Structural characterization of a mixed-linkage glucan deficient mutant reveals alteration in cellulose microfibril orientation in rice coleoptile mesophyll cell walls
Abstract
The CELLULOSE SYNTHASE-LIKE F6 (CslF6) gene was previously shown to mediate the biosynthesis of mixed-linkage glucan (MLG), a cell wall polysaccharide that is hypothesized to be tightly associated with cellulose and also have a role in cell expansion in the primary cell wall of young seedlings in grass species. We have recently shown that loss-of-function cslf6 rice mutants do not accumulate MLG in most vegetative tissues. Despite the absence of a structurally important polymer, MLG, these mutants are unexpectedly viable and only show a moderate growth compromise compared to wild type. Therefore these mutants are ideal biological systems to test the current grass cell wall model. In order to gain a better understanding of the role of MLG in the primary wall, we performed in-depth compositional and structural analyses of the cell walls of 3 day-old rice seedlings using various biochemical and novel microspectroscopic approaches. We found that cellulose content as well as matrix polysaccharide composition was not significantly altered in the MLG deficient mutant. However, we observed a significant change in cellulose microfibril bundle organization in mesophyll cell walls of the cslf6 mutant. Using synchrotron source Fourier Transform Mid-Infrared (FTM-IR) Spectromicroscopy for high-resolution imaging, we determined that the bondsmore »
- Authors:
-
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Univ. of Copenhagen, Copenhagen (Denmark)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Davis, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1257262
- Alternate Identifier(s):
- OSTI ID: 1512230
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Volume: 6; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; type II cell walls; cellulose; FT-MIR spectroscopy; mixed-linkage glucan; primary cell wall; rice
Citation Formats
Smith-Moritz, Andreia M., Hao, Zhao, Fernández-Nino, Susana G., Fangel, Jonatan U., Verhertbruggen, Yves, Holman, Hoi-Ying N., Willats, William G. T., Ronald, Pamela C., Scheller, Henrik V., Heazlewood, Joshua L., and Vega-Sanchez, Miguel E. Structural characterization of a mixed-linkage glucan deficient mutant reveals alteration in cellulose microfibril orientation in rice coleoptile mesophyll cell walls. United States: N. p., 2015.
Web. doi:10.3389/fpls.2015.00628.
Smith-Moritz, Andreia M., Hao, Zhao, Fernández-Nino, Susana G., Fangel, Jonatan U., Verhertbruggen, Yves, Holman, Hoi-Ying N., Willats, William G. T., Ronald, Pamela C., Scheller, Henrik V., Heazlewood, Joshua L., & Vega-Sanchez, Miguel E. Structural characterization of a mixed-linkage glucan deficient mutant reveals alteration in cellulose microfibril orientation in rice coleoptile mesophyll cell walls. United States. https://doi.org/10.3389/fpls.2015.00628
Smith-Moritz, Andreia M., Hao, Zhao, Fernández-Nino, Susana G., Fangel, Jonatan U., Verhertbruggen, Yves, Holman, Hoi-Ying N., Willats, William G. T., Ronald, Pamela C., Scheller, Henrik V., Heazlewood, Joshua L., and Vega-Sanchez, Miguel E. Tue .
"Structural characterization of a mixed-linkage glucan deficient mutant reveals alteration in cellulose microfibril orientation in rice coleoptile mesophyll cell walls". United States. https://doi.org/10.3389/fpls.2015.00628. https://www.osti.gov/servlets/purl/1257262.
@article{osti_1257262,
title = {Structural characterization of a mixed-linkage glucan deficient mutant reveals alteration in cellulose microfibril orientation in rice coleoptile mesophyll cell walls},
author = {Smith-Moritz, Andreia M. and Hao, Zhao and Fernández-Nino, Susana G. and Fangel, Jonatan U. and Verhertbruggen, Yves and Holman, Hoi-Ying N. and Willats, William G. T. and Ronald, Pamela C. and Scheller, Henrik V. and Heazlewood, Joshua L. and Vega-Sanchez, Miguel E.},
abstractNote = {The CELLULOSE SYNTHASE-LIKE F6 (CslF6) gene was previously shown to mediate the biosynthesis of mixed-linkage glucan (MLG), a cell wall polysaccharide that is hypothesized to be tightly associated with cellulose and also have a role in cell expansion in the primary cell wall of young seedlings in grass species. We have recently shown that loss-of-function cslf6 rice mutants do not accumulate MLG in most vegetative tissues. Despite the absence of a structurally important polymer, MLG, these mutants are unexpectedly viable and only show a moderate growth compromise compared to wild type. Therefore these mutants are ideal biological systems to test the current grass cell wall model. In order to gain a better understanding of the role of MLG in the primary wall, we performed in-depth compositional and structural analyses of the cell walls of 3 day-old rice seedlings using various biochemical and novel microspectroscopic approaches. We found that cellulose content as well as matrix polysaccharide composition was not significantly altered in the MLG deficient mutant. However, we observed a significant change in cellulose microfibril bundle organization in mesophyll cell walls of the cslf6 mutant. Using synchrotron source Fourier Transform Mid-Infrared (FTM-IR) Spectromicroscopy for high-resolution imaging, we determined that the bonds associated with cellulose and arabinoxylan, another major component of the primary cell walls of grasses, were in a lower energy configuration compared to wild type, suggesting a slightly weaker primary wall in MLG deficient mesophyll cells. Taken together, these results suggest that MLG may influence cellulose deposition in mesophyll cell walls without significantly affecting anisotropic growth thus challenging MLG importance in cell wall expansion.},
doi = {10.3389/fpls.2015.00628},
journal = {Frontiers in Plant Science},
number = ,
volume = 6,
place = {United States},
year = {Tue Aug 18 00:00:00 EDT 2015},
month = {Tue Aug 18 00:00:00 EDT 2015}
}
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Figures / Tables:
FIGURE 1: Comprehensive microarray polymer profiling (ComPP) of wild type (NPB) and mutant lines cslf6-1 and cslf6-2. Rice cell wall material was sequentially extracted with CDTA followed by NaOH. The extracts were then subsequently printed on nitrocellulose membranes and probed with a library of cell wall specific monoclonal antibodies tomore »
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