibodies Is Modulated by Specific Glycans on the E2 Envelope Protein
journal, May 2007
- Helle, F.; Goffard, A.; Morel, V.
- Journal of Virology, Vol. 81, Issue 15
Short-Range Exosomal Transfer of Viral RNA from Infected Cells to Plasmacytoid Dendritic Cells Triggers Innate Immunity
journal, October 2012
- Dreux, Marlène; Garaigorta, Urtzi; Boyd, Bryan
- Cell Host & Microbe, Vol. 12, Issue 4
Folding and dimerization of hepatitis C virus E1 and E2 glycoproteins in stably transfected CHO cells
journal, February 2005
- Brazzoli, Michela; Helenius, Ari; Foung, Steven K. H.
- Virology, Vol. 332, Issue 1
EWI-2wint promotes CD81 clustering that abrogates Hepatitis C Virus entry: Dynamics and partitioning of CD81
journal, February 2013
- Potel, Julie; Rassam, Patrice; Montpellier, Claire
- Cellular Microbiology, Vol. 15, Issue 7
Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system
journal, January 2010
- Jones, Christopher T.; Catanese, Maria Teresa; Law, Lok Man J.
- Nature Biotechnology, Vol. 28, Issue 2
Analysis of Hepatitis C Virus Capsid, E1, and E2/NS1 Proteins Expressed in Insect Cells
journal, November 1993
- Lanford, Robert E.; Notvall, Lena; Chavez, Deborah
- Virology, Vol. 197, Issue 1
In vitro assay for neutralizing antibody to hepatitis C virus: Evidence for broadly conserved neutralization epitopes
journal, November 2003
- Bartosch, B.; Bukh, J.; Meunier, J. -C.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 24
The antimalarial ferroquine is an inhibitor of hepatitis C virus
journal, May 2013
- Vausselin, Thibaut; Calland, Noémie; Belouzard, Sandrine
- Hepatology, Vol. 58, Issue 1
Production of infectious hepatitis C virus in tissue culture from a cloned viral genome
journal, June 2005
- Wakita, Takaji; Pietschmann, Thomas; Kato, Takanobu
- Nature Medicine, Vol. 11, Issue 7
Systematic Studies with Amoxapine,
a New Antidepressant
journal, January 1982
- Ban, T. A.; Fujimori, M.; Petrie, W. M.
- International Pharmacopsychiatry, Vol. 17, Issue 1
Characterization of Antibodies Induced by Vaccination with Hepatitis C Virus Envelope Glycoproteins
journal, September 2010
- Ray, Ranjit; Meyer, Keith; Banerjee, Arup
- The Journal of Infectious Diseases, Vol. 202, Issue 6
Dissecting the role of putative CD81 binding regions of E2 in mediating HCV entry: Putative CD81 binding region 1 is not involved in CD81 binding
journal, January 2008
- Rothwangl, Katharina B.; Manicassamy, Balaji; Uprichard, Susan L.
- Virology Journal, Vol. 5, Issue 1
Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2
journal, February 2014
- Khan, Abdul Ghafoor; Whidby, Jillian; Miller, Matthew T.
- Nature, Vol. 509, Issue 7500
Rapid acute treatment of agitation in patients with bipolar I disorder: a multicenter, randomized, placebo-controlled clinical trial with inhaled loxapine: Inhaled loxapine for bipolar disorder
journal, February 2012
- Kwentus, Joseph; Riesenberg, Robert A.; Marandi, Morteza
- Bipolar Disorders, Vol. 14, Issue 1
A quantitative test to estimate neutralizing antibodies to the hepatitis C virus: cytofluorimetric assessment of envelope glycoprotein 2 binding to target cells.
journal, March 1996
- Rosa, D.; Campagnoli, S.; Moretto, C.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 5
High-Level Expression, Single-Step Immunoaffinity Purification and Characterization of Human Tetraspanin Membrane Protein CD81
journal, June 2008
- Takayama, Hidehito; Chelikani, Prashen; Reeves, Philip J.
- PLoS ONE, Vol. 3, Issue 6
Different Requirements for Scavenger Receptor Class B Type I in Hepatitis C Virus Cell-Free versus Cell-to-Cell Transmission
journal, May 2013
- Catanese, M. T.; Loureiro, J.; Jones, C. T.
- Journal of Virology, Vol. 87, Issue 15
The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry
journal, April 2008
- Rocha-Perugini, Vera; Montpellier, Claire; Delgrange, David
- PLoS ONE, Vol. 3, Issue 4
Diverse CD81 Proteins Support Hepatitis C Virus Infection
journal, August 2006
- Flint, M.; von Hahn, T.; Zhang, J.
- Journal of Virology, Vol. 80, Issue 22
A Novel Monoclonal Anti-CD81 Antibody Produced by Genetic Immunization Efficiently Inhibits Hepatitis C Virus Cell-Cell Transmission
journal, May 2013
- Fofana, Isabel; Xiao, Fei; Thumann, Christine
- PLoS ONE, Vol. 8, Issue 5
Summary of the Comparative Effectiveness Review on Off-Label Use of Atypical Antipsychotics
journal, June 2012
- Maher, Alicia R.; Theodore, George
- Journal of Managed Care Pharmacy, Vol. 18, Issue 5 Supp B
Acute hepatitis C virus infection: A chronic problem
journal, December 2007
- Blackard, Jason T.; Shata, M. Tarek; Shire, Norah J.
- Hepatology, Vol. 47, Issue 1
Hepatitis C virus cell-cell transmission in hepatoma cells in the presence of neutralizing antibodies
journal, October 2007
- Timpe, Jennifer M.; Stamataki, Zania; Jennings, Adam
- Hepatology, Vol. 47, Issue 1
AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading
journal, January 2009
- Trott, Oleg; Olson, Arthur J.
- Journal of Computational Chemistry
Delineation of Regions Important for Heteromeric Association of Hepatitis C Virus E1 and E2
journal, April 1997
- Yi, Minkyung; Nakamoto, Yasunari; Kaneko, Shuichi
- Virology, Vol. 231, Issue 1
Cianopramine and amitriptyline in the treatment of depressed patients ? a placebo-controlled study
journal, January 1985
- Hormazabal, L.; Omer, L. M. O.; Ismail, S.
- Psychopharmacology, Vol. 86, Issue 1-2
Multimeric HCV E2 protein obtained from pichia pastoris cells induces a strong immune response in mice
journal, March 2007
- Martinez-Donato, Gillian; Capdesuñer, Yanelis; Acosta-Rivero, Nelson
- Molecular Biotechnology, Vol. 35, Issue 3
Virtual screening with AutoDock Vina and the common pharmacophore engine of a low diversity library of fragments and hits against the three allosteric sites of HIV integrase: participation in the SAMPL4 protein–ligand binding challenge
journal, February 2014
- Perryman, Alexander L.; Santiago, Daniel N.; Forli, Stefano
- Journal of Computer-Aided Molecular Design, Vol. 28, Issue 4
CD81 protein is expressed at high levels in normal germinal center B cells and in subtypes of human lymphomas
journal, February 2010
- Luo, Robert F.; Zhao, Shuchun; Tibshirani, Robert
- Human Pathology, Vol. 41, Issue 2
Perspectives for the utilization of neutralizing human monoclonal antibodies as anti-HCV drugs
journal, August 2008
- Burioni, Roberto; Perotti, Mario; Mancini, Nicasio
- Journal of Hepatology, Vol. 49, Issue 2
Virologic response and characterisation of HCV genotype 2–6 in patients receiving TMC435 monotherapy (study TMC435-C202)
journal, March 2013
- Lenz, Oliver; Vijgen, Leen; Berke, Jan Martin
- Journal of Hepatology, Vol. 58, Issue 3
Small molecule inhibition of hepatitis C virus E2 binding to CD81
journal, September 2003
- VanCompernolle, Scott E.; Wiznycia, Alexander V.; Rush, Jeremy R.
- Virology, Vol. 314, Issue 1
The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines
journal, December 1997
- Malich, Gregor; Markovic, Boban; Winder, Chris
- Toxicology, Vol. 124, Issue 3
Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system
journal, January 2010
- Jones, Christopher T.; Catanese, Maria Teresa; Law, Lok Man J.
- Nature Biotechnology, Vol. 28, Issue 2
Production of infectious hepatitis C virus in tissue culture from a cloned viral genome
journal, June 2005
- Wakita, Takaji; Pietschmann, Thomas; Kato, Takanobu
- Nature Medicine, Vol. 11, Issue 7
Pattern of expression of tetraspanin antigen genes in Burkitt lymphoma cell lines
journal, December 2001
- Ferrer, M.; Yunta, M.; Lazo, P. A.
- Clinical and Experimental Immunology, Vol. 113, Issue 3, p. 346-352
In vitro assay for neutralizing antibody to hepatitis C virus: Evidence for broadly conserved neutralization epitopes
journal, November 2003
- Bartosch, B.; Bukh, J.; Meunier, J. -C.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 24
A quantitative test to estimate neutralizing antibodies to the hepatitis C virus: cytofluorimetric assessment of envelope glycoprotein 2 binding to target cells.
journal, March 1996
- Rosa, D.; Campagnoli, S.; Moretto, C.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 5
Hepatitis C Virus Glycoprotein E2 Contains a Membrane-proximal Heptad Repeat Sequence That Is Essential for E1E2 Glycoprotein Heterodimerization and Viral Entry
journal, May 2004
- Drummer, Heidi E.; Poumbourios, Pantelis
- Journal of Biological Chemistry, Vol. 279, Issue 29
Immunization of Human Volunteers With Hepatitis C Virus Envelope Glycoproteins Elicits Antibodies That Cross-Neutralize Heterologous Virus Strains
journal, September 2011
- Stamataki, Zania; Coates, Stephen; Abrignani, Sergio
- The Journal of Infectious Diseases, Vol. 204, Issue 5
CD81 is dispensable for hepatitis C virus cell-to-cell transmission in hepatoma cells
journal, January 2009
- Witteveldt, J.; Evans, M. J.; Bitzegeio, J.
- Journal of General Virology, Vol. 90, Issue 1
Robust production of infectious viral particles in Huh-7 cells by introducing mutations in hepatitis C virus structural proteins
journal, September 2007
- Delgrange, D.; Pillez, A.; Castelain, S.
- Journal of General Virology, Vol. 88, Issue 9
Hepatitis C Virus E2 Envelope Glycoprotein Core Structure
journal, November 2013
- Kong, L.; Giang, E.; Nieusma, T.
- Science, Vol. 342, Issue 6162
Hepatitis C Virus Entry Depends on Clathrin-Mediated Endocytosis
journal, June 2006
- Blanchard, E.; Belouzard, S.; Goueslain, L.
- Journal of Virology, Vol. 80, Issue 14
Characterization of Hepatitis C Virus Recombinants with Chimeric E1/E2 Envelope Proteins and Identification of Single Amino Acids in the E2 Stem Region Important for Entry
journal, November 2012
- Carlsen, Thomas H. R.; Scheel, Troels K. H.; Ramirez, Santseharay
- Journal of Virology, Vol. 87, Issue 3
Blocking Hepatitis C Virus Infection with Recombinant Form of Envelope Protein 2 Ectodomain
journal, August 2009
- Whidby, J.; Mateu, G.; Scarborough, H.
- Journal of Virology, Vol. 83, Issue 21
Characterization of the Envelope Glycoproteins Associated with Infectious Hepatitis C Virus
journal, July 2010
- Vieyres, G.; Thomas, X.; Descamps, V.
- Journal of Virology, Vol. 84, Issue 19
Hepatitis C Virus Entry Requires a Critical Postinternalization Step and Delivery to Early Endosomes via Clathrin-Coated Vesicles
journal, September 2006
- Meertens, L.; Bertaux, C.; Dragic, T.
- Journal of Virology, Vol. 80, Issue 23
Novel Function of CD81 in Controlling Hepatitis C Virus Replication
journal, January 2010
- Zhang, Y. -Y.; Zhang, B. -H.; Ishii, K.
- Journal of Virology, Vol. 84, Issue 7
Characterization of hepatitis C virus envelope glycoprotein complexes expressed by recombinant vaccinia viruses.
journal, January 1993
- Ralston, R.; Thudium, K.; Berger, K.
- Journal of Virology, Vol. 67, Issue 11
Expression and identification of hepatitis C virus polyprotein cleavage products.
journal, January 1993
- Grakoui, A.; Wychowski, C.; Lin, C.
- Journal of Virology, Vol. 67, Issue 3
Formation and intracellular localization of hepatitis C virus envelope glycoprotein complexes expressed by recombinant vaccinia and Sindbis viruses.
journal, January 1994
- Dubuisson, J.; Hsu, H. H.; Cheung, R. C.
- Journal of Virology, Vol. 68, Issue 10
Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin.
journal, January 1996
- Dubuisson, J.; Rice, C. M.
- Journal of virology, Vol. 70, Issue 2
Formation of native hepatitis C virus glycoprotein complexes.
journal, January 1997
- Deleersnyder, V.; Pillez, A.; Wychowski, C.
- Journal of virology, Vol. 71, Issue 1
A Retention Signal Necessary and Sufficient for Endoplasmic Reticulum Localization Maps to the Transmembrane Domain of Hepatitis C Virus Glycoprotein E2
journal, March 1998
- Cocquerel, Laurence; Meunier, Jean-Christophe; Pillez, André
- Journal of Virology, Vol. 72, Issue 3
Characterization of Hepatitis C Virus E2 Glycoprotein Interaction with a Putative Cellular Receptor, CD81
journal, August 1999
- Flint, Mike; Maidens, Catherine; Loomis-Price, Larry D.
- Journal of Virology, Vol. 73, Issue 8
Identification of Amino Acid Residues in CD81 Critical for Interaction with Hepatitis C Virus Envelope Glycoprotein E2
journal, April 2000
- Higginbottom, A.; Quinn, E. R.; Kuo, C. -C.
- Journal of Virology, Vol. 74, Issue 8
Identification of the Hepatitis C Virus E2 Glycoprotein Binding Site on the Large Extracellular Loop of CD81
journal, November 2002
- Drummer, H. E.; Wilson, K. A.; Poumbourios, P.
- Journal of Virology, Vol. 76, Issue 21
Binding of the Hepatitis C Virus E2 Glycoprotein to CD81 Is Strain Specific and Is Modulated by a Complex Interplay between Hypervariable Regions 1 and 2
journal, February 2003
- Roccasecca, RosaMaria; Ansuini, Helenia; Vitelli, Alessandra
- Journal of Virology, Vol. 77, Issue 3
Characterization of Functional Hepatitis C Virus Envelope Glycoproteins
journal, February 2004
- Op De Beeck, A.; Voisset, C.; Bartosch, B.
- Journal of Virology, Vol. 78, Issue 6
Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Glycoproteins
journal, June 2005
- Goffard, A.; Callens, N.; Bartosch, B.
- Journal of Virology, Vol. 79, Issue 13
Building of the Tetraspanin Web: Distinct Structural Domains of CD81 Function in Different Cellular Compartments
journal, January 2006
- Shoham, T.; Rajapaksa, R.; Kuo, C. -C.
- Molecular and Cellular Biology, Vol. 26, Issue 4
Systematic Studies with Amoxapine,
a New Antidepressant
journal, January 1982
- Ban, T. A.; Fujimori, M.; Petrie, W. M.
- International Pharmacopsychiatry, Vol. 17, Issue 1
Prevalence of active hepatitis C virus infections among general public of Lahore, Pakistan
journal, January 2013
- Anwar, Muhammad; Rahman, Moazur; Hassan, Mahmood
- Virology Journal, Vol. 10, Issue 1
Conserved peptides within the E2 region of Hepatitis C virus induce humoral and cellular responses in goats
journal, January 2009
- El-Awady, Mostafa K.; Tabll, Ashraf A.; El-Abd, Yasmine S.
- Virology Journal, Vol. 6, Issue 1
The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry
journal, April 2008
- Rocha-Perugini, Vera; Montpellier, Claire; Delgrange, David
- PLoS ONE, Vol. 3, Issue 4
A Novel Monoclonal Anti-CD81 Antibody Produced by Genetic Immunization Efficiently Inhibits Hepatitis C Virus Cell-Cell Transmission
journal, May 2013
- Fofana, Isabel; Xiao, Fei; Thumann, Christine
- PLoS ONE, Vol. 8, Issue 5
RNA Interference and Single Particle Tracking Analysis of Hepatitis C Virus Endocytosis
journal, December 2009
- Coller, Kelly E.; Berger, Kristi L.; Heaton, Nicholas S.
- PLoS Pathogens, Vol. 5, Issue 12
Sofosbuvir for the treatment of hepatitis C virus
journal, October 2013
- Asselah, Tarik
- Expert Opinion on Pharmacotherapy, Vol. 15, Issue 1
Quetiapine monotherapy for bipolar depression
journal, March 2008
- Thase, Michael
- Neuropsychiatric Disease and Treatment
CD81 and Hepatitis C Virus (HCV) Infection
journal, February 2014
- Fénéant, Lucie; Levy, Shoshana; Cocquerel, Laurence
- Viruses, Vol. 6, Issue 2
Elicitation of strong immune responses by a DNA vaccine expressing a secreted form of hepatitis C virus envelope protein E2 in murine and porcine animal models
journal, January 2006
- Li, Yi-Ping
- World Journal of Gastroenterology, Vol. 12, Issue 44
Efficacy and Safety of Loxapine for Inhalation in the Treatment of Agitation in Patients With Schizophrenia: A Randomized, Double-Blind, Placebo-Controlled Trial
journal, January 2011
- Allen, Michael H.; Feifel, David; Lesem, Michael D.
- The Journal of Clinical Psychiatry, Vol. 72, Issue 10
The Natural Killer Cell Response to HCV Infection
journal, January 2013
- Ahlenstiel, Golo
- Immune Network, Vol. 13, Issue 5
Works referencing / citing this record:
CD81 as a tumor target
journal, April 2017
- Vences-Catalán, Felipe; Duault, Caroline; Kuo, Chiung-Chi
- Biochemical Society Transactions, Vol. 45, Issue 2
Charting a Path to Success in Virtual Screening
journal, October 2015
- Forli, Stefano
- Molecules, Vol. 20, Issue 10
Peptides derived from CXCL8 based on in silico analysis inhibit CXCL8 interactions with its receptor CXCR1
journal, December 2015
- Jiang, Shinn-Jong; Liou, Je-Wen; Chang, Chun-Chun
- Scientific Reports, Vol. 5, Issue 1
Suramin Inhibits Chikungunya Virus Entry and Transmission
journal, July 2015
- Ho, Yi-Jung; Wang, Yu-Ming; Lu, Jeng-wei
- PLOS ONE, Vol. 10, Issue 7
A Sequence in the loop domain of hepatitis C virus E2 protein identified in silico as crucial for the selective binding to human CD81
journal, May 2017
- Chang, Chun-Chun; Hsu, Hao-Jen; Yen, Jui-Hung
- PLOS ONE, Vol. 12, Issue 5
Charting a Path to Success in Virtual Screening
journal, October 2015
- Forli, Stefano
- Molecules, Vol. 20, Issue 10