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Title: A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome

Abstract

Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a chromosome-by-chromosome shotgun assembly. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population.

Authors:
 [1];  [2];  [3];  [1];  [4];  [5];  [5];  [6];  [7];  [3];  [6];  [4];  [2];  [8];  [9];  [10];  [2];  [11]
  1. Department of Energy Joint Genome Institute, Walnut Creek, CA (United States)
  2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben (Germany)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
  5. Univ. of California, Berkeley, CA (United States)
  6. Department of Energy Joint Genome Institute, Walnut Creek, CA (United States); HudsonAlpha Institute of Biotechnology, Huntsville, AL (United States)
  7. Kansas State Univ., Manhattan, KS (United States); South Dakota State Univ., Brookings, SD (United States)
  8. Univ. of Dundee & The James Hutton Institute, Dundee (United Kingdom)
  9. Kansas State Univ., Manhattan, KS (United States)
  10. Univ. of Minnesota, St. Paul, MN (United States)
  11. Department of Energy Joint Genome Institute, Walnut Creek, CA (United States); Univ. of California, Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1256489
Alternate Identifier(s):
OSTI ID: 1524013
Grant/Contract Number:  
AC02-05CH11231; SC0008700
Resource Type:
Accepted Manuscript
Journal Name:
GenomeBiology.com
Additional Journal Information:
Journal Volume: 16; Journal Issue: 1; Journal ID: ISSN 1465-6906
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chapman, Jarrod A., Mascher, Martin, Buluc, Aydin, Barry, Kerrie, Georganas, Evangelos, Session, Adam, Strnadova, Veronika, Jenkins, Jerry, Sehgal, Sunish, Oliker, Leonid, Schmutz, Jeremy, Yelick, Katherine A., Scholz, Uwe, Waugh, Robbie, Poland, Jesse A., Muehlbauer, Gary J., Stein, Nils, and Rokhsar, Daniel S. A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome. United States: N. p., 2015. Web. doi:10.1186/s13059-015-0582-8.
Chapman, Jarrod A., Mascher, Martin, Buluc, Aydin, Barry, Kerrie, Georganas, Evangelos, Session, Adam, Strnadova, Veronika, Jenkins, Jerry, Sehgal, Sunish, Oliker, Leonid, Schmutz, Jeremy, Yelick, Katherine A., Scholz, Uwe, Waugh, Robbie, Poland, Jesse A., Muehlbauer, Gary J., Stein, Nils, & Rokhsar, Daniel S. A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome. United States. https://doi.org/10.1186/s13059-015-0582-8
Chapman, Jarrod A., Mascher, Martin, Buluc, Aydin, Barry, Kerrie, Georganas, Evangelos, Session, Adam, Strnadova, Veronika, Jenkins, Jerry, Sehgal, Sunish, Oliker, Leonid, Schmutz, Jeremy, Yelick, Katherine A., Scholz, Uwe, Waugh, Robbie, Poland, Jesse A., Muehlbauer, Gary J., Stein, Nils, and Rokhsar, Daniel S. Sat . "A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome". United States. https://doi.org/10.1186/s13059-015-0582-8. https://www.osti.gov/servlets/purl/1256489.
@article{osti_1256489,
title = {A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome},
author = {Chapman, Jarrod A. and Mascher, Martin and Buluc, Aydin and Barry, Kerrie and Georganas, Evangelos and Session, Adam and Strnadova, Veronika and Jenkins, Jerry and Sehgal, Sunish and Oliker, Leonid and Schmutz, Jeremy and Yelick, Katherine A. and Scholz, Uwe and Waugh, Robbie and Poland, Jesse A. and Muehlbauer, Gary J. and Stein, Nils and Rokhsar, Daniel S.},
abstractNote = {Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a chromosome-by-chromosome shotgun assembly. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population.},
doi = {10.1186/s13059-015-0582-8},
journal = {GenomeBiology.com},
number = 1,
volume = 16,
place = {United States},
year = {Sat Jan 31 00:00:00 EST 2015},
month = {Sat Jan 31 00:00:00 EST 2015}
}

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Meraculous: De Novo Genome Assembly with Short Paired-End Reads
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Ensembl Genomes 2016: more genomes, more complexity
journal, November 2015

  • Kersey, Paul Julian; Allen, James E.; Armean, Irina
  • Nucleic Acids Research, Vol. 44, Issue D1
  • DOI: 10.1093/nar/gkv1209

Shifting the limits in wheat research and breeding using a fully annotated reference genome
journal, August 2018


A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene
journal, October 2019

  • Gardiner, Laura-Jayne; Bansept-Basler, Pauline; El-Soda, Mohamed
  • PLOS ONE
  • DOI: 10.1101/793604

Multi-Locus Genome Wide Association Mapping for Yield and Its Contributing Traits in Hexaploid Wheat under Different Water Regimes
journal, December 2019


Association of KASP markers with Hessian fly resistance in wheat of diverse origin
journal, July 2018


Hidden variation in polyploid wheat drives local adaptation
journal, December 2017

  • Gardiner, Laura-Jayne; Joynson, Ryan; Omony, Jimmy
  • Genome Research
  • DOI: 10.1101/217828

Exome sequencing of bulked segregants identified a novel TaMKK3-A allele linked to the wheat ERA8 ABA-hypersensitive germination phenotype
journal, January 2020

  • Martinez, Shantel A.; Shorinola, Oluwayesi; Conselman, Samantha
  • Theoretical and Applied Genetics, Vol. 133, Issue 3
  • DOI: 10.1007/s00122-019-03503-0

Genomic and pedigree-based prediction for leaf, stem, and stripe rust resistance in wheat
journal, April 2017

  • Juliana, Philomin; Singh, Ravi P.; Singh, Pawan K.
  • Theoretical and Applied Genetics, Vol. 130, Issue 7
  • DOI: 10.1007/s00122-017-2897-1

Genetic modification of spikelet arrangement in wheat increases grain number without significantly affecting grain weight
journal, December 2018

  • Wolde, Gizaw M.; Mascher, Martin; Schnurbusch, Thorsten
  • Molecular Genetics and Genomics, Vol. 294, Issue 2
  • DOI: 10.1007/s00438-018-1523-5

A genome-wide survey of DNA methylation in hexaploid wheat
journal, December 2015


QTL mapping of pre-harvest sprouting resistance in a white wheat cultivar Danby
journal, June 2018

  • Shao, Mingqin; Bai, Guihua; Rife, Trevor W.
  • Theoretical and Applied Genetics, Vol. 131, Issue 8
  • DOI: 10.1007/s00122-018-3107-5

Epistatic determinism of durum wheat resistance to the wheat spindle streak mosaic virus
journal, April 2017

  • Holtz, Yan; Bonnefoy, Michel; Viader, Véronique
  • Theoretical and Applied Genetics, Vol. 130, Issue 7
  • DOI: 10.1007/s00122-017-2904-6

Hidden variation in polyploid wheat drives local adaptation
journal, August 2018

  • Gardiner, Laura-Jayne; Joynson, Ryan; Omony, Jimmy
  • Genome Research, Vol. 28, Issue 9
  • DOI: 10.1101/gr.233551.117

Genotyping-by-sequencing approaches to characterize crop genomes: choosing the right tool for the right application
journal, January 2017

  • Scheben, Armin; Batley, Jacqueline; Edwards, David
  • Plant Biotechnology Journal, Vol. 15, Issue 2
  • DOI: 10.1111/pbi.12645

Strategies for Selecting Crosses Using Genomic Prediction in Two Wheat Breeding Programs
journal, July 2017


Fine mapping of the wheat powdery mildew resistance gene Pm52 using comparative genomics analysis and the Chinese Spring reference genomic sequence
journal, February 2019


Chromosome-scale comparative sequence analysis unravels molecular mechanisms of genome dynamics between two wheat cultivars
journal, August 2018


CerealsDB 3.0: expansion of resources and data integration
journal, June 2016

  • Wilkinson, Paul A.; Winfield, Mark O.; Barker, Gary L. A.
  • BMC Bioinformatics, Vol. 17, Issue 1
  • DOI: 10.1186/s12859-016-1139-x

Durum wheat genome highlights past domestication signatures and future improvement targets
journal, April 2019


Genetic analysis of wheat sensitivity to the ToxB fungal effector from Pyrenophora tritici-repentis, the causal agent of tan spot
text, January 2020

  • Corsi, Beatrice; Percival-Alwyn, Lawrence; Downie, Rowena C.
  • Apollo - University of Cambridge Repository
  • DOI: 10.17863/cam.53501

SNP-based pool genotyping and haplotype analysis accelerate fine-mapping of the wheat genomic region containing stripe rust resistance gene Yr26
journal, April 2018

  • Wu, Jianhui; Zeng, Qingdong; Wang, Qilin
  • Theoretical and Applied Genetics, Vol. 131, Issue 7
  • DOI: 10.1007/s00122-018-3092-8

Genome-Wide Association Study of Grain Architecture in Wild Wheat Aegilops tauschii
journal, May 2017


Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.
journal, April 2017

  • Beier, Sebastian; Himmelbach, Axel; Colmsee, Christian
  • Scientific Data, Vol. 4, Issue 1
  • DOI: 10.1038/sdata.2017.44

Genotyping-by-Sequencing Derived High-Density Linkage Map and its Application to QTL Mapping of Flag Leaf Traits in Bread Wheat
journal, November 2017


From markers to genome-based breeding in wheat
journal, January 2019


The improved assembly of 7DL chromosome provides insight into the structure and evolution of bread wheat
journal, September 2019

  • Feng, Kewei; Cui, Licao; Wang, Le
  • Plant Biotechnology Journal, Vol. 18, Issue 3
  • DOI: 10.1111/pbi.13240

Genome-Wide Association Mapping of Grain Micronutrients Concentration in Aegilops tauschii
journal, February 2019


Broadening the bread wheat D genome
journal, February 2019


Genome-wide association mapping for resistance to leaf rust, stripe rust and tan spot in wheat reveals potential candidate genes
journal, March 2018

  • Juliana, Philomin; Singh, Ravi P.; Singh, Pawan K.
  • Theoretical and Applied Genetics, Vol. 131, Issue 7
  • DOI: 10.1007/s00122-018-3086-6

Heat in Wheat: Exploit Reverse Genetic Techniques to Discover New Alleles Within the Triticum durum sHsp26 Family
journal, September 2018

  • Comastri, Alessia; Janni, Michela; Simmonds, James
  • Frontiers in Plant Science, Vol. 9
  • DOI: 10.3389/fpls.2018.01337

Dense genotyping-by-sequencing linkage maps of two Synthetic W7984×Opata reference populations provide insights into wheat structural diversity
journal, February 2019


Mapping and KASP marker development for wheat curl mite resistance in “TAM 112” wheat using linkage and association analysis
journal, September 2018


An ancestral NB-LRR with duplicated 3′UTRs confers stripe rust resistance in wheat and barley
journal, September 2019


Breeding-assisted genomics: Applying meta-GWAS for milling and baking quality in CIMMYT wheat breeding program
journal, November 2018


Poaceae-specific MS1 encodes a phospholipid-binding protein for male fertility in bread wheat
journal, November 2017

  • Wang, Zheng; Li, Jian; Chen, Shaoxia
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 47
  • DOI: 10.1073/pnas.1715570114

Genetic insights into morphometric inflorescence traits of wheat
journal, February 2019

  • Wolde, Gizaw M.; Trautewig, Corinna; Mascher, Martin
  • Theoretical and Applied Genetics, Vol. 132, Issue 6
  • DOI: 10.1007/s00122-019-03305-4

High throughput SNP discovery and genotyping in hexaploid wheat
journal, January 2018


Genetic Mapping of Millions of SNPs in Safflower ( Carthamus tinctorius L.) via Whole-Genome Resequencing
journal, May 2016

  • Bowers, John E.; Pearl, Stephanie A.; Burke, John M.
  • G3: Genes|Genomes|Genetics, Vol. 6, Issue 7
  • DOI: 10.1534/g3.115.026690

Genetic diversity and relationship between domesticated rye and its wild relatives as revealed through genotyping‐by‐sequencing
journal, March 2018

  • Schreiber, Mona; Himmelbach, Axel; Börner, Andreas
  • Evolutionary Applications, Vol. 12, Issue 1
  • DOI: 10.1111/eva.12624

Chromosome-specific sequencing reveals an extensive dispensable genome component in wheat
journal, November 2016

  • Liu, Miao; Stiller, Jiri; Holušová, Kateřina
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep36398

High-resolution mapping of the pericentromeric region on wheat chromosome arm 5AS harbouring the Fusarium head blight resistance QTL Qfhs.ifa-5A
journal, November 2017

  • Buerstmayr, Maria; Steiner, Barbara; Wagner, Christian
  • Plant Biotechnology Journal, Vol. 16, Issue 5
  • DOI: 10.1111/pbi.12850

Unraveling the genetic architecture of grain size in einkorn wheat through linkage and homology mapping and transcriptomic profiling
journal, June 2019

  • Yu, Kang; Liu, Dongcheng; Chen, Yong
  • Journal of Experimental Botany, Vol. 70, Issue 18
  • DOI: 10.1093/jxb/erz247

Assembly of allele-aware, chromosomal-scale autopolyploid genomes based on Hi-C data
journal, August 2019


Chromosome-scale comparative sequence analysis unravels molecular mechanisms of genome dynamics between two wheat cultivars
text, January 2018

  • Thind, Anupriya Kaur; Wicker, Thomas; Müller, Thomas
  • BioMed Central
  • DOI: 10.5167/uzh-160628

Temperature‐sensitive wheat stem rust resistance gene Sr15 is effective against Puccinia graminis f. sp. tritici race TTKSK
journal, August 2018

  • Gao, L.; Babiker, E. M.; Nava, I. C.
  • Plant Pathology, Vol. 68, Issue 1
  • DOI: 10.1111/ppa.12928

Genotyping by Sequencing Using Specific Allelic Capture to Build a High-Density Genetic Map of Durum Wheat
journal, May 2016


Comparative structural analysis of Bru1 region homeologs in Saccharum spontaneum and S. officinarum
journal, June 2016


Genome-wide characterization of microsatellites in Triticeae species: abundance, distribution and evolution
journal, August 2016

  • Deng, Pingchuan; Wang, Meng; Feng, Kewei
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep32224

Genomics as the key to unlocking the polyploid potential of wheat
journal, June 2015

  • Borrill, Philippa; Adamski, Nikolai; Uauy, Cristobal
  • New Phytologist, Vol. 208, Issue 4
  • DOI: 10.1111/nph.13533

A High-Density Consensus Map of Common Wheat Integrating Four Mapping Populations Scanned by the 90K SNP Array
journal, August 2017


Wheat ms5 male‐sterility is induced by recessive homoeologous A and D genome non‐specific lipid transfer proteins
journal, June 2019

  • Pallotta, Margaret A.; Warner, Patricia; Kouidri, Allan
  • The Plant Journal, Vol. 99, Issue 4
  • DOI: 10.1111/tpj.14350

De novo hybrid assembly of the rubber tree genome reveals evidence of paleotetraploidy in Hevea species
journal, February 2017

  • Pootakham, Wirulda; Sonthirod, Chutima; Naktang, Chaiwat
  • Scientific Reports, Vol. 7, Issue 1
  • DOI: 10.1038/srep41457

Genome‐Wide Association and Prediction of Grain and Semolina Quality Traits in Durum Wheat Breeding Populations
journal, November 2017


Forward genetics using radiation hybrids (deletion mutants) in plants
journal, November 2018


Genomic Selection for Processing and End-Use Quality Traits in the CIMMYT Spring Bread Wheat Breeding Program
journal, July 2016


The high-quality genome of Brassica napus cultivar ‘ZS11’ reveals the introgression history in semi-winter morphotype
journal, October 2017

  • Sun, Fengming; Fan, Guangyi; Hu, Qiong
  • The Plant Journal, Vol. 92, Issue 3
  • DOI: 10.1111/tpj.13669

Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome
journal, August 2018

  • Keeble-Gagnère, Gabriel; Rigault, Philippe; Tibbits, Josquin
  • Genome Biology, Vol. 19, Issue 1
  • DOI: 10.1186/s13059-018-1475-4

Genomic approaches for studying crop evolution
journal, September 2018


Imputation accuracy of wheat genotyping-by-sequencing (GBS) data using barley and wheat genome references
journal, January 2019


Features of the organization of bread wheat chromosome 5BS based on physical mapping
journal, February 2018


A modified sequence capture approach allowing standard and methylation analyses of the same enriched genomic DNA sample
journal, April 2018


Selection and evaluation of reference genes for qRT-PCR analysis in Euscaphis konishii Hayata based on transcriptome data
journal, June 2018


Genetic insights into morphometric inflorescence traits of wheat
journal, February 2019

  • Wolde, Gizaw M.; Trautewig, Corinna; Mascher, Martin
  • Theoretical and Applied Genetics, Vol. 132, Issue 6
  • DOI: 10.1007/s00122-019-03305-4

Mapping causal mutations by exome sequencing in a wheat TILLING population: a tall mutant case study
journal, November 2017

  • Mo, Youngjun; Howell, Tyson; Vasquez-Gross, Hans
  • Molecular Genetics and Genomics, Vol. 293, Issue 2
  • DOI: 10.1007/s00438-017-1401-6

Developing integrated crop knowledge networks to advance candidate gene discovery
journal, December 2016

  • Hassani-Pak, Keywan; Castellote, Martin; Esch, Maria
  • Applied & Translational Genomics, Vol. 11
  • DOI: 10.1016/j.atg.2016.10.003

An ancestral NB-LRR with duplicated 3′UTRs confers stripe rust resistance in wheat and barley
journal, September 2019


Genotyping-by-Sequencing Derived High-Density Linkage Map and its Application to QTL Mapping of Flag Leaf Traits in Bread Wheat
journal, November 2017


Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
journal, May 2018


Dense genotyping-by-sequencing linkage maps of two Synthetic W7984×Opata reference populations provide insights into wheat structural diversity
journal, February 2019


RNAseq analysis reveals drought-responsive molecular pathways with candidate genes and putative molecular markers in root tissue of wheat
journal, September 2019


De novo hybrid assembly of the rubber tree genome reveals evidence of paleotetraploidy in Hevea species
journal, February 2017

  • Pootakham, Wirulda; Sonthirod, Chutima; Naktang, Chaiwat
  • Scientific Reports, Vol. 7, Issue 1
  • DOI: 10.1038/srep41457

Gramene 2016: comparative plant genomics and pathway resources
journal, November 2015

  • Tello-Ruiz, Marcela K.; Stein, Joshua; Wei, Sharon
  • Nucleic Acids Research, Vol. 44, Issue D1
  • DOI: 10.1093/nar/gkv1179

pBACode: a random-barcode-based high-throughput approach for BAC paired-end sequencing and physical clone mapping
journal, December 2016


A modified sequence capture approach allowing standard and methylation analyses of the same enriched genomic DNA sample
journal, November 2017

  • Olohan, Lisa; Gardiner, Laura-Jayne; Lucaci, Anita
  • BMC Genomics
  • DOI: 10.1101/209585

Genetic diversity and relationship between domesticated rye and its wild relatives as revealed through genotyping‐by‐sequencing
journal, March 2018

  • Schreiber, Mona; Himmelbach, Axel; Börner, Andreas
  • Evolutionary Applications, Vol. 12, Issue 1
  • DOI: 10.1111/eva.12624

Dominant inhibition of awn development by a putative zinc‐finger transcriptional repressor expressed at the B1 locus in wheat
journal, October 2019

  • Huang, Daiqing; Zheng, Qian; Melchkart, Tancey
  • New Phytologist, Vol. 225, Issue 1
  • DOI: 10.1111/nph.16154

A highly recombined, high-density, eight-founder wheat MAGIC map reveals extensive segregation distortion and genomic locations of introgression segments
journal, January 2016

  • Gardner, Keith A.; Wittern, Lukas M.; Mackay, Ian J.
  • Plant Biotechnology Journal, Vol. 14, Issue 6
  • DOI: 10.1111/pbi.12504

Multiplex sequencing of bacterial artificial chromosomes for assembling complex plant genomes
journal, January 2016

  • Beier, Sebastian; Himmelbach, Axel; Schmutzer, Thomas
  • Plant Biotechnology Journal, Vol. 14, Issue 7
  • DOI: 10.1111/pbi.12511

BioNano genome mapping of individual chromosomes supports physical mapping and sequence assembly in complex plant genomes
journal, January 2016

  • Staňková, Helena; Hastie, Alex R.; Chan, Saki
  • Plant Biotechnology Journal, Vol. 14, Issue 7
  • DOI: 10.1111/pbi.12513

Thein silicoidentification and characterization of a bread wheat/Triticum militinaeintrogression line
journal, September 2016

  • Abrouk, Michael; Balcárková, Barbora; Šimková, Hana
  • Plant Biotechnology Journal, Vol. 15, Issue 2
  • DOI: 10.1111/pbi.12610

The improved assembly of 7DL chromosome provides insight into the structure and evolution of bread wheat
journal, September 2019

  • Feng, Kewei; Cui, Licao; Wang, Le
  • Plant Biotechnology Journal, Vol. 18, Issue 3
  • DOI: 10.1111/pbi.13240

The genetic architecture of genome‐wide recombination rate variation in allopolyploid wheat revealed by nested association mapping
journal, July 2018

  • Jordan, Katherine W.; Wang, Shichen; He, Fei
  • The Plant Journal, Vol. 95, Issue 6
  • DOI: 10.1111/tpj.14009

CerealsDB 3.0: expansion of resources and data integration
journal, June 2016

  • Wilkinson, Paul A.; Winfield, Mark O.; Barker, Gary L. A.
  • BMC Bioinformatics, Vol. 17, Issue 1
  • DOI: 10.1186/s12859-016-1139-x

Selection and evaluation of reference genes for qRT-PCR analysis in Euscaphis konishii Hayata based on transcriptome data
journal, June 2018


Genotyping by Sequencing Using Specific Allelic Capture to Build a High-Density Genetic Map of Durum Wheat
journal, May 2016


Nested Association Mapping of Stem Rust Resistance in Wheat Using Genotyping by Sequencing
journal, May 2016


Breeding-assisted genomics: Applying meta-GWAS for milling and baking quality in CIMMYT wheat breeding program
journal, November 2018


Genetic Mapping of Millions of SNPs in Safflower ( Carthamus tinctorius L.) via Whole-Genome Resequencing
journal, May 2016

  • Bowers, John E.; Pearl, Stephanie A.; Burke, John M.
  • G3: Genes|Genomes|Genetics, Vol. 6, Issue 7
  • DOI: 10.1534/g3.115.026690

Genomic Selection in Preliminary Yield Trials in a Winter Wheat Breeding Program
journal, June 2018

  • Belamkar, Vikas; Guttieri, Mary J.; Hussain, Waseem
  • G3: Genes|Genomes|Genetics, Vol. 8, Issue 8
  • DOI: 10.1534/g3.118.200415

A High Resolution Radiation Hybrid Map of Wheat Chromosome 4A
journal, January 2017

  • Balcárková, Barbora; Frenkel, Zeev; Škopová, Monika
  • Frontiers in Plant Science, Vol. 7
  • DOI: 10.3389/fpls.2016.02063

Genome-Wide Association Study of Grain Architecture in Wild Wheat Aegilops tauschii
journal, May 2017


Genotyping-by-Sequencing (GBS) Revealed Molecular Genetic Diversity of Iranian Wheat Landraces and Cultivars
journal, August 2017

  • Alipour, Hadi; Bihamta, Mohammad R.; Mohammadi, Valiollah
  • Frontiers in Plant Science, Vol. 8
  • DOI: 10.3389/fpls.2017.01293

A High-Density Consensus Map of Common Wheat Integrating Four Mapping Populations Scanned by the 90K SNP Array
journal, August 2017


Genome-Wide Association Mapping of Grain Micronutrients Concentration in Aegilops tauschii
journal, February 2019


Uncovering Genomic Regions Associated With 36 Agro-Morphological Traits in Indian Spring Wheat Using GWAS
journal, April 2019

  • Sheoran, Sonia; Jaiswal, Sarika; Kumar, Deepender
  • Frontiers in Plant Science, Vol. 10
  • DOI: 10.3389/fpls.2019.00527

Insight Into the Prospects for the Improvement of Seed Starch in Legume—A Review
journal, October 2019

  • Tayade, Rupesh; Kulkarni, Krishnanand P.; Jo, Hyun
  • Frontiers in Plant Science, Vol. 10
  • DOI: 10.3389/fpls.2019.01213

Next-Generation Sequencing — An Overview of the History, Tools, and “Omic” Applications
book, January 2016

  • Kulski, Jerzy K.
  • Next Generation Sequencing - Advances, Applications and Challenges
  • DOI: 10.5772/61964