High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris
Abstract
We report that Ensifer meliloti 4H41 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of common bean (Phaseolus vulgaris). Strain 4H41 was isolated in 2002 from root nodules of P. vulgaris grown in South Tunisia from the oasis of Rjim-Maatoug. Strain 4H41 is salt- and drought-tolerant and highly effective at fixing nitrogen with P. vulgaris. Here we describe the features of E. meliloti 4H41, together with genome sequence information and its annotation. The 6,795,637 bp high-quality permanent draft genome is arranged into 47 scaffolds of 47 contigs containing 6,350 protein-coding genes and 72 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.
- Authors:
-
- Centre of Biotechnology of Borj-Cedria, Hammam-Lif (Tunisia)
- Murdoch Univ., Western Australia (Australia)
- DOE Joint Genome Inst., Walnut Creek, CA (United States)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- DOE Joint Genome Inst., Walnut Creek, CA (United States); King Abdulaziz Univ., Jeddah (Saudi Arabia)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1256908
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Standards in Genomic Sciences
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1944-3277
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Root-nodule bacteria; Nitrogen fixation; Alphaproteobacteria; Ensifer; Phaseolus vulgaris
Citation Formats
Mhamdi, Ridha, Ardley, Julie, Tian, Rui, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Markowitz, Victor, Ivanova, Natalia, Kyrpides, Nikos, and Reeve, Wayne. High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris. United States: N. p., 2015.
Web. doi:10.1186/s40793-015-0005-1.
Mhamdi, Ridha, Ardley, Julie, Tian, Rui, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Markowitz, Victor, Ivanova, Natalia, Kyrpides, Nikos, & Reeve, Wayne. High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris. United States. https://doi.org/10.1186/s40793-015-0005-1
Mhamdi, Ridha, Ardley, Julie, Tian, Rui, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Markowitz, Victor, Ivanova, Natalia, Kyrpides, Nikos, and Reeve, Wayne. Thu .
"High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris". United States. https://doi.org/10.1186/s40793-015-0005-1. https://www.osti.gov/servlets/purl/1256908.
@article{osti_1256908,
title = {High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris},
author = {Mhamdi, Ridha and Ardley, Julie and Tian, Rui and Seshadri, Rekha and Reddy, T. B. K. and Pati, Amrita and Woyke, Tanja and Markowitz, Victor and Ivanova, Natalia and Kyrpides, Nikos and Reeve, Wayne},
abstractNote = {We report that Ensifer meliloti 4H41 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of common bean (Phaseolus vulgaris). Strain 4H41 was isolated in 2002 from root nodules of P. vulgaris grown in South Tunisia from the oasis of Rjim-Maatoug. Strain 4H41 is salt- and drought-tolerant and highly effective at fixing nitrogen with P. vulgaris. Here we describe the features of E. meliloti 4H41, together with genome sequence information and its annotation. The 6,795,637 bp high-quality permanent draft genome is arranged into 47 scaffolds of 47 contigs containing 6,350 protein-coding genes and 72 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.},
doi = {10.1186/s40793-015-0005-1},
journal = {Standards in Genomic Sciences},
number = 1,
volume = 10,
place = {United States},
year = {Thu Jul 02 00:00:00 EDT 2015},
month = {Thu Jul 02 00:00:00 EDT 2015}
}
Web of Science
Works referenced in this record:
The DOE-JGI Standard Operating Procedure for the Annotations of Microbial Genomes
journal, July 2009
- Mavromatis, Konstantinos; Ivanova, Natalia N.; Chen, I-Min A.
- Standards in Genomic Sciences, Vol. 1, Issue 1
Salt-tolerant rhizobia isolated from a Tunisian oasis that are highly effective for symbiotic N2-fixation with Phaseolus vulgaris constitute a novel biovar (bv. mediterranense) of Sinorhizobium meliloti
journal, September 2006
- Mnasri, Bacem; Mrabet, Moncef; Laguerre, Gisèle
- Archives of Microbiology, Vol. 187, Issue 1
Genetic diversity of Amomum xanthioides and its related species from Southeast Asia and China
journal, May 2021
- Sone, Mikako; Zhu, Shu; Cheng, Xiao
- Journal of Natural Medicines, Vol. 75, Issue 4
Genome-wide identification, structural analysis and expression profiles of GRAS gene family in orchardgrass
journal, February 2020
- Xu, Xiaoheng; Feng, Guangyan; Huang, Linkai
- Molecular Biology Reports, Vol. 47, Issue 3
Advances in Rhizobium Research
journal, July 2002
- Sessitsch, A.; Howieson, J. G.; Perret, X.
- Critical Reviews in Plant Sciences, Vol. 21, Issue 4
Rhizobia from Lanzarote, the Canary Islands, That Nodulate
Phaseolus vulgaris
Have Characteristics in Common with
Sinorhizobium meliloti
Isolates from Mainland Spain
journal, April 2009
- Zurdo-Piñeiro, José Luis; García-Fraile, Paula; Rivas, Raúl
- Applied and Environmental Microbiology, Vol. 75, Issue 8
Characterization of root-nodulating bacteria associated to Prosopis farcta growing in the arid regions of Tunisia
journal, February 2011
- Fterich, A.; Mahdhi, M.; Caviedes, M. A.
- Archives of Microbiology, Vol. 193, Issue 6
SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB
journal, November 2007
- Pruesse, E.; Quast, C.; Knittel, K.
- Nucleic Acids Research, Vol. 35, Issue 21
Velvet: Algorithms for de novo short read assembly using de Bruijn graphs
journal, February 2008
- Zerbino, D. R.; Birney, E.
- Genome Research, Vol. 18, Issue 5
A Genomic Encyclopedia of the Root Nodule Bacteria: assessing genetic diversity through a systematic biogeographic survey
journal, February 2015
- Reeve, Wayne; Ardley, Julie; Tian, Rui
- Standards in Genomic Sciences, Vol. 10, Issue 1
Multilocus sequence analysis of Brazilian Rhizobium microsymbionts of common bean (Phaseolus vulgaris L.) reveals unexpected taxonomic diversity
journal, May 2009
- Ribeiro, Renan Augusto; Barcellos, Fernando Gomes; Thompson, Fabiano L.
- Research in Microbiology, Vol. 160, Issue 4
Dietary palmitic acid promotes a prometastatic memory via Schwann cells
journal, November 2021
- Pascual, Gloria; Domínguez, Diana; Elosúa-Bayes, Marc
- Nature, Vol. 599, Issue 7885
Nodulation and growth of common bean (Phaseolus vulgaris) under water deficiency
journal, July 2007
- Mnasri, Bacem; Aouani, Mohamed Elarbi; Mhamdi, Ridha
- Soil Biology and Biochemistry, Vol. 39, Issue 7
tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence
journal, March 1997
- Lowe, Todd M.; Eddy, Sean R.
- Nucleic Acids Research, Vol. 25, Issue 5
Prodigal: prokaryotic gene recognition and translation initiation site identification
journal, March 2010
- Hyatt, Doug; Chen, Gwo-Liang; LoCascio, Philip F.
- BMC Bioinformatics, Vol. 11, Issue 1
Polyphasic Taxonomy of Rhizobia: Emendation of the Genus Sinorhizobium and Description of Sinorhizobium meliloti comb. nov., Sinorhizobium saheli sp. nov., and Sinorhizobium teranga sp. nov.
journal, October 1994
- De Lajudie, P.; Willems, A.; Pot, B.
- International Journal of Systematic Bacteriology, Vol. 44, Issue 4
Potential for inoculation of common bean by effective rhizobia in Tunisian soils
journal, January 1997
- Aouani, M. E.; Mhamdi, R.; Mars, M.
- Agronomie, Vol. 17, Issue 9-10
Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.
journal, August 2009
- Merabet, C.; Martens, M.; Mahdhi, M.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 60, Issue 3
Symbiotic diversity of Ensifer meliloti strains recovered from various legume species in Tunisia
journal, December 2009
- Mnasri, Bacem; Badri, Yazid; Saïdi, Sabrine
- Systematic and Applied Microbiology, Vol. 32, Issue 8
The Fast Changing Landscape of Sequencing Technologies and Their Impact on Microbial Genome Assemblies and Annotation
journal, December 2012
- Mavromatis, Konstantinos; Land, Miriam L.; Brettin, Thomas S.
- PLoS ONE, Vol. 7, Issue 12
Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.
journal, June 1990
- Woese, C. R.; Kandler, O.; Wheelis, M. L.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 12
Influence of the Size of Indigenous Rhizobial Populations on Establishment and Symbiotic Performance of Introduced Rhizobia on Field-Grown Legumes
journal, January 1991
- Thies, Janice E.; Singleton, Paul W.; Bohlool, B. Ben
- Applied and Environmental Microbiology, Vol. 57, Issue 1
IMG ER: a system for microbial genome annotation expert review and curation
journal, June 2009
- Markowitz, Victor M.; Mavromatis, Konstantinos; Ivanova, Natalia N.
- Bioinformatics, Vol. 25, Issue 17
Phylogenetic position and revised classification ofAcacia s.l.(Fabaceae: Mimosoideae) in Africa, including new combinations inVachelliaandSenegalia
journal, May 2013
- Kyalangalilwa, Bruce; Boatwright, James S.; Daru, Barnabas H.
- Botanical Journal of the Linnean Society, Vol. 172, Issue 4
Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species
journal, November 2011
- Kim, O. -S.; Cho, Y. -J.; Lee, K.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 62, Issue Pt 3
The Genomes OnLine Database (GOLD) v.5: a metadata management system based on a four level (meta)genome project classification
journal, October 2014
- Reddy, T. B. K.; Thomas, Alex D.; Stamatis, Dimitri
- Nucleic Acids Research, Vol. 43, Issue D1
Genotypic diversity and symbiotic effectiveness of rhizobia isolated from root nodules of Phaseolus vulgaris L. grown in Tunisian soils
journal, January 1999
- Mhamdi, R.; Jebara, M.; Aouani, M. E.
- Biology and Fertility of Soils, Vol. 28, Issue 3
Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.)
journal, January 2012
- Ormeño-Orrillo, Ernesto; Menna, Pâmela; Almeida, Luiz Gonzaga P.
- BMC Genomics, Vol. 13, Issue 1
High-quality draft assemblies of mammalian genomes from massively parallel sequence data
journal, December 2010
- Gnerre, S.; MacCallum, I.; Przybylski, D.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 4
Sinorhizobium americanum symbiovar mediterranense is a predominant symbiont that nodulates and fixes nitrogen with common bean (Phaseolus vulgaris L.) in a Northern Tunisian field
journal, June 2012
- Mnasri, Bacem; Saïdi, Sabrine; Chihaoui, Saif-Allah
- Systematic and Applied Microbiology, Vol. 35, Issue 4
PICRUSt2 for prediction of metagenome functions
journal, June 2020
- Douglas, Gavin M.; Maffei, Vincent J.; Zaneveld, Jesse R.
- Nature Biotechnology, Vol. 38, Issue 6
Multilocus sequence analysis of Brazilian Rhizobium microsymbionts of common bean (Phaseolus vulgaris L.) reveals unexpected taxonomic diversity
journal, May 2009
- Ribeiro, Renan Augusto; Barcellos, Fernando Gomes; Thompson, Fabiano L.
- Research in Microbiology, Vol. 160, Issue 4
Methods for enhancing symbiotic nitrogen fixation
journal, January 1993
- Hardarson, Gudni
- Plant and Soil, Vol. 152, Issue 1
Promiscuous nodulation of Phaseolus vulgaris, Macroptilium atropurpureum, and Leucaena leucocephala by indigenous Rhizobium meliloti
journal, May 1990
- Bromfield, E. S. P.; Barran, L. R.
- Canadian Journal of Microbiology, Vol. 36, Issue 5
Potential for inoculation of common bean by effective rhizobia in Tunisian soils
journal, January 1997
- Aouani, M. E.; Mhamdi, R.; Mars, M.
- Agronomie, Vol. 17, Issue 9-10
Genotypic diversity and symbiotic effectiveness of rhizobia isolated from root nodules of Phaseolus vulgaris L. grown in Tunisian soils
journal, January 1999
- Mhamdi, R.; Jebara, M.; Aouani, M. E.
- Biology and Fertility of Soils, Vol. 28, Issue 3
Influence of the Size of Indigenous Rhizobial Populations on Establishment and Symbiotic Performance of Introduced Rhizobia on Field-Grown Legumes
journal, January 1991
- Thies, Janice E.; Singleton, Paul W.; Bohlool, B. Ben
- Applied and Environmental Microbiology, Vol. 57, Issue 1
Nodulation and growth of common bean (Phaseolus vulgaris) under water deficiency
journal, July 2007
- Mnasri, Bacem; Aouani, Mohamed Elarbi; Mhamdi, Ridha
- Soil Biology and Biochemistry, Vol. 39, Issue 7
Advances in Rhizobium Research
journal, July 2002
- Sessitsch, A.; Howieson, J. G.; Perret, X.
- Critical Reviews in Plant Sciences, Vol. 21, Issue 4
Salt-tolerant rhizobia isolated from a Tunisian oasis that are highly effective for symbiotic N2-fixation with Phaseolus vulgaris constitute a novel biovar (bv. mediterranense) of Sinorhizobium meliloti
journal, September 2006
- Mnasri, Bacem; Mrabet, Moncef; Laguerre, Gisèle
- Archives of Microbiology, Vol. 187, Issue 1
Nodule morphology, symbiotic specificity and association with unusual rhizobia are distinguishing features of the genus Listia within the southern African crotalarioid clade Lotononis s.l.
journal, May 2013
- Ardley, Julie K.; Reeve, Wayne G.; O'Hara, Graham W.
- Annals of Botany, Vol. 112, Issue 1
Characterization of root-nodulating bacteria associated to Prosopis farcta growing in the arid regions of Tunisia
journal, February 2011
- Fterich, A.; Mahdhi, M.; Caviedes, M. A.
- Archives of Microbiology, Vol. 193, Issue 6
Symbiotic diversity of Ensifer meliloti strains recovered from various legume species in Tunisia
journal, December 2009
- Mnasri, Bacem; Badri, Yazid; Saïdi, Sabrine
- Systematic and Applied Microbiology, Vol. 32, Issue 8
Symbiovars in rhizobia reflect bacterial adaptation to legumes
journal, April 2011
- Rogel, Marco A.; Ormeño-Orrillo, Ernesto; Martinez Romero, Esperanza
- Systematic and Applied Microbiology, Vol. 34, Issue 2
Phylogenetic position and revised classification ofAcacia s.l.(Fabaceae: Mimosoideae) in Africa, including new combinations inVachelliaandSenegalia
journal, May 2013
- Kyalangalilwa, Bruce; Boatwright, James S.; Daru, Barnabas H.
- Botanical Journal of the Linnean Society, Vol. 172, Issue 4
Sinorhizobium americanum symbiovar mediterranense is a predominant symbiont that nodulates and fixes nitrogen with common bean (Phaseolus vulgaris L.) in a Northern Tunisian field
journal, June 2012
- Mnasri, Bacem; Saïdi, Sabrine; Chihaoui, Saif-Allah
- Systematic and Applied Microbiology, Vol. 35, Issue 4
A Genomic Encyclopedia of the Root Nodule Bacteria: assessing genetic diversity through a systematic biogeographic survey
journal, February 2015
- Reeve, Wayne; Ardley, Julie; Tian, Rui
- Standards in Genomic Sciences, Vol. 10, Issue 1
Selection for acid tolerance inRhizobium meliloti
journal, September 1988
- Howieson, J. G.; Ewing, M. A.; D'Antuono, M. F.
- Plant and Soil, Vol. 105, Issue 2
R Factor Transfer in Rhizobium leguminosarum
journal, September 1974
- Beringer, J. E.
- Microbiology, Vol. 84, Issue 1
Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species
journal, November 2011
- Kim, O. -S.; Cho, Y. -J.; Lee, K.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 62, Issue Pt 3
The Genomes OnLine Database (GOLD) v.5: a metadata management system based on a four level (meta)genome project classification
journal, October 2014
- Reddy, T. B. K.; Thomas, Alex D.; Stamatis, Dimitri
- Nucleic Acids Research, Vol. 43, Issue D1
IMG 4 version of the integrated microbial genomes comparative analysis system
journal, October 2013
- Markowitz, Victor M.; Chen, I-Min A.; Palaniappan, Krishna
- Nucleic Acids Research, Vol. 42, Issue D1
The Fast Changing Landscape of Sequencing Technologies and Their Impact on Microbial Genome Assemblies and Annotation
journal, December 2012
- Mavromatis, Konstantinos; Land, Miriam L.; Brettin, Thomas S.
- PLoS ONE, Vol. 7, Issue 12
Constructs for insertional mutagenesis, transcriptional signal localization and gene regulation studies in root nodule and other bacteria
journal, June 1999
- Reeve, W. G.; Tiwari, R. P.; Worsley, P. S.
- Microbiology, Vol. 145, Issue 6
Velvet: Algorithms for de novo short read assembly using de Bruijn graphs
journal, February 2008
- Zerbino, D. R.; Birney, E.
- Genome Research, Vol. 18, Issue 5
High-quality draft assemblies of mammalian genomes from massively parallel sequence data
journal, December 2010
- Gnerre, S.; MacCallum, I.; Przybylski, D.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 4
Prodigal: prokaryotic gene recognition and translation initiation site identification
journal, March 2010
- Hyatt, Doug; Chen, Gwo-Liang; LoCascio, Philip F.
- BMC Bioinformatics, Vol. 11, Issue 1
The DOE-JGI Standard Operating Procedure for the Annotations of Microbial Genomes
journal, July 2009
- Mavromatis, Konstantinos; Ivanova, Natalia N.; Chen, I-Min A.
- Standards in Genomic Sciences, Vol. 1, Issue 1
Improving Microbial Genome Annotations in an Integrated Database Context
journal, February 2013
- Chen, I-Min A.; Markowitz, Victor M.; Chu, Ken
- PLoS ONE, Vol. 8, Issue 2
tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence
journal, March 1997
- Lowe, Todd M.; Eddy, Sean R.
- Nucleic Acids Research, Vol. 25, Issue 5
SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB
journal, November 2007
- Pruesse, E.; Quast, C.; Knittel, K.
- Nucleic Acids Research, Vol. 35, Issue 21
IMG ER: a system for microbial genome annotation expert review and curation
journal, June 2009
- Markowitz, Victor M.; Mavromatis, Konstantinos; Ivanova, Natalia N.
- Bioinformatics, Vol. 25, Issue 17
Polyphasic Taxonomy of Rhizobia: Emendation of the Genus Sinorhizobium and Description of Sinorhizobium meliloti comb. nov., Sinorhizobium saheli sp. nov., and Sinorhizobium teranga sp. nov.
journal, October 1994
- De Lajudie, P.; Willems, A.; Pot, B.
- International Journal of Systematic Bacteriology, Vol. 44, Issue 4
Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.
journal, August 2009
- Merabet, C.; Martens, M.; Mahdhi, M.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 60, Issue 3
Draft Genome Sequence of the Bean-Nodulating Sinorhizobium fredii Strain GR64
journal, December 2012
- Torres Tejerizo, Gonzalo; Lozano, Luis; González, Víctor
- Journal of Bacteriology, Vol. 194, Issue 24
Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.)
journal, January 2012
- Ormeño-Orrillo, Ernesto; Menna, Pâmela; Almeida, Luiz Gonzaga P.
- BMC Genomics, Vol. 13, Issue 1
MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods
journal, May 2011
- Tamura, K.; Peterson, D.; Peterson, N.
- Molecular Biology and Evolution, Vol. 28, Issue 10
Confidence Limits on Phylogenies: An Approach Using the Bootstrap
journal, July 1985
- Felsenstein, Joseph
- Evolution, Vol. 39, Issue 4, p. 783-791
The minimum information about a genome sequence (MIGS) specification
journal, May 2008
- Field, Dawn; Garrity, George; Gray, Tanya
- Nature Biotechnology, Vol. 26, Issue 5
The Genomic Standards Consortium
journal, June 2011
- Field, Dawn; Amaral-Zettler, Linda; Cochrane, Guy
- PLoS Biology, Vol. 9, Issue 6
Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.
journal, June 1990
- Woese, C. R.; Kandler, O.; Wheelis, M. L.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 12
Gene Ontology: tool for the unification of biology
journal, May 2000
- Ashburner, Michael; Ball, Catherine A.; Blake, Judith A.
- Nature Genetics, Vol. 25, Issue 1