The architecture of a eukaryotic replisome
Abstract
At the eukaryotic DNA replication fork, it is widely believed that the Cdc45–Mcm2–7–GINS (CMG) helicase is positioned in front to unwind DNA and that DNA polymerases trail behind the helicase. Here we used single-particle EM to directly image a Saccharomyces cerevisiae replisome. Contrary to expectations, the leading strand Pol ε is positioned ahead of CMG helicase, whereas Ctf4 and the lagging-strand polymerase (Pol) α–primase are behind the helicase. This unexpected architecture indicates that the leading-strand DNA travels a long distance before reaching Pol ε, first threading through the Mcm2–7 ring and then making a U-turn at the bottom and reaching Pol ε at the top of CMG. Lastly, our work reveals an unexpected configuration of the eukaryotic replisome, suggests possible reasons for this architecture and provides a basis for further structural and biochemical replisome studies.
- Authors:
-
- Brookhaven National Lab. (BNL), Upton, NY (United States); Stony Brook Univ., Stony Brook, NY (United States)
- Rockefeller Univ., New York, NY (United States)
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1257963
- Report Number(s):
- BNL-112308-2016-JA
Journal ID: ISSN 1545-9993
- Grant/Contract Number:
- SC00112704
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Structural & Molecular Biology
- Additional Journal Information:
- Journal Volume: 22; Journal Issue: 12; Journal ID: ISSN 1545-9993
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Sun, Jingchuan, Yuan, Zuanning, Shi, Yi, Georgescu, Roxana E., Chait, Brian T., Li, Huilin, and O'Donnell, Michael E. The architecture of a eukaryotic replisome. United States: N. p., 2015.
Web. doi:10.1038/nsmb.3113.
Sun, Jingchuan, Yuan, Zuanning, Shi, Yi, Georgescu, Roxana E., Chait, Brian T., Li, Huilin, & O'Donnell, Michael E. The architecture of a eukaryotic replisome. United States. https://doi.org/10.1038/nsmb.3113
Sun, Jingchuan, Yuan, Zuanning, Shi, Yi, Georgescu, Roxana E., Chait, Brian T., Li, Huilin, and O'Donnell, Michael E. Mon .
"The architecture of a eukaryotic replisome". United States. https://doi.org/10.1038/nsmb.3113. https://www.osti.gov/servlets/purl/1257963.
@article{osti_1257963,
title = {The architecture of a eukaryotic replisome},
author = {Sun, Jingchuan and Yuan, Zuanning and Shi, Yi and Georgescu, Roxana E. and Chait, Brian T. and Li, Huilin and O'Donnell, Michael E.},
abstractNote = {At the eukaryotic DNA replication fork, it is widely believed that the Cdc45–Mcm2–7–GINS (CMG) helicase is positioned in front to unwind DNA and that DNA polymerases trail behind the helicase. Here we used single-particle EM to directly image a Saccharomyces cerevisiae replisome. Contrary to expectations, the leading strand Pol ε is positioned ahead of CMG helicase, whereas Ctf4 and the lagging-strand polymerase (Pol) α–primase are behind the helicase. This unexpected architecture indicates that the leading-strand DNA travels a long distance before reaching Pol ε, first threading through the Mcm2–7 ring and then making a U-turn at the bottom and reaching Pol ε at the top of CMG. Lastly, our work reveals an unexpected configuration of the eukaryotic replisome, suggests possible reasons for this architecture and provides a basis for further structural and biochemical replisome studies.},
doi = {10.1038/nsmb.3113},
journal = {Nature Structural & Molecular Biology},
number = 12,
volume = 22,
place = {United States},
year = {Mon Nov 02 00:00:00 EST 2015},
month = {Mon Nov 02 00:00:00 EST 2015}
}
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Works referenced in this record:
Selective Bypass of a Lagging Strand Roadblock by the Eukaryotic Replicative DNA Helicase
journal, September 2011
- Fu, Yu V.; Yardimci, Hasan; Long, David T.
- Cell, Vol. 146, Issue 6
The structural basis for MCM2–7 helicase activation by GINS and Cdc45
journal, March 2011
- Costa, Alessandro; Ilves, Ivar; Tamberg, Nele
- Nature Structural & Molecular Biology, Vol. 18, Issue 4
Structure of the human GINS complex and its assembly and functional interface in replication initiation
journal, April 2007
- Kamada, Katsuhiko; Kubota, Yumiko; Arata, Toshiaki
- Nature Structural & Molecular Biology, Vol. 14, Issue 5
The Major Roles of DNA Polymerases Epsilon and Delta at the Eukaryotic Replication Fork Are Evolutionarily Conserved
journal, December 2011
- Miyabe, Izumi; Kunkel, Thomas A.; Carr, Antony M.
- PLoS Genetics, Vol. 7, Issue 12
The Histone Fold Subunits of Drosophila CHRAC Facilitate Nucleosome Sliding through Dynamic DNA Interactions
journal, October 2005
- Hartlepp, K. F.; Fernandez-Tornero, C.; Eberharter, A.
- Molecular and Cellular Biology, Vol. 25, Issue 22
Strand-Specific Analysis Shows Protein Binding at Replication Forks and PCNA Unloading from Lagging Strands when Forks Stall
journal, November 2014
- Yu, Chuanhe; Gan, Haiyun; Han, Junhong
- Molecular Cell, Vol. 56, Issue 4
Replicative DNA Polymerases
journal, June 2013
- Johansson, E.; Dixon, N.
- Cold Spring Harbor Perspectives in Biology, Vol. 5, Issue 6
Interaction between human Ctf4 and the Cdc45/Mcm2-7/GINS (CMG) replicative helicase
journal, November 2013
- Kang, Y. -H.; Farina, A.; Bermudez, V. P.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 49
Division of Labor at the Eukaryotic Replication Fork
journal, April 2008
- Nick McElhinny, Stephanie A.; Gordenin, Dmitry A.; Stith, Carrie M.
- Molecular Cell, Vol. 30, Issue 2
Reconsidering DNA Polymerases at the Replication Fork in Eukaryotes
journal, July 2015
- Stillman, Bruce
- Molecular Cell, Vol. 59, Issue 2
Proteomic and genomic characterization of chromatin complexes at a boundary
journal, April 2005
- Tackett, Alan J.; Dilworth, David J.; Davey, Megan J.
- The Journal of Cell Biology, Vol. 169, Issue 1
UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004
- Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
- Journal of Computational Chemistry, Vol. 25, Issue 13
Analysis of the Essential Functions of the C-terminal Protein/Protein Interaction Domain of Saccharomyces cerevisiae pol ε and Its Unexpected Ability to Support Growth in the Absence of the DNA Polymerase Domain
journal, August 1999
- Dua, Rajiv; Levy, Daniel L.; Campbell, Judith L.
- Journal of Biological Chemistry, Vol. 274, Issue 32
DNA polymerase ε and its roles in genome stability: ROLES OF DNA Pol ε IN GENOME STABILITY
journal, May 2014
- Henninger, Erin E.; Pursell, Zachary F.
- IUBMB Life, Vol. 66, Issue 5
EMAN2: An extensible image processing suite for electron microscopy
journal, January 2007
- Tang, Guang; Peng, Liwei; Baldwin, Philip R.
- Journal of Structural Biology, Vol. 157, Issue 1
Isolation of the Cdc45/Mcm2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase
journal, June 2006
- Moyer, S. E.; Lewis, P. W.; Botchan, M. R.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 27
Cryo-EM structure of a helicase loading intermediate containing ORC–Cdc6–Cdt1–MCM2-7 bound to DNA
journal, July 2013
- Sun, Jingchuan; Evrin, Cecile; Samel, Stefan A.
- Nature Structural & Molecular Biology, Vol. 20, Issue 8
A third essential DNA polymerase in S. cerevisiae
journal, September 1990
- Morrison, Alan; Araki, Hiroyuki; Clark, Alan B.
- Cell, Vol. 62, Issue 6
Identification of cross-linked peptides from complex samples
journal, July 2012
- Yang, Bing; Wu, Yan-Jie; Zhu, Ming
- Nature Methods, Vol. 9, Issue 9
Eukaryotic DNA polymerase amino acid sequence required for 3'----5' exonuclease activity.
journal, November 1991
- Morrison, A.; Bell, J. B.; Kunkel, T. A.
- Proceedings of the National Academy of Sciences, Vol. 88, Issue 21
Crystal structure of the GINS complex and functional insights into its role in DNA replication
journal, July 2007
- Chang, Y. P.; Wang, G.; Bermudez, V.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 31
A Major Role of DNA Polymerase δ in Replication of Both the Leading and Lagging DNA Strands
journal, July 2015
- Johnson, Robert E.; Klassen, Roland; Prakash, Louise
- Molecular Cell, Vol. 59, Issue 2
Alternate pathways of DNA replication: DNA polymerase I-dependent replication.
journal, November 1981
- Niwa, O.; Bryan, S. K.; Moses, R. E.
- Proceedings of the National Academy of Sciences, Vol. 78, Issue 11
Strand-Specific Analysis Shows Protein Binding at Replication Forks and PCNA Unloading from Lagging Strands when Forks Stall
journal, November 2014
- Yu, Chuanhe; Gan, Haiyun; Han, Junhong
- Molecular Cell, Vol. 56, Issue 4
3D architecture of DNA Pol α reveals the functional core of multi-subunit replicative polymerases
journal, June 2009
- Klinge, Sebastian; Núñez-Ramírez, Rafael; Llorca, Oscar
- The EMBO Journal, Vol. 28, Issue 13
Noncompetitive Counteractions of DNA Polymerase and ISW2/yCHRAC for Epigenetic Inheritance of Telomere Position Effect in Saccharomyces cerevisiae
journal, December 2003
- Iida, T.; Araki, H.
- Molecular and Cellular Biology, Vol. 24, Issue 1
Structural basis of high-fidelity DNA synthesis by yeast DNA polymerase δ
journal, August 2009
- Swan, Michael K.; Johnson, Robert E.; Prakash, Louise
- Nature Structural & Molecular Biology, Vol. 16, Issue 9
Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit
journal, November 2014
- Cevher, Murat A.; Shi, Yi; Li, Dan
- Nature Structural & Molecular Biology, Vol. 21, Issue 12
The DNA Polymerase Domain of polε Is Required for Rapid, Efficient, and Highly Accurate Chromosomal DNA Replication, Telomere Length Maintenance, and Normal Cell Senescence in Saccharomyces cerevisiae
journal, May 2002
- Ohya, Tomoko; Kawasaki, Yasuo; Hiraga, Shin-Ichiro
- Journal of Biological Chemistry, Vol. 277, Issue 31
Dpb2 Integrates the Leading-Strand DNA Polymerase into the Eukaryotic Replisome
journal, April 2013
- Sengupta, Sugopa; van Deursen, Frederick; de Piccoli, Giacomo
- Current Biology, Vol. 23, Issue 7
Structural basis for processive DNA synthesis by yeast DNA polymerase ε
journal, August 2014
- Hogg, Matthew; Osterman, Pia; Bylund, Göran
- Acta Crystallographica Section A Foundations and Advances, Vol. 70, Issue a1
DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome
journal, August 2014
- Costa, Alessandro; Renault, Ludovic; Swuec, Paolo
- eLife, Vol. 3
Crystal structure of the human GINS complex
journal, June 2007
- Choi, J. M.; Lim, H. S.; Kim, J. J.
- Genes & Development, Vol. 21, Issue 11
Idling by DNA polymerase maintains a ligatable nick during lagging-strand DNA replication
journal, November 2004
- Garg, P.
- Genes & Development, Vol. 18, Issue 22
Dividing the workload at a eukaryotic replication fork
journal, November 2008
- Kunkel, Thomas A.; Burgers, Peter M.
- Trends in Cell Biology, Vol. 18, Issue 11
Structure of Saccharomyces cerevisiae DNA polymerase epsilon by cryo–electron microscopy
journal, December 2005
- Asturias, Francisco J.; Cheung, Iris K.; Sabouri, Nasim
- Nature Structural & Molecular Biology, Vol. 13, Issue 1
Structural and mechanistic insights into Mcm2–7 double-hexamer assembly and function
journal, October 2014
- Sun, Jingchuan; Fernandez-Cid, Alejandra; Riera, Alberto
- Genes & Development, Vol. 28, Issue 20
Mechanism of asymmetric polymerase assembly at the eukaryotic replication fork
journal, July 2014
- Georgescu, Roxana E.; Langston, Lance; Yao, Nina Y.
- Nature Structural & Molecular Biology, Vol. 21, Issue 8
Crystal Structure of Yeast DNA Polymerase ε Catalytic Domain
journal, April 2014
- Jain, Rinku; Rajashankar, Kanagalaghatta R.; Buku, Angeliki
- PLoS ONE, Vol. 9, Issue 4
The Minichromosome Maintenance Replicative Helicase
journal, July 2013
- Bell, S. D.; Botchan, M. R.
- Cold Spring Harbor Perspectives in Biology, Vol. 5, Issue 11
Principles and Concepts of DNA Replication in Bacteria, Archaea, and Eukarya
journal, July 2013
- O'Donnell, M.; Langston, L.; Stillman, B.
- Cold Spring Harbor Perspectives in Biology, Vol. 5, Issue 7
The dna Replication fork in Eukaryotic Cells
journal, June 1998
- Waga, Shou; Stillman, Bruce
- Annual Review of Biochemistry, Vol. 67, Issue 1
CMG helicase and DNA polymerase form a functional 15-subunit holoenzyme for eukaryotic leading-strand DNA replication
journal, October 2014
- Langston, L. D.; Zhang, D.; Yurieva, O.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 43
RELION: Implementation of a Bayesian approach to cryo-EM structure determination
journal, December 2012
- Scheres, Sjors H. W.
- Journal of Structural Biology, Vol. 180, Issue 3, p. 519-530
Yeast DNA Polymerase Participates in Leading-Strand DNA Replication
journal, July 2007
- Pursell, Z. F.; Isoz, I.; Lundstrom, E. -B.
- Science, Vol. 317, Issue 5834
Structural Characterization by Cross-linking Reveals the Detailed Architecture of a Coatomer-related Heptameric Module from the Nuclear Pore Complex
journal, August 2014
- Shi, Yi; Fernandez-Martinez, Javier; Tjioe, Elina
- Molecular & Cellular Proteomics, Vol. 13, Issue 11
Preclinical Development of a Fusion Peptide Conjugate as an HIV Vaccine Immunogen
journal, February 2020
- Ou, Li; Kong, Wing-Pui; Chuang, Gwo-Yu
- Scientific Reports, Vol. 10, Issue 1
Activation of the MCM2-7 Helicase by Association with Cdc45 and GINS Proteins
journal, January 2010
- Ilves, Ivar; Petojevic, Tatjana; Pesavento, James J.
- Molecular Cell, Vol. 37, Issue 2
Bypass of a protein barrier by a replicative DNA helicase
journal, November 2012
- Yardimci, Hasan; Wang, Xindan; Loveland, Anna B.
- Nature, Vol. 492, Issue 7428
A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome
journal, May 2014
- Simon, Aline C.; Zhou, Jin C.; Perera, Rajika L.
- Nature, Vol. 510, Issue 7504
Structure of the eukaryotic MCM complex at 3.8 Å
journal, July 2015
- Li, Ningning; Zhai, Yuanliang; Zhang, Yixiao
- Nature, Vol. 524, Issue 7564
Tracking replication enzymology in vivo by genome-wide mapping of ribonucleotide incorporation
journal, January 2015
- Clausen, Anders R.; Lujan, Scott A.; Burkholder, Adam B.
- Nature Structural & Molecular Biology, Vol. 22, Issue 3
Structural basis for processive DNA synthesis by yeast DNA polymerase ɛ
journal, December 2013
- Hogg, Matthew; Osterman, Pia; Bylund, Göran O.
- Nature Structural & Molecular Biology, Vol. 21, Issue 1
Polymerase Dynamics at the Eukaryotic DNA Replication Fork
journal, October 2008
- Burgers, Peter M. J.
- Journal of Biological Chemistry, Vol. 284, Issue 7
Cdc45 (cell division cycle protein 45) guards the gate of the Eukaryote Replisome helicase stabilizing leading strand engagement
journal, January 2015
- Petojevic, Tatjana; Pesavento, James J.; Costa, Alessandro
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 3
3′ → 5′ Exonucleases of DNA Polymerases ϵ and δ Correct Base Analog Induced DNA Replication Errors on Opposite DNA Strands in Saccharomyces cerevisiae
journal, March 1996
- Shcherbakova, Polina V.; Pavlov, Youri I.
- Genetics, Vol. 142, Issue 3
Works referencing / citing this record:
DNA replication protein Cdc45 directly interacts with PCNA via its PIP box in Leishmania donovani and the Cdc45 PIP box is essential for cell survival
posted_content, November 2019
- Yadav, Aarti; Sharma, Varshni; Pal, Jyoti
- bioRxiv
The eukaryotic CMG helicase pumpjack and integration into the replisome
journal, March 2016
- Sun, Jingchuan; Yuan, Zuanning; Georgescu, Roxanna
- Nucleus, Vol. 7, Issue 2
The Human Replicative Helicase, the CMG Complex, as a Target for Anti-cancer Therapy
journal, March 2018
- Seo, Yeon-Soo; Kang, Young-Hoon
- Frontiers in Molecular Biosciences, Vol. 5
Ctf4 organizes sister replisomes and Pol α into a replication factory
preprint, August 2019
- Yuan, Zuanning; Georgescu, Roxana; de Luna Almeida Santos, Ruda
The Eukaryotic CMG Helicase at the Replication Fork: Emerging Architecture Reveals an Unexpected Mechanism
journal, February 2018
- Li, Huilin; O'Donnell, Michael E.
- BioEssays, Vol. 40, Issue 3
Nuclear Pore Complexes: Global Conservation and Local Variation
journal, June 2018
- Holzer, Guillaume; Antonin, Wolfram
- Current Biology, Vol. 28, Issue 11
Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase
journal, February 2017
- Lõoke, Marko; Maloney, Michael F.; Bell, Stephen P.
- Genes & Development, Vol. 31, Issue 3
Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork
journal, June 2020
- Baretić, Domagoj; Jenkyn-Bedford, Michael; Aria, Valentina
- Molecular Cell, Vol. 78, Issue 5
Tunability of DNA polymerase stability during eukaryotic DNA replication
posted_content, June 2019
- Lewis, Jacob S.; Spenkelink, Lisanne M.; Schauer, Grant D.
- Molecular Cell
The Human Replicative Helicase, the CMG Complex, as a Target for Anti-cancer Therapy
journal, March 2018
- Seo, Yeon-Soo; Kang, Young-Hoon
- Frontiers in Molecular Biosciences, Vol. 5
POLE3-POLE4 Is a Histone H3-H4 Chaperone that Maintains Chromatin Integrity during DNA Replication
journal, October 2018
- Bellelli, Roberto; Belan, Ondrej; Pye, Valerie E.
- Molecular Cell, Vol. 72, Issue 1
DNA unwinding mechanism of a eukaryotic replicative CMG helicase
journal, February 2020
- Yuan, Zuanning; Georgescu, Roxana; Bai, Lin
- Nature Communications, Vol. 11, Issue 1
From structure to mechanism—understanding initiation of DNA replication
journal, June 2017
- Riera, Alberto; Barbon, Marta; Noguchi, Yasunori
- Genes & Development, Vol. 31, Issue 11
Cryo-EM structures of the eukaryotic replicative helicase bound to a translocation substrate
journal, February 2016
- Abid Ali, Ferdos; Renault, Ludovic; Gannon, Julian
- Nature Communications, Vol. 7, Issue 1
Initiation-specific alleles of the Cdc45 helicase-activating protein
journal, March 2019
- Rios-Morales, Ramon Y.; Chan, Sze Ham; Bell, Stephen P.
- PLOS ONE, Vol. 14, Issue 3
Integrative Structure and Functional Anatomy of a Nuclear Pore Complex
journal, August 2018
- Nudelman, I.; Kim, S. J.; Fernandez-Martinez, J.
- Microscopy and Microanalysis, Vol. 24, Issue S1
Dynamic structural insights into the molecular mechanism of DNA unwinding by the bacteriophage T7 helicase
journal, February 2020
- Ma, Jian-Bing; Chen, Ze; Xu, Chun-Hua
- Nucleic Acids Research, Vol. 48, Issue 6
MCMs in Cancer: Prognostic Potential and Mechanisms
journal, February 2020
- Yu, Si; Wang, Guanqun; Shi, Yue
- Analytical Cellular Pathology, Vol. 2020
Tunability of DNA Polymerase Stability during Eukaryotic DNA Replication
journal, January 2020
- Lewis, Jacob S.; Spenkelink, Lisanne M.; Schauer, Grant D.
- Molecular Cell, Vol. 77, Issue 1
The mechanism of eukaryotic CMG helicase activation
journal, February 2018
- Douglas, Max E.; Ali, Ferdos Abid; Costa, Alessandro
- Nature, Vol. 555, Issue 7695
DNA replication through a chromatin environment
journal, August 2017
- Bellush, James M.; Whitehouse, Iestyn
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 372, Issue 1731
Acute hydroxyurea-induced replication blockade results in replisome components disengagement from nascent DNA without causing fork collapse
journal, July 2019
- Ercilla, Amaia; Feu, Sonia; Aranda, Sergi
- Cellular and Molecular Life Sciences
Cryo-EM structures of the eukaryotic replicative helicase bound to a translocation substrate
text, January 2016
- Ali, Ferdos A.; Renault, Ludovic; Gannon, Julian
- ETH Zurich
Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation
journal, January 2017
- Georgescu, Roxana; Yuan, Zuanning; Bai, Lin
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 5
Eukaryotic Replicative Helicase Subunit Interaction with DNA and Its Role in DNA Replication
journal, April 2017
- Martinez, Matthew P.; Wacker, Amanda L.; Bruck, Irina
- Genes, Vol. 8, Issue 4
The human papillomavirus DNA helicase E1 binds, stimulates, and confers processivity to cellular DNA polymerase epsilon
journal, November 2017
- Chojnacki, Michaelle; Melendy, Thomas
- Nucleic Acids Research, Vol. 46, Issue 1
Initiation-specific alleles of the Cdc45 helicase-activating protein
journal, March 2019
- Rios-Morales, Ramon Y.; Chan, Sze Ham; Bell, Stephen P.
- PLOS ONE, Vol. 14, Issue 3
Molecular basis for ATP-hydrolysis-driven DNA translocation by the CMG helicase of the eukaryotic replisome.
text, January 2020
- Eickhoff, Patrik; Kose, Hazal B.; Martino, Fabrizio
- The Francis Crick Institute
Elaborated Action of the Human Primosome
journal, February 2017
- Baranovskiy, Andrey; Tahirov, Tahir
- Genes, Vol. 8, Issue 2
DNA looping mediates nucleosome transfer
journal, November 2016
- Brennan, Lucy D.; Forties, Robert A.; Patel, Smita S.
- Nature Communications, Vol. 7, Issue 1
When proteins play tag: the dynamic nature of the replisome
journal, July 2019
- Mueller, Stefan H.; Spenkelink, Lisanne M.; van Oijen, Antoine M.
- Biophysical Reviews, Vol. 11, Issue 4
Chromosome Duplication in Saccharomyces cerevisiae
journal, July 2016
- Bell, Stephen P.; Labib, Karim
- Genetics, Vol. 203, Issue 3
Integrative structure and functional anatomy of a nuclear pore complex
journal, March 2018
- Kim, Seung Joong; Fernandez-Martinez, Javier; Nudelman, Ilona
- Nature, Vol. 555, Issue 7697
The ring-shaped hexameric helicases that function at DNA replication forks
journal, January 2018
- O’Donnell, Michael E.; Li, Huilin
- Nature Structural & Molecular Biology, Vol. 25, Issue 2
Fundamental Characteristics of AAA+ Protein Family Structure and Function
journal, January 2016
- Miller, Justin M.; Enemark, Eric J.
- Archaea, Vol. 2016
Origin DNA Melting—An Essential Process with Divergent Mechanisms
journal, January 2017
- Martinez, Matthew; Jones, John; Bruck, Irina
- Genes, Vol. 8, Issue 1
Emerging Roles for Ciz1 in Cell Cycle Regulation and as a Driver of Tumorigenesis
journal, December 2016
- Pauzaite, Tekle; Thacker, Urvi; Tollitt, James
- Biomolecules, Vol. 7, Issue 4
Arranging eukaryotic nuclear DNA polymerases for replication: Specific interactions with accessory proteins arrange Pols α, δ, and ϵ in the replisome for leading-strand and lagging-strand DNA replication
journal, July 2017
- Kunkel, Thomas A.; Burgers, Peter M. J.
- BioEssays, Vol. 39, Issue 8
Possible function of the second RecJ-like protein in stalled replication fork repair by interacting with Hef
journal, December 2017
- Nagata, Mariko; Ishino, Sonoko; Yamagami, Takeshi
- Scientific Reports, Vol. 7, Issue 1
The Cdc45/RecJ-like protein forms a complex with GINS and MCM, and is important for DNA replication in Thermococcus kodakarensis
journal, August 2017
- Nagata, Mariko; Ishino, Sonoko; Yamagami, Takeshi
- Nucleic Acids Research, Vol. 45, Issue 18
Action of CMG with strand-specific DNA blocks supports an internal unwinding mode for the eukaryotic replicative helicase
journal, March 2017
- Langston, Lance; O’Donnell, Mike
- eLife, Vol. 6
Centromere Stability: The Replication Connection
journal, January 2017
- Forsburg, Susan; Shen, Kuo-Fang
- Genes, Vol. 8, Issue 1
Structural Mechanisms of Hexameric Helicase Loading, Assembly, and Unwinding
journal, January 2016
- Trakselis, Michael A.
- F1000Research, Vol. 5
The absence of the catalytic domains of Saccharomyces cerevisiae DNA polymerase ϵ strongly reduces DNA replication fidelity
journal, January 2019
- Garbacz, Marta A.; Cox, Phillip B.; Sharma, Sushma
- Nucleic Acids Research, Vol. 47, Issue 8
Architecture of Pol II(G) and molecular mechanism of transcription regulation by Gdown1
journal, September 2018
- Jishage, Miki; Yu, Xiaodi; Shi, Yi
- Nature Structural & Molecular Biology, Vol. 25, Issue 9
Replisome‐Cohesin Interfacing: A Molecular Perspective
journal, August 2018
- Villa‐Hernández, Sara; Bermejo, Rodrigo
- BioEssays, Vol. 40, Issue 10
Crystal structure of the human Polϵ B-subunit in complex with the C-terminal domain of the catalytic subunit
journal, July 2017
- Baranovskiy, Andrey G.; Gu, Jianyou; Babayeva, Nigar D.
- Journal of Biological Chemistry, Vol. 292, Issue 38
Structures and operating principles of the replisome
journal, January 2019
- Gao, Yang; Cui, Yanxiang; Fox, Tara
- Science, Vol. 363, Issue 6429
Acute Hydroxyurea-Induced Replication Blockade Results in Replisome Components Disengagement from Nascent DNA Without Causing Fork Collapse
journal, January 2018
- Ercilla, Amaia; Feu, Sonia; Aranda, Sergi
- SSRN Electronic Journal
Cortical Development and Brain Malformations: Insights From the Differential Regulation of Early Events of DNA Replication
journal, March 2019
- Kalogeropoulou, Argyro; Lygerou, Zoi; Taraviras, Stavros
- Frontiers in Cell and Developmental Biology, Vol. 7
Characterization of the dimeric CMG/pre-initiation complex and its transition into DNA replication forks
journal, November 2019
- Liu, Lu; Zhang, Yue; Zhang, Jingjing
- Cellular and Molecular Life Sciences, Vol. 77, Issue 15
Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome
journal, November 2018
- Goswami, Panchali; Abid Ali, Ferdos; Douglas, Max E.
- Nature Communications, Vol. 9, Issue 1
Structural Mechanisms of Hexameric Helicase Loading, Assembly, and Unwinding
journal, January 2016
- Trakselis, Michael A.
- F1000Research, Vol. 5
Histone chaperone networks shaping chromatin function
journal, January 2017
- Hammond, Colin M.; Strømme, Caroline B.; Huang, Hongda
- Nature Reviews Molecular Cell Biology, Vol. 18, Issue 3
Quality control mechanisms exclude incorrect polymerases from the eukaryotic replication fork
journal, January 2017
- Schauer, Grant D.; O’Donnell, Michael E.
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 4
Cryo-EM structure of the replisome reveals multiple interactions coordinating DNA synthesis
journal, February 2017
- Kulczyk, Arkadiusz W.; Moeller, Arne; Meyer, Peter
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 10
DNA replication protein Cdc45 directly interacts with PCNA via its PIP box in Leishmania donovani and the Cdc45 PIP box is essential for cell survival
journal, May 2020
- Yadav, Aarti; Sharma, Varshni; Pal, Jyoti
- PLOS Pathogens, Vol. 16, Issue 5
The eukaryotic CMG helicase pumpjack and integration into the replisome
journal, March 2016
- Sun, Jingchuan; Yuan, Zuanning; Georgescu, Roxanna
- Nucleus, Vol. 7, Issue 2
Recent advances in cohesin biology
journal, August 2016
- Rankin, Susannah; Dawson, Dean S.
- F1000Research, Vol. 5
Reconstitution of a eukaryotic replisome reveals the mechanism of asymmetric distribution of DNA polymerases
journal, July 2016
- Yurieva, Olga; O'Donnell, Mike
- Nucleus, Vol. 7, Issue 4
CMG–Pol epsilon dynamics suggests a mechanism for the establishment of leading-strand synthesis in the eukaryotic replisome
journal, April 2017
- Zhou, Jin Chuan; Janska, Agnieszka; Goswami, Panchali
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 16