Forming giant-sized polymersomes using gel-assisted rehydration
Abstract
Here, we present a protocol to rapidly form giant polymer vesicles (pGVs). Briefly, polymer solutions are dehydrated on dried agarose films adhered to coverslips. Rehydration of the polymer films results in rapid formation of pGVs. This method greatly advances the preparation of synthetic giant vesicles for direct applications in biomimetic studies.
- Authors:
-
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1257838
- Report Number(s):
- SAND-2016-2307J
Journal ID: ISSN 1940-087X; jove; 621961
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Visualized Experiments
- Additional Journal Information:
- Journal Issue: 111; Journal ID: ISSN 1940-087X
- Publisher:
- MyJoVE Corp.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; polymersome; gel; vesicle; rehydration; polymer; membrane
Citation Formats
Greene, Adrienne C., Sasaki, Darryl Y., and Bachand, George D. Forming giant-sized polymersomes using gel-assisted rehydration. United States: N. p., 2016.
Web. doi:10.3791/54051.
Greene, Adrienne C., Sasaki, Darryl Y., & Bachand, George D. Forming giant-sized polymersomes using gel-assisted rehydration. United States. https://doi.org/10.3791/54051
Greene, Adrienne C., Sasaki, Darryl Y., and Bachand, George D. Thu .
"Forming giant-sized polymersomes using gel-assisted rehydration". United States. https://doi.org/10.3791/54051. https://www.osti.gov/servlets/purl/1257838.
@article{osti_1257838,
title = {Forming giant-sized polymersomes using gel-assisted rehydration},
author = {Greene, Adrienne C. and Sasaki, Darryl Y. and Bachand, George D.},
abstractNote = {Here, we present a protocol to rapidly form giant polymer vesicles (pGVs). Briefly, polymer solutions are dehydrated on dried agarose films adhered to coverslips. Rehydration of the polymer films results in rapid formation of pGVs. This method greatly advances the preparation of synthetic giant vesicles for direct applications in biomimetic studies.},
doi = {10.3791/54051},
journal = {Journal of Visualized Experiments},
number = 111,
volume = ,
place = {United States},
year = {Thu May 26 00:00:00 EDT 2016},
month = {Thu May 26 00:00:00 EDT 2016}
}
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