Title: Metabolomics reveals metabolic changes in male reproductive cells exposed to thirdhand smoke

Abstract

Thirdhand smoke (THS) is a new term for the toxins in cigarette smoke that linger in the environment long after the cigarettes are extinguished. The effects of THS exposure on male reproduction have not yet been studied. In this study, metabolic changes in male germ cell lines (GC-2 and TM-4) were analyzed after THS treatment for 24 h. THS-loaded chromatography paper samples were generated in a laboratory chamber system and extracted in DMEM. At a paper: DMEM ratio of 50 μg/ml, cell viability in both cell lines was normal, as measured by the MTT assay and markers of cytotoxicity, cell cycle, apoptosis and ROS production were normal as measured by quantitative immunofluorescence. Metabolomic analysis was performed on methanol extracts of GC-2 and TM-4 cells. Glutathione metabolism in GC-2 cells, and nucleic acid and ammonia metabolism in TM-4 cells, was changed significantly by THS treatment. RT-PCR analyses of mRNA for enzyme genes Gss and Ggt in GC-2 cells, and TK, SMS and Glna in TM-4 cells reinforced these findings, showing changes in the levels of enzymes involved in the relevant pathways. In conclusion, exposure to THS at very low concentrations caused distinct metabolic changes in two different types of male reproductivemore » cell lines.« less

Authors:

Xu, Bo ^[1]; Chen, Minjian ^[1]; Yao, Mengmeng ^[1]; Ji, Xiaoli ^[1]; Mao, Zhilei ^[1]; Tang, Wei ^[1]; Qiao, Shanlei ^[1]; Schick, Suzaynn F. ^[2]; Mao, Jian-Hua ^[3]; Hang, Bo ^[3]; Xia, Yankai ^[1]

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+ Show Author Affiliations

1. Nanjing Medical Univ., Nanjing (China)
2. Univ. of California, San Francisco, CA (United States)
3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division

Publication Date:

Thu Oct 22 00:00:00 EDT 2015

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE Office of Science (SC)

OSTI Identifier:

1256060

Alternate Identifier(s):

OSTI ID: 1378601

Grant/Contract Number:  

AC02-05CH11231

Resource Type:

Accepted Manuscript

Journal Name:

Scientific Reports

Additional Journal Information:

Journal Volume: 5; Journal ID: ISSN 2045-2322

Publisher:

Nature Publishing Group

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES